Difference between revisions of "Team:Manchester/Parts"

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{{Manchester}}
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{{Manchester/CSS}}
 
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<style>
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.auto-style1{
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    width:75%;
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    margin:auto;
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table tr th{
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th, td{
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<br><br>
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<h1 class="title11">Parts</h1>
  
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<center>
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<table cellpadding="5" style="margin-bottom:150px;" class="auto-style1">
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<tr>
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<th style="border:1px black solid;background: #add8e6;">BioBrick</th>
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<th style="border:1px black solid;background: #add8e6;">Link</th>
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<th style="border:1px black solid;background: #add8e6;">Part Name</th>
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<th style="border:1px black solid;background: #add8e6;">Description</th>
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<th style="border:1px black solid;background: #add8e6;">Part Type</th>
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</tr>
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<tr>
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<td style="border:1px black solid;">BBa_K2092000</td>
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<td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K2092000" target="_blank"> http://parts.igem.org/Part:BBa_K2092000</td>
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<td style="border:1px black solid;">AOx (without RBS)</td>
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<td style="border:1px black solid;">
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This part encodes for the  AOx1 protein from <i>Pichia pastoris</i>. This enzyme oxidises primary alcohols to produce aldehydes and hydrogen peroxide. It is designed with an NdeI restriction site between the prefix and the start of the AOx gene.
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</td>
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<td style="border:1px black solid;">Basic</td>
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</tr>
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<tr>
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<td style="border:1px black solid;">BBa_K2092001</td>
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<td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K2092001" target="_blank">http://parts.igem.org/Part:BBa_K2092001 </a></td>
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<td style="border:1px black solid;">alcR (without RBS/scar)</td>
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<td style="border:1px black solid;">This part encodes for AlcR protein, an ethanol-activated transcriptional factor. It binds to alcA promoter and aldA promoter to induce expression of proteins in the presence of ethanol or threonine. </td>
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<td style="border:1px black solid;">Basic</td>
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</tr>
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<tr>
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<td style="border:1px black solid;">BBa_K2092002</td>
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<td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K2092002 " target="_blank">http://parts.igem.org/Part:BBa_K2092002 </a></td>
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<td style="border:1px black solid;">P<i>alcA</i></td>
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<td style="border:1px black solid;">This part contains the ethanol/alcR regulated  alcA promoter, the native variant of alcA promoter from Aspergillus nidulans which tightly regulates gene expression. It is an improved BioBrick from <a href="http://parts.igem.org/Part:BBa_K678001" target="_blank"> iGEM DTU-Denmark 2011</a> </td>
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<td style="border:1px black solid;">Basic</td>
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</tr>
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<tr>
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<td style="border:1px black solid;">BBa_K2092003</td>
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<td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K2092003" target="_blank">http://parts.igem.org/Part:BBa_K2092003 </a></td>
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<td style="border:1px black solid;">P<i>alcA(var)</i></td>
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<td style="border:1px black solid;">This part contains the ethanol/alcR regulated alcA promoter, an engineered variant of the alcA promoter from Aspergillus nidulans which tightly regulates gene expression.  The lack of one binding site on this promoter is suggested to have 70% decrease in promoter strength in comparison to the native <a href="http://parts.igem.org/Part:BBa_K2092002" target="_blank"> P<i>alcA</i></a>.</td>
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<td style="border:1px black solid;">Basic</td>
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</tr>
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<tr>
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<td style="border:1px black solid;">BBa_K2092004</td>
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<td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K2092004" target="_blank">http://parts.igem.org/Part:BBa_K2092004</a></td>
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<td style="border:1px black solid;">alcR (incl. RBS)</td>
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<td style="border:1px black solid;">This part is a twin of <a href="http://parts.igem.org/Part:BBa_K2092001" target="_blank">BBa_K2092001</a>  with an additional <a href="http://parts.igem.org/Part:BBa_J34801" target="_blank"> RBS </a>  upstream of the start codon.  This part encodes for AlcR protein, an ethanol-activated transcriptional factor from <i>Aspergillus nidulans</i>. It binds to <a href="http://parts.igem.org/Part:BBa_K2092003" target="_blank">alcA</a> and aldA promoters to induce expression of proteins in the presence of ethanol or threonine.</td>
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<td style="border:1px black solid;">Composite</td>
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</tr>
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<tr>
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<td style="border:1px black solid;">BBa_K2092006</td>
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<td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K2092006" target="_balnk">http://parts.igem.org/Part:BBa_K2092006</a></td>
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<td style="border:1px black solid;">Pcon+RBS+amilCP</td>
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<td style="border:1px black solid;">This part consists of a <a href="http://parts.igem.org/Part:BBa_J23110" target="_blank"> constitutive promoter</a>, <a href="http://parts.igem.org/Part:BBa_B0034" target="_blank"> RBS</a> and <a href="http://parts.igem.org/Part:BBa_K592009" target="_blank"> blue chromoprotein amilCP</a>.  Suitable to be used as a reporter.</td>
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<td style="border:1px black solid;">Composite</td>
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</tr>
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<tr>
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<td style="border:1px black solid;">BBa_K2092008</td>
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<td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K2092008" target="_blank">http://parts.igem.org/Part:BBa_K2092008</a></td>
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<td style="border:1px black solid;">Pcon+RBS+alcR</td>
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<td style="border:1px black solid;">This part consists of a <a href="http://parts.igem.org/Part:BBa_J23110" target="_blank"> constitutive promoter</a>, <a href="http://parts.igem.org/Part:BBa_B0034" target="_blank"> RBS</a> and <a href="http://parts.igem.org/Part:BBa_K2092001" target="_blank"> alcR</a>.  Suitable to be used to measure the promoter strengths of <a href="http://partsregistry.org/Part:BBa_K592002" target="_blank">alcA</a>/aldA.</td>
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<td style="border:1px black solid;">Composite</td>
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</tr>
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<tr>
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<td style="border:1px black solid;">BBa_K2092009</td>
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<td style="border:1px black solid;"><a href="
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http://parts.igem.org/Part:BBa_K2092009"target="_blank">http://parts.igem.org/Part:BBa_K2092009</a></td>
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<td style="border:1px black solid;">P<i>alcA</i>(var)+RBS+amilCP</td>
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<td style="border:1px black solid;">
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This part consists of <a href="http://parts.igem.org/Part:BBa_K2092003" target="_blank">P<i>alcA</i>(var)</a> tagged with <a href="http://partsregistry.org/Part:BBa_K592009" target="_blank">blue chromoprotein amilCP</a>, which makes it capable to produce blue colour upon the binding of its ethanol-activated transcription factor <a href="http://partsregistry.org/Part:BBa_K592001" target="_blank">AlcR</a>.</td>
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<td style="border:1px black solid;">Composite</td>
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</tr>
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</table>
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</center>
  
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
 
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
 
  
  
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<div class="floatleft1 project">
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<a class="projectlink" href="https://2016.igem.org/Team:Manchester/Achievement"><< Achievement Overview</a>
 
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<div class="column half_size">
 
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<div class="highlight">
 
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<h5>Note</h5>
 
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
 
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<h5>Adding parts to the registry</h5>
 
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
 
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
 
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<h5>What information do I need to start putting my parts on the Registry?</h5>
 
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<p>The information needed to initially create a part on the Registry is:</p>
 
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<ul>
 
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<li>Part Name</li>
 
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<li>Part type</li>
 
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<li>Creator</li>
 
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<li>Sequence</li>
 
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<li>Short Description (60 characters on what the DNA does)</li>
 
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<li>Long Description (Longer description of what the DNA does)</li>
 
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<li>Design considerations</li>
 
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</ul>
 
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<p>
 
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
 
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<h5>Inspiration</h5>
 
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
 
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
 
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<ul>
 
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
 
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
 
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
 
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</ul>
 
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<h5>Part Table </h5>
 
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<groupparts>iGEM2016 Example</groupparts>
 
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{{Manchester/CSS/footer}}

Latest revision as of 02:24, 20 October 2016

Manchester iGEM 2016


Parts

BioBrick Link Part Name Description Part Type
BBa_K2092000 http://parts.igem.org/Part:BBa_K2092000 AOx (without RBS) This part encodes for the AOx1 protein from Pichia pastoris. This enzyme oxidises primary alcohols to produce aldehydes and hydrogen peroxide. It is designed with an NdeI restriction site between the prefix and the start of the AOx gene. Basic
BBa_K2092001 http://parts.igem.org/Part:BBa_K2092001 alcR (without RBS/scar) This part encodes for AlcR protein, an ethanol-activated transcriptional factor. It binds to alcA promoter and aldA promoter to induce expression of proteins in the presence of ethanol or threonine. Basic
BBa_K2092002 http://parts.igem.org/Part:BBa_K2092002 PalcA This part contains the ethanol/alcR regulated alcA promoter, the native variant of alcA promoter from Aspergillus nidulans which tightly regulates gene expression. It is an improved BioBrick from iGEM DTU-Denmark 2011 Basic
BBa_K2092003 http://parts.igem.org/Part:BBa_K2092003 PalcA(var) This part contains the ethanol/alcR regulated alcA promoter, an engineered variant of the alcA promoter from Aspergillus nidulans which tightly regulates gene expression. The lack of one binding site on this promoter is suggested to have 70% decrease in promoter strength in comparison to the native PalcA. Basic
BBa_K2092004 http://parts.igem.org/Part:BBa_K2092004 alcR (incl. RBS) This part is a twin of BBa_K2092001 with an additional RBS upstream of the start codon. This part encodes for AlcR protein, an ethanol-activated transcriptional factor from Aspergillus nidulans. It binds to alcA and aldA promoters to induce expression of proteins in the presence of ethanol or threonine. Composite
BBa_K2092006 http://parts.igem.org/Part:BBa_K2092006 Pcon+RBS+amilCP This part consists of a constitutive promoter, RBS and blue chromoprotein amilCP. Suitable to be used as a reporter. Composite
BBa_K2092008 http://parts.igem.org/Part:BBa_K2092008 Pcon+RBS+alcR This part consists of a constitutive promoter, RBS and alcR. Suitable to be used to measure the promoter strengths of alcA/aldA. Composite
BBa_K2092009 http://parts.igem.org/Part:BBa_K2092009 PalcA(var)+RBS+amilCP This part consists of PalcA(var) tagged with blue chromoprotein amilCP, which makes it capable to produce blue colour upon the binding of its ethanol-activated transcription factor AlcR. Composite
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