Difference between revisions of "Team:Hong Kong HKU/Parts"

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     <h3>Achievements</h3><br>
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       <li class="active"><a data-toggle="pill" href="#Parts">Parts</a></li>
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      <li><a data-toggle="pill" href="#Achievements">Achievements</a></li>
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       <a class="navbar-brand" href="#">HKU iGEM Team</a>
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      <li><a href="https://2016.igem.org/Team:Hong_Kong_HKU/Results">Results</a></li>
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      <li><a href="https://2016.igem.org/Team:Hong_Kong_HKU/Demonstrate">Demonstrate our work</a></li>
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       <li><a href="https://2016.igem.org/Team:Hong_Kong_HKU/Proof">Proof of Concept</a></li>
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     We spent the entire Summer in the lab, and such hard work resulted in some achievements.
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     We have submitted parts to the registry, experimentally validated our new BioBrick devices and demonstrated the assembly of our design and fulfilled the criteria for a gold medal.
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        <li class="active"><a href="https://2016.igem.org/Team:Hong_Kong_HKU">Homepage</a> </li>
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            <li><a href="https://2016.igem.org/Team:Hong_Kong_HKU/Description">Description</a> </li>
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        <h3>Parts</h3><hr><br>
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            <li><a href="https://2016.igem.org/Team:Hong_Kong_HKU/Design">Design</a> </li>
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            <li><a href="https://2016.igem.org/Team:Hong_Kong_HKU/Proof">Proof of concept</a> </li>
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            <li><a href="https://2016.igem.org/Team:Hong_Kong_HKU/Demonstrate">Demonstrate</a> </li>
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                  <h4 class="panel-title"><a data-toggle="collapse" data-parent="#PartsContent" href="#Part1"><h4>Biobricks</h4></a></h4>
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                <div id="Part1" class="panel-collapse collapse in">
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        <li class="dropdown"> <a href="https://2016.igem.org/Team:Hong_Kong_HKU/Notebook" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-expanded="false" aria-haspopup="true">Notebook<span class="caret"></span></a>
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            <li><a href="https://2016.igem.org/Team:Hong_Kong_HKU/Protocol">Protocol</a> </li>
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                  <font size="4"><b>Featured Parts</b></font><br>
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                  <font size="3">
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            <li><a href="https://2016.igem.org/Team:Hong_Kong_HKU/Results">Results</a> </li>
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                  <b><a href="http://parts.igem.org/Part:BBa_K2054000">BBa_K2054000</a> - ssDNAs of our nanostructure</b><br>
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                  Forwarding our all submitted parts, we agree that it would be better if there is mighty single biobrick that encodes the all components of our desired structure.
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                  And here we have it – putting all together . <br>
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        <li><a href="https://2016.igem.org/Team:Hong_Kong_HKU/Parts">Parts</a></li>
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                  <b><a href="http://parts.igem.org/Part:BBa_K2054006">BBa_K2054006</a> - ssDNAs of our simplified nanostructure</b><br>
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        <li><a href="https://2016.igem.org/Team:Hong_Kong_HKU/Human_Practices">Human Practices</a></li>
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                  Oligos 1 and 5 are in a single plasmid to encode for the simplified version of our nanostructure.<br><br>
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        <li><a href="https://2016.igem.org/Team:Hong_Kong_HKU/Attributions">Attributions</a></li>
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                  <font size="4"><b>Table of Parts</b></font><br>
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        <a href="https://www.facebook.com/igemhku2016" target="_blank"><img style="float:right" src="https://static.igem.org/mediawiki/2016/e/ea/T--Hong_Kong_HKU--FB.jpg" width="24px" height="24px" alt="Placeholder image"></a>
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                            <tr>
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                                <th style="text-align:center">Type</th>
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                                <th style="text-align:center">Name (Part number)</th>
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                                <th style="text-align:center">Type</th>
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                                <th style="text-align:center">Description</th>
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                                <th style="text-align:center">Length(bp)</th>
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                            <tr>
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                                <td style="text-align:center">Composite Part<br>(Featured)</td>
 +
                                <td style="text-align:center"><a href="http://parts.igem.org/Part:BBa_K2054000">BBa_K2054000</a></td>
 +
                                <td style="text-align:center">DNA</td>
 +
                                <td style="text-align:center">ALL oligos included in our desired nanostructure</td>
 +
                                <td style="text-align:center">3561</td>
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                            </tr>
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                            <tr>
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                                <td style="text-align:center">Basic Part</td>
 +
                                <td style="text-align:center"><a href="http://parts.igem.org/Part:BBa_K2054001">BBa_K2054001</a></td>
 +
                                <td style="text-align:center">DNA</td>
 +
                                <td style="text-align:center">Oligo 1 for the Tetrahedral nanostructure, w/ split G-quadruplex</td>
 +
                                <td style="text-align:center">2388</td>
 +
                            </tr>
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                            <tr>
 +
                                <td style="text-align:center">Basic Part</td>
 +
                                <td style="text-align:center"><a href="http://parts.igem.org/Part:BBa_K2054002">BBa_K2054002</a></td>
 +
                                <td style="text-align:center">DNA</td>
 +
                                <td style="text-align:center">Oligo 2 for the Tetrahedral nanostructure</td>
 +
                                <td style="text-align:center">2358</td>
 +
                            </tr>
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                            <tr>
 +
                                <td style="text-align:center">Basic Part</td>
 +
                                <td style="text-align:center"><a href="http://parts.igem.org/Part:BBa_K2054003">BBa_K2054003</a></td>
 +
                                <td style="text-align:center">DNA</td>
 +
                                <td style="text-align:center">Oligo 3 of the Tetrahedral nanostructure</td>
 +
                                <td style="text-align:center">2375</td>
 +
                            </tr>
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                            <tr>
 +
                                <td style="text-align:center">Basic Part</td>
 +
                                <td style="text-align:center"><a href="http://parts.igem.org/Part:BBa_K2054004">BBa_K2054004</a></td>
 +
                                <td style="text-align:center">DNA</td>
 +
                                <td style="text-align:center">Oligo 4 of the Tetrahedral nanostructure</td>
 +
                                <td style="text-align:center">2375</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td style="text-align:center">Basic Part</td>
 +
                                <td style="text-align:center"><a href="http://parts.igem.org/Part:BBa_K2054005">BBa_K2054005</a></td>
 +
                                <td style="text-align:center">DNA</td>
 +
                                <td style="text-align:center">Oligo 5 of the Tetrahedral nanostructure</td>
 +
                                <td style="text-align:center">2313</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td style="text-align:center">Composite Part<br>(Featured)</td>
 +
                                <td style="text-align:center"><a href="http://parts.igem.org/Part:BBa_K2054006">BBa_K2054006</a></td>
 +
                                <td style="text-align:center">DNA</td>
 +
                                <td style="text-align:center">Oligos of the simplified 2D nanostructure </td>
 +
                                <td style="text-align:center">2639</td>
 +
                            </tr>
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                    </table>
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    <h3>Achievements</h3>
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            <th style="text-align:center" colspan="2">For Bronze Medal</th>
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            </tr>
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<img class="img-responsive center-block" src="https://static.igem.org/mediawiki/2016/a/a8/T--Hong_Kong_HKU--Logo_HKU_2460x1860.jpg" style="width:240px; height:180px;" alt="Placeholder image">
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<div class="container" align="center">
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<h3>Parts</h3>
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            <th style="text-align:center">✓</th>
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                <td>Team Registration and Giant Jamboree Attendance (where we have prepared a 20-minutes presentation and a poster)</td>
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<ul class="nav nav-pills">
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  <li class="active"><a data-toggle="pill" href="#Basicpart">Basic Part</a></li>
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        <tr>
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  <li><a data-toggle="pill" href="#Compositepart">Composite Part</a></li>
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            <th style="text-align:center">✓</th>
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  <li><a data-toggle="pill" href="#Partcollection">Part Collection</a></li>
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                <td>Wiki page to document our project</td>
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</ul>
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            </tr>
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        <tr>
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            <th style="text-align:center">✓</th>
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                <td>Project Attribution (Wiki page/About/Attributions)</td>
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            </tr>
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        <tr>
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            <th style="text-align:center">✓</th>
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                <td>Safety Forms submission (Safety Check-in not required as all materials and parts that we are working with are on the White List)</td>
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                </tr>
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        <tr>
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            <th style="text-align:center">✓</th>
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                <td>Judging Form submission</td>
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            </tr>
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        <tr>
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            <th style="text-align:center">✓</th>
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                <td>New Part submitted to the Registry and documented on the Wiki page (Wiki page/Achievements/Parts):BBa_K 2054000-BBa_K20540006 [Received, Accepted]</td>
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            </tr> 
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        <tr>
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            <th style="text-align:center" colspan="2">For Silver Medal</th>
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            </tr>
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        <tr>
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            <th style="text-align:center">✓</th>
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                <td>Submit a new part to the iGEM Parts Registry and document its characterization in the Main Page section of that corresponding Part Registry entry: BBa_KBBa_K2054001</td>
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            </tr>
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        <tr>
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            <th style="text-align:center">✓</th>
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                <td>Experimentally validated the detection function of our tetrahedron nanostructure assembled from Parts:
 +
                BBa_K2054001, BBa_K2054002, BBa_K2054003, BBa_K2054004 and BBa_K2054005,
 +
                constructs produced signals in ABTS assays as expected</td>
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            </tr>
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        <tr>
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            <th style="text-align:center">✓</th>
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                <td>Mentoring the new high school iGEM Team (Team Hong_Kong_UCCKE)</td>
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            </tr>
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        <tr>
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            <th style="text-align:center">✓</th>
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                <td>Public Engagement Human Practices: Public exhibition at JSSE, and sharing iGEM experience with local high school students (at UCCKE campus)</td>
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            </tr>
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            <tr>
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            <th style="text-align:center" colspan="2">For Gold Medal</th>
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            </tr>
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        <tr>
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            <th style="text-align:center">✓</th>
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                <td>Inspired from an integrated Human Practice<br>
 +
                The concept DNA nanostructure assembly and miRNA detection were integrated into our public exhibition at The 49th Joint School Science Exhibition.
 +
                Feedbacks from visitors offered us great inspiration the future application of our constructs and the potential social impacts of our project.
 +
                The creation of the Facebook video  "Biosafety and Research Ethics- Conversation with Profession Danny Chan" inspired us to conduct a thorough risk assessment on E. coli DH10beta, which is a crucial component of our project.</td>
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            </tr>
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        <tr>
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            <th style="text-align:center">✓</th>
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                <td>Demostrate the proof of concept<br>We used our nanostructure to test on the RNA inputs and mutants and our nanostructure worked as expected. </td>
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            </tr>
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            <tr>
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            <th style="text-align:center">✓</th>
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                <td>Demonstrate<br>Our nanostructure passed tests on RNA inputs to fulfill real life condition. </td>
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            </tr>
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Latest revision as of 02:59, 20 October 2016

Achievements


We spent the entire Summer in the lab, and such hard work resulted in some achievements. We have submitted parts to the registry, experimentally validated our new BioBrick devices and demonstrated the assembly of our design and fulfilled the criteria for a gold medal.

Parts


Featured Parts
BBa_K2054000 - ssDNAs of our nanostructure
Forwarding our all submitted parts, we agree that it would be better if there is mighty single biobrick that encodes the all components of our desired structure. And here we have it – putting all together .
BBa_K2054006 - ssDNAs of our simplified nanostructure
Oligos 1 and 5 are in a single plasmid to encode for the simplified version of our nanostructure.

Table of Parts

Type Name (Part number) Type Description Length(bp)
Composite Part
(Featured)		 BBa_K2054000 DNA ALL oligos included in our desired nanostructure 3561
Basic Part BBa_K2054001 DNA Oligo 1 for the Tetrahedral nanostructure, w/ split G-quadruplex 2388
Basic Part BBa_K2054002 DNA Oligo 2 for the Tetrahedral nanostructure 2358
Basic Part BBa_K2054003 DNA Oligo 3 of the Tetrahedral nanostructure 2375
Basic Part BBa_K2054004 DNA Oligo 4 of the Tetrahedral nanostructure 2375
Basic Part BBa_K2054005 DNA Oligo 5 of the Tetrahedral nanostructure 2313
Composite Part
(Featured)		 BBa_K2054006 DNA Oligos of the simplified 2D nanostructure 2639

Achievements

For Bronze Medal
✓ Team Registration and Giant Jamboree Attendance (where we have prepared a 20-minutes presentation and a poster)
✓ Wiki page to document our project
✓ Project Attribution (Wiki page/About/Attributions)
✓ Safety Forms submission (Safety Check-in not required as all materials and parts that we are working with are on the White List)
✓ Judging Form submission
✓ New Part submitted to the Registry and documented on the Wiki page (Wiki page/Achievements/Parts):BBa_K 2054000-BBa_K20540006 [Received, Accepted]
For Silver Medal
✓ Submit a new part to the iGEM Parts Registry and document its characterization in the Main Page section of that corresponding Part Registry entry: BBa_KBBa_K2054001
✓ Experimentally validated the detection function of our tetrahedron nanostructure assembled from Parts: BBa_K2054001, BBa_K2054002, BBa_K2054003, BBa_K2054004 and BBa_K2054005, constructs produced signals in ABTS assays as expected
✓ Mentoring the new high school iGEM Team (Team Hong_Kong_UCCKE)
✓ Public Engagement Human Practices: Public exhibition at JSSE, and sharing iGEM experience with local high school students (at UCCKE campus)
For Gold Medal
✓ Inspired from an integrated Human Practice
The concept DNA nanostructure assembly and miRNA detection were integrated into our public exhibition at The 49th Joint School Science Exhibition. Feedbacks from visitors offered us great inspiration the future application of our constructs and the potential social impacts of our project. The creation of the Facebook video "Biosafety and Research Ethics- Conversation with Profession Danny Chan" inspired us to conduct a thorough risk assessment on E. coli DH10beta, which is a crucial component of our project.
✓ Demostrate the proof of concept
We used our nanostructure to test on the RNA inputs and mutants and our nanostructure worked as expected.
✓ Demonstrate
Our nanostructure passed tests on RNA inputs to fulfill real life condition.


Copyright © 2016 HKU iGEM. All rights reserved.