Difference between revisions of "Team:Hong Kong HKU/Parts"

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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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<h5>Note</h5>
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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<h5>Adding parts to the registry</h5>
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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<h5>What information do I need to start putting my parts on the Registry?</h5>
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<p>The information needed to initially create a part on the Registry is:</p>
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<ul>
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<li>Part Name</li>
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<li>Part type</li>
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<li>Creator</li>
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<li>Sequence</li>
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<li>Short Description (60 characters on what the DNA does)</li>
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<li>Long Description (Longer description of what the DNA does)</li>
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<li>Design considerations</li>
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</ul>
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<p>
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
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<h5>Inspiration</h5>
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
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<ul>
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
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<h5>Part Table </h5>
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<groupparts>iGEM2016 Example</groupparts>
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{{Hong_Kong_HKU/Header}}
 
<html>
 
<html>
 +
<body>
 +
<div class="container" align="center">
 +
    <h3>Achievements</h3><br>
 +
    <ul class="nav nav-pills">
 +
      <li class="active"><a data-toggle="pill" href="#Parts">Parts</a></li>
 +
      <li><a data-toggle="pill" href="#Achievements">Achievements</a></li>
 +
      <li><a href="https://2016.igem.org/Team:Hong_Kong_HKU/Results">Results</a></li>
 +
      <li><a href="https://2016.igem.org/Team:Hong_Kong_HKU/Demonstrate">Demonstrate our work</a></li>
 +
      <li><a href="https://2016.igem.org/Team:Hong_Kong_HKU/Proof">Proof of Concept</a></li>
 +
    </ul>
 +
    <p class="text-justify" align="left"><font size="3">
 +
    We spent the entire Summer in the lab, and such hard work resulted in some achievements.
 +
    We have submitted parts to the registry, experimentally validated our new BioBrick devices and demonstrated the assembly of our design and fulfilled the criteria for a gold medal.
 +
    </font></p>
 +
    <div class="tab-content">
 +
    <div id="Parts" class="tab-pane fade in active">
 +
        <h3>Parts</h3><hr><br>
 +
<div class="panel-group" id="PartsContent" role="tablist" aria-multiselectable="true">
 +
        <div class="panel panel-transparent">
 +
                <div class="panel-heading" role="tab">
 +
                  <h4 class="panel-title"><a data-toggle="collapse" data-parent="#PartsContent" href="#Part1"><h4>Biobricks</h4></a></h4>
 +
                </div>
 +
                <div id="Part1" class="panel-collapse collapse in">
 +
                  <div class="panel-body">
 +
                  <p class="text-justify" align="left">
 +
                  <font size="4"><b>Featured Parts</b></font><br>
 +
                  <font size="3">
 +
                  <b><a href="http://parts.igem.org/Part:BBa_K2054000">BBa_K2054000</a> - ssDNAs of our nanostructure</b><br>
 +
                  Forwarding our all submitted parts, we agree that it would be better if there is mighty single biobrick that encodes the all components of our desired structure.
 +
                  And here we have it – putting all together . <br>
 +
                  <b><a href="http://parts.igem.org/Part:BBa_K2054006">BBa_K2054006</a> - ssDNAs of our simplified nanostructure</b><br>
 +
                  Oligos 1 and 5 are in a single plasmid to encode for the simplified version of our nanostructure.<br><br>
 +
                  <font size="4"><b>Table of Parts</b></font><br>
 +
                  </font></p>
 +
                    <table class="table">
 +
                        <thead>
 +
                            <tr>
 +
                                <th style="text-align:center">Type</th>
 +
                                <th style="text-align:center">Name (Part number)</th>
 +
                                <th style="text-align:center">Type</th>
 +
                                <th style="text-align:center">Description</th>
 +
                                <th style="text-align:center">Length(bp)</th>
 +
                            </tr>
 +
                        </thead>
 +
                        <tbody>
 +
                            <tr>
 +
                                <td style="text-align:center">Composite Part<br>(Featured)</td>
 +
                                <td style="text-align:center"><a href="http://parts.igem.org/Part:BBa_K2054000">BBa_K2054000</a></td>
 +
                                <td style="text-align:center">DNA</td>
 +
                                <td style="text-align:center">ALL oligos included in our desired nanostructure</td>
 +
                                <td style="text-align:center">3561</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td style="text-align:center">Basic Part</td>
 +
                                <td style="text-align:center"><a href="http://parts.igem.org/Part:BBa_K2054001">BBa_K2054001</a></td>
 +
                                <td style="text-align:center">DNA</td>
 +
                                <td style="text-align:center">Oligo 1 for the Tetrahedral nanostructure, w/ split G-quadruplex</td>
 +
                                <td style="text-align:center">2388</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td style="text-align:center">Basic Part</td>
 +
                                <td style="text-align:center"><a href="http://parts.igem.org/Part:BBa_K2054002">BBa_K2054002</a></td>
 +
                                <td style="text-align:center">DNA</td>
 +
                                <td style="text-align:center">Oligo 2 for the Tetrahedral nanostructure</td>
 +
                                <td style="text-align:center">2358</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td style="text-align:center">Basic Part</td>
 +
                                <td style="text-align:center"><a href="http://parts.igem.org/Part:BBa_K2054003">BBa_K2054003</a></td>
 +
                                <td style="text-align:center">DNA</td>
 +
                                <td style="text-align:center">Oligo 3 of the Tetrahedral nanostructure</td>
 +
                                <td style="text-align:center">2375</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td style="text-align:center">Basic Part</td>
 +
                                <td style="text-align:center"><a href="http://parts.igem.org/Part:BBa_K2054004">BBa_K2054004</a></td>
 +
                                <td style="text-align:center">DNA</td>
 +
                                <td style="text-align:center">Oligo 4 of the Tetrahedral nanostructure</td>
 +
                                <td style="text-align:center">2375</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td style="text-align:center">Basic Part</td>
 +
                                <td style="text-align:center"><a href="http://parts.igem.org/Part:BBa_K2054005">BBa_K2054005</a></td>
 +
                                <td style="text-align:center">DNA</td>
 +
                                <td style="text-align:center">Oligo 5 of the Tetrahedral nanostructure</td>
 +
                                <td style="text-align:center">2313</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td style="text-align:center">Composite Part<br>(Featured)</td>
 +
                                <td style="text-align:center"><a href="http://parts.igem.org/Part:BBa_K2054006">BBa_K2054006</a></td>
 +
                                <td style="text-align:center">DNA</td>
 +
                                <td style="text-align:center">Oligos of the simplified 2D nanostructure </td>
 +
                                <td style="text-align:center">2639</td>
 +
                            </tr>
 +
                        </tbody>
 +
                    </table>
 +
                </div>
 +
            </div>
 +
          </div>
 +
        </div>
 +
    </div>
 +
    <div id="Achievements" class="tab-pane fade">
 +
    <h3>Achievements</h3>
 +
    <p class="text-justify" align="left"><font size="3">
 +
    <table class="table">
 +
    <thead>
 +
        <tr>
 +
            <th style="text-align:center" colspan="2">For Bronze Medal</th>
 +
            </tr>
 +
        </thead>
 +
        <tbody>
 +
        <tr>
 +
            <th style="text-align:center">✓</th>
 +
                <td>Team Registration and Giant Jamboree Attendance (where we have prepared a 20-minutes presentation and a poster)</td>
 +
            </tr>
 +
        <tr>
 +
            <th style="text-align:center">✓</th>
 +
                <td>Wiki page to document our project</td>
 +
            </tr>
 +
        <tr>
 +
            <th style="text-align:center">✓</th>
 +
                <td>Project Attribution (Wiki page/About/Attributions)</td>
 +
            </tr>
 +
        <tr>
 +
            <th style="text-align:center">✓</th>
 +
                <td>Safety Forms submission (Safety Check-in not required as all materials and parts that we are working with are on the White List)</td>
 +
                </tr>
 +
        <tr>
 +
            <th style="text-align:center">✓</th>
 +
                <td>Judging Form submission</td>
 +
            </tr>
 +
        <tr>
 +
            <th style="text-align:center">✓</th>
 +
                <td>New Part submitted to the Registry and documented on the Wiki page (Wiki page/Achievements/Parts):BBa_K 2054000-BBa_K20540006 [Received, Accepted]</td>
 +
            </tr> 
 +
        <tr>
 +
            <th style="text-align:center" colspan="2">For Silver Medal</th>
 +
            </tr>
 +
        <tr>
 +
            <th style="text-align:center">✓</th>
 +
                <td>Submit a new part to the iGEM Parts Registry and document its characterization in the Main Page section of that corresponding Part Registry entry: BBa_KBBa_K2054001</td>
 +
            </tr>
 +
        <tr>
 +
            <th style="text-align:center">✓</th>
 +
                <td>Experimentally validated the detection function of our tetrahedron nanostructure assembled from Parts:
 +
                BBa_K2054001, BBa_K2054002, BBa_K2054003, BBa_K2054004 and BBa_K2054005,
 +
                constructs produced signals in ABTS assays as expected</td>
 +
            </tr>
 +
        <tr>
 +
            <th style="text-align:center">✓</th>
 +
                <td>Mentoring the new high school iGEM Team (Team Hong_Kong_UCCKE)</td>
 +
            </tr>
 +
        <tr>
 +
            <th style="text-align:center">✓</th>
 +
                <td>Public Engagement Human Practices: Public exhibition at JSSE, and sharing iGEM experience with local high school students (at UCCKE campus)</td>
 +
            </tr>
 +
            <tr>
 +
            <th style="text-align:center" colspan="2">For Gold Medal</th>
 +
            </tr>
 +
        <tr>
 +
            <th style="text-align:center">✓</th>
 +
                <td>Inspired from an integrated Human Practice<br>
 +
                The concept DNA nanostructure assembly and miRNA detection were integrated into our public exhibition at The 49th Joint School Science Exhibition.
 +
                Feedbacks from visitors offered us great inspiration the future application of our constructs and the potential social impacts of our project.
 +
                The creation of the Facebook video  "Biosafety and Research Ethics- Conversation with Profession Danny Chan" inspired us to conduct a thorough risk assessment on E. coli DH10beta, which is a crucial component of our project.</td>
 +
            </tr>
 +
        <tr>
 +
            <th style="text-align:center">✓</th>
 +
                <td>Demostrate the proof of concept<br>We used our nanostructure to test on the RNA inputs and mutants and our nanostructure worked as expected. </td>
 +
            </tr>
 +
            <tr>
 +
            <th style="text-align:center">✓</th>
 +
                <td>Demonstrate<br>Our nanostructure passed tests on RNA inputs to fulfill real life condition. </td>
 +
            </tr>
 +
        </tbody>
 +
    </table>
 +
    </font></p>
 +
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{{Hong_Kong_HKU/Footer}}

Latest revision as of 02:59, 20 October 2016

Achievements


We spent the entire Summer in the lab, and such hard work resulted in some achievements. We have submitted parts to the registry, experimentally validated our new BioBrick devices and demonstrated the assembly of our design and fulfilled the criteria for a gold medal.

Parts


Featured Parts
BBa_K2054000 - ssDNAs of our nanostructure
Forwarding our all submitted parts, we agree that it would be better if there is mighty single biobrick that encodes the all components of our desired structure. And here we have it – putting all together .
BBa_K2054006 - ssDNAs of our simplified nanostructure
Oligos 1 and 5 are in a single plasmid to encode for the simplified version of our nanostructure.

Table of Parts

Type Name (Part number) Type Description Length(bp)
Composite Part
(Featured)		 BBa_K2054000 DNA ALL oligos included in our desired nanostructure 3561
Basic Part BBa_K2054001 DNA Oligo 1 for the Tetrahedral nanostructure, w/ split G-quadruplex 2388
Basic Part BBa_K2054002 DNA Oligo 2 for the Tetrahedral nanostructure 2358
Basic Part BBa_K2054003 DNA Oligo 3 of the Tetrahedral nanostructure 2375
Basic Part BBa_K2054004 DNA Oligo 4 of the Tetrahedral nanostructure 2375
Basic Part BBa_K2054005 DNA Oligo 5 of the Tetrahedral nanostructure 2313
Composite Part
(Featured)		 BBa_K2054006 DNA Oligos of the simplified 2D nanostructure 2639

Achievements

For Bronze Medal
✓ Team Registration and Giant Jamboree Attendance (where we have prepared a 20-minutes presentation and a poster)
✓ Wiki page to document our project
✓ Project Attribution (Wiki page/About/Attributions)
✓ Safety Forms submission (Safety Check-in not required as all materials and parts that we are working with are on the White List)
✓ Judging Form submission
✓ New Part submitted to the Registry and documented on the Wiki page (Wiki page/Achievements/Parts):BBa_K 2054000-BBa_K20540006 [Received, Accepted]
For Silver Medal
✓ Submit a new part to the iGEM Parts Registry and document its characterization in the Main Page section of that corresponding Part Registry entry: BBa_KBBa_K2054001
✓ Experimentally validated the detection function of our tetrahedron nanostructure assembled from Parts: BBa_K2054001, BBa_K2054002, BBa_K2054003, BBa_K2054004 and BBa_K2054005, constructs produced signals in ABTS assays as expected
✓ Mentoring the new high school iGEM Team (Team Hong_Kong_UCCKE)
✓ Public Engagement Human Practices: Public exhibition at JSSE, and sharing iGEM experience with local high school students (at UCCKE campus)
For Gold Medal
✓ Inspired from an integrated Human Practice
The concept DNA nanostructure assembly and miRNA detection were integrated into our public exhibition at The 49th Joint School Science Exhibition. Feedbacks from visitors offered us great inspiration the future application of our constructs and the potential social impacts of our project. The creation of the Facebook video "Biosafety and Research Ethics- Conversation with Profession Danny Chan" inspired us to conduct a thorough risk assessment on E. coli DH10beta, which is a crucial component of our project.
✓ Demostrate the proof of concept
We used our nanostructure to test on the RNA inputs and mutants and our nanostructure worked as expected.
✓ Demonstrate
Our nanostructure passed tests on RNA inputs to fulfill real life condition.


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