Difference between revisions of "Team:Nagahama"

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{{Nagahama}}
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'''{{Nagahama}}'''
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'''長浜'''
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=Increased production of fragrance for new food preservation method ''E.coli''=
  
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==ABSTRACT==
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<br />
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To innovate the refrigerator without electricity, we invented a prototype of FRAVORATOR that is possible to preserve food by antibacterial volatiles synthesized by ''Escherichia coli''. For the high-efficiency synthetic process of preservation flavor, farnesol, we focused on improving its synthetic pathway. First, 1-deoxy-D-xylulose 5-phosphate（DXP）synthase, Dxs, is down-regulated when its downstream material has been produced to some extent. So, we tried to over-express, another DXP synthase, nDXP, that rarely has been used in normal conditions. Also, we created fusion gene for ispC-ribB protein for enzymatic function. Second, to avoid recombinant in the real usage, we tried to knock out synthase that produces unnecessary byproduct by genome editing.
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Project description
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<br />
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<br />
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To innovate a refrigerator without electricity, we invented a prototype of FRAVORATOR that is possible to preserve food by antibacterial volatiles synthesized by Escherichia coli.
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<br />
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<br />
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For high-efficiency synthetic processs, we tried two methods. 1, 1-deoxy-D-xylulose 5-phosphate（DXP）synthase, Dxs, is down-regulated when its downstream material has been produced to some extent. So, we tried to over-express, another DXP synthase, nDXP, that rarely has been used in normal conditions. 2, Using genomic editing method, we tried to knock out synthase, that we<br /> used to produce unnecessary by product.
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==TRACK==
 
==TRACK==
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|2
 
|2
 
|Meet all deliverables on the Requirements page (section 3).  
 
|Meet all deliverables on the Requirements page (section 3).  
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|Please check [https://igem.org/2015_Judging_Form?id=1653 Here].
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|We meet all requirements.
 
|-
 
|-
 
|3
 
|3
 
|Create a page on your team wiki with clear attribution of each aspect of your project. This page must clearly attribute work done by the students and distinguish it from work done by others, including host labs, advisors, instructors, sponsors, professional website designers, artists, and commercial services.
 
|Create a page on your team wiki with clear attribution of each aspect of your project. This page must clearly attribute work done by the students and distinguish it from work done by others, including host labs, advisors, instructors, sponsors, professional website designers, artists, and commercial services.
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|style="white-space:nowrap" |Please check [https://2016.igem.org/Team:Nagahama/Attributions Here].
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|Please check [https://2016.igem.org/Team:Nagahama/Attributions Here].
 
|-
 
|-
 
|4
 
|4
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|Present a poster and a talk at the iGEM Jamboree.
 
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|Yes. We are looking forward to participating in the iGEM Jamboree!!
 
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|-
 
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|5
 
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|Create a page on your team wiki with clear attribution of each aspect of your project. This page must clearly attribute work done by the students and distinguish it from work done by others, including host labs, advisors, instructors, sponsors, professional website designers, artists, and commercial services.
 
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|[https://2015.igem.org/Team:Nagahama/Attributions Here] is our attribution page.
 
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|-
 
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|6
 
 
|Document at least one new standard BioBrick Part or Device central to your project and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines). You may also document a new application of a BioBrick part from a previous iGEM year, adding that documentation to the part's main page.
 
|Document at least one new standard BioBrick Part or Device central to your project and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines). You may also document a new application of a BioBrick part from a previous iGEM year, adding that documentation to the part's main page.
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|Yes, [https://2015.igem.org/Team:Nagahama/Parts here] is our BioBrick parts collection.
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|Please check [https://2016.igem.org/Team:Nagahama/Our_all_BioBricks Here].
 
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|-
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|}
 
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|-
 
|1
 
|1
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|Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected. Document the characterization of this part in the Main Page section of the Registry entry for that Part/Device. This working part must be different from the part you documented in Bronze medal criterion
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|Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected. Document the characterization of this part in the Main Page section of the Registry entry for that Part/Device. This working part must be different from the part you documented in Bronze medal criterion #4. Submit this part to the iGEM Parts Registry.
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|We created 3 new BioBrick devices for silver medal criterion. Please look at [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1653025 BBa_K1653025] , [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1653024 BBa_K1653024], and [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1653027 BBa_K1653027].
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|We created 3 new BioBrick devices for silver medal criterion. Please look at [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1950010 BBa_K1950010] , [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1950008 BBa_K1950008], and [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1950007 BBa_K1950007].
 
|-
 
|-
 
|2
 
|2
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|Submit this new part to the iGEM Parts Registry. This part must be different from the part you documented in Bronze medal criterion
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|Convince the judges you have helped any registered iGEM team from a high-school, different track, another university, or institution in a significant way by, for example, mentoring a new team, characterizing a part, debugging a construct, modeling/simulating their system or helping validate a software/hardware solution to a synthetic biology problem.
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|We created 3 new BioBrick devices for silver medal criterion. And then we have submit these parts to iGEM Parts Registry. Please look at [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1653025 BBa_K1653025] , [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1653024 BBa_K1653024], and [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1653027 BBa_K1653027].
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|Please check [https://2016.igem.org/Team:Nagahama/Collaborations here].
 
|-
 
|-
 
|3
 
|3
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|iGEM projects involve important questions beyond the bench, for example relating to (but not limited to) ethics, sustainability, social justice, safety, security, and intellectual property rights. We refer to these activities as Human Practices in iGEM. Demonstrate how your team has identified, investigated and addressed one or more of these issues in the context of your project.
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|iGEM projects involve important questions beyond the lab bench, for example relating to (but not limited to) ethics, sustainability, social justice, safety, security, and intellectual property rights. Demonstrate how your team has identified, investigated, and addressed one or more of these issues in the context of your project. Your activity could center around education, public engagement, public policy issues, public perception, or other activities (see the human practices hub for more information and examples of previous teams' exemplary work).
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|Our important question is Risk to commercialize the “Flavorator” beyond the bench. We have addressed [https://2015.igem.org/Team:Nagahama/Issues Risk Assessment].
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|Please check [https://2016.igem.org/Team:Nagahama/Human_Practice here].
 
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|Choose one of these two options: (1) Expand on your silver medal Human Practices activity by demonstrating how you have integrated the investigated issues into the design and/or execution of your project. OR (2) Demonstrate an innovative Human Practices activity that relates to your project (this typically involves educational, public engagement, and/or public perception activities
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|Expand on your silver medal activity by demonstrating how you have integrated the investigated issues into the design and/or execution of your project.
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|We have expanded our Risk Assessment for silver medal criterion. [https://2015.igem.org/Team:Nagahama/Issues]. And we have addresses innovative HP.[https://2015.igem.org/Team:Nagahama/Innovation_HP]
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|Please check [https://2016.igem.org/Team:Nagahama/Intgrated_Human_Practice here].
 
|-
 
|-
 
|2
 
|2
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|Help any registered iGEM team from a high-school, different track, another university, or institution in a significant way by, for example, mentoring a new team, characterizing a part, debugging a construct, modeling/simulating their system or helping validate a software/hardware solution to a synbio problem.
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|Improve the function OR characterization of an existing BioBrick Part or Device and enter this information in the Registry. Please see the Registry help page on how to document a contribution to an existing part. This part must NOT be from your 2016 part number range.  
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|We helped Tokyo_Tech to do chemotaxis experiment.[https://2015.igem.org/Team:Nagahama/awarming]. In addition, we helped Uniandes_Colombia HP "LOW BUDGET".[https://2015.igem.org/Team:Nagahama/colombia]
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|Please check [http://parts.igem.org/Part:BBa_K1230000 here].
 
|-
 
|-
 
|3
 
|3
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|Improve the function OR characterization of a previously existing BioBrick Part or Device (created by another team, or by your own team in in a previous year of iGEM), and enter this information in the part's page on the Registry. Please see the Registry Contribution help page for help on documenting a contribution to an existing part. This part must not come from your team's 2015 range of part numbers.
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|Demonstrate a functional proof of concept of your project. Your proof of concept must consist of a BioBrick device; a single BioBrick part cannot constitute a proof of concept. (Remember, biological materials may not be taken outside the lab.)
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|We improved the characterization of a previously existing BioBrick Part [http://parts.igem.org/Part:BBa_K1230000 BBa_K1230000].
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|Please check [https://2016.igem.org/Team:Nagahama/Proof_of_concept here].
 
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|-
 
|4
 
|4
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|Demonstrate a functional prototype of your project. Your prototype can derive from a previous project (that was not demonstrated to work) by your team or by another team. Show this system working under real-world conditions that you simulate in the lab.  
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|Show your project working under real-world conditions. To achieve this criterion, you should demonstrate your whole system, or a functional proof of concept working under simulated conditions in the lab (Remember, biological materials may not be taken outside the lab.) 
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|We Demonstrate a functional prototype of "Flavorator".　[https://2015.igem.org/Team:Nagahama/Entrepreneurship]
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|Please check [https://2016.igem.org/Team:Nagahama/Demonstrate_your_work here].
 
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<div class="column full_size" >
 
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<h2> Welcome to iGEM 2016! </h2>
 
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
 
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</div>
 
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<h5>Before you start: </h5>
 
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<p> Please read the following pages:</p>
 
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<ul>
 
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<li>  <a href="https://2016.igem.org/Requirements">Requirements page </a> </li>
 
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<li> <a href="https://2016.igem.org/Wiki_How-To">Wiki Requirements page</a></li>
 
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<li> <a href="https://2016.igem.org/Resources/Template_Documentation"> Template Documentation </a></li>
 
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</ul>
 
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<div class="highlight">
 
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<h5> Styling your wiki </h5>
 
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
 
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
 
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<h5> Wiki template information </h5>
 
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
 
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<h5> Editing your wiki </h5>
 
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
 
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<p> <a href="https://2016.igem.org/wiki/index.php?title=Team:Example&action=edit"> Click here to edit this page! </a></p>
 
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<h5>Tips</h5>
 
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
 
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<ul>
 
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
 
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<li>Be clear about what you are doing and how you plan to do this.</li>
 
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
 
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
 
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
 
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2016.igem.org/Calendar">iGEM 2016 calendar</a> </li>
 
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<li>Have lots of fun! </li>
 
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</ul>
 
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<h5>Inspiration</h5>
 
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
 
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<ul>
 
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
 
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
 
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
 
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
 
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
 
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
 
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</ul>
 
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<h5> Uploading pictures and files </h5>
 
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<p> You can upload your pictures and files to the iGEM 2016 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
 
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When you upload, set the "Destination Filename" to <code>Team:YourOfficialTeamName/NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
 
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<div class="button_click"  onClick=" parent.location= 'https://2016.igem.org/Special:Upload '"> 
 
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UPLOAD FILES
 
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Latest revision as of 03:01, 20 October 2016

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Increased production of fragrance for new food preservation method E.coli

ABSTRACT

To innovate the refrigerator without electricity, we invented a prototype of FRAVORATOR that is possible to preserve food by antibacterial volatiles synthesized by Escherichia coli. For the high-efficiency synthetic process of preservation flavor, farnesol, we focused on improving its synthetic pathway. First, 1-deoxy-D-xylulose 5-phosphate（DXP）synthase, Dxs, is down-regulated when its downstream material has been produced to some extent. So, we tried to over-express, another DXP synthase, nDXP, that rarely has been used in normal conditions. Also, we created fusion gene for ispC-ribB protein for enzymatic function. Second, to avoid recombinant in the real usage, we tried to knock out synthase that produces unnecessary byproduct by genome editing.

TRACK

Food Nutrition

We certainly achieved GOLD Medal Criteria!

Bronze Medal

1 Register for iGEM, have a great summer, and attend the Giant Jamboree. Yes, we are looking forward to taking part in the Giant Jamboree.
2 Meet all deliverables on the Requirements page (section 3). We meet all requirements.
3 Create a page on your team wiki with clear attribution of each aspect of your project. This page must clearly attribute work done by the students and distinguish it from work done by others, including host labs, advisors, instructors, sponsors, professional website designers, artists, and commercial services. Please check Here.
4 Document at least one new standard BioBrick Part or Device central to your project and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines). You may also document a new application of a BioBrick part from a previous iGEM year, adding that documentation to the part's main page. Please check Here.

Silver Medal

1 Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected. Document the characterization of this part in the Main Page section of the Registry entry for that Part/Device. This working part must be different from the part you documented in Bronze medal criterion #4. Submit this part to the iGEM Parts Registry. We created 3 new BioBrick devices for silver medal criterion. Please look at [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1950010 BBa_K1950010] , [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1950008 BBa_K1950008], and [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1950007 BBa_K1950007].
2 Convince the judges you have helped any registered iGEM team from a high-school, different track, another university, or institution in a significant way by, for example, mentoring a new team, characterizing a part, debugging a construct, modeling/simulating their system or helping validate a software/hardware solution to a synthetic biology problem. Please check here.
3 iGEM projects involve important questions beyond the lab bench, for example relating to (but not limited to) ethics, sustainability, social justice, safety, security, and intellectual property rights. Demonstrate how your team has identified, investigated, and addressed one or more of these issues in the context of your project. Your activity could center around education, public engagement, public policy issues, public perception, or other activities (see the human practices hub for more information and examples of previous teams' exemplary work). Please check here.

Gold Medal

1 Expand on your silver medal activity by demonstrating how you have integrated the investigated issues into the design and/or execution of your project. Please check here.
2 Improve the function OR characterization of an existing BioBrick Part or Device and enter this information in the Registry. Please see the Registry help page on how to document a contribution to an existing part. This part must NOT be from your 2016 part number range. Please check [http://parts.igem.org/Part:BBa_K1230000 here].
3 Demonstrate a functional proof of concept of your project. Your proof of concept must consist of a BioBrick device; a single BioBrick part cannot constitute a proof of concept. (Remember, biological materials may not be taken outside the lab.) Please check here.
4 Show your project working under real-world conditions. To achieve this criterion, you should demonstrate your whole system, or a functional proof of concept working under simulated conditions in the lab (Remember, biological materials may not be taken outside the lab.) Please check here.