Difference between revisions of "Team:KAIT Japan/Description"

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{{KAIT_Japan}}
 
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<h1>"Soil conservation by Bacteria Cellulose"</h1>
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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the<a href="https://2016.igem.org/Judging/Medals"> improve a previous part or project gold medal criterion</a>. </p>
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<p> Delete this box in order to be evaluated for this medal. See more information at <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions"> Instructions for Pages for awards</a>.</p>
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<p>Tell us about your project, describe what moves you and why this is something important for your team.</p>
 
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<h5>What should this page contain?</h5>
 
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<li> A clear and concise description of your project.</li>
 
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<li>A detailed explanation of why your team chose to work on this particular project.</li>
 
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<li>References and sources to document your research.</li>
 
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<li>Use illustrations and other visual resources to explain your project.</li>
 
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<h5>Advice on writing your Project Description</h5>
 
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We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be consist, accurate and unambiguous in your achievements.
 
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Judges like to read your wiki and know exactly what you have achieved. This is how you should think about these sections; from the point of view of the judge evaluating you at the end of the year.
 
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<h5>References</h5>
 
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<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.</p>
 
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<h5>Inspiration</h5>
 
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<p>See how other teams have described and presented their projects: </p>
 
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<li><a href="https://2014.igem.org/Team:Imperial/Project"> Imperial</a></li>
 
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<li><a href="https://2014.igem.org/Team:UC_Davis/Project_Overview"> UC Davis</a></li>
 
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<li><a href="https://2014.igem.org/Team:SYSU-Software/Overview">SYSU Software</a></li>
 
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<h3>Description</h3>
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Project
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-Soil Conservation by Bacteria Cellulose-
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A. xylinum is known as a type of bacteria which produces Bacteria Cellulose(BC).
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Acetobacter intake glucose as sources of nutrient and produces Bacteria Cellulose by Cellulose Synthesis Pathway of Acetobacter.
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<img src="https://static.igem.org/mediawiki/2016/5/5a/T--KAIT_Japan--図１.jpg">
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Synthesis Pathway of Acetobacter
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The pathway above is not only used to produce cellulose, but also used for other functions by glucose. Which causes the production of cellulose to be not so effective. So, we planned to diminish the amount of the enzymes G6PD and PGI. And to diminish the amount of the enzymes G6PD and PGI, we are using the method of antisense which will lead to deactivate of both the enzymes G6PD and PGI.
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<br>
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When antisense genes are expressed, an mRNA molecule is produced that is a mirror image of the targeted gene. The two, opposite mRNAs bind to one another, disrupting their function and making protein synthesis impossible. In effect, the targeted gene is blocked and transcription is prevented. Therefore, glucose will efficiently uses this pathway to produce cellulose which leads to increment of Bacteria Cellulose. And we think that, by using this modified gene, we can protect our environment as we can guarantee the humidity of soil to prevent desertification and soil erosion.
  
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-Suicide System-
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We design this system with the goal to limit the prevalence of genetically modified organisms in nature as this genetically modified organism system is designed to scatter in nature.
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<img src="https://static.igem.org/mediawiki/2016/c/cc/T--KAIT_Japan--plac.png">
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Suicide system designed
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This system works when lactose is present, as placI works when lactose is present. And when placI works, it produce repressor that will binds with pλ. Therefore, K592024, I14044 and B0015 cannot be transcript. But, when lactose is absent, placI will be malfunctioned which leads to transcription failure of C0051 and B0015. And, pλwill works and transcript K592024, I14044, B0015. lacI will be produced and binds with placI.
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<br>
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However, as K592024 is a reporter gene, if it is not being reform, it cannot works as a suicide system. To let K592024 works as a suicide system, K592024 is changed to ccdB gene. If ccdB gene is being expressed, it proves that CcdB protein is being produced. CcdB protein works to inhibit DNA gyrase. That will leads to the commit suicide of bacteria.
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<img src="https://2016.igem.org/File:T--KAIT_Japan--Partsparts.png">
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Latest revision as of 03:16, 20 October 2016

"Soil conservation by Bacteria Cellulose"

Description

Project
-Soil Conservation by Bacteria Cellulose-
A. xylinum is known as a type of bacteria which produces Bacteria Cellulose(BC).
Acetobacter intake glucose as sources of nutrient and produces Bacteria Cellulose by Cellulose Synthesis Pathway of Acetobacter.

Synthesis Pathway of Acetobacter
The pathway above is not only used to produce cellulose, but also used for other functions by glucose. Which causes the production of cellulose to be not so effective. So, we planned to diminish the amount of the enzymes G6PD and PGI. And to diminish the amount of the enzymes G6PD and PGI, we are using the method of antisense which will lead to deactivate of both the enzymes G6PD and PGI.
When antisense genes are expressed, an mRNA molecule is produced that is a mirror image of the targeted gene. The two, opposite mRNAs bind to one another, disrupting their function and making protein synthesis impossible. In effect, the targeted gene is blocked and transcription is prevented. Therefore, glucose will efficiently uses this pathway to produce cellulose which leads to increment of Bacteria Cellulose. And we think that, by using this modified gene, we can protect our environment as we can guarantee the humidity of soil to prevent desertification and soil erosion.

-Suicide System-
We design this system with the goal to limit the prevalence of genetically modified organisms in nature as this genetically modified organism system is designed to scatter in nature.

Suicide system designed
This system works when lactose is present, as placI works when lactose is present. And when placI works, it produce repressor that will binds with pλ. Therefore, K592024, I14044 and B0015 cannot be transcript. But, when lactose is absent, placI will be malfunctioned which leads to transcription failure of C0051 and B0015. And, pλwill works and transcript K592024, I14044, B0015. lacI will be produced and binds with placI.
However, as K592024 is a reporter gene, if it is not being reform, it cannot works as a suicide system. To let K592024 works as a suicide system, K592024 is changed to ccdB gene. If ccdB gene is being expressed, it proves that CcdB protein is being produced. CcdB protein works to inhibit DNA gyrase. That will leads to the commit suicide of bacteria.