Difference between revisions of "Team:Hong Kong HKUST/Safety"

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<p>Please visit <a href="https://2016.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<b>Safety</b>
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</h1>  
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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<blockquote><p>Mainly focusing on the investigation of the properties and the future usages of the Tristable Switch, we did not have to release the bacterial chassis to the field. In the meantime, prioritizing safety of our project, we strictly followed all the instructions from the iGEM Headquarter and our school to avoid causing any potential risks.</p></blockquote>
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<h2 class="text-muted"><b><em>ORGANISM SAFETY<b></em></h2>
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<h5>Safe Project Design</h5>
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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<li>Choosing a non-pathogenic chassis</li>
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<th>Species name (including strain)</th>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<th>Risk Group</th>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<th>Source</th>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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<th>Human Risks</th>
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<th>Aquisition</th>
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<th>Use</th>
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<h5>Safe Lab Work</h5>
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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<td width="5%"><i>E. coli</i> <br>(DH10B)</td>
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<td width="5%">Group 2</td>
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<a href="http://tools.thermofisher.com/content/sfs/msds/2013/500015_MTR-NALT_EN.pdf" target="_blank"> http://tools.thermofisher.com/content/sfs<br>/msds/2013/500015_MTR-NALT_EN.pdf<br>
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013/500015_MTR-NALT_EN.pdf<br></a>
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<td width="35%"">Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases.</td>
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<td width="15%">We acquired from Professor King Lau Chow's lab in HKUST </td>
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<td width="15%">As a chassis for experiments and cloning
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<td><i>E. coli</i> <br>(JW0336-1)</td>
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<td>Group 2</td>
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<td><a href= "http://cgsc.biology.yale.edu/StrainRpt.php?ID=106809/" target="_blank"> http://cgsc.biology.yale.edu/StrainRpt.php?ID=106809/ </a>
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<td> Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases..</td>
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<td> It was brought from the <i>E. coli</i> Genetic Resource Center.-</td>
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<td> As a chassis for experiments and characterization </td>
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<h5>Safe Shipment</h5>
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<h2 class="text-muted"><b><em>UNIVERSITY SAFETY</b></em></h2>
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<p>Health, Safety & Environment Office (HSEO) is responsible for safety regulations at our institution, the Hong Kong University of Science and Technology, HKUST. All of the team members absolutely complied with the guidelines from the HSEO so as to ensure the safety inside and outside the laboratory. <br><br>
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The biosafety guideline of our institution can be directed to <a href = "http://www.ab.ust.hk/hseo/sm06/ch9.htm " target="_blank">here</a>.<br><br>
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The regulations that govern biosafety in research laboratories in Hong Kong can be directed to <a href="http://www.legislation.gov.hk/blis_pdf.nsf/6799165D2FEE3FA94825755E0033
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E532/CD005BD8FCD84653482576EA0053077B/$FILE/CAP_607_e_b5.pdf" target="_blank">here</a>.</p>
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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<b><em>SAFETY TRAINING</em></b>
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<p>This year's HKUST iGEM team members received safety training before the start of our project. Topics that we learned about in the safety training are as follows:<br><br>
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1. Biological Safety <br>
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2. Chemical Safety I/ Chemical Safety for Laboratory Users <br>
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3. Chemical Safety II/ Hazardous Waste Management <br><br>
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The link to the laboratory safety training requirements of our institution (The Hong Kong University of Science and Technology, HKUST) can be directed to <a href = "http://www.ab.ust.hk/hseo/training.htm" target="_blank">here</a>.</p>  
 
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<b><em>REFERENCES</em></b>
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  <li>Chapter 9 Biological Safety. (1 November, 2006). Retrieved 3 September, 2016, from
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<a href = "http://www.ab.ust.hk/hseo/sm06/ch9.htm " target="_blank">http://www.ab.ust.hk/hseo/sm06/ch9.htm </a></li>
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  <li>Health, Safety and Environmental Office. (2 October, 2015). Retrieved 3 September, 2016, from <a href = "http://www.ab.ust.hk/hseo/training.htm" target="_blank">http://www.ab.ust.hk/hseo/training.htm</a></li>
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Latest revision as of 03:31, 20 October 2016

Home

Safety

Mainly focusing on the investigation of the properties and the future usages of the Tristable Switch, we did not have to release the bacterial chassis to the field. In the meantime, prioritizing safety of our project, we strictly followed all the instructions from the iGEM Headquarter and our school to avoid causing any potential risks.

ORGANISM SAFETY


Species name (including strain) Risk Group Source Human Risks Aquisition Use
E. coli
(DH10B)		 Group 2 http://tools.thermofisher.com/content/sfs
/msds/2013/500015_MTR-NALT_EN.pdf
013/500015_MTR-NALT_EN.pdf
 		Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases. We acquired from Professor King Lau Chow's lab in HKUST As a chassis for experiments and cloning
E. coli
(JW0336-1)		 Group 2 http://cgsc.biology.yale.edu/StrainRpt.php?ID=106809/ Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases.. It was brought from the E. coli Genetic Resource Center.- As a chassis for experiments and characterization

UNIVERSITY SAFETY


Health, Safety & Environment Office (HSEO) is responsible for safety regulations at our institution, the Hong Kong University of Science and Technology, HKUST. All of the team members absolutely complied with the guidelines from the HSEO so as to ensure the safety inside and outside the laboratory.

The biosafety guideline of our institution can be directed to here.

The regulations that govern biosafety in research laboratories in Hong Kong can be directed to here.

SAFETY TRAINING


This year's HKUST iGEM team members received safety training before the start of our project. Topics that we learned about in the safety training are as follows:

1. Biological Safety
2. Chemical Safety I/ Chemical Safety for Laboratory Users
3. Chemical Safety II/ Hazardous Waste Management

The link to the laboratory safety training requirements of our institution (The Hong Kong University of Science and Technology, HKUST) can be directed to here.

REFERENCES

  1. Chapter 9 Biological Safety. (1 November, 2006). Retrieved 3 September, 2016, from http://www.ab.ust.hk/hseo/sm06/ch9.htm
  2. Health, Safety and Environmental Office. (2 October, 2015). Retrieved 3 September, 2016, from http://www.ab.ust.hk/hseo/training.htm