Difference between revisions of "Team:Hong Kong HKUST/Safety"

 
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</head>
 
</head>
 
<body>
 
<body>
  
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<ul id="navlist">
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<section class="section jumbotron">
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  <li id="default"><a href="https://2016.igem.org/Team:Hong_Kong_HKUST/Safety"></a></li>
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<div class="content_wrapper_neo">
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  <li id="tour" style="display:block;"></li>
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<h1 class="title text-center">
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  <li id="circuit" style="display:block;"></li>
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<b>Safety</b>
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</ul>
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</h1>  
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<div id="circuit-overlay"><img id="circuitFig" src="https://static.igem.org/mediawiki/2016/9/91/T--Hong_Kong_HKUST--construct_mode.png"></div>
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<blockquote><p>Mainly focusing on the investigation of the properties and the future usages of the Tristable Switch, we did not have to release the bacterial chassis to the field. In the meantime, prioritizing safety of our project, we strictly followed all the instructions from the iGEM Headquarter and our school to avoid causing any potential risks.</p></blockquote>
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                </div>
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<div id="tour-overlay">
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         </section>  
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  <div class="tourdiv" id="tour_none" style="z-index: 1;">
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<section class="section">
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<div class="content_wrapper_neo">
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      <h1> Navigation</h1>
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<h2 class="text-muted"><b><em>ORGANISM SAFETY<b></em></h2>
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      <p>Before boarding, go check out below some tricks that will be useful in the course of your journey!</p>
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<br>
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      <hr>
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<div class="table-responsive">
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      <img src="https://static.igem.org/mediawiki/2016/b/b4/T--Hong_Kong_HKUST--Copyoftrafficlight.svg" alt="trafficlight" style="float: right;">
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      <h2>Trick 1 - The Mode Switcher</h2>
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      <p1>The mode switcher, in analogue to a traffic light, combines 3 different interfaces into one powerful device switchable to one another. <br><br> Red: <b>Default mode</b> <small>(Veteran, no support needed!)</small><br> Yellow: <b>Tour mode</b> <small>(Summary of each and every page in one single map)</small>  <br> Green: <b>Construct mode</b> <small>(A reference diagram of our genetic construction)</small></p1>
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      <br>
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      <button type="button" onclick="tour_page()">Proceed</button>
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  </div>
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  <div class="tourdiv"  style="z-index: 2;" id="tour_next">
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      <img src="https://static.igem.org/mediawiki/2016/2/28/T--Hong_Kong_HKUST--route_map.svg" alt="TourMode" usemap="#RouteMap" style="margin-left: 10%; width:75%; height: 75%;">
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      <map name="RouteMap">
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<area shape="circle" coords="242 126 20" alt="Home" href="https://2016.igem.org/Team:Hong_Kong_HKUST/Danson" onmouseover="tourFunction_home()" onmouseout="tourFunction_home()">
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<area shape="circle" coords="333 126 20" alt="About" href="https://2016.igem.org/Team:Hong_Kong_HKUST/The_Team" onmouseover="tourFunction_about()" onmouseout="tourFunction_home()">
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<area shape="circle" coords="429 126 20" alt="Project" href="https://2016.igem.org/Team:Hong_Kong_HKUST/Project_Overview" onmouseover="tourFunction_project()" onmouseout="tourFunction_home()">
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<area shape="circle" coords="530 126 20" alt="Achievements" href="https://2016.igem.org/Team:Hong_Kong_HKUST/Parts_Submitted" onmouseover="tourFunction_achievements()" onmouseout="tourFunction_home()">
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         <area shape="circle" coords="623 126 20" alt="Future_Plan" href="https://2016.igem.org/Team:Hong_Kong_HKUST/Future_Plan" onmouseover="tourFunction_future_plan()" onmouseout="tourFunction_home()">
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<area shape="circle" coords="427 43 20" alt="Human Practices" href="https://2016.igem.org/Team:Hong_Kong_HKUST/HP_Intro" onmouseover="tourFunction_human_practices()" onmouseout="tourFunction_home()">
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<area shape="circle" coords="335 220 20" alt="Modelling" href="https://2016.igem.org/Team:Hong_Kong_HKUST/Modelling" onmouseover="tourFunction_modelling()" onmouseout="tourFunction_home()">
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<area shape="circle" coords="335 295 20" alt="Lab" href="hhttps://2016.igem.org/Team:Hong_Kong_HKUST/Protocols_Logbook" onmouseover="tourFunction_lab()" onmouseout="tourFunction_home()">
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<area shape="circle" coords="242 295 20" alt="Interlab" href="https://2016.igem.org/Team:Hong_Kong_HKUST/Interlab" onmouseover="tourFunction_interlab()" onmouseout="tourFunction_home()">
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<area shape="circle" coords="531 223 20" alt="Collaboration" href="https://2016.igem.org/Team:Hong_Kong_HKUST/Collab" onmouseover="tourFunction_collaboration()" onmouseout="tourFunction_home()">
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      </map>
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      <p id="tourPar">Welcome to HKUST 2016 iGEM team - Troika!!  We made a simple tour to make navigating our website a breeze! Follow us!</p>
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  </div>
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</div>
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<div class="content_wrapper" >
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<h1><b>Safety</b></h1>
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<hs>Organisms Safety</hs>
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<table id="safety_table" border="2px" align="center">
 
<table id="safety_table" border="2px" align="center">
 
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</tr></table>
 
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</div>
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<br><br><br>
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<hs>Project Safety</hs>
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        </section>
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<p>Mainly focusing on the investigation of the properties and the future usages of the tri-stable switch, we did not have to release it to the field. In the meantime, prioritizing safety of our project, we strictly followed all the instructions from the iGEM Headquarter and our school to avoid causing any potential risks.</p>
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<br><br><br>
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<section class="section jumbotron">
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<hs>University Safety</hs>
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<div class="content_wrapper_neo">
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<h2 class="text-muted"><b><em>UNIVERSITY SAFETY</b></em></h2>
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<br>
 
<p>Health, Safety & Environment Office (HSEO) is responsible for safety regulations at our institution, the Hong Kong University of Science and Technology, HKUST. All of the team members absolutely complied with the guidelines from the HSEO so as to ensure the safety inside and outside the laboratory. <br><br>
 
<p>Health, Safety & Environment Office (HSEO) is responsible for safety regulations at our institution, the Hong Kong University of Science and Technology, HKUST. All of the team members absolutely complied with the guidelines from the HSEO so as to ensure the safety inside and outside the laboratory. <br><br>
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The biosafety guideline of our institution can be directed to <a href = "http://www.ab.ust.hk/hseo/sm06/ch9.htm " target="_blank">here</a><br><br>
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The biosafety guideline of our institution can be directed to <a href = "http://www.ab.ust.hk/hseo/sm06/ch9.htm " target="_blank">here</a>.<br><br>
 
The regulations that govern biosafety in research laboratories in Hong Kong can be directed to <a href="http://www.legislation.gov.hk/blis_pdf.nsf/6799165D2FEE3FA94825755E0033
 
The regulations that govern biosafety in research laboratories in Hong Kong can be directed to <a href="http://www.legislation.gov.hk/blis_pdf.nsf/6799165D2FEE3FA94825755E0033
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E532/CD005BD8FCD84653482576EA0053077B/$FILE/CAP_607_e_b5.pdf" target="_blank">here</a></p><br><br><br>
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E532/CD005BD8FCD84653482576EA0053077B/$FILE/CAP_607_e_b5.pdf" target="_blank">here</a>.</p>
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<hs>Safety Training Courses</hs>
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                </div>
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        </section>
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<section class="section">
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<div class="content_wrapper_neo">
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<h2 class="text-muted">
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<b><em>SAFETY TRAINING</em></b>
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</h2> 
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<br>
 
<p>This year's HKUST iGEM team members received safety training before the start of our project. Topics that we learned about in the safety training are as follows:<br><br>
 
<p>This year's HKUST iGEM team members received safety training before the start of our project. Topics that we learned about in the safety training are as follows:<br><br>
 
1. Biological Safety <br>
 
1. Biological Safety <br>
 
2. Chemical Safety I/ Chemical Safety for Laboratory Users <br>
 
2. Chemical Safety I/ Chemical Safety for Laboratory Users <br>
 
3. Chemical Safety II/ Hazardous Waste Management <br><br>
 
3. Chemical Safety II/ Hazardous Waste Management <br><br>
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The link to the laboratory safety training requirements of our institution (The Hong Kong University of Science and Technology, HKUST) can be directed to <a href = "http://www.ab.ust.hk/hseo/training.htm" target="_blank">here</a></p>
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The link to the laboratory safety training requirements of our institution (The Hong Kong University of Science and Technology, HKUST) can be directed to <a href = "http://www.ab.ust.hk/hseo/training.htm" target="_blank">here</a>.</p>  
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<br><br><br>
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<h3> Reference </h3>
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<p2><br>
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1. Chapter 9 Biological Safety. (1 November, 2006). Retrieved 3 September, 2016, from
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<a href = "http://www.ab.ust.hk/hseo/sm06/ch9.htm " target="_blank">http://www.ab.ust.hk/hseo/sm06/ch9.htm </a>
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<br><br>
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2. Health, Safety and Environmental Office. (2 October, 2015). Retrieved 3 September, 2016, from <a href = "http://www.ab.ust.hk/hseo/training.htm" target="_blank">http://www.ab.ust.hk/hseo/training.htm</a>
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</p2>
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</div>
 
</div>
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</section>
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<section class="section jumbotron">
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<div class="content_wrapper_neo">
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<h2 class="text-muted">
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<b><em>REFERENCES</em></b>
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</h2> 
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<ol class="btn-responsive">
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  <li>Chapter 9 Biological Safety. (1 November, 2006). Retrieved 3 September, 2016, from
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<a href = "http://www.ab.ust.hk/hseo/sm06/ch9.htm " target="_blank">http://www.ab.ust.hk/hseo/sm06/ch9.htm </a></li>
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  <li>Health, Safety and Environmental Office. (2 October, 2015). Retrieved 3 September, 2016, from <a href = "http://www.ab.ust.hk/hseo/training.htm" target="_blank">http://www.ab.ust.hk/hseo/training.htm</a></li>
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</ol>
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                </div>
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        </section>
  
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<script>
 
<script>
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function tour_page() {
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    $(document).ready(function(){
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    document.getElementById("tour_none").style.display ="none";
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         $("div#ustMenuWrapper li#ustLabTab").addClass("ustActiveTab");
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}
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//Tour mode Effect of changing content
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function tourFunction_home() {
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         document.getElementById("tourPar").innerHTML = "Welcome to HKUST 2016 iGEM team - Troika!!";
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}
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function tourFunction_about(){
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document.getElementById("tourPar").innerHTML = "Let's meet our members and see how division of labour works out in our team! Also, check out the attributed parties of our project if you may.";
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}
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function tourFunction_project(){
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document.getElementById("tourPar").innerHTML = "Get to know our project here! We built a biological device termed as tri-stable switch that could perform three discrete but alternating, steady states driven by three different repressible promoters. What's more, you can find a schematic diagram of the genetic switch by clicking on the green light button on the top right corner for your reference!";
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function tourFunction_achievements(){
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document.getElementById("tourPar").innerHTML = "We list out here the medal requirements that we have fulfilled, with other additional achievements such as new parts that we submitted to the Registry.";
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}
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function tourFunction_future_plan(){
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document.getElementById("tourPar").innerHTML = "You can have a grasp of how we are to sustain our project in the future as well as how an add-on investigation on the transcriptional manipulation in collaboration with the Rice University was conducted.";
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}
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function tourFunction_human_practices(){
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document.getElementById("tourPar").innerHTML = "For the outreach part, our main focus is on designing an applicable product of the tri-stable switch that is intended for public convenient use. Other missions that we have accomplished so far include educating high school students, exploring intellectual property right policies, and assisting other iGEM teams in plan-making.";
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function tourFunction_modelling(){
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document.getElementById("tourPar").innerHTML = "To evaluate our works, we identified the parameters potentially affecting our design, predict, and verify the consistency of our experimental results by bringing about a mathematical prediction model and a stability analysis.";
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}
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function tourFunction_lab(){
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document.getElementById("tourPar").innerHTML = "We have compiled our logbooks together with the protocols we employed in this page. Safety information is also provided.";
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}
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function tourFunction_interlab(){
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document.getElementById("tourPar").innerHTML = "We joined this year's iGEM Interlab Measurement Study where we conducted a relative expression compression by green fluorescence measurement.";
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}
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function tourFunction_collaboration(){
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document.getElementById("tourPar").innerHTML = "This year, we collaborate with two institutions, NUS and IIT in respective areas.";
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}
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<!--<div class="column full_size">
 
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<p>Please visit <a href="https://2016.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
 
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
 
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<div class="column full_size">
 
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<h5>Safe Project Design</h5>
 
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
 
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<ul>
 
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<li>Choosing a non-pathogenic chassis</li>
 
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<li>Choosing parts that will not harm humans / animals / plants</li>
 
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
 
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<li>Including an "induced lethality" or "kill-switch" device</li>
 
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</ul>
 
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</div>
 
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<div class="column half_size">
 
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<h5>Safe Lab Work</h5>
 
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
 
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</div>
 
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<div class="column half_size">
 
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<h5>Safe Shipment</h5>
 
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
 
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</div>
 
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</html>-->
 

Latest revision as of 03:31, 20 October 2016

Home

Safety

Mainly focusing on the investigation of the properties and the future usages of the Tristable Switch, we did not have to release the bacterial chassis to the field. In the meantime, prioritizing safety of our project, we strictly followed all the instructions from the iGEM Headquarter and our school to avoid causing any potential risks.

ORGANISM SAFETY


Species name (including strain) Risk Group Source Human Risks Aquisition Use
E. coli
(DH10B)		 Group 2 http://tools.thermofisher.com/content/sfs
/msds/2013/500015_MTR-NALT_EN.pdf
013/500015_MTR-NALT_EN.pdf
 		Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases. We acquired from Professor King Lau Chow's lab in HKUST As a chassis for experiments and cloning
E. coli
(JW0336-1)		 Group 2 http://cgsc.biology.yale.edu/StrainRpt.php?ID=106809/ Potentially harmful when inhaled or swallowed. Can cause eye and skin irritation in rare cases.. It was brought from the E. coli Genetic Resource Center.- As a chassis for experiments and characterization

UNIVERSITY SAFETY


Health, Safety & Environment Office (HSEO) is responsible for safety regulations at our institution, the Hong Kong University of Science and Technology, HKUST. All of the team members absolutely complied with the guidelines from the HSEO so as to ensure the safety inside and outside the laboratory.

The biosafety guideline of our institution can be directed to here.

The regulations that govern biosafety in research laboratories in Hong Kong can be directed to here.

SAFETY TRAINING


This year's HKUST iGEM team members received safety training before the start of our project. Topics that we learned about in the safety training are as follows:

1. Biological Safety
2. Chemical Safety I/ Chemical Safety for Laboratory Users
3. Chemical Safety II/ Hazardous Waste Management

The link to the laboratory safety training requirements of our institution (The Hong Kong University of Science and Technology, HKUST) can be directed to here.

REFERENCES

  1. Chapter 9 Biological Safety. (1 November, 2006). Retrieved 3 September, 2016, from http://www.ab.ust.hk/hseo/sm06/ch9.htm
  2. Health, Safety and Environmental Office. (2 October, 2015). Retrieved 3 September, 2016, from http://www.ab.ust.hk/hseo/training.htm