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                                     <div class="texttitle">Abstract</div>
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                                    <p class="lead add-bottom" style="color:#5E5656">The problem of uranium contamination is a source of great concern. Uranium could have severe detrimental health effects (it is particularly harmful to the liver, kidney and bones) and lead to environment issues (chemical and radioactive hazards). <a href="https://2016.igem.org/Team:Peking/HP/311">Current treatment options</a> available for uranium leaks in nuclear power plants or uranium pollution around ore-fields, such as ion exchange, flocculation-setting and phytoremediation, all have limitations including their high cost, low efficiency and the sheer complexity of the involved procedures.</p>
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<p style="text-align:justify; text-justify:inter-ideograph;><span style="text-indent:21pt;font-family:'Times New Roman';letter-spacing:0pt;font-size:14pt">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;In 2016, we focused on to control mosquitoes. We cloned various&nbsp;</span><i style="text-indent:21pt"><span style="font-family:'Times New Roman';letter-spacing:0pt;font-size:14pt">Cry</span></i><span style="text-indent:21pt;font-family:'Times New Roman';letter-spacing:0pt;font-size:14pt">&nbsp;and&nbsp;</span><i style="text-indent:21pt"><span style="font-family:'Times New Roman';letter-spacing:0pt;font-size:14pt">Cyt</span></i><span style="text-indent:21pt;font-family:'Times New Roman';letter-spacing:0pt;font-size:14pt">&nbsp;toxin genes from&nbsp;</span><i style="text-indent:21pt"><span style="font-family:'Times New Roman';letter-spacing:0pt;font-size:14pt">LLP29, Bacillus thuringiensis&nbsp;</span></i><span style="text-indent:21pt;font-family:'Times New Roman';letter-spacing:0pt;font-size:14pt">strain. We also constructed Cry and Cyt toxin parts based on option optimization of&nbsp;</span><i style="text-indent:21pt"><span style="font-family:'Times New Roman';letter-spacing:0pt;font-size:14pt">Chlamydomonas reinhardtii.</span></i><span style="text-indent:21pt;font-family:'Times New Roman';letter-spacing:0pt;font-size:14pt">Meanwhile, we built toxin groups express device based on 2A peptide system. And we tested their toxicity to the larvae of various genus of mosquitoes under the safe lab environment. Considering the potential problems in the natural environment, BNU-China and ZJU-China helped us to build the growth and toxicity model to estimate the natural conditions. To introduce the practical knowledge about how to prevent and control mosquitoes, we designed a handbook covering this topic and sent it to Zambia to help local people who are trouble in mosquitoes. We are proud of our project!</span></p>
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                                    <p class="lead add-bottom" style="color:#5E5656">To address these problems, the Peking iGEM team aims to construct <a href="https://2016.igem.org/Team:Peking/Description">a novel functional biomaterial</a> consisting of multiple functional protein modules. This material is designed to be produced and secreted by bacteria, and self-assembled to form a polymer network. In combination with a specific <a href="https://2016.igem.org/Team:Peking/Uranyl-adsorption">Super Uranyl-binding Protein</a>, it obtains the ability to adsorb uranyl ions. After very short contacting with polluted water, the uranyl-laden biomaterial, which also contains a <a href="https://2016.igem.org/Team:Peking/Clearance">monomeric streptavidin module</a>, could be easily retrieved using biotinylated magnetic beads.
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                                    <p class="lead add-bottom" style="color:#5E5656">This uranyl-binding biomaterial shows a series of advantages, such as high specificity, high efficiency, self-assembly and renewability. Furthermore, the uranyl-binding module could be replaced or combined with modules that are capable of binding other heavy metal ions, as well as fluorescent proteins, obtaining <a href="https://2016.igem.org/Team:Peking/Proof">multi-functionality</a>. By taking advantage of modularization in the design, additional applications beyond uranium adsorption could be developed based on this material in the future.</p>
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Latest revision as of 03:41, 20 October 2016

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Achievement

        In 2016, we focused on to control mosquitoes. We cloned various Cry and Cyt toxin genes from LLP29, Bacillus thuringiensis strain. We also constructed Cry and Cyt toxin parts based on option optimization of Chlamydomonas reinhardtii.Meanwhile, we built toxin groups express device based on 2A peptide system. And we tested their toxicity to the larvae of various genus of mosquitoes under the safe lab environment. Considering the potential problems in the natural environment, BNU-China and ZJU-China helped us to build the growth and toxicity model to estimate the natural conditions. To introduce the practical knowledge about how to prevent and control mosquitoes, we designed a handbook covering this topic and sent it to Zambia to help local people who are trouble in mosquitoes. We are proud of our project!