Difference between revisions of "Team:Tokyo Tech/Toxin Assay/Overview"

 
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<h1 align="center">3-1-0. Overview</h1>
 
<h1 align="center">3-1-0. Overview</h1>
 
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<h3 class="link"><a href="#TA">3-1-0. TA system</a></h3>
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<h3 class="link"><a href="#AoM">3-1-1.　Adjustment of MazF expression</a></h3>
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<h3 class="link"><a href="#Sys">3-1-2. <span style="font-style : italic">mazEF</span> System Assay</a></h3>
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<h3 class="link"><a href="#SaG"><font size="2.7">&nbsp;&nbsp;&nbsp;3-1-2-1. Stop & Go</font></a></h3>
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<h3 class="link"><a href="#GaS"><font size="2.7">&nbsp;&nbsp;&nbsp;3-1-2-2. Go & Stop</font></a></h3>
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<h3 class="link"><a href="#CoC">3-1-3. Control of Cell Growth</a></h3>
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<h3 class="link"><a href="#Ref">3-1-4. Reference</a></h3>
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<h2><span>3-1-0. TA system</span></h2>
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<p class="normal_text">The experiments of iGEM 2016 team Tokyo_Tech are divided into Toxin, AHL, AmiE, and the temperature-dependent promoter Assay. This section describes Toxin Assay.
 
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TA system is a function that controls cell growth on the genomic DNA of many microorganisms.Preceding iGEM teams studied on the following TA systems; ccdB-ccdA (iGEM 2014 team ULB-Brussels and iGEM 2013 team UGent), Kid-Kis, Zeta-Epsilon(iGEM 2014 team Wageningen UR) and Hok-Soc(iGEM 2015 team UMaryland). Almost all the iGEM teams dealing with TA system aimed to use it as Kill Switch or substitution for antibiotics. On the other hand, we aim to study the interaction of toxin and antitoxin in a cell. Besides some teams failed to show enough data of TA system assay. Although UCSF 2012 experienced the communication through TA system including mazEF system, they did not introduce toxin and antitoxin into a cell and could not show mazEF system assay data. Our results show that the cell growth can be controlled by mazEF system.
 
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In iGEM 2016 team Tokyo_Tech, we have studied on mazEF system, which has been studied well as the TA system.
 
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<p class="normal_text">The experiments of iGEM 2016 team Tokyo_Tech are divided into Toxin, AHL, AmiE, and temperature-dependent promoter assays. This section describes the toxin assay. Our team studied the<span style ="font-style : italic">mazEF</span> system, which is one of the most studied TA systems.
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<p class="normal_text">TA systems are found on the genomic DNA of many bacteria and they play important roles in controlling the cell growth. Preceding iGEM teams have 
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studied the following TA systems; <span style ="font-style : italic">ccdB &#8208; ccdA</span> (iGEM 2014 team ULB-Brussels and iGEM 2013 team UGent), <span style ="font-style : italic">kid &#8208; kis</span>, <span style ="font-style : italic">zeta &#8208; epsilon</span> (iGEM 2014 team
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Wageningen UR) and <span style ="font-style : italic">hok &#8208; soc</span> (iGEM 2015 team UMaryland) (<a href="#Table. 3-1-0-1.">Table. 3-1-0-1.</a>). Almost all the iGEM teams dealing with TA systems aimed to use them as a kill switch or substitution for antibiotics.<br>
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                                <h3 id="Table. 3-1-0-1."> </h3>
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                                <div align="center"><p class="caption" style="font-size: 16px; text-align: center;"><span style="font-weight: bold;">Table 3-1-0-1 </span> TA system that has been studied in the preceding iGEM.</span>
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<p class="normal_text"> On the other hand, we aimed to study cooperation of toxin and antitoxin in cells. Although UCSF 2012 showed the communication through TA systems including the <span style ="font-style : italic">mazEF</span> system, they did not introduce toxin and antitoxin genes into a single cell and did not show the data on the whole <span style ="font-style : italic">mazEF</span> system. Besides, some preceding teams failed to show enough data on TA systems. Our results show that cell growth can be controlled flexibly by manipulating expression ratio of <span style ="font-style : italic">mazE</span> and <span style ="font-style : italic">mazF</span>, implying new applicability of TA systems to many biotechnological fields and future iGEM projects.</span> <br><br>
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<h2><span>3-1-4. Reference</span></h2>
 
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<p class="normal_text"><br>1) Yonglong Zhang, Junjie Zhang, Klaus P. Hoeflich, Mitsuhiko Ikura, Guoliang Qing, and Masayori Inouye, MazF Cleaves Cellular mRNAs Specifically at ACA to Block Protein Synthesis in <span style="font-style : italic">Escherichia coli</span>. Molecular Cell, Vol. 12, 913–923.<br>
 
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2) Hazan, R., B. Sat, and H. Engelberg-Kulka. <span style="font-style : italic">Escherichia coli mazEF</span>mediated cell death is triggered by various stressful conditions. J. Bacteriol.186:3663–3669.
 
  
 
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<p class="normal_text">Jump to <a href="https://2016.igem.org/Team:Tokyo_Tech/Toxin_Assay/Adjustment_of_Expression_of_MazF"> Adjustment of MazF Expression</a> page.</a>
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Latest revision as of 11:51, 6 November 2016

3-1-0. Overview

The experiments of iGEM 2016 team Tokyo_Tech are divided into Toxin, AHL, AmiE, and temperature-dependent promoter assays. This section describes the toxin assay. Our team studied themazEF system, which is one of the most studied TA systems.

TA systems are found on the genomic DNA of many bacteria and they play important roles in controlling the cell growth. Preceding iGEM teams have studied the following TA systems; ccdB ‐ ccdA (iGEM 2014 team ULB-Brussels and iGEM 2013 team UGent), kid ‐ kis, zeta ‐ epsilon (iGEM 2014 team Wageningen UR) and hok ‐ soc (iGEM 2015 team UMaryland) (Table. 3-1-0-1.). Almost all the iGEM teams dealing with TA systems aimed to use them as a kill switch or substitution for antibiotics.

Table 3-1-0-1 TA system that has been studied in the preceding iGEM.



On the other hand, we aimed to study cooperation of toxin and antitoxin in cells. Although UCSF 2012 showed the communication through TA systems including the mazEF system, they did not introduce toxin and antitoxin genes into a single cell and did not show the data on the whole mazEF system. Besides, some preceding teams failed to show enough data on TA systems. Our results show that cell growth can be controlled flexibly by manipulating expression ratio of mazE and mazF, implying new applicability of TA systems to many biotechnological fields and future iGEM projects.

Jump to Adjustment of MazF Expression page.