Difference between revisions of "Team:OUC-China/Safety"

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<p>Please visit <a href="https://2016.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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<div class="column full_size">
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<h5>Safe Project Design</h5>
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
 
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<ul>
 
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<li>Choosing a non-pathogenic chassis</li>
 
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<li>Choosing parts that will not harm humans / animals / plants</li>
 
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
 
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<li>Including an "induced lethality" or "kill-switch" device</li>
 
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</ul>
 
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</div>
 
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<div class="column half_size">
 
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<h5>Safe Lab Work</h5>
 
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
 
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</div>
 
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<div class="column half_size">
 
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<h5>Safe Shipment</h5>
 
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
 
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<li class="active"><a href="#float01"><span style="font-family:'Lucida Calligraphy';font-size:22px;">S</span>afe Project Design</a></li>
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<li><a href="#float02"><span style="font-family:'Lucida Calligraphy';font-size:22px;">S</span>afe Lab Work</a></li>
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<li><a href="#float03"><span style="font-family:'Lucida Calligraphy';font-size:22px;">S</span>afe Shipment</a></li>
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<br>
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<h3 class="text-center"><img src="https://static.igem.org/mediawiki/2016/c/cf/T--OUC-China--head-icon1.fw.png" alt="icon">SAFE PROJECT DESIGN<img src="https://static.igem.org/mediawiki/2016/f/f8/T--OUC-China--head-icon2.fw.png" alt="icon"></h3>
 +
<br>
 +
<p>Being responsible for our earth and ourselves is our attitude towards every experiment. In our project, all the parts we have utilized are selected from Risk Group 1, none of which are with a Red Flag. What’s more, nonpathogenic bacteria, <i>E.coli.K12</i> is employed as the main chassis for our prior design on the DNA sequence, stem-loop. As a matter of fact, when using stem-loops to tune the expression of genes, it is harmless for the growth of bacteria and relatively safe for industrialized applications. For the sake of environments, all the chassis organisms will be sterilized before being abandoned to prevent the genes from leaking.</p>
 +
<br id="float02">
 +
<hr>
 +
<h3 class="text-center"><img src="https://static.igem.org/mediawiki/2016/c/cf/T--OUC-China--head-icon1.fw.png" alt="icon">SAFE LAB WORK<img src="https://static.igem.org/mediawiki/2016/f/f8/T--OUC-China--head-icon2.fw.png" alt="icon"></h3>
 +
<br>
 +
<p>We works at a neat lab named “Lab for Microinnovation and Enterprise”, which is a BSL-2 laboratory. Before working in the lab, we are supposed to be trained by the skillful hands, not only for the general lab safety rules, but also for the standard experimental operation avoiding unnecessary damage. </p>
 +
<br>
 +
<p>No experiment carries no risk to the experimenters, hence we are properly equipped with facilities, such as latex gloves, nitrile gloves，goggles, lab coats to protect us from biotic and abiotic hazards.</p>
 +
<div class="row">
 +
<div class="col-md-2"></div>
 +
<div class="col-md-8">
 +
<img src="https://static.igem.org/mediawiki/2016/7/70/T--OUC-China--Safety5.jpg" alt="Our personal safety precautions" class="img-responsive"/>
 +
</div>
 +
<div class="col-md-2"></div>
 +
</div>
 +
<p class="text-center" style="font-size:16px;">Figure 1 Our personal safety precautions</p>
 +
<p>Then for lab safety, a routine inspection is performed twice a day by everyone, not only for ensuring normal operation of our lab like supplying experimental consumables and inspecting the running of instruments, but also keeps it away from non-biological hazards, especially the water and electricity. For high efficiency, responsibility implements to every team member.</p>
 +
<div class="row" style="max-width:90%;margin:0 auto;">
 +
<div class="col-md-2"></div>
 +
<div class="col-md-8">
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<img src="https://static.igem.org/mediawiki/2016/6/60/T--OUC-China--satety.jpg" class="img-responsive" alt="Table for routine inspection" />
 +
</div>
 +
<div class="col-md-2"></div>
 +
</div>
 +
<p class="text-center" style="font-size:16px;">Figure 2 Table for routine inspection</p>
 +
<p>Furthermore, several operational zones are divided scientifically for special experiments like RNA electrophoresis and  RT-PCR. <b>And a molecular operation area, an ethidium bromide area and a polyacrylamide gel electrophoresis area are set for difficult operations</b></p>
 +
<div class="row" style="max-width:90%;margin:0 auto;">
 +
<div class="col-md-6">
 +
<img src="https://static.igem.org/mediawiki/2016/5/54/T--OUC-China--Safety2.jpg" class="img-responsive" alt="RNA operation area">
 +
<p class="text-center" style="font-size:16px;">Figure 3 RNA operation area</p>
 +
</div>
 +
<div class="col-md-6">
 +
<img src="https://static.igem.org/mediawiki/2016/8/81/T--OUC-China--Safety3.jpg" class="img-responsive" alt="UV transilluminator">
 +
<p class="text-center" style="font-size:16px;">Figure 4 UV transilluminator</p>
 +
</div>
 +
</div>
 +
<p>For us, lab safety is a hot topic and we have come up with a practical lab safety manual which normalizes our lovely lab.</p>
 +
<div style="font-size:18px;max-width:90%;margin:0 auto;border:2px solid #c0c0c0;border-radius:30px;-moz-border-radius:30px;-webkit-border-radius:30px;">
 +
<h4 class="text-center">Practical lab safety manual</h4>
 +
<p>1.Routine inspections must perform between 6:30-8:00pm and 9:30-11:00pm.<br>2.Lab cleaning must be done twice a week with disinfectant moping the floor for at least 3times.<br>3. Well prepared experiment plans should be written before you start.<br>4.Lab coats and gloves must be worn when conducting experiments.<br>5.Short skirts, shorts, and open shoes must not be worn.<br>6.If you see a colleague doing something dangerous, point it out to him or her.<br>7.Lab coats must not be worn outside laboratories and in public areas.<br>8.Know where safety equipment (eyewash, shower and extinguisher) is located. <br>9.Food/drink is not allowed in laboratories where chemicals are used/ stored.<br>10.Know how to clean up spills of common chemicals and specific chemicals you see, for spilling ethidium bromide dealt with sodium hydroxide.<br>11.Always read the directions before using instruments and read MSDS before handling new chemicals carefully.</p>
 +
</div>
 +
<div class="row">
 +
<div class="col-md-2"></div>
 +
<div class="col-md-8">
 +
<img src="https://static.igem.org/mediawiki/2016/6/6d/T--OUC-China--Safety1.jpg" class="img-responsive" alt="Panorama of our lab" />
 +
</div>
 +
<div class="col-md-2"></div>
 +
</div>
 +
<p class="text-center" style="font-size:16px;">Figure 5 Panorama of our lab</p>
 +
<br id="float03">
 +
<br>
 +
<hr>
 +
<h3 class="text-center"><img src="https://static.igem.org/mediawiki/2016/c/cf/T--OUC-China--head-icon1.fw.png" alt="icon">SAFE SHIPMENT<img src="https://static.igem.org/mediawiki/2016/f/f8/T--OUC-China--head-icon2.fw.png" alt="icon"></h3><br>
 +
<p>For submitting our parts to igem HQ, we first took the initiative to consult Chinese entry-exit inspection and quarantine laws and regulations about shipping DNA from China to the USA, which is forbidden without tedious  red tape. Fortunately, GenScript cooperated with igem HQ forward our part sample to the USA. Ergo, we sealed dried DNA and sent it in the submission kit to GenScript, which clearly labelled as DNA without disguise. Our kit poses no risks and is perfectly safe for shipment.</p>
 +
<br>
 +
</div>
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</div>
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<div class="oucBanner">
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        <h3 class="text-center">Cistrons Concerto</h3>
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</div>
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<div class="row oucInformation">
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<div class="col-md-1"></div>
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<div class="col-md-5">
 +
<h3>Thanks:</h3>
 +
<p>1.Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences</p>
 +
<p>2.NEW ENGLAND Biolabs</p>
 +
<p>3.Genscript</p>
 +
</div>
 +
<div class="col-md-5 Contact">
 +
<h3>Contact us:</h3>
 +
<p><b>E-mail</b>: oucigem@163.com</p>
 +
<p><b>Designed and built</b> by @ Jasmine Chen and @ Zexin Jiao</p>
 +
<p>We are OUC-iGEM
 +
<img src="https://static.igem.org/mediawiki/2016/5/58/T--OUC-China--foot4.png" width="70" height="70" alt="logo-one">
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</body>
 
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Latest revision as of 07:55, 7 November 2016

Safety

safety-banner


iconSAFE PROJECT DESIGNicon


Being responsible for our earth and ourselves is our attitude towards every experiment. In our project, all the parts we have utilized are selected from Risk Group 1, none of which are with a Red Flag. What’s more, nonpathogenic bacteria, E.coli.K12 is employed as the main chassis for our prior design on the DNA sequence, stem-loop. As a matter of fact, when using stem-loops to tune the expression of genes, it is harmless for the growth of bacteria and relatively safe for industrialized applications. For the sake of environments, all the chassis organisms will be sterilized before being abandoned to prevent the genes from leaking.


iconSAFE LAB WORKicon


We works at a neat lab named “Lab for Microinnovation and Enterprise”, which is a BSL-2 laboratory. Before working in the lab, we are supposed to be trained by the skillful hands, not only for the general lab safety rules, but also for the standard experimental operation avoiding unnecessary damage.


No experiment carries no risk to the experimenters, hence we are properly equipped with facilities, such as latex gloves, nitrile gloves，goggles, lab coats to protect us from biotic and abiotic hazards.

Our personal safety precautions

Figure 1 Our personal safety precautions

Then for lab safety, a routine inspection is performed twice a day by everyone, not only for ensuring normal operation of our lab like supplying experimental consumables and inspecting the running of instruments, but also keeps it away from non-biological hazards, especially the water and electricity. For high efficiency, responsibility implements to every team member.

Table for routine inspection

Figure 2 Table for routine inspection

Furthermore, several operational zones are divided scientifically for special experiments like RNA electrophoresis and RT-PCR. And a molecular operation area, an ethidium bromide area and a polyacrylamide gel electrophoresis area are set for difficult operations

RNA operation area

Figure 3 RNA operation area

UV transilluminator

Figure 4 UV transilluminator

For us, lab safety is a hot topic and we have come up with a practical lab safety manual which normalizes our lovely lab.

Practical lab safety manual

1.Routine inspections must perform between 6:30-8:00pm and 9:30-11:00pm.
2.Lab cleaning must be done twice a week with disinfectant moping the floor for at least 3times.
3. Well prepared experiment plans should be written before you start.
4.Lab coats and gloves must be worn when conducting experiments.
5.Short skirts, shorts, and open shoes must not be worn.
6.If you see a colleague doing something dangerous, point it out to him or her.
7.Lab coats must not be worn outside laboratories and in public areas.
8.Know where safety equipment (eyewash, shower and extinguisher) is located.
9.Food/drink is not allowed in laboratories where chemicals are used/ stored.
10.Know how to clean up spills of common chemicals and specific chemicals you see, for spilling ethidium bromide dealt with sodium hydroxide.
11.Always read the directions before using instruments and read MSDS before handling new chemicals carefully.

Panorama of our lab

Figure 5 Panorama of our lab



iconSAFE SHIPMENTicon


For submitting our parts to igem HQ, we first took the initiative to consult Chinese entry-exit inspection and quarantine laws and regulations about shipping DNA from China to the USA, which is forbidden without tedious red tape. Fortunately, GenScript cooperated with igem HQ forward our part sample to the USA. Ergo, we sealed dried DNA and sent it in the submission kit to GenScript, which clearly labelled as DNA without disguise. Our kit poses no risks and is perfectly safe for shipment.


Cistrons Concerto

Thanks:

1.Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences

2.NEW ENGLAND Biolabs

3.Genscript

Contact us:

E-mail: oucigem@163.com

Designed and built by @ Jasmine Chen and @ Zexin Jiao

We are OUC-iGEM logo-one logo-two