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{{:Team:Oxford/Templates/Navi}} | {{:Team:Oxford/Templates/Navi}} | ||
<html> | <html> | ||
− | + | <head> | |
+ | <style> | ||
+ | .rotate { | ||
+ | -ms-transform: rotate(13deg); /* IE 9 */ | ||
+ | -webkit-transform: rotate(13deg); /* Chrome, Safari, Opera */ | ||
+ | transform: rotate(13deg); | ||
+ | } | ||
+ | </style> | ||
+ | </head> | ||
<body> | <body> | ||
− | <div id="banner" style="background-image: url(https://static.igem.org/mediawiki/2016/6/63/Chemicals_page_header_oxford_sam_2016.jpeg);"></div> | + | <div id="banner" style="background-image: url(https://static.igem.org/mediawiki/2016/thumb/6/63/Chemicals_page_header_oxford_sam_2016.jpeg/800px-Chemicals_page_header_oxford_sam_2016.jpeg);"></div> |
<div class="container-fluid content-main"> | <div class="container-fluid content-main"> | ||
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<nav class="col-md-3 bs-docs-sidebar navFont"> | <nav class="col-md-3 bs-docs-sidebar navFont"> | ||
<ul id="sidebar" class="nav nav-stacked" data-spy="affix" data-offset-top="330"> | <ul id="sidebar" class="nav nav-stacked" data-spy="affix" data-offset-top="330"> | ||
− | <li> | + | <li><a href="#Chemicals">Chemicals</a> |
− | <a href="# | + | <ul class="nav nav-stacked"> |
+ | <li><a href="#Ethidium-Bromide">Ethidium Bromide</a></li> | ||
+ | <li><a href="#TBE-Buffer">TBE Buffer</a></li> | ||
+ | <li><a href="#Copper-Sulfate-Pentahydrate">Copper Sulphate Pentahydrate</a></li> | ||
+ | <li><a href="#Agarose">Agarose</a></li> | ||
+ | <li><a href="#L-Arabinose">L-Arabinose</a></li> | ||
+ | <li><a href="#BCA-Salt">BCA Salt</a></li> | ||
+ | <li><a href="#Sodium-Ascorbate">Sodium Ascorbate</a></li> | ||
+ | <li><a href="#L-Glutathione">L-Glutathione</a></li> | ||
+ | <li><a href="#Chloramphenicol">Chloramphenicol</a></li> | ||
+ | <li><a href="#Ampicillin">Ampicillin</a></li> | ||
+ | <li><a href="#Tris">Tris</a></li> | ||
+ | <li><a href="#Glycerol">Glycerol</a></li> | ||
+ | <li><a href="#DL-Dithiothreitol">D/L-Dithiothreitol</a></li> | ||
+ | <li><a href="#Imidiazole">Imidiazole</a></li> | ||
+ | <li><a href="#Nickel-Affinity-Beads">Nickel Affinity Beads</a></li> | ||
+ | <li><a href="#Sodium-Alginate">Sodium Alginate</a></li> | ||
+ | </ul> | ||
+ | <li><a href="#Cont">(...cont'd)</a> | ||
+ | <ul class="nav nav-stacked"> | ||
+ | <li><a href="#Crystal-Violet">Crystal Violet</a></li> | ||
+ | <li><a href="#Lysogeny-Broth">Lysogeny Broth</a></li> | ||
+ | <li><a href="#Phosphate-Buffered-Saline">Phosphate Buffered Saline</a></li> | ||
+ | <li><a href="#Sodium-Dodecyl-Sulfate">Sodium Dodecyl Sulphate</a></li> | ||
+ | <li><a href="#Ethanol">Ethanol</a></li> | ||
+ | <li><a href="#Transfection-Buffer-I">Transfection Buffer I</a></li> | ||
+ | <li><a href="#Transfection-Buffer-II">Transfection Buffer II </a></li> | ||
+ | <li><a href="#Chitosan">Chitosan</a></li> | ||
+ | <li><a href="#Instant-Blue">Instant Blue</a></li> | ||
+ | <li><a href="#NEB-Purple-Loading-Dye">NEB Purple Loading Dye</a></li> | ||
+ | <li><a href="#Protease-Inhibitors">Protease Inhibitors</a></li> | ||
+ | <li><a href="#Hydrochloric-Acid">Hydrochloric Acid</a></li> | ||
+ | <li><a href="#Sodium-Hydroxide">Sodium Hydroxide</a></li> | ||
+ | <li><a href="#Monobasic-Sodium-Phosphate">Monobasic Sodium Phosphate</a></li> | ||
+ | <li><a href="#Glacial-Acetic-Acid">Glacial Acetic Acid</a></li> | ||
+ | <li><a href="#Calcium-Chloride">Calcium Chloride</a></li> | ||
+ | </ul> | ||
</li> | </li> | ||
− | + | </nav> | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
<div class="col-md-9 content-right" style="background-color: #fff;"> | <div class="col-md-9 content-right" style="background-color: #fff;"> | ||
− | < | + | <div id="Chemicals" class="pageTitle pageTitleBlue">Chemicals</div> |
+ | <p>A variety of different chemicals were used for the execution of the wet lab experiments. The following list contains all the major chemicals used in our copper chelation system and delivery mechanism studies. Hazardous or dangerous chemicals have also a list of precautionary and hazardous statements as defined by the <a href="http://www.unece.org/trans/danger/publi/ghs/ghs_welcome_e.html">Globally Harmonized System of Classification and Labelling of Chemicals (GHS)</a></p> | ||
+ | |||
+ | <!-- Ethidium Bromide --> | ||
+ | |||
+ | <h2 id="Ethidium-Bromide">Ethidium Bromide</h2> | ||
+ | <div class="div50" style="padding-top: 50px;"> | ||
+ | <p><u>Use:</u> Staining DNA electrophoresis gels.</p> | ||
+ | <p><u>Signal word:</u> Danger</p> | ||
+ | </div> | ||
+ | <div class="div50"> | ||
+ | <img src="https://static.igem.org/mediawiki/2016/0/02/T--Oxford--ETHIDIUMBROMIDE.png" width="100%"/> | ||
+ | </div> | ||
+ | <br style="clear: both"> | ||
+ | <p style="text-align:center"><strong>Hazard statement(s)</strong></p> | ||
+ | <ul> | ||
+ | <li>H302 Harmful if swallowed.</li> | ||
+ | <li>H330 Fatal if inhaled.</li> | ||
+ | <li>H341 Suspected of causing genetic defects.</li> | ||
+ | </ul> | ||
+ | <p style="text-align:center"><strong>Precautionary statement(s)</strong></p> | ||
+ | <ul> | ||
+ | <li>P260 Do not breathe dust/fume/gas/mist/vapours/spray.</li> | ||
+ | <li>P281 Use personal protective equipment as required.</li> | ||
+ | <li>P284 Wear respiratory protection.</li> | ||
+ | <li>P310 Immediately call a POISON CENTER or doctor/ physician.</li> | ||
+ | </ul> | ||
− | |||
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− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
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<!-- TBE --> | <!-- TBE --> | ||
− | <h2>TBE Buffer pH 8.3</h2> | + | |
− | <p>Use: General buffer for DNA experiments.</p> | + | <h2 id="TBE-Buffer">TBE Buffer pH 8.3</h2> |
− | <p>Non-Hazardous</p> | + | <div class="div50" style="padding-top: 30px"> |
+ | <p><u>Use:</u> General buffer for DNA experiments.<br>Made of three chemicals: Tris, borate and EDTA</p> | ||
+ | <p>Non-Hazardous</p> | ||
+ | </div> | ||
+ | <div class="div50"> | ||
+ | <img src="https://static.igem.org/mediawiki/2016/4/47/T--Oxford--TBE.gif" style="float: right" width="90%"/> | ||
+ | </div> | ||
+ | <br style="clear: both"> | ||
+ | |||
<!-- Copper Sulphate --> | <!-- Copper Sulphate --> | ||
− | <h2>Copper Sulphate Pentahydrate | + | |
− | <p>Use: Induction of copper | + | <h2 style="clear: both" id="Copper-Sulfate-Pentahydrate" style="align: left;">Copper Sulphate Pentahydrate</h2> |
− | <p>Signal word: Warning</p> | + | <div class="div50" style="padding-top: 50px"> |
− | <p style="text-align:center"><strong>Hazard statement(s)</strong></p> | + | <p><u>Use:</u> Induction of copper promoters and Cu2+ chelation assays.</p> |
− | <ul> | + | <p><u>Signal word:</u> Warning</p> |
− | <li>H302 Harmful if swallowed.</li> | + | </div> |
− | <li>H315 Causes skin irritation.</li> | + | <div class="div50"> |
− | <li>H319 Causes serious eye irritation.</li> | + | <img src="https://static.igem.org/mediawiki/2016/b/b6/T--Oxford--CUSO4H2O.png" style="float: right" width="80%"/> |
− | <li>H410 Very toxic to aquatic life with long lasting effects.</li> | + | </div> |
− | </ul> | + | <br style="clear: both"> |
− | <p style="text-align:center"><strong> Precautionary statement(s)</strong></p> | + | <p style="text-align:center"><strong>Hazard statement(s)</strong></p> |
− | <ul> | + | <ul> |
− | <li>P273 Avoid release to the environment.</li> | + | <li>H302 Harmful if swallowed.</li> |
− | <li>P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.</li> | + | <li>H315 Causes skin irritation.</li> |
− | <li>P501 Dispose of contents/container to an approved waste disposal plant.</li> | + | <li>H319 Causes serious eye irritation.</li> |
− | </ul> | + | <li>H410 Very toxic to aquatic life with long lasting effects.</li> |
+ | </ul> | ||
+ | <p style="text-align:center"><strong>Precautionary statement(s)</strong></p> | ||
+ | <ul> | ||
+ | <li>P273 Avoid release to the environment.</li> | ||
+ | <li>P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes.Remove contact lenses, if present and easy to do. Continue rinsing.</li> | ||
+ | <li>P501 Dispose of contents/container to an approved waste disposal plant.</li> | ||
+ | </ul> | ||
+ | |||
<!-- agarose --> | <!-- agarose --> | ||
− | <h2>Agarose</h2> | + | |
− | <p>Use: DNA electrophoresis gel matrix</p> | + | <h2 id="Agarose">Agarose</h2> |
− | <p>Non-Hazardous</p> | + | <div class="div50"> |
− | <h2>L(+)-Arabinose</h2> | + | <p><u>Use:</u> DNA electrophoresis gel matrix</p> |
− | <p>Use: Induction of pBAD expression</p> | + | <p>Non-Hazardous</p> |
+ | </div> | ||
+ | <div class="div50"> | ||
+ | <img src="https://static.igem.org/mediawiki/2016/d/df/T--Oxford--Agarose.png" style="float: right" width="100%"/> | ||
+ | </div> | ||
+ | <br style="clear: both"> | ||
+ | <!-- Arabinose --> | ||
+ | |||
+ | <h2 style="clear: both" id="L-Arabinose">L(+)-Arabinose</h2> | ||
+ | <div class="div50" style="padding-top: 50px"> | ||
+ | <p><u>Use:</u> Induction of pBAD expression</p> | ||
<p>Non-Hazardous</p> | <p>Non-Hazardous</p> | ||
+ | </div> | ||
+ | <div class="div50"> | ||
+ | <img src="https://static.igem.org/mediawiki/2016/a/af/T--Oxford--Arabinose.png" style="float: right" width="80%"/> | ||
+ | </div> | ||
+ | <br style="clear: both"> | ||
+ | |||
<!-- Bathocuproinedisulfonic Acid Disodium salt --> | <!-- Bathocuproinedisulfonic Acid Disodium salt --> | ||
− | <h2>Bathocuproinedisulfonic Acid Disodium Salt</h2> | + | |
− | <p>Use: Chromophore in Cu+/ | + | <h2 style="clear: both" id="BCA-Salt">Bathocuproinedisulfonic Acid Disodium Salt</h2> |
+ | <div class="div50"> | ||
+ | <p><u>Use:</u> Chromophore in Cu<sup>+</sup>/Cu<sup>2+</sup> detection assay</p> | ||
<p>Non-Hazardous</p> | <p>Non-Hazardous</p> | ||
+ | </div> | ||
+ | <div class="div50"> | ||
+ | <img src= "https://static.igem.org/mediawiki/2016/6/64/T--Oxford--BCA.png" style="float: right" width="90%"> | ||
+ | </div> | ||
+ | <br style="clear: both"> | ||
+ | |||
<!-- Ascorbate --> | <!-- Ascorbate --> | ||
− | <h2>L(+)-Sodium Ascorbate</h2> | + | |
− | <p>Use: Reducing agent in Cu+/ | + | <h2 style="clear: both" id="Sodium-Ascorbate">L(+)-Sodium Ascorbate</h2> |
+ | <div class="div50" style="padding-top: 50px"> | ||
+ | <p><u>Use:</u> Reducing agent in Cu<sup>+</sup>/Cu<sup>2+</sup> detection assay</p> | ||
<p>Non-Hazardous</p> | <p>Non-Hazardous</p> | ||
+ | </div> | ||
+ | <div class="div50"> | ||
+ | <img src="https://static.igem.org/mediawiki/2016/4/47/T--Oxford--Ascorbate.png" style="float: right" width="90%"/> | ||
+ | </div> | ||
+ | <br style="clear: both"> | ||
+ | |||
<!-- Glutathione --> | <!-- Glutathione --> | ||
− | <h2>L-Glutathione</h2> | + | |
− | <p>Use: Reducing agent in Cu+/ | + | <h2 style="clear: both" id="L-Glutathione">L-Glutathione</h2> |
+ | <div class="div50"> | ||
+ | <p><u>Use:</u> Reducing agent in Cu<sup>+</sup>/Cu<sup>2+</sup> detection assay.</p> | ||
<p>Non-Hazardous</p> | <p>Non-Hazardous</p> | ||
+ | </div> | ||
+ | <div class="div50"> | ||
+ | <img src="https://static.igem.org/mediawiki/2016/d/dd/T--Oxford--Glutathione.png" style="float: right" width="80%"/> | ||
+ | </div> | ||
+ | <br style="clear: both"> | ||
+ | |||
<!-- Chloramphenicol --> | <!-- Chloramphenicol --> | ||
− | <h2>Chloramphenicol</h2> | + | |
− | <p>Use: Antibiotic and positive selection marker for all plasmids except pBAD</p> | + | <h2 style="clear: both" id="Chloramphenicol">Chloramphenicol</h2> |
− | <p>Signal word: Danger</p> | + | <div class="div50"> |
+ | <p><u>Use:</u> Antibiotic and positive selection marker for all plasmids except pBAD</p> | ||
+ | <p><u>Signal word:</u> Danger</p> | ||
+ | </div> | ||
+ | <div class="div50"> | ||
+ | <img src="https://static.igem.org/mediawiki/2016/c/cd/T--Oxford--Chloramphenicol.png" style="float: right" width="90%"/> | ||
+ | </div> | ||
+ | <br style="clear: both"> | ||
<p style="text-align:center"><strong>Hazard statement(s)</strong></p> | <p style="text-align:center"><strong>Hazard statement(s)</strong></p> | ||
<ul> | <ul> | ||
Line 97: | Line 207: | ||
<li>P308 + P313 If exposed or concerned: Get medical advice/attention.</li> | <li>P308 + P313 If exposed or concerned: Get medical advice/attention.</li> | ||
</ul> | </ul> | ||
+ | |||
<!-- Ampicillin --> | <!-- Ampicillin --> | ||
− | <h2>Ampicillin</h2> | + | |
− | <p>Use: Antibiotic and positive selection marker for pBAD.</p> | + | <h2 id="Ampicillin">Ampicillin</h2> |
− | <p>Signal word: Danger</p> | + | <div class="div50"> |
+ | <p><u>Use:</u> Antibiotic and positive selection marker for pBAD.</p> | ||
+ | <p><u>Signal word:</u> Danger</p> | ||
+ | </div> | ||
+ | <div class="div50"> | ||
+ | <img src="https://static.igem.org/mediawiki/2016/8/8f/T--Oxford--Ampicillin.png" style="float: right" width="90%"/> | ||
+ | </div> | ||
+ | <br style="clear: both"> | ||
<p style="text-align:center"><strong>Hazard statement(s)</strong></p> | <p style="text-align:center"><strong>Hazard statement(s)</strong></p> | ||
<ul> | <ul> | ||
Line 116: | Line 234: | ||
<li>P342 + P311 If experiencing respiratory symptoms: Call a POISON CENTER or doctor/physician</li> | <li>P342 + P311 If experiencing respiratory symptoms: Call a POISON CENTER or doctor/physician</li> | ||
</ul> | </ul> | ||
+ | |||
<!-- Tris --> | <!-- Tris --> | ||
− | <h2>Tris pH 7.0 and 8.0</h2> | + | |
− | <p>Use: General buffer for protein and copper assays</p> | + | <h2 id="Tris">Tris pH 7.0 and 8.0</h2> |
+ | <div class="div50" style="padding-top: 30px"> | ||
+ | <p><u>Use:</u> General buffer for protein and copper assays</p> | ||
<p>Non-Hazardous</p> | <p>Non-Hazardous</p> | ||
+ | </div> | ||
+ | <div class="div50"> | ||
+ | <img src="https://static.igem.org/mediawiki/2016/6/62/T--Oxford--TRIS.png" style="float: right" width="65%"/> | ||
+ | </div> | ||
+ | <br style="clear: both"> | ||
+ | |||
<!-- Glycerol --> | <!-- Glycerol --> | ||
− | <h2>Glycerol</h2> | + | |
− | <p>Use: Component of protein buffer for protein stability at low temperatures. Used in protein purification lysis and elution buffers. Also, used in SDS-PAGE run buffer</p> | + | <h2 style="clear: both" id="Glycerol">Glycerol</h2> |
+ | <div class="div50"> | ||
+ | <p><u>Use:</u> Component of protein buffer for protein stability at low temperatures. Used in protein purification lysis and elution buffers. Also, used in SDS-PAGE run buffer</p> | ||
<p>Non-Hazardous</p> | <p>Non-Hazardous</p> | ||
+ | </div> | ||
+ | <div class="div50"> | ||
+ | <img src="https://static.igem.org/mediawiki/2016/f/f6/T--Oxford--Glycerol.png" style="float: right" width="90%"/> | ||
+ | </div> | ||
+ | <br style="clear: both"> | ||
+ | |||
<!-- DTT --> | <!-- DTT --> | ||
− | <h2>DL-Dithiothreitol</h2> | + | |
− | <p>Use: Reducing agent in Cu+/ | + | <h2 style="clear: both" id="DL-Dithiothreitol">DL-Dithiothreitol</h2> |
− | <p>Signal word: Warning</p> | + | <div class="div50"> |
+ | <p><u>Use:</u> Reducing agent in Cu<sup>+</sup>/Cu<sup>2+</sup> detection assay and protein purification</p> | ||
+ | <p><u>Signal word:</u> Warning</p> | ||
+ | </div> | ||
+ | <div class="div50"> | ||
+ | <img src="https://static.igem.org/mediawiki/2016/e/ee/T--Oxford--DTT.png" style="float: right" width="90%"/> | ||
+ | </div> | ||
+ | <br style="clear: both"> | ||
<p style="text-align:center"><strong>Hazard statement(s)</strong></p> | <p style="text-align:center"><strong>Hazard statement(s)</strong></p> | ||
<ul> | <ul> | ||
Line 140: | Line 282: | ||
<li>P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing</li> | <li>P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing</li> | ||
</ul> | </ul> | ||
+ | |||
<!-- Imidiazole --> | <!-- Imidiazole --> | ||
− | <h2>Imidazole</h2> | + | |
− | <p>Use: Elution of His-tagged target proteins from nickel bead column during protein purification</p> | + | <h2 id="Imidiazole">Imidazole</h2> |
− | <p>Signal word: Danger</p> | + | <div class="div50" style="padding-top: 55px"> |
+ | <p><u>Use:</u> Elution of His-tagged target proteins from nickel bead column during protein purification</p> | ||
+ | <p><u>Signal word:</u> Danger</p> | ||
+ | </div> | ||
+ | <div class="div50"> | ||
+ | <img src="https://static.igem.org/mediawiki/2016/0/01/T--Oxford--Imidiazole.png" style="float: right" width="60%"/> | ||
+ | </div> | ||
+ | <br style="clear: both"> | ||
<p style="text-align:center"><strong>Hazard statement(s)</strong></p> | <p style="text-align:center"><strong>Hazard statement(s)</strong></p> | ||
<ul> | <ul> | ||
Line 157: | Line 307: | ||
<li>P310 Immediately call a POISON CENTER or doctor/physician</li> | <li>P310 Immediately call a POISON CENTER or doctor/physician</li> | ||
</ul> | </ul> | ||
+ | |||
<!-- Nickel affinity beads --> | <!-- Nickel affinity beads --> | ||
− | <h2>Nickel Affinity Beads</h2> | + | |
− | <p>Use: Selection of His-tagged target proteins during protein purification</p> | + | <h2 id="Nickel-Affinity-Beads">Nickel Affinity Beads</h2> |
+ | <div class="div50"> | ||
+ | <p><u>Use:</u> Selection of His-tagged target proteins during protein purification</p> | ||
<p>Non-Hazardous</p> | <p>Non-Hazardous</p> | ||
+ | </div> | ||
+ | <div class="div50"> | ||
+ | <img src="https://static.igem.org/mediawiki/2016/b/b9/T--Oxford--NiBEADS.gif" id=" Cont" style="float: right" width="90%"/> | ||
+ | </div> | ||
+ | <br id="Cont"> | ||
+ | <br style="clear: both"> | ||
+ | |||
<!-- Sodium Alginate --> | <!-- Sodium Alginate --> | ||
− | <h2>Sodium Alginate</h2> | + | |
− | <p>Use: Major component of bacterial delivery beads</p> | + | <h2 style="clear: both" id="Sodium-Alginate">Sodium Alginate</h2> |
+ | <div class="div50"> | ||
+ | <p><u>Use:</u> Major component of bacterial delivery beads</p> | ||
<p>Non-Hazardous</p> | <p>Non-Hazardous</p> | ||
+ | </div> | ||
+ | <div class="div50"> | ||
+ | <img src="https://static.igem.org/mediawiki/2016/8/8a/T--Oxford--ALGINATE.png" style="float: right" width="90%"/> | ||
+ | </div> | ||
+ | <br style="clear: both"> | ||
+ | |||
<!-- Crystal Violet --> | <!-- Crystal Violet --> | ||
− | <h2>Crystal Violet</h2> | + | |
− | <p>Use: Marker for bead quality assay</p> | + | <h2 style="clear: both" id="Crystal-Violet">Crystal Violet</h2> |
− | <p>Signal word: Danger</p> | + | <div class="div50"> |
+ | <p><u>Use:</u> Marker for bead quality assay</p> | ||
+ | <p><u>Signal word:</u> Danger</p> | ||
+ | </div> | ||
+ | <div class="div50"> | ||
+ | <img src="https://static.igem.org/mediawiki/2016/f/f6/T--Oxford--Crystal_Violet.png" style="float: right" width="70%"/> | ||
+ | </div> | ||
+ | <br style="clear: both"> | ||
<p style="text-align:center"><strong>Hazard statement(s)</strong></p> | <p style="text-align:center"><strong>Hazard statement(s)</strong></p> | ||
<ul> | <ul> | ||
Line 185: | Line 360: | ||
<li>P501 Dispose of contents/container to an approved waste disposal plant</li> | <li>P501 Dispose of contents/container to an approved waste disposal plant</li> | ||
</ul> | </ul> | ||
+ | |||
<!-- Lysogeny Broth --> | <!-- Lysogeny Broth --> | ||
− | <h2>Lysogeny Broth</h2> | + | |
− | <p>Use: | + | <h2 id="Lysogeny-Broth">Lysogeny Broth</h2> |
+ | <div class="div50" style="padding-top: 50px"> | ||
+ | <p><u>Use:</u> Medium for general bacterial growth</p> | ||
<p>Non-Hazardous</p> | <p>Non-Hazardous</p> | ||
+ | </div> | ||
+ | <div class="div50"> | ||
+ | <img src="https://static.igem.org/mediawiki/2016/a/ab/T--Oxford--LB.png" style="float: right" width="70%"/> | ||
+ | </div> | ||
+ | <br style="clear: both"> | ||
+ | |||
<!-- Phosphate buffered saline --> | <!-- Phosphate buffered saline --> | ||
− | <h2>Phosphate Buffered Saline pH 7.4</h2> | + | |
− | <p>Use: Dilution buffer for flow cytometry experiments</p> | + | <h2 style="clear: both" id="Phosphate-Buffered-Saline">Phosphate Buffered Saline pH 7.4</h2> |
+ | <div class="div50"> | ||
+ | <p><u>Use:</u> Dilution buffer for flow cytometry experiments</p> | ||
<p>Non-Hazardous</p> | <p>Non-Hazardous</p> | ||
+ | </div> | ||
+ | <div class="div50"> | ||
+ | <img src="https://static.igem.org/mediawiki/2016/c/c1/T--Oxford--PBS.gif " style="float: right" width="65%"/> | ||
+ | </div> | ||
+ | <br style="clear: both"> | ||
+ | |||
<!-- SDS --> | <!-- SDS --> | ||
− | <h2>Sodium Dodecyl Sulphate</h2> | + | |
− | <p>Use: Detergent in protein identification gels</p> | + | <h2 style="clear: both" id="Sodium-Dodecyl-Sulfate">Sodium Dodecyl Sulphate</h2> |
− | <p>Signal word: Danger</p> | + | |
+ | <div class="div50"> | ||
+ | <p><u>Use:</u> Detergent in protein identification gels</p> | ||
+ | <p><u>Signal word:</u> Danger</p> | ||
+ | </div> | ||
+ | <div class="div50"> | ||
+ | <img src="https://static.igem.org/mediawiki/2016/1/15/T--Oxford--SDS.jpg" style="float: right" width="90%"/> | ||
+ | </div> | ||
+ | <br style="clear: both"> | ||
<p style="text-align:center"><strong>Hazard statement(s)</strong></p> | <p style="text-align:center"><strong>Hazard statement(s)</strong></p> | ||
<ul> | <ul> | ||
Line 215: | Line 415: | ||
<li>P370 + P378 In case of fire: Use dry powder or dry sand to extinguish</li> | <li>P370 + P378 In case of fire: Use dry powder or dry sand to extinguish</li> | ||
</ul> | </ul> | ||
+ | |||
<!-- Ethanol --> | <!-- Ethanol --> | ||
− | <h2>Ethanol 100%</h2> | + | |
− | <p>Use: | + | <h2 id="Ethanol">Ethanol 100%</h2> |
− | <p>Signal word: Danger</p> | + | <div class="div50"> |
+ | <p><u>Use:</u> solvent for chloramphenicol</p> | ||
+ | <p><u>Signal word:</u> Danger</p> | ||
+ | </div> | ||
+ | <div class="div50"> | ||
+ | <img src="https://static.igem.org/mediawiki/2016/b/b2/T--Oxford--Ethanol.png" style="float: right; padding-top: 30px" width="60%"/> | ||
+ | </div> | ||
+ | <br style="clear: both"> | ||
<p style="text-align:center"><strong> Hazard statement(s)</strong></p> | <p style="text-align:center"><strong> Hazard statement(s)</strong></p> | ||
<ul> | <ul> | ||
Line 231: | Line 439: | ||
<li>P403 + P235 Store in a well-ventilated place. Keep cool</li> | <li>P403 + P235 Store in a well-ventilated place. Keep cool</li> | ||
</ul> | </ul> | ||
+ | |||
<!-- TFB I--> | <!-- TFB I--> | ||
− | <h2>Transfection Buffer I (Manganese[II] Chloride)</h2> | + | |
− | <p>Use: First soaking buffer in creating competent E. coli cells</p> | + | <h2 id="Transfection-Buffer-I">Transfection Buffer I (Manganese[II] Chloride)</h2> |
− | <p>Signal word: Danger</p> | + | <div class="div50"> |
+ | <p><u>Use:</u> First soaking buffer in creating competent E. coli cells</p> | ||
+ | <p><u>Signal word:</u> Danger</p> | ||
+ | </div> | ||
+ | <div class="div50"> | ||
+ | <img src="https://static.igem.org/mediawiki/2016/f/fa/T--Oxford--MnCl.png" style="float: right" width="60%"/> | ||
+ | </div> | ||
+ | <br style="clear: both"> | ||
<p style="text-align:center"><strong>Hazard statement(s)</strong></p> | <p style="text-align:center"><strong>Hazard statement(s)</strong></p> | ||
<ul> | <ul> | ||
Line 251: | Line 467: | ||
<li>P501 Dispose of contents/container to an approved waste disposal plant</li> | <li>P501 Dispose of contents/container to an approved waste disposal plant</li> | ||
</ul> | </ul> | ||
+ | |||
<!-- TFB II --> | <!-- TFB II --> | ||
− | <h2>Transfection Buffer II</h2> | + | |
− | <p>Use: Second soaking buffer in creating competent E. coli cells</p> | + | <h2 id="Transfection-Buffer-II">Transfection Buffer II</h2> |
+ | |||
+ | <p><u>Use:</u> Second soaking buffer in creating competent E. coli cells</p> | ||
<p>Non-Hazardous</p> | <p>Non-Hazardous</p> | ||
− | <h2>Chitosan</h2> | + | |
− | <p>Use: Coating for bacterial delivery beads</p> | + | <!--Chitosan--> |
+ | |||
+ | <h2 id="Chitosan">Chitosan</h2> | ||
+ | <div class="div50"> | ||
+ | <p><u>Use:</u> Coating for bacterial delivery beads</p> | ||
<p>Non-Hazardous</p> | <p>Non-Hazardous</p> | ||
− | <h2>InstantBlue</h2> | + | </div> |
− | <p>Use: Protein stain for protein identification gels</p> | + | <div class="div50"> |
− | <p>Signal word: Warning</p> | + | <img src="https://static.igem.org/mediawiki/2016/4/4a/T--Oxford--Chitosan.png" style="float: right" width="90%"/> |
+ | </div> | ||
+ | <br style="clear: both"> | ||
+ | |||
+ | <!-- Instant Blue --> | ||
+ | |||
+ | <h2 style="clear: both" id="Instant-Blue">InstantBlue</h2> | ||
+ | <div class="div50"> | ||
+ | <p><u>Use:</u> Protein stain for protein identification gels</p> | ||
+ | <p><u>Signal word:</u> Warning</p> | ||
+ | </div> | ||
+ | <div class="div50"> | ||
+ | <img src="https://static.igem.org/mediawiki/2016/9/9c/T--Oxford--InstantBlue.png" style="float: right" width="90%"/> | ||
+ | </div> | ||
+ | <br style="clear: both"> | ||
<p style="text-align:center"><strong> Hazard statement(s)</strong></p> | <p style="text-align:center"><strong> Hazard statement(s)</strong></p> | ||
<ul> | <ul> | ||
− | <li>H290 May be corrosive to metals</li> | + | <li>H290 May be corrosive to metals</li> |
− | <li>H315 Causes skin irritation</li> | + | <li>H315 Causes skin irritation</li> |
− | <li>H319 Causes serious eye irritation</li> | + | <li>H319 Causes serious eye irritation</li> |
</ul> | </ul> | ||
<p style="text-align:center"><strong> Precautionary statement(s)</strong></p> | <p style="text-align:center"><strong> Precautionary statement(s)</strong></p> | ||
Line 271: | Line 508: | ||
<li>P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing</li> | <li>P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing</li> | ||
</ul> | </ul> | ||
− | <!-- Purple | + | |
− | <h2>NEB Purple Loading Dye</h2> | + | <!-- Purple Loading Dye --> |
− | <p>Use: Stabilizer and marker for DNA gel electrophoresis</p> | + | |
+ | <h2 id="NEB-Purple-Loading-Dye">NEB Purple Loading Dye</h2> | ||
+ | <div class="div50"> | ||
+ | <p><u>Use:</u> Stabilizer and marker for DNA gel electrophoresis</p> | ||
<p>Non-Hazardous</p> | <p>Non-Hazardous</p> | ||
− | + | </div> | |
− | <h2>Roche cOmplete EDTA-free Protease Inhibitor Cocktail</h2> | + | <div class="div50"> |
− | <p>Use: Prevention of protein cleavage during protein purification</p> | + | <img src="https://static.igem.org/mediawiki/2016/8/88/T--Oxford--pURPLE_LOADING_DYE.jpg" style="float: right"/> |
− | <p>Signal word Danger</p> | + | </div> |
+ | <br style="clear: both"> | ||
+ | |||
+ | <!-- Protease Inhibitors --> | ||
+ | |||
+ | <h2 style="clear: both" id="Protease-Inhibitors">Roche cOmplete EDTA-free Protease Inhibitor Cocktail</h2> | ||
+ | <div class="div50"> | ||
+ | <p><u>Use:</u> Prevention of protein cleavage during protein purification</p> | ||
+ | <p><u>Signal word:</u> Danger</p> | ||
+ | </div> | ||
+ | <div class="div50"> | ||
+ | <img src="https://static.igem.org/mediawiki/2016/6/68/T--Oxford--protease_inhibitors.jpg" style="float: right"/> | ||
+ | </div> | ||
+ | <br style="clear: both"> | ||
<p style="text-align:center"><strong> Hazard statement(s)</strong></p> | <p style="text-align:center"><strong> Hazard statement(s)</strong></p> | ||
<ul> | <ul> | ||
Line 285: | Line 538: | ||
<p style="text-align:center"><strong> Precautionary statement(s)</strong></p> | <p style="text-align:center"><strong> Precautionary statement(s)</strong></p> | ||
<ul> | <ul> | ||
− | <li>P260 Do not breathe dust/fume/gas/mist/vapours/spray</li> | + | <li>P260 Do not breathe dust/fume/gas/mist/vapours/spray</li> |
− | <li>P280 Wear protective gloves/protective clothing/eye protection/face protection</li> | + | <li>P280 Wear protective gloves/protective clothing/eye protection/face protection</li> |
− | <li>P301 + P330 + P331 IF SWALLOWED: Rinse mouth. Do NOT induce vomiting</li> | + | <li>P301 + P330 + P331 IF SWALLOWED: Rinse mouth. Do NOT induce vomiting</li> |
− | <li>P303 + P361 + P353 IF ON SKIN (or hair): Remove/Take off Immediately all contaminated clothing. Rinse SKIN with water/shower</li> | + | <li>P303 + P361 + P353 IF ON SKIN (or hair): Remove/Take off Immediately all contaminated clothing. Rinse SKIN with water/shower</li> |
− | <li>P304 + P340 + P310 IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing. Immediately call a POISON CENTER or doctor/physician</li> | + | <li>P304 + P340 + P310 IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing. Immediately call a POISON CENTER or doctor/physician</li> |
− | <li>P305 + P351 + P338 + P310 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER or doctor/physician</li> | + | <li>P305 + P351 + P338 + P310 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER or doctor/physician</li> |
</ul> | </ul> | ||
+ | |||
<!-- HCL --> | <!-- HCL --> | ||
− | <h2>Hydrochloric Acid | + | <h2 style="clear: both" id="Hydrochloric-Acid">Hydrochloric Acid</h2> |
− | <p>Use: Simulation of stomach acid conditions in bead experiments</p> | + | <div class="div50"> |
− | <p>Signal word: Warning</p> | + | <p><u>Use:</u> Simulation of stomach acid conditions in bead experiments</p> |
+ | <p><u>Signal word:</u> Warning</p> | ||
+ | </div> | ||
+ | <div class="div50"> | ||
+ | <img src="https://static.igem.org/mediawiki/2016/f/f5/T--Oxford--HCL.png" style="float: right" width="90%"/> | ||
+ | </div> | ||
+ | <br style="clear: both"> | ||
<p style="text-align:center"><strong> Hazard statement(s)</strong></p> | <p style="text-align:center"><strong> Hazard statement(s)</strong></p> | ||
<ul> | <ul> | ||
<li>H290 May be corrosive to metals</li> | <li>H290 May be corrosive to metals</li> | ||
</ul> | </ul> | ||
+ | |||
<!-- NaOH --> | <!-- NaOH --> | ||
− | <h2>Sodium Hydroxide</h2> | + | |
− | <p>Use: Simulation of duodenum conditions in bead experiments</p> | + | <h2 style="clear: both" id="Sodium-Hydroxide">Sodium Hydroxide</h2> |
− | <p>Signal word: Danger</p> | + | <div class="div50"> |
+ | <p><u>Use:</u> Simulation of duodenum conditions in bead experiments</p> | ||
+ | <p><u>Signal word:</u> Danger</p> | ||
+ | </div> | ||
+ | <div class="div50"> | ||
+ | <img src="https://static.igem.org/mediawiki/2016/d/d7/T--Oxford--SODIUM_HYDROXIDE.png" style="float: right" width="90%"/> | ||
+ | </div> | ||
+ | <br style="clear: both"> | ||
<p style="text-align:center"><strong> Hazard statement(s)</strong></p> | <p style="text-align:center"><strong> Hazard statement(s)</strong></p> | ||
<ul> | <ul> | ||
− | <li>H290 May be corrosive to metals</li> | + | <li>H290 May be corrosive to metals</li> |
− | <li>H314 Causes severe skin burns and eye damage</li> | + | <li>H314 Causes severe skin burns and eye damage</li> |
</ul> | </ul> | ||
<p style="text-align:center"><strong> Precautionary statement(s)</strong></p> | <p style="text-align:center"><strong> Precautionary statement(s)</strong></p> | ||
<ul> | <ul> | ||
− | <li>P260 Do not breathe dust/fume/gas/mist/vapours/spray</li> | + | <li>P260 Do not breathe dust/fume/gas/mist/vapours/spray</li> |
− | <li>P280 Wear protective gloves/protective clothing/eye protection/face protection</li> | + | <li>P280 Wear protective gloves/protective clothing/eye protection/face protection</li> |
− | <li>P303 + P361 + P353 IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/shower</li> | + | <li>P303 + P361 + P353 IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/shower</li> |
− | <li>P304 + P340 + P310 IF INHALED: Remove person to fresh air and keep comfortable for breathing. Immediately call a POISON CENTER/doctor</li> | + | <li>P304 + P340 + P310 IF INHALED: Remove person to fresh air and keep comfortable for breathing. Immediately call a POISON CENTER/doctor</li> |
− | <li>P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing</li> | + | <li>P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing</li> |
</ul> | </ul> | ||
+ | |||
<!-- Monobasic Sodium Phopshate --> | <!-- Monobasic Sodium Phopshate --> | ||
− | <h2>Monobasic Sodium Phosphate</h2> | + | |
− | <p>Use: Simulation of duodenum conditions in bead experiments</p> | + | <h2 style="clear: both" id="Monobasic-Sodium-Phosphate">Monobasic Sodium Phosphate</h2> |
+ | <div class="div50"> | ||
+ | <p><u>Use:</u> Simulation of duodenum conditions in bead experiments</p> | ||
<p>Non-Hazardous</p> | <p>Non-Hazardous</p> | ||
+ | </div> | ||
+ | <div class="div50"> | ||
+ | <img src="https://static.igem.org/mediawiki/2016/b/ba/T--Oxford--MBNaP.png" style="float: right"/> | ||
+ | </div> | ||
+ | <br style="clear: both"> | ||
+ | |||
<!-- Glacial Acetic ACid --> | <!-- Glacial Acetic ACid --> | ||
− | <h2>Glacial Acetic Acid</h2> | + | |
− | <p>Use: Bead experiments</p> | + | <h2 style="clear: both" id="Glacial-Acetic-Acid">Glacial Acetic Acid</h2> |
− | <p>Signal word: Danger</p> | + | <div class="div50"> |
+ | <p><u>Use:</u> Bead experiments</p> | ||
+ | <p><u>Signal word:</u> Danger</p> | ||
+ | </div> | ||
+ | <div class="div50"> | ||
+ | <img src="https://static.igem.org/mediawiki/2016/1/16/T--Oxford--Acetic_Acid.png" style="float: right" width="80%"/> | ||
+ | </div> | ||
+ | <br style="clear: both"> | ||
<p style="text-align:center"><strong> Hazard statement(s)</strong></p> | <p style="text-align:center"><strong> Hazard statement(s)</strong></p> | ||
<ul> | <ul> | ||
Line 339: | Line 623: | ||
<li>P370 + P378 In case of fire: Use dry powder or dry sand to extinguish</li> | <li>P370 + P378 In case of fire: Use dry powder or dry sand to extinguish</li> | ||
</ul> | </ul> | ||
+ | |||
<!-- CaCl --> | <!-- CaCl --> | ||
− | <h2>Calcium Chloride</h2> | + | |
− | <p>Use: Forming beads from alginate. Ca2+ causes alginate phase change</p> | + | <h2 style="clear: both" id="Calcium-Chloride">Calcium Chloride</h2> |
− | <p>Signal word Warning</p> | + | <div class="div50"> |
+ | <p><u>Use:</u> Forming beads from alginate. Ca2+ causes alginate phase change</p> | ||
+ | <p><u>Signal word:</u> Warning</p> | ||
+ | </div> | ||
+ | <div class="div50"> | ||
+ | <img src="https://static.igem.org/mediawiki/2016/8/85/T--Oxford--CACL2.png" style="float: right"/> | ||
+ | </div> | ||
+ | <br style="clear: both"> | ||
<p style="text-align:center"><strong> Hazard statement(s)</strong></p> | <p style="text-align:center"><strong> Hazard statement(s)</strong></p> | ||
<ul> | <ul> | ||
Line 351: | Line 643: | ||
<li>P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing</li> | <li>P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing</li> | ||
</ul> | </ul> | ||
+ | </div> | ||
+ | </div> | ||
</body> | </body> | ||
</html> | </html> | ||
{{:Team:Oxford/footer}} | {{:Team:Oxford/footer}} |
Latest revision as of 21:36, 7 November 2016
A variety of different chemicals were used for the execution of the wet lab experiments. The following list contains all the major chemicals used in our copper chelation system and delivery mechanism studies. Hazardous or dangerous chemicals have also a list of precautionary and hazardous statements as defined by the Globally Harmonized System of Classification and Labelling of Chemicals (GHS)
Ethidium Bromide
Use: Staining DNA electrophoresis gels.
Signal word: Danger
Hazard statement(s)
- H302 Harmful if swallowed.
- H330 Fatal if inhaled.
- H341 Suspected of causing genetic defects.
Precautionary statement(s)
- P260 Do not breathe dust/fume/gas/mist/vapours/spray.
- P281 Use personal protective equipment as required.
- P284 Wear respiratory protection.
- P310 Immediately call a POISON CENTER or doctor/ physician.
TBE Buffer pH 8.3
Use: General buffer for DNA experiments.
Made of three chemicals: Tris, borate and EDTA
Non-Hazardous
Copper Sulphate Pentahydrate
Use: Induction of copper promoters and Cu2+ chelation assays.
Signal word: Warning
Hazard statement(s)
- H302 Harmful if swallowed.
- H315 Causes skin irritation.
- H319 Causes serious eye irritation.
- H410 Very toxic to aquatic life with long lasting effects.
Precautionary statement(s)
- P273 Avoid release to the environment.
- P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes.Remove contact lenses, if present and easy to do. Continue rinsing.
- P501 Dispose of contents/container to an approved waste disposal plant.
Agarose
Use: DNA electrophoresis gel matrix
Non-Hazardous
L(+)-Arabinose
Use: Induction of pBAD expression
Non-Hazardous
Bathocuproinedisulfonic Acid Disodium Salt
Use: Chromophore in Cu+/Cu2+ detection assay
Non-Hazardous
L(+)-Sodium Ascorbate
Use: Reducing agent in Cu+/Cu2+ detection assay
Non-Hazardous
L-Glutathione
Use: Reducing agent in Cu+/Cu2+ detection assay.
Non-Hazardous
Chloramphenicol
Use: Antibiotic and positive selection marker for all plasmids except pBAD
Signal word: Danger
Hazard statement(s)
- H350 May cause cancer
Precautionary statement(s)
- P201 Obtain special instructions before use.
- P308 + P313 If exposed or concerned: Get medical advice/attention.
Ampicillin
Use: Antibiotic and positive selection marker for pBAD.
Signal word: Danger
Hazard statement(s)
- H315 Causes skin irritation
- H317 May cause an allergic skin reaction
- H319 Causes serious eye irritation
- H334 May cause allergy or asthma symptoms or breathing difficulties if inhaled
- H335 May cause respiratory irritation
Precautionary statement(s)
- P261 Avoid breathing dust/fume/gas/mist/vapours/spray
- P280 Wear protective gloves
- P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
- P342 + P311 If experiencing respiratory symptoms: Call a POISON CENTER or doctor/physician
Tris pH 7.0 and 8.0
Use: General buffer for protein and copper assays
Non-Hazardous
Glycerol
Use: Component of protein buffer for protein stability at low temperatures. Used in protein purification lysis and elution buffers. Also, used in SDS-PAGE run buffer
Non-Hazardous
DL-Dithiothreitol
Use: Reducing agent in Cu+/Cu2+ detection assay and protein purification
Signal word: Warning
Hazard statement(s)
- H302 Harmful if swallowed
- H315 Causes skin irritation
- H319 Causes serious eye irritation
- H335 May cause respiratory irritation
Precautionary statement(s)
- P261 Avoid breathing dust
- P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
Imidazole
Use: Elution of His-tagged target proteins from nickel bead column during protein purification
Signal word: Danger
Hazard statement(s)
- H302 Harmful if swallowed
- H314 Causes severe skin burns and eye damage
- H360D May damage the unborn child
Precautionary statement(s)
- P201 Obtain special instructions before use
- P280 Wear protective gloves/protective clothing/eye protection/face protection
- P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
- P310 Immediately call a POISON CENTER or doctor/physician
Nickel Affinity Beads
Use: Selection of His-tagged target proteins during protein purification
Non-Hazardous
Sodium Alginate
Use: Major component of bacterial delivery beads
Non-Hazardous
Crystal Violet
Use: Marker for bead quality assay
Signal word: Danger
Hazard statement(s)
- H302 Harmful if swallowed
- H318 Causes serious eye damage
- H350 May cause cancer
- H410 Very toxic to aquatic life with long lasting effects
Precautionary statement(s)
- P201 Obtain special instructions before use
- P273 Avoid release to the environment
- P280 Wear protective gloves/ eye protection/ face protection
- P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
- P308 + P313 If exposed or concerned: Get medical advice/attention
- P501 Dispose of contents/container to an approved waste disposal plant
Lysogeny Broth
Use: Medium for general bacterial growth
Non-Hazardous
Phosphate Buffered Saline pH 7.4
Use: Dilution buffer for flow cytometry experiments
Non-Hazardous
Sodium Dodecyl Sulphate
Use: Detergent in protein identification gels
Signal word: Danger
Hazard statement(s)
- H228 Flammable solid
- H302 + H332 Harmful if swallowed or if inhaled
- H315 Causes skin irritation
- H318 Causes serious eye damage
- H335 May cause respiratory irritation
- H412 Harmful to aquatic life with long lasting effects
Precautionary statement(s)
- P210 Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking
- P261 Avoid breathing dust/fume/gas/mist/vapours/spray
- P280 Wear eye protection/face protection
- P301 + P312 + P330 IF SWALLOWED: Call a POISON CENTER/doctor if you feel unwell. Rinse mouth
- P305 + P351 + P338 + P310 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER/doctor
- P370 + P378 In case of fire: Use dry powder or dry sand to extinguish
Ethanol 100%
Use: solvent for chloramphenicol
Signal word: Danger
Hazard statement(s)
- H225 Highly flammable liquid and vapour
- H319 Causes serious eye irritation
Precautionary statement(s)
- P210 Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking
- P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
- P370 + P378 In case of fire: Use dry powder or dry sand to extinguish
- P403 + P235 Store in a well-ventilated place. Keep cool
Transfection Buffer I (Manganese[II] Chloride)
Use: First soaking buffer in creating competent E. coli cells
Signal word: Danger
Hazard statement(s)
- H302 Harmful if swallowed
- H318 Causes serious eye damage
- H373 May cause damage to organs (Brain) through prolonged or repeated exposure if inhaled
- H411 Toxic to aquatic life with long lasting effects
Precautionary statement(s)
- P273 Avoid release to the environment
- P280 Wear eye protection/ face protection
- P301 + P312 + P330 IF SWALLOWED: Call a POISON CENTER or doctor/physician if you feel unwell. Rinse mouth
- P305 + P351 + P338 + P310 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER or doctor/physician
- P391 Collect spillage
- P501 Dispose of contents/container to an approved waste disposal plant
Transfection Buffer II
Use: Second soaking buffer in creating competent E. coli cells
Non-Hazardous
Chitosan
Use: Coating for bacterial delivery beads
Non-Hazardous
InstantBlue
Use: Protein stain for protein identification gels
Signal word: Warning
Hazard statement(s)
- H290 May be corrosive to metals
- H315 Causes skin irritation
- H319 Causes serious eye irritation
Precautionary statement(s)
- P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
NEB Purple Loading Dye
Use: Stabilizer and marker for DNA gel electrophoresis
Non-Hazardous
Roche cOmplete EDTA-free Protease Inhibitor Cocktail
Use: Prevention of protein cleavage during protein purification
Signal word: Danger
Hazard statement(s)
- H314 Causes severe skin burns and eye damage Skin corrosion/irritation
Precautionary statement(s)
- P260 Do not breathe dust/fume/gas/mist/vapours/spray
- P280 Wear protective gloves/protective clothing/eye protection/face protection
- P301 + P330 + P331 IF SWALLOWED: Rinse mouth. Do NOT induce vomiting
- P303 + P361 + P353 IF ON SKIN (or hair): Remove/Take off Immediately all contaminated clothing. Rinse SKIN with water/shower
- P304 + P340 + P310 IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing. Immediately call a POISON CENTER or doctor/physician
- P305 + P351 + P338 + P310 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER or doctor/physician
Hydrochloric Acid
Use: Simulation of stomach acid conditions in bead experiments
Signal word: Warning
Hazard statement(s)
- H290 May be corrosive to metals
Sodium Hydroxide
Use: Simulation of duodenum conditions in bead experiments
Signal word: Danger
Hazard statement(s)
- H290 May be corrosive to metals
- H314 Causes severe skin burns and eye damage
Precautionary statement(s)
- P260 Do not breathe dust/fume/gas/mist/vapours/spray
- P280 Wear protective gloves/protective clothing/eye protection/face protection
- P303 + P361 + P353 IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/shower
- P304 + P340 + P310 IF INHALED: Remove person to fresh air and keep comfortable for breathing. Immediately call a POISON CENTER/doctor
- P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
Monobasic Sodium Phosphate
Use: Simulation of duodenum conditions in bead experiments
Non-Hazardous
Glacial Acetic Acid
Use: Bead experiments
Signal word: Danger
Hazard statement(s)
- H226 Flammable liquid and vapour
- H314 Causes severe skin burns and eye damage
Precautionary statement(s)
- P210 Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking
- P260 Do not breathe dust/fume/gas/mist/vapours/spray
- P280 Wear protective gloves/protective clothing/eye protection/face protection
- P303 + P361 + P353 IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/shower
- P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
- P370 + P378 In case of fire: Use dry powder or dry sand to extinguish
Calcium Chloride
Use: Forming beads from alginate. Ca2+ causes alginate phase change
Signal word: Warning
Hazard statement(s)
- H319 Causes serious eye irritation
Precautionary statement(s)
- P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing