Difference between revisions of "Team:FAFU-CHINA/Parts"

 
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= <p align="center" style="text-align:center"><span style="font-family:'Times New Roman';font-size:14.0000pt">Proof</span><span style="font-family:'Times New Roman';font-size:14.0000pt"></span></p> =
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= <span style="font-family:'Times New Roman'">Parts</span> =  
== <span style="font-family:'Times New Roman'">1.Introduction</span> ==
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<p style="margin-top:4.2000pt;margin-right:0.0000pt;margin-bottom:5.2500pt;margin-left:0.0000pt;padding:0pt 0pt 0pt 0pt"><span style="font-family:Tahoma;letter-spacing:0pt;font-size:9.5pt">We constructed various basic pars, composited parts and devices to finish our project. You can find the detailed information in Basic Parts and Compose Parts Wiki pages.</span><span style="font-family:Tahoma;font-size:9.5000pt"></span></p>
<p><span style="font-family:'Times New Roman';font-size:12.0000pt"> </span><span style="font-family:'Times New Roman';font-size:12.0000pt">In our project, we transformed the expression vector of </span><i><span style="font-family:'Times New Roman';font-size:12pt">Cry</span></i><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;and </span><i><span style="font-family:'Times New Roman';font-size:12pt">Cyt</span></i><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;toxins. Based on the toxin groups, we constructed six toxins express devices as </span><i><span style="font-family:'Times New Roman';font-size:12pt">Figure1</span></i><span style="font-family:'Times New Roman';font-size:12.0000pt">. To compare the toxicities, we also tested the toxicity of </span><i><span style="font-family:'Times New Roman';font-size:12pt">Bacillus thuringiensis</span></i><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;strain </span><i><span style="font-family:'Times New Roman';font-size:12pt">LLP229</span></i><span style="font-family:'Times New Roman';font-size:12.0000pt">. </span>[[File:T--FAFU-CHINA--proof2.png | 825px | thumb | center ]]<span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
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<p style="margin-left:0pt;text-indent:0pt;padding:0pt">[[File:T--FAFU-CHINA--parts.png | 825px | thumb | center ]]<span style="font-family:sans-serif;letter-spacing:0pt;font-size:9.5pt"></span></p>
== <span style="font-family:'Times New Roman'">2.Material, Condition and Method</span> ==
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<p align="center" style="margin-top:4.2000pt;margin-right:0.0000pt;margin-bottom:5.2500pt;margin-left:0.0000pt;padding:0pt 0pt 0pt 0pt;text-align:center"><span style="font-family:Tahoma;letter-spacing:0pt;font-size:9.5pt"><em>Fig 1: The Overview of 2016 FAFU-CHINA Parts</em></span><span style="font-family:Tahoma;font-size:9.5000pt"></span></p>
<p style="text-indent:36.0000pt"><span style="font-family:'Times New Roman';font-size:12.0000pt">The larvae of </span><i><span style="font-family:'Times New Roman';font-size:12pt">Ae. albopictus</span></i><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;are fed in the Key Laboratory of Biopesticide and Chemical Biology. The generation of </span><i><span style="font-family:'Times New Roman';font-size:12pt">Ae. albopictus </span></i><span style="font-family:'Times New Roman';font-size:12.0000pt">is 118. The </span><i><span style="font-family:'Times New Roman';font-size:12pt">LLP229</span></i><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;strain of </span><i><span style="font-family:'Times New Roman';font-size:12pt">Bacillus thuringiensis</span></i><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;is provided by Key Laboratory of Biopesticide and Chemical Biology.</span><span style="font-family:'Times New Roman';font-size:11.0000pt">&nbsp;The concentration of </span><i><span style="font-family:'Times New Roman';font-size:12pt">LLP229 </span></i><span style="font-family:'Times New Roman';font-size:12.0000pt">is listed in each table.</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
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<p style="text-indent:36.0000pt"><span style="font-family:'Times New Roman';font-size:12.0000pt">The CC530 and FA479 strains of </span><i><span style="font-family:'Times New Roman';font-size:12pt">Chlamydomonas reinhardtii </span></i><span style="font-family:'Times New Roman';font-size:12.0000pt">and TAP medium are bought from the Aquatic Species Research Institute in Wuhan, Hubei.</span><span style="font-family:'Times New Roman';font-size:11.0000pt">&nbsp;The concentration of </span><i><span style="font-family:'Times New Roman';font-size:12pt">Chlamydomonas reinhardtii </span></i><span style="font-family:'Times New Roman';font-size:12.0000pt">is recorded in each table.</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
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<p style="text-indent:36.0000pt"><span style="font-family:'Times New Roman';font-size:12.0000pt">We contrasted various toxins group expression vectors and transform them into </span><i><span style="font-family:'Times New Roman';font-size:12pt">Chlamydomonas reinhardtii </span></i><span style="font-family:'Times New Roman';font-size:12.0000pt">as following groups: </span><i><span style="font-family:'Times New Roman';font-size:12pt">Cry4Aa+2A+Cyt1, Cry4Aa+2A+Cyt2, Cry10Aa+2A+Cyt1, Cry10Aa+2A+Cyt2, Cry11Aa+2A+Cyt1, Cry11Aa+2A+Cyt2</span></i><span style="font-family:'Times New Roman';font-size:12.0000pt">.</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
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<p style="text-indent:36.0000pt"><span style="font-family:'Times New Roman';font-size:12.0000pt">The toxicity analysis condition is in an environmentally controlled room (</span><i><span style="font-family:'Times New Roman'">T</span></i><span style="font-family:'Times New Roman';font-size:11.0000pt">=28±1&nbsp;°C, RH=85%±5%, L: D=14:10).</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
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<p style="text-indent:36.0000pt"><span style="font-family:'Times New Roman';font-size:12.0000pt">Bioassay of various concentrations was assayed against 30 third-instar larvae in 20 ml of dechlorinated water. Each group was replicated 3 times. The level of mortality was recorded after 48 hours. Then calculated the corrected mortality. The bioassay was then repeated with such that a concentration range yielding 10–90% mortality to obtain and the 50% lethal concentration (LC</span><sub><span style="font-family:'Times New Roman';font-size:12.0000pt;vertical-align:sub">50</span></sub><span style="font-family:'Times New Roman';font-size:12.0000pt">) was calculated.</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
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<p align="center" style="text-indent:36.0000pt;page-break-after:avoid;text-align:center"><span style="font-family:'Times New Roman';font-size:12pt">Table </span><span style="font-family:'Times New Roman';font-size:12pt">1</span><span style="font-family:'Times New Roman';font-size:12pt">.1 Toxicity of</span><i><span style="font-family:'Times New Roman';font-size:12pt">&nbsp;LLP29 </span></i><span style="font-family:'Times New Roman';font-size:12pt">to</span><i><span style="font-family:'Times New Roman';font-size:12pt">&nbsp;A. albopictus</span></i><i><span style="font-family:'Times New Roman';font-size:12pt"></span></i></p>
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<table style="border-collapse:collapse;width:471.0000pt;margin-left:6.7500pt;margin-right:6.7500pt">
 
<tr style="height:21.3000pt"><td width="133" valign="center" style="width:100.1000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:1.0000pt solid windowtext;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">Repeating group</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="145" valign="center" style="width:109.4000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:1.0000pt solid windowtext;border-bottom:1.0000pt solid windowtext"><p style="margin-bottom:0.0000pt"><span style="font-family:'Times New Roman';font-size:12.0000pt">Number of insects</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="162" valign="center" style="width:121.9000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:1.0000pt solid windowtext;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">Number of mortality</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="186" valign="center" style="width:139.6000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:1.0000pt solid windowtext;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">Corrected mortality (%)</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td></tr><tr style="height:21.3000pt"><td width="133" valign="center" style="width:100.1000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:none"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">1</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="145" valign="center" style="width:109.4000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:none"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">30</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="162" valign="center" style="width:121.9000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:none"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">30</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="186" valign="center" style="width:139.6000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:none"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">100</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td></tr><tr style="height:13.8500pt"><td width="133" valign="center" style="width:100.1000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">2</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="145" valign="center" style="width:109.4000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">30</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="162" valign="center" style="width:121.9000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">30</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="186" valign="center" style="width:139.6000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">100</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td></tr><tr style="height:13.8500pt"><td width="133" valign="center" style="width:100.1000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">3</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="145" valign="center" style="width:109.4000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">30</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="162" valign="center" style="width:121.9000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">30</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="186" valign="center" style="width:139.6000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">100</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td></tr><tr style="height:14.4000pt"><td width="133" valign="center" style="width:100.1000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">Control</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="145" valign="center" style="width:109.4000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">30</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="162" valign="center" style="width:121.9000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">0</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="186" valign="center" style="width:139.6000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">0</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td></tr>
 
</table><br>
 
<p align="center" style="text-align:center"><i><span style="font-family:'Times New Roman';font-size:12pt">Note.</span></i><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;Then concentration of </span><i><span style="font-family:'Times New Roman';font-size:12pt">LLP29 </span></i><span style="font-family:'Times New Roman';font-size:12.0000pt">is </span><span style="font-family:'Times New Roman';font-size:12.0000pt">2.16 x 10</span><sup><span style="font-family:'Times New Roman';font-size:12.0000pt;vertical-align:super">9</span></sup><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;(</span><span style="font-family:'Times New Roman';font-size:12.0000pt">OD</span><sub><span style="font-family:'Times New Roman';font-size:12.0000pt;vertical-align:sub">600</span></sub><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span><span style="font-family:'Times New Roman';font-size:12.0000pt">of 1.0 = 8 x 10</span><sup><span style="font-family:'Times New Roman';font-size:12.0000pt;vertical-align:super">8</span></sup><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span><span style="font-family:'Times New Roman';font-size:12.0000pt">cells/ml, OD</span><sub><span style="font-family:'Times New Roman';font-size:12.0000pt;vertical-align:sub">600 </span></sub><span style="font-family:'Times New Roman';font-size:12.0000pt">=2.7A.)</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
<p align="center" style="text-align:center"><span style="font-family:'Times New Roman';font-size:11.0000pt">&nbsp;</span></p>
 
<p align="center" style="text-indent:36.0000pt;page-break-after:avoid;text-align:center"><span style="font-family:'Times New Roman';font-size:12pt">Table </span><span style="font-family:'Times New Roman';font-size:12pt">1</span><span style="font-family:'Times New Roman';font-size:12pt">.2 Toxicity of</span><i><span style="font-family:'Times New Roman';font-size:12pt">&nbsp;LLP29 </span></i><span style="font-family:'Times New Roman';font-size:12pt">to</span><i><span style="font-family:'Times New Roman';font-size:12pt">&nbsp;Culex quinquefasciatus</span></i><i><span style="font-family:'Times New Roman';font-size:12pt"></span></i></p>
 
 
<table style="border-collapse:collapse;width:472.0000pt;margin-left:6.7500pt;margin-right:6.7500pt">
 
<tr style="height:23.0000pt"><td width="133" valign="center" style="width:100.3500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:1.0000pt solid windowtext;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">Repeating group</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="146" valign="center" style="width:109.6000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:1.0000pt solid windowtext;border-bottom:1.0000pt solid windowtext"><p style="margin-bottom:0.0000pt"><span style="font-family:'Times New Roman';font-size:12.0000pt">Number of insects</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="162" valign="center" style="width:122.1500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:1.0000pt solid windowtext;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">Number of mortality</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="186" valign="center" style="width:139.9000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:1.0000pt solid windowtext;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">Corrected mortality (%)</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td></tr><tr style="height:23.0000pt"><td width="133" valign="center" style="width:100.3500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:none"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">1</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="146" valign="center" style="width:109.6000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:none"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">30</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="162" valign="center" style="width:122.1500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:none"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">30</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="186" valign="center" style="width:139.9000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:none"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">100</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td></tr><tr style="height:14.9500pt"><td width="133" valign="center" style="width:100.3500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">2</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="146" valign="center" style="width:109.6000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">30</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="162" valign="center" style="width:122.1500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">30</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="186" valign="center" style="width:139.9000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">100</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td></tr><tr style="height:14.9500pt"><td width="133" valign="center" style="width:100.3500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">3</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="146" valign="center" style="width:109.6000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">30</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="162" valign="center" style="width:122.1500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">30</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="186" valign="center" style="width:139.9000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">100</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td></tr><tr style="height:15.5500pt"><td width="133" valign="center" style="width:100.3500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">Control</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="146" valign="center" style="width:109.6000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">30</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="162" valign="center" style="width:122.1500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">0</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="186" valign="center" style="width:139.9000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">0</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td></tr>
 
</table><br>
 
<p align="center" style="text-align:center"><i><span style="font-family:'Times New Roman';font-size:12pt">Note.</span></i><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;Then concentration of </span><i><span style="font-family:'Times New Roman';font-size:12pt">LLP29 </span></i><span style="font-family:'Times New Roman';font-size:12.0000pt">is </span><span style="font-family:'Times New Roman';font-size:12.0000pt">2.16 x 10</span><sup><span style="font-family:'Times New Roman';font-size:12.0000pt;vertical-align:super">9</span></sup><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;(</span><span style="font-family:'Times New Roman';font-size:12.0000pt">OD</span><sub><span style="font-family:'Times New Roman';font-size:12.0000pt;vertical-align:sub">600</span></sub><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span><span style="font-family:'Times New Roman';font-size:12.0000pt">of 1.0 = 8 x 10</span><sup><span style="font-family:'Times New Roman';font-size:12.0000pt;vertical-align:super">8</span></sup><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span><span style="font-family:'Times New Roman';font-size:12.0000pt">cells/ml, OD</span><sub><span style="font-family:'Times New Roman';font-size:12.0000pt;vertical-align:sub">600 </span></sub><span style="font-family:'Times New Roman';font-size:12.0000pt">=2.7A)</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
<p align="center" style="text-align:center"><span style="font-family:'Times New Roman';font-size:11.0000pt">&nbsp;</span></p>
 
<p align="center" style="text-indent:36.0000pt;page-break-after:avoid;text-align:center"><span style="font-family:'Times New Roman';font-size:12pt">Table 1.3 Toxicity of</span><i><span style="font-family:'Times New Roman';font-size:12pt">&nbsp;LLP29 </span></i><span style="font-family:'Times New Roman';font-size:12pt">to</span><i><span style="font-family:'Times New Roman';font-size:12pt">&nbsp;Culex quinquefasciatus</span></i><i><span style="font-family:'Times New Roman';font-size:12pt"></span></i></p>
 
 
<table style="border-collapse:collapse;width:474.0000pt;margin-left:6.7500pt;margin-right:6.7500pt">
 
<tr style="height:21.7000pt"><td width="134" valign="center" style="width:100.7500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:1.0000pt solid windowtext;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">Repeating group</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="146" valign="center" style="width:110.0500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:1.0000pt solid windowtext;border-bottom:1.0000pt solid windowtext"><p style="margin-bottom:0.0000pt"><span style="font-family:'Times New Roman';font-size:12.0000pt">Number of insects</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="163" valign="center" style="width:122.7500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:1.0000pt solid windowtext;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">Number of mortality</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="187" valign="center" style="width:140.4500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:1.0000pt solid windowtext;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">Corrected mortality (%)</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td></tr><tr style="height:21.7000pt"><td width="134" valign="center" style="width:100.7500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:none"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">1</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="146" valign="center" style="width:110.0500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:none"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">30</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="163" valign="center" style="width:122.7500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:none"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">30</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="187" valign="center" style="width:140.4500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:none"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">100</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td></tr><tr style="height:14.1000pt"><td width="134" valign="center" style="width:100.7500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">2</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="146" valign="center" style="width:110.0500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">30</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="163" valign="center" style="width:122.7500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">30</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="187" valign="center" style="width:140.4500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">100</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td></tr><tr style="height:14.1000pt"><td width="134" valign="center" style="width:100.7500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">3</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="146" valign="center" style="width:110.0500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">30</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="163" valign="center" style="width:122.7500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">30</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="187" valign="center" style="width:140.4500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">100</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td></tr><tr style="height:14.6500pt"><td width="134" valign="center" style="width:100.7500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">Control</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="146" valign="center" style="width:110.0500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">30</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="163" valign="center" style="width:122.7500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">0</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="187" valign="center" style="width:140.4500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">0</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td></tr>
 
</table><br>
 
<p align="center" style="text-align:center"><i><span style="font-family:'Times New Roman';font-size:12pt">Note.</span></i><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;Then concentration of </span><i><span style="font-family:'Times New Roman';font-size:12pt">LLP29 </span></i><span style="font-family:'Times New Roman';font-size:12.0000pt">is </span><span style="font-family:'Times New Roman';font-size:12.0000pt">2.16 x 10</span><sup><span style="font-family:'Times New Roman';font-size:12.0000pt;vertical-align:super">9</span></sup><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;(</span><span style="font-family:'Times New Roman';font-size:12.0000pt">OD</span><sub><span style="font-family:'Times New Roman';font-size:12.0000pt;vertical-align:sub">600</span></sub><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span><span style="font-family:'Times New Roman';font-size:12.0000pt">of 1.0 = 8 x 10</span><sup><span style="font-family:'Times New Roman';font-size:12.0000pt;vertical-align:super">8</span></sup><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span><span style="font-family:'Times New Roman';font-size:12.0000pt">cells/ml, OD</span><sub><span style="font-family:'Times New Roman';font-size:12.0000pt;vertical-align:sub">600 </span></sub><span style="font-family:'Times New Roman';font-size:12.0000pt">=2.7A)</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
<p><span style="font-family:'Times New Roman';font-size:11.0000pt">&nbsp;</span></p>
 
<p align="center" style="text-indent:36.0000pt;page-break-after:avoid;text-align:center"><span style="font-family:'Times New Roman';font-size:12pt">Table 2.1 Toxicity of </span><i><span style="font-family:'Times New Roman';font-size:12pt">Chlamydomonas reinhardtii </span></i><span style="font-family:'Times New Roman';font-size:12pt">(</span><i><span style="font-family:'Times New Roman';font-size:12pt">Cry4Aa+2A+Cyt1</span></i><span style="font-family:'Times New Roman';font-size:12pt">)</span><i><span style="font-family:'Times New Roman';font-size:12pt">&nbsp;</span></i><span style="font-family:'Times New Roman';font-size:12pt">to</span><i><span style="font-family:'Times New Roman';font-size:12pt">&nbsp;A. albopictus</span></i><i><span style="font-family:'Times New Roman';font-size:12pt"></span></i></p>
 
 
<table style="border-collapse:collapse;width:476.0000pt;margin-left:6.7500pt;margin-right:6.7500pt">
 
<tr style="height:22.2500pt"><td width="134" valign="center" style="width:101.1500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:1.0000pt solid windowtext;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">Repeating group</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="147" valign="center" style="width:110.5500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:1.0000pt solid windowtext;border-bottom:1.0000pt solid windowtext"><p style="margin-bottom:0.0000pt"><span style="font-family:'Times New Roman';font-size:12.0000pt">Number of insects</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="164" valign="center" style="width:123.2500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:1.0000pt solid windowtext;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">Number of mortality</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="188" valign="center" style="width:141.0500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:1.0000pt solid windowtext;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">Corrected mortality (%)</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td></tr><tr style="height:22.2500pt"><td width="134" valign="center" style="width:101.1500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:none"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">1</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="147" valign="center" style="width:110.5500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:none"><p style="margin-bottom:0.0000pt"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td><td width="164" valign="center" style="width:123.2500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:none"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td><td width="188" valign="center" style="width:141.0500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:none"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td></tr><tr style="height:14.1500pt"><td width="134" valign="center" style="width:101.1500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">2</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="147" valign="center" style="width:110.5500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td><td width="164" valign="center" style="width:123.2500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td><td width="188" valign="center" style="width:141.0500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td></tr><tr style="height:14.1500pt"><td width="134" valign="center" style="width:101.1500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">3</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="147" valign="center" style="width:110.5500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td><td width="164" valign="center" style="width:123.2500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td><td width="188" valign="center" style="width:141.0500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td></tr><tr style="height:29.4000pt"><td width="134" valign="center" style="width:101.1500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">Control 1</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
<p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">Control 2</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="147" valign="center" style="width:110.5500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td><td width="164" valign="center" style="width:123.2500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td><td width="188" valign="center" style="width:141.0500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td></tr>
 
</table><br>
 
<p><span style="font-family:'Times New Roman';font-size:12.0000pt">Note.1. Then concentration of </span><i><span style="font-family:'Times New Roman';font-size:12pt">Chlamydomonas reinhardtii </span></i><span style="font-family:'Times New Roman';font-size:12.0000pt">is xxx</span><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span><span style="font-family:'Times New Roman';font-size:12.0000pt">(</span><span style="font-family:'Times New Roman';font-size:12.0000pt">OD</span><sub><span style="font-family:'Times New Roman';font-size:12.0000pt;vertical-align:sub">600</span></sub><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span><span style="font-family:'Times New Roman';font-size:12.0000pt">of 1.0 = 8 x 10</span><sup><span style="font-family:'Times New Roman';font-size:12.0000pt;vertical-align:super">8</span></sup><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span><span style="font-family:'Times New Roman';font-size:12.0000pt">cells/ml, OD</span><sub><span style="font-family:'Times New Roman';font-size:12.0000pt;vertical-align:sub">600 </span></sub><span style="font-family:'Times New Roman';font-size:12.0000pt">=xxxx)</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
<p><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2. The Control 1 group is </span><i><span style="font-family:'Times New Roman';font-size:12pt">Chlamydomonas reinhardtii</span></i><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;(no transformation,</span><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;OD=?</span><span style="font-family:'Times New Roman';font-size:12.0000pt">)</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
<p><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3. The Control 2 group is TAP medium (Concentration =?)</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
<p><span style="font-family:'Times New Roman';font-size:11.0000pt">&nbsp;</span></p>
 
<p align="center" style="text-indent:36.0000pt;page-break-after:avoid;text-align:center"><span style="font-family:'Times New Roman';font-size:12pt">Table 2.2 Toxicity of </span><i><span style="font-family:'Times New Roman';font-size:12pt">Chlamydomonas reinhardtii </span></i><span style="font-family:'Times New Roman';font-size:12pt">(</span><i><span style="font-family:'Times New Roman';font-size:12pt">Cry4Aa+2A+Cyt2</span></i><span style="font-family:'Times New Roman';font-size:12pt">)</span><i><span style="font-family:'Times New Roman';font-size:12pt">&nbsp;</span></i><span style="font-family:'Times New Roman';font-size:12pt">to</span><i><span style="font-family:'Times New Roman';font-size:12pt">&nbsp;A. albopictus</span></i><i><span style="font-family:'Times New Roman';font-size:12pt"></span></i></p>
 
 
<table style="border-collapse:collapse;width:477.0000pt;margin-left:6.7500pt;margin-right:6.7500pt">
 
<tr style="height:20.8500pt"><td width="135" valign="center" style="width:101.4000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:1.0000pt solid windowtext;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">Repeating group</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="147" valign="center" style="width:110.7500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:1.0000pt solid windowtext;border-bottom:1.0000pt solid windowtext"><p style="margin-bottom:0.0000pt"><span style="font-family:'Times New Roman';font-size:12.0000pt">Number of insects</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="164" valign="center" style="width:123.5000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:1.0000pt solid windowtext;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">Number of mortality</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="188" valign="center" style="width:141.3500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:1.0000pt solid windowtext;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">Corrected mortality (%)</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td></tr><tr style="height:20.8500pt"><td width="135" valign="center" style="width:101.4000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:none"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">1</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="147" valign="center" style="width:110.7500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:none"><p style="margin-bottom:0.0000pt"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td><td width="164" valign="center" style="width:123.5000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:none"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td><td width="188" valign="center" style="width:141.3500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:none"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td></tr><tr style="height:13.5500pt"><td width="135" valign="center" style="width:101.4000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">2</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="147" valign="center" style="width:110.7500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td><td width="164" valign="center" style="width:123.5000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td><td width="188" valign="center" style="width:141.3500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td></tr><tr style="height:13.5500pt"><td width="135" valign="center" style="width:101.4000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">3</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="147" valign="center" style="width:110.7500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td><td width="164" valign="center" style="width:123.5000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td><td width="188" valign="center" style="width:141.3500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td></tr><tr style="height:27.7000pt"><td width="135" valign="center" style="width:101.4000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">Control 1</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
<p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">Control 2</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="147" valign="center" style="width:110.7500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td><td width="164" valign="center" style="width:123.5000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td><td width="188" valign="center" style="width:141.3500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td></tr>
 
</table><br>
 
<p><span style="font-family:'Times New Roman';font-size:12.0000pt">Note.1. Then concentration of </span><i><span style="font-family:'Times New Roman';font-size:12pt">Chlamydomonas reinhardtii </span></i><span style="font-family:'Times New Roman';font-size:12.0000pt">is xxx</span><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span><span style="font-family:'Times New Roman';font-size:12.0000pt">(</span><span style="font-family:'Times New Roman';font-size:12.0000pt">OD</span><sub><span style="font-family:'Times New Roman';font-size:12.0000pt;vertical-align:sub">600</span></sub><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span><span style="font-family:'Times New Roman';font-size:12.0000pt">of 1.0 = 8 x 10</span><sup><span style="font-family:'Times New Roman';font-size:12.0000pt;vertical-align:super">8</span></sup><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span><span style="font-family:'Times New Roman';font-size:12.0000pt">cells/ml, OD</span><sub><span style="font-family:'Times New Roman';font-size:12.0000pt;vertical-align:sub">600 </span></sub><span style="font-family:'Times New Roman';font-size:12.0000pt">=xxxx)</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
<p><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2. The Control 1 group is </span><i><span style="font-family:'Times New Roman';font-size:12pt">Chlamydomonas reinhardtii</span></i><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;(no transformation,</span><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;OD=?</span><span style="font-family:'Times New Roman';font-size:12.0000pt">)</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
<p><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3. The Control 2 group is TAP medium (Concentration =?)</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
<p><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
<p align="center" style="text-indent:36.0000pt;page-break-after:avoid;text-align:center"><span style="font-family:'Times New Roman';font-size:12pt">Table 2.3 Toxicity of </span><i><span style="font-family:'Times New Roman';font-size:12pt">Chlamydomonas reinhardtii </span></i><span style="font-family:'Times New Roman';font-size:12pt">(</span><i><span style="font-family:'Times New Roman';font-size:12pt">Cry10Aa+2A+Cyt1</span></i><span style="font-family:'Times New Roman';font-size:12pt">)</span><i><span style="font-family:'Times New Roman';font-size:12pt">&nbsp;</span></i><span style="font-family:'Times New Roman';font-size:12pt">to</span><i><span style="font-family:'Times New Roman';font-size:12pt">&nbsp;A. albopictus</span></i><i><span style="font-family:'Times New Roman';font-size:12pt"></span></i></p>
 
 
<table style="border-collapse:collapse;width:481.0000pt;margin-left:6.7500pt;margin-right:6.7500pt">
 
<tr style="height:20.6500pt"><td width="136" valign="center" style="width:102.2500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:1.0000pt solid windowtext;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">Repeating group</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="148" valign="center" style="width:111.7000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:1.0000pt solid windowtext;border-bottom:1.0000pt solid windowtext"><p style="margin-bottom:0.0000pt"><span style="font-family:'Times New Roman';font-size:12.0000pt">Number of insects</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="166" valign="center" style="width:124.5000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:1.0000pt solid windowtext;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">Number of mortality</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="190" valign="center" style="width:142.5500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:1.0000pt solid windowtext;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">Corrected mortality (%)</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td></tr><tr style="height:20.6500pt"><td width="136" valign="center" style="width:102.2500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:none"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">1</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="148" valign="center" style="width:111.7000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:none"><p style="margin-bottom:0.0000pt"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td><td width="166" valign="center" style="width:124.5000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:none"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td><td width="190" valign="center" style="width:142.5500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:none"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td></tr><tr><td width="136" valign="center" style="width:102.2500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">2</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="148" valign="center" style="width:111.7000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td><td width="166" valign="center" style="width:124.5000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td><td width="190" valign="center" style="width:142.5500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td></tr><tr><td width="136" valign="center" style="width:102.2500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">3</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="148" valign="center" style="width:111.7000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td><td width="166" valign="center" style="width:124.5000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td><td width="190" valign="center" style="width:142.5500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td></tr><tr><td width="136" valign="center" style="width:102.2500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">Control 1</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
<p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">Control 2</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="148" valign="center" style="width:111.7000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td><td width="166" valign="center" style="width:124.5000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td><td width="190" valign="center" style="width:142.5500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td></tr>
 
</table><br>
 
<p><span style="font-family:'Times New Roman';font-size:12.0000pt">Note.1. Then concentration of </span><i><span style="font-family:'Times New Roman';font-size:12pt">Chlamydomonas reinhardtii </span></i><span style="font-family:'Times New Roman';font-size:12.0000pt">is xxx</span><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span><span style="font-family:'Times New Roman';font-size:12.0000pt">(</span><span style="font-family:'Times New Roman';font-size:12.0000pt">OD</span><sub><span style="font-family:'Times New Roman';font-size:12.0000pt;vertical-align:sub">600</span></sub><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span><span style="font-family:'Times New Roman';font-size:12.0000pt">of 1.0 = 8 x 10</span><sup><span style="font-family:'Times New Roman';font-size:12.0000pt;vertical-align:super">8</span></sup><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span><span style="font-family:'Times New Roman';font-size:12.0000pt">cells/ml, OD</span><sub><span style="font-family:'Times New Roman';font-size:12.0000pt;vertical-align:sub">600 </span></sub><span style="font-family:'Times New Roman';font-size:12.0000pt">=xxxx)</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
<p><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2. The Control 1 group is </span><i><span style="font-family:'Times New Roman';font-size:12pt">Chlamydomonas reinhardtii</span></i><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;(no transformation,</span><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;OD=?</span><span style="font-family:'Times New Roman';font-size:12.0000pt">)</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
<p><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3. The Control 2 group is TAP medium (Concentration =?)</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
<p><span style="font-family:'Times New Roman';font-size:11.0000pt">&nbsp;</span></p>
 
<p align="center" style="text-indent:36.0000pt;page-break-after:avoid;text-align:center"><span style="font-family:'Times New Roman';font-size:12pt">Table 2.4 Toxicity of </span><i><span style="font-family:'Times New Roman';font-size:12pt">Chlamydomonas reinhardtii </span></i><span style="font-family:'Times New Roman';font-size:12pt">(</span><i><span style="font-family:'Times New Roman';font-size:12pt">Cry10Aa+2A+Cyt2</span></i><span style="font-family:'Times New Roman';font-size:12pt">)</span><i><span style="font-family:'Times New Roman';font-size:12pt">&nbsp;</span></i><span style="font-family:'Times New Roman';font-size:12pt">to</span><i><span style="font-family:'Times New Roman';font-size:12pt">&nbsp;A. albopictus</span></i><i><span style="font-family:'Times New Roman';font-size:12pt"></span></i></p>
 
 
<table style="border-collapse:collapse;width:484.0000pt;margin-left:6.7500pt;margin-right:6.7500pt">
 
<tr style="height:21.4500pt"><td width="137" valign="center" style="width:102.9000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:1.0000pt solid windowtext;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">Repeating group</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="149" valign="center" style="width:112.3500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:1.0000pt solid windowtext;border-bottom:1.0000pt solid windowtext"><p style="margin-bottom:0.0000pt"><span style="font-family:'Times New Roman';font-size:12.0000pt">Number of insects</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="167" valign="center" style="width:125.3000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:1.0000pt solid windowtext;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">Number of mortality</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="191" valign="center" style="width:143.4500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:1.0000pt solid windowtext;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">Corrected mortality (%)</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td></tr><tr style="height:21.4500pt"><td width="137" valign="center" style="width:102.9000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:none"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">1</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="149" valign="center" style="width:112.3500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:none"><p style="margin-bottom:0.0000pt"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td><td width="167" valign="center" style="width:125.3000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:none"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td><td width="191" valign="center" style="width:143.4500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:none"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td></tr><tr style="height:13.9500pt"><td width="137" valign="center" style="width:102.9000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">2</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="149" valign="center" style="width:112.3500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td><td width="167" valign="center" style="width:125.3000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td><td width="191" valign="center" style="width:143.4500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td></tr><tr style="height:13.9500pt"><td width="137" valign="center" style="width:102.9000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">3</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="149" valign="center" style="width:112.3500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td><td width="167" valign="center" style="width:125.3000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td><td width="191" valign="center" style="width:143.4500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td></tr><tr style="height:28.5000pt"><td width="137" valign="center" style="width:102.9000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">Control 1</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
<p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">Control 2</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="149" valign="center" style="width:112.3500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td><td width="167" valign="center" style="width:125.3000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td><td width="191" valign="center" style="width:143.4500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td></tr>
 
</table><br>
 
<p><span style="font-family:'Times New Roman';font-size:12.0000pt">Note.1. Then concentration of </span><i><span style="font-family:'Times New Roman';font-size:12pt">Chlamydomonas reinhardtii </span></i><span style="font-family:'Times New Roman';font-size:12.0000pt">is xxx</span><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span><span style="font-family:'Times New Roman';font-size:12.0000pt">(</span><span style="font-family:'Times New Roman';font-size:12.0000pt">OD</span><sub><span style="font-family:'Times New Roman';font-size:12.0000pt;vertical-align:sub">600</span></sub><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span><span style="font-family:'Times New Roman';font-size:12.0000pt">of 1.0 = 8 x 10</span><sup><span style="font-family:'Times New Roman';font-size:12.0000pt;vertical-align:super">8</span></sup><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span><span style="font-family:'Times New Roman';font-size:12.0000pt">cells/ml, OD</span><sub><span style="font-family:'Times New Roman';font-size:12.0000pt;vertical-align:sub">600 </span></sub><span style="font-family:'Times New Roman';font-size:12.0000pt">=xxxx)</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
<p><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2. The Control 1 group is </span><i><span style="font-family:'Times New Roman';font-size:12pt">Chlamydomonas reinhardtii</span></i><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;(no transformation,</span><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;OD=?</span><span style="font-family:'Times New Roman';font-size:12.0000pt">)</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
<p><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3. The Control 2 group is TAP medium (Concentration =?)</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
<p><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
<p align="center" style="text-indent:36.0000pt;page-break-after:avoid;text-align:center"><span style="font-family:'Times New Roman';font-size:12pt">Table 2.5 Toxicity of </span><i><span style="font-family:'Times New Roman';font-size:12pt">Chlamydomonas reinhardtii </span></i><span style="font-family:'Times New Roman';font-size:12pt">(</span><i><span style="font-family:'Times New Roman';font-size:12pt">Cry11Aa+2A+Cyt1</span></i><span style="font-family:'Times New Roman';font-size:12pt">)</span><i><span style="font-family:'Times New Roman';font-size:12pt">&nbsp;</span></i><span style="font-family:'Times New Roman';font-size:12pt">to</span><i><span style="font-family:'Times New Roman';font-size:12pt">&nbsp;A. albopictus</span></i><i><span style="font-family:'Times New Roman';font-size:12pt"></span></i></p>
 
 
<table style="border-collapse:collapse;width:491.0000pt;margin-left:6.7500pt;margin-right:6.7500pt">
 
<tr style="height:21.0000pt"><td width="139" valign="center" style="width:104.3500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:1.0000pt solid windowtext;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">Repeating group</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="152" valign="center" style="width:114.0500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:1.0000pt solid windowtext;border-bottom:1.0000pt solid windowtext"><p style="margin-bottom:0.0000pt"><span style="font-family:'Times New Roman';font-size:12.0000pt">Number of insects</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="169" valign="center" style="width:127.1000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:1.0000pt solid windowtext;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">Number of mortality</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="194" valign="center" style="width:145.5000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:1.0000pt solid windowtext;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">Corrected mortality (%)</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td></tr><tr style="height:21.0000pt"><td width="139" valign="center" style="width:104.3500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:none"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">1</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="152" valign="center" style="width:114.0500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:none"><p style="margin-bottom:0.0000pt"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td><td width="169" valign="center" style="width:127.1000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:none"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td><td width="194" valign="center" style="width:145.5000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:none"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td></tr><tr style="height:13.6500pt"><td width="139" valign="center" style="width:104.3500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">2</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="152" valign="center" style="width:114.0500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td><td width="169" valign="center" style="width:127.1000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td><td width="194" valign="center" style="width:145.5000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td></tr><tr style="height:13.6500pt"><td width="139" valign="center" style="width:104.3500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">3</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="152" valign="center" style="width:114.0500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td><td width="169" valign="center" style="width:127.1000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td><td width="194" valign="center" style="width:145.5000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td></tr><tr style="height:27.9000pt"><td width="139" valign="center" style="width:104.3500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">Control 1</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
<p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">Control 2</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="152" valign="center" style="width:114.0500pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td><td width="169" valign="center" style="width:127.1000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td><td width="194" valign="center" style="width:145.5000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td></tr>
 
</table><br>
 
<p><span style="font-family:'Times New Roman';font-size:12.0000pt">Note.1. Then concentration of </span><i><span style="font-family:'Times New Roman';font-size:12pt">Chlamydomonas reinhardtii </span></i><span style="font-family:'Times New Roman';font-size:12.0000pt">is xxx</span><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span><span style="font-family:'Times New Roman';font-size:12.0000pt">(</span><span style="font-family:'Times New Roman';font-size:12.0000pt">OD</span><sub><span style="font-family:'Times New Roman';font-size:12.0000pt;vertical-align:sub">600</span></sub><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span><span style="font-family:'Times New Roman';font-size:12.0000pt">of 1.0 = 8 x 10</span><sup><span style="font-family:'Times New Roman';font-size:12.0000pt;vertical-align:super">8</span></sup><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span><span style="font-family:'Times New Roman';font-size:12.0000pt">cells/ml, OD</span><sub><span style="font-family:'Times New Roman';font-size:12.0000pt;vertical-align:sub">600 </span></sub><span style="font-family:'Times New Roman';font-size:12.0000pt">=xxxx)</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
<p><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2. The Control 1 group is </span><i><span style="font-family:'Times New Roman';font-size:12pt">Chlamydomonas reinhardtii</span></i><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;(no transformation,</span><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;OD=?</span><span style="font-family:'Times New Roman';font-size:12.0000pt">)</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
<p><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3. The Control 2 group is TAP medium (Concentration =?)</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
<p><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
<p align="center" style="text-indent:36.0000pt;page-break-after:avoid;text-align:center"><span style="font-family:'Times New Roman';font-size:12pt">Table 2.5 Toxicity of </span><i><span style="font-family:'Times New Roman';font-size:12pt">Chlamydomonas reinhardtii </span></i><span style="font-family:'Times New Roman';font-size:12pt">(</span><i><span style="font-family:'Times New Roman';font-size:12pt">Cry11Aa+2A+Cyt2</span></i><span style="font-family:'Times New Roman';font-size:12pt">)</span><i><span style="font-family:'Times New Roman';font-size:12pt">&nbsp;</span></i><span style="font-family:'Times New Roman';font-size:12pt">to</span><i><span style="font-family:'Times New Roman';font-size:12pt">&nbsp;A. albopictus</span></i><i><span style="font-family:'Times New Roman';font-size:12pt"></span></i></p>
 
 
<table style="border-collapse:collapse;width:492.0000pt;margin-left:6.7500pt;margin-right:6.7500pt">
 
<tr style="height:20.2000pt"><td width="139" valign="center" style="width:104.6000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:1.0000pt solid windowtext;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">Repeating group</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="152" valign="center" style="width:114.2000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:1.0000pt solid windowtext;border-bottom:1.0000pt solid windowtext"><p style="margin-bottom:0.0000pt"><span style="font-family:'Times New Roman';font-size:12.0000pt">Number of insects</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="169" valign="center" style="width:127.4000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:1.0000pt solid windowtext;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">Number of mortality</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="194" valign="center" style="width:145.8000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:1.0000pt solid windowtext;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">Corrected mortality (%)</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td></tr><tr style="height:20.2000pt"><td width="139" valign="center" style="width:104.6000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:none"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">1</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="152" valign="center" style="width:114.2000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:none"><p style="margin-bottom:0.0000pt"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td><td width="169" valign="center" style="width:127.4000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:none"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td><td width="194" valign="center" style="width:145.8000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-top:none"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td></tr><tr style="height:13.1000pt"><td width="139" valign="center" style="width:104.6000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">2</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="152" valign="center" style="width:114.2000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td><td width="169" valign="center" style="width:127.4000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td><td width="194" valign="center" style="width:145.8000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td></tr><tr style="height:13.7500pt"><td width="139" valign="center" style="width:104.6000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">3</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="152" valign="center" style="width:114.2000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td><td width="169" valign="center" style="width:127.4000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td><td width="194" valign="center" style="width:145.8000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td></tr><tr style="height:26.7000pt"><td width="139" valign="center" style="width:104.6000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">Control 1</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
<p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">Control 2</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
</td><td width="152" valign="center" style="width:114.2000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td><td width="169" valign="center" style="width:127.4000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td><td width="194" valign="center" style="width:145.8000pt;padding:0.0000pt 5.4000pt 0.0000pt 5.4000pt;border-bottom:1.0000pt solid windowtext"><p align="center" style="margin-bottom:0.0000pt;text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
</td></tr>
 
</table><br>
 
<p><span style="font-family:'Times New Roman';font-size:12.0000pt">Note.1. Then concentration of </span><i><span style="font-family:'Times New Roman';font-size:12pt">Chlamydomonas reinhardtii </span></i><span style="font-family:'Times New Roman';font-size:12.0000pt">is xxx</span><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span><span style="font-family:'Times New Roman';font-size:12.0000pt">(</span><span style="font-family:'Times New Roman';font-size:12.0000pt">OD</span><sub><span style="font-family:'Times New Roman';font-size:12.0000pt;vertical-align:sub">600</span></sub><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span><span style="font-family:'Times New Roman';font-size:12.0000pt">of 1.0 = 8 x 10</span><sup><span style="font-family:'Times New Roman';font-size:12.0000pt;vertical-align:super">8</span></sup><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span><span style="font-family:'Times New Roman';font-size:12.0000pt">cells/ml, OD</span><sub><span style="font-family:'Times New Roman';font-size:12.0000pt;vertical-align:sub">600 </span></sub><span style="font-family:'Times New Roman';font-size:12.0000pt">=xxxx)</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
<p><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2. The Control 1 group is </span><i><span style="font-family:'Times New Roman';font-size:12pt">Chlamydomonas reinhardtii</span></i><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;(no transformation,</span><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;OD=?</span><span style="font-family:'Times New Roman';font-size:12.0000pt">)</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
<p><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3. The Control 2 group is TAP medium (Concentration =?)</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
<p><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
<p><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
<p><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
<p><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
<p><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
<p><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
<p><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
<p><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
<p align="center" style="text-align:center"><span style="font-family:'Times New Roman';font-size:12.0000pt">Reference</span><span style="font-family:'Times New Roman';font-size:12.0000pt"></span></p>
 
<p style="margin-left:36.0000pt;text-indent:-36.0000pt"><span style="font-family:'Times New Roman';font-size:12pt">1.Zhang, L., Huang, E., Lin, J., Gelbič, I., Zhang, Q., Guan, Y., … Guan, X. (2010). A novel mosquitocidal strain LLP29 isolated from the phylloplane of. Microbiological Research, 165(2), 133–141. doi: 10.1016/j.micres.2009.03.002</span><span style="font-family:'Times New Roman';font-size:16.0000pt"></span></p>
 
<p><span style="font-family:'Times New Roman';font-size:16.0000pt">&nbsp;</span></p>
 
<p><span style="font-family:'Times New Roman';font-size:12.0000pt">&nbsp;</span></p>
 
  
 
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Latest revision as of 06:35, 8 November 2016

Contents

Team FAFU-CHINA

Parts

We constructed various basic pars, composited parts and devices to finish our project. You can find the detailed information in Basic Parts and Compose Parts Wiki pages.

T--FAFU-CHINA--parts.png

Fig 1: The Overview of 2016 FAFU-CHINA Parts