Difference between revisions of "Team:FAFU-CHINA/Basic Part"

 
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= Basic Part =
+
<div>&nbsp;<b><span style="font-size:11pt;font-family:Arial, sans-serif">Basic Part</span></b><br/>
<div>
+
  <div>
+
    <div>
+
      <div>
+
= Cry4Aa4+Extended FMDV 2A-pChlamy_3 =
+
    <table width="518" border="1" cellpadding="0" cellspacing="0">
+
         
+
            <tr>
+
              <td width="121" valign="top"><p>Gene Name </p></td>
+
              <td width="391" valign="top"><p>Cry4Aa4+Extended FMDV 2A </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Cloning Vector </p></td>
+
              <td valign="top"><p>pChlamy_3 </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Construct Resistant </p></td>
+
              <td valign="top"><p>Ampicillin in DH5α </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Gene Length </p></td>
+
              <td valign="top"><p>3673bp </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Cloning Strategy </p></td>
+
              <td valign="top"><p>XbaI and PstI </p></td>
+
            </tr>
+
         
+
        </table>
+
        <p>  </p>
+
        <table width="519" border="1" cellpadding="0" cellspacing="0">
+
         
+
            <tr>
+
              <td width="110" valign="top"><p>QC Items </p></td>
+
              <td width="125" valign="top"><p>Method </p></td>
+
              <td width="226" valign="top"><p>Specifications </p></td>
+
              <td width="48" valign="top"><p>Results </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Target Sequence </p></td>
+
              <td valign="top"><p>Sequence alignment </p></td>
+
              <td valign="top"><p>Sequencing results are consistent to the confirmed sequence. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Vector Sequence </p></td>
+
              <td valign="top"><p>Sequence alignment </p></td>
+
              <td valign="top"><p>20bp flanking sequences of the vector are correct. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Reading Frame </p></td>
+
              <td valign="top"><p>Sequence alignment </p></td>
+
              <td valign="top"><p>Frame is correct and consistent to the client's requirement. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Fragment Size </p></td>
+
              <td valign="top"><p>Restriction Digests </p></td>
+
              <td valign="top"><p>The size of inserted fragment is right and free of any contaminated bands. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Quality/Quantity </p></td>
+
              <td valign="top"><p>UV spectrophotometry </p></td>
+
              <td valign="top"><p>Miniprep: OD260/280=1.7~2.0 </p>
+
                <p>2µg/tube, 1 tube,Lyophilized </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Type of Strain Stock </p></td>
+
              <td valign="top"><p>Visual inspection </p></td>
+
              <td valign="top"><p>Bacteria stock. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Appearance </p></td>
+
              <td valign="top"><p>Visual inspection </p></td>
+
              <td valign="top"><p>Clear and free of foreign particles, and the strain is clearly visible. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
         
+
        </table>
+
        <p>  </p>
+
        <table border="1" cellpadding="0" cellspacing="0">
+
         
+
            <tr>
+
              <td valign="top"><p>https://static.igem.org/mediawiki/igem.org/c/ca/T--FAFU-CHINA--MAKERCry4Aa4ExtendedFMDV2ApChlamy3.png</p>
+
                <p>  </p></td>
+
              <td valign="top"><p><img height="294.3999938964844" src="http://b198.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/O5V87mM7vgBQcBKlBbjOuO*vaJzRp9ohwsGNYf368uI!/b/dMYAAAAAAAAA&bo=nQAnAQAAAAADAJ4!" width="156.0"></p>
+
                <p>  </p></td>
+
              <td valign="top"><p>Lane M: DNA Marker </p>
+
                <p>  </p>
+
                <p>Lane 1: Plasmid digested by XbaI and PstI </p>
+
                <p>  </p>
+
                <p>Lane 2: Plasmid DNA </p></td>
+
            </tr>
+
         
+
        </table>
+
        <p> </p>
+
        <p> </p>
+
= Cry4Aa4+Extended FMDV 2A-pChlamy_3 =
+
        <table width="519" border="1" cellpadding="0" cellspacing="0">
+
         
+
            <tr>
+
              <td width="121" valign="top"><p>Gene Name </p></td>
+
              <td width="392" valign="top"><p>Cry10Aa4+Extended FMDV 2A </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Cloning Vector </p></td>
+
              <td valign="top"><p>pChlamy_3 </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Construct Resistant </p></td>
+
              <td valign="top"><p>Ampicillin in Top10 </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Gene Length </p></td>
+
              <td valign="top"><p>2158bp </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Cloning Strategy </p></td>
+
              <td valign="top"><p>XbaI and PstI </p></td>
+
            </tr>
+
         
+
        </table>
+
        <p>  </p>
+
        <table border="1" cellpadding="0" cellspacing="0">
+
         
+
            <tr>
+
              <td valign="top"><p>QC Items </p></td>
+
              <td valign="top"><p>Method </p></td>
+
              <td valign="top"><p>Specifications </p></td>
+
              <td valign="top"><p>Results </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Target Sequence </p></td>
+
              <td valign="top"><p>Sequence alignment </p></td>
+
              <td valign="top"><p>Sequencing results are consistent to the confirmed sequence. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Vector Sequence </p></td>
+
              <td valign="top"><p>Sequence alignment </p></td>
+
              <td valign="top"><p>20bp flanking sequences of the vector are correct. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Reading Frame </p></td>
+
              <td valign="top"><p>Sequence alignment </p></td>
+
              <td valign="top"><p>Frame is correct and consistent to the client's requirement. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Fragment Size </p></td>
+
              <td valign="top"><p>Restriction Digests </p></td>
+
              <td valign="top"><p>The size of inserted fragment is right and free of any contaminated bands. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Quality/Quantity </p></td>
+
              <td valign="top"><p>UV spectrophotometry </p></td>
+
              <td valign="top"><p>Miniprep: OD260/280=1.7~2.0 </p>
+
                <p>2µg/tube, 1 tube,Lyophilized </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Type of Strain Stock </p></td>
+
              <td valign="top"><p>Visual inspection </p></td>
+
              <td valign="top"><p>Bacteria stock. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Appearance </p></td>
+
              <td valign="top"><p>Visual inspection </p></td>
+
              <td valign="top"><p>Clear and free of foreign particles, and the strain is clearly visible. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
         
+
        </table>
+
        <p>  </p>
+
        <table border="1" cellpadding="0" cellspacing="0">
+
         
+
            <tr>
+
              <td valign="top"><p><img height="254.39999389648438" src="http://b262.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/bQ8OOekpGDUmt.8vkQvJx0XUpn6PMh1VgaiF0dasSRg!/b/dAYBAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422"></p>
+
                <p>  </p></td>
+
              <td valign="top"><p><img height="308.0" src="http://b199.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/LjSeZIndHm9zkUE6gT36bEx3VnYrEwCbYS7Aq.zBlqw!/b/dMcAAAAAAAAA&bo=7QA1AQAAAAADAPw!" width="236.8000030517578"></p>
+
                <p>  </p></td>
+
              <td valign="top"><p>Lane M: DNA Marker </p>
+
                <p>  </p>
+
                <p>Lane 1: Plasmid digested by XbaI and PstI </p>
+
                <p>  </p>
+
                <p>Lane 2: Plasmid DNA </p></td>
+
            </tr>
+
         
+
        </table>
+
        <p> </p>
+
        <p> </p>
+
= Cry11Aa4+ Extended FMDV 2A-pChlamy_3 =
+
        <table width="519" border="1" cellpadding="0" cellspacing="0">
+
         
+
            <tr>
+
              <td width="121" valign="top"><p>Gene Name </p></td>
+
              <td width="392" valign="top"><p>Cry11Aa4+ Extended FMDV 2A </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Cloning Vector </p></td>
+
              <td valign="top"><p>pChlamy_3 </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Construct Resistant </p></td>
+
              <td valign="top"><p>Ampicillin in JM108 </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Gene Length </p></td>
+
              <td valign="top"><p>2071bp </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Cloning Strategy </p></td>
+
              <td valign="top"><p>XbaI and PstI </p></td>
+
            </tr>
+
         
+
        </table>
+
        <p>  </p>
+
        <table border="1" cellpadding="0" cellspacing="0">
+
         
+
            <tr>
+
              <td valign="top"><p>QC Items </p></td>
+
              <td valign="top"><p>Method </p></td>
+
              <td valign="top"><p>Specifications </p></td>
+
              <td valign="top"><p>Results </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Target Sequence </p></td>
+
              <td valign="top"><p>Sequence alignment </p></td>
+
              <td valign="top"><p>Sequencing results are consistent to the confirmed sequence. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Vector Sequence </p></td>
+
              <td valign="top"><p>Sequence alignment </p></td>
+
              <td valign="top"><p>20bp flanking sequences of the vector are correct. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Reading Frame </p></td>
+
              <td valign="top"><p>Sequence alignment </p></td>
+
              <td valign="top"><p>Frame is correct and consistent to the client's requirement. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Fragment Size </p></td>
+
              <td valign="top"><p>Restriction Digests </p></td>
+
              <td valign="top"><p>The size of inserted fragment is right and free of any contaminated bands. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Quality/Quantity </p></td>
+
              <td valign="top"><p>UV spectrophotometry </p></td>
+
              <td valign="top"><p>Miniprep: OD260/280=1.7~2.0 </p>
+
                <p>2µg/tube, 1 tube,Lyophilized </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Type of Strain Stock </p></td>
+
              <td valign="top"><p>Visual inspection </p></td>
+
              <td valign="top"><p>Bacteria stock. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
 
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Appearance </p></td>
+
              <td valign="top"><p>Visual inspection </p></td>
+
              <td valign="top"><p>Clear and free of foreign particles, and the strain is clearly visible. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
         
+
        </table>
+
        <p>  </p>
+
        <table width="519" border="1" cellpadding="0" cellspacing="0">
+
         
+
            <tr>
+
              <td width="175" valign="top"><p><img height="254.39999389648438" src="http://b198.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/d0.upRVhjL7OQjfkLGru*.dUocUXc4fDAJpeQEXjZe8!/b/dMYAAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422"></p>
+
                <p>  </p></td>
+
              <td width="167" valign="top"><p><img height="308.0" src="http://b198.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/0MvndoK9mmRt.kJuTifErWbDLJSPJui*8L*GLlyeGl8!/b/dMYAAAAAAAAA&bo=qAA1AQAAAAADALk!" width="167.1999969482422"></p></td>
+
              <td width="169" valign="top"><p>Lane M: DNA Marker </p>
+
                <p>  </p>
+
                <p>Lane 1: Plasmid digested by XbaI and PstI </p>
+
                <p>  </p>
+
                <p>Lane 2: Plasmid DNA </p>
+
                <p>  </p></td>
+
            </tr>
+
         
+
        </table>
+
        <p> </p>
+
        <p> </p>
+
= <p>Cyt1-pChlamy_3 =
+
        <table width="515" border="1" cellpadding="0" cellspacing="0">
+
         
+
            <tr>
+
              <td width="121" valign="top"><p>Gene Name </p></td>
+
              <td width="391" valign="top"><p>Cyt1 </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Cloning Vector </p></td>
+
              <td valign="top"><p>pChlamy_3 </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Construct Resistant </p></td>
+
              <td valign="top"><p>Ampicillin in JM108 </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Gene Length </p></td>
+
              <td valign="top"><p>762bp </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Cloning Strategy </p></td>
+
              <td valign="top"><p>XbaI and PstI </p></td>
+
            </tr>
+
         
+
        </table>
+
        <p>  </p>
+
        <table width="519" border="1" cellpadding="0" cellspacing="0">
+
         
+
            <tr>
+
              <td width="110" valign="top"><p>QC Items </p></td>
+
              <td width="125" valign="top"><p>Method </p></td>
+
              <td width="226" valign="top"><p>Specifications </p></td>
+
              <td width="48" valign="top"><p>Results </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Target Sequence </p></td>
+
              <td valign="top"><p>Sequence alignment </p></td>
+
              <td valign="top"><p>Sequencing results are consistent to the confirmed sequence. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Vector Sequence </p></td>
+
              <td valign="top"><p>Sequence alignment </p></td>
+
              <td valign="top"><p>20bp flanking sequences of the vector are correct. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Reading Frame </p></td>
+
              <td valign="top"><p>Sequence alignment </p></td>
+
              <td valign="top"><p>Frame is correct and consistent to the client's requirement. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Fragment Size </p></td>
+
              <td valign="top"><p>Restriction Digests </p></td>
+
              <td valign="top"><p>The size of inserted fragment is right and free of any contaminated bands. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Quality/Quantity </p></td>
+
              <td valign="top"><p>UV spectrophotometry </p></td>
+
              <td valign="top"><p>Miniprep: OD260/280=1.7~2.0 </p>
+
                <p>2µg/tube, 1 tube,Lyophilized </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Type of Strain Stock </p></td>
+
              <td valign="top"><p>Visual inspection </p></td>
+
              <td valign="top"><p>Bacteria stock. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Appearance </p></td>
+
              <td valign="top"><p>Visual inspection </p></td>
+
              <td valign="top"><p>Clear and free of foreign particles, and the strain is clearly visible. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
         
+
        </table>
+
        <p>  </p>
+
        <table width="519" border="1" cellpadding="0" cellspacing="0">
+
         
+
            <tr>
+
              <td width="175" valign="top"><p><img height="254.39999389648438" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/Sy.SKFU5edTQSIF.h2uwVTZjSgxUj3zmtAOCAGbAaZo!/b/dMgAAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422"></p>
+
                <p>  </p></td>
+
              <td width="175" valign="top"><p><img height="279.20001220703125" src="http://b264.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/xncHHhLWQz1aqSpaH4UR7R5bMZ4zFZ*PmV1nZxwVTQ8!/b/dAgBAAAAAAAA&bo=sAAYAQAAAAADAIw!" width="175.1999969482422"></p></td>
+
              <td width="161" valign="top"><p>  </p>
+
                <p>Lane M: DNA Marker </p>
+
                <p>  </p>
+
                <p>Lane 1: Plasmid digested by XbaI and PstI </p>
+
                <p>  </p>
+
                <p>Lane 2: Plasmid DNA </p>
+
                <p>  </p>
+
                <p>  </p></td>
+
            </tr>
+
         
+
        </table>
+
        <p> </p>
+
        <p> </p>
+
= Cyt2-pChlamy_3 =
+
        <table width="519" border="1" cellpadding="0" cellspacing="0">
+
         
+
            <tr>
+
              <td width="117" valign="top"><p>Gene Name </p></td>
+
              <td width="396" valign="top"><p>Cyt1 </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Cloning Vector </p></td>
+
              <td valign="top"><p>pChlamy_3 </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Construct Resistant </p></td>
+
              <td valign="top"><p>Ampicillin in JM108 </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Gene Length </p></td>
+
              <td valign="top"><p>801bp </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Cloning Strategy </p></td>
+
              <td valign="top"><p>XbaI and PstI </p></td>
+
            </tr>
+
         
+
        </table>
+
        <p>  </p>
+
        <table width="519" border="1" cellpadding="0" cellspacing="0">
+
         
+
            <tr>
+
              <td width="131" valign="top"><p>QC Items </p></td>
+
              <td colspan="2" valign="top"><p>Method </p></td>
+
              <td colspan="2" valign="top"><p>Specifications </p></td>
+
              <td colspan="2" valign="top"><p>Results </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Target Sequence </p></td>
+
              <td colspan="2" valign="top"><p>Sequence alignment </p></td>
+
              <td colspan="2" valign="top"><p>Sequencing results are consistent to the confirmed sequence. </p></td>
+
              <td colspan="2" valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Vector Sequence </p></td>
+
              <td colspan="2" valign="top"><p>Sequence alignment </p></td>
+
              <td colspan="2" valign="top"><p>20bp flanking sequences of the vector are correct. </p></td>
+
              <td colspan="2" valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Reading Frame </p></td>
+
              <td colspan="2" valign="top"><p>Sequence alignment </p></td>
+
              <td colspan="2" valign="top"><p>Frame is correct and consistent to the client's requirement. </p></td>
+
              <td colspan="2" valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Fragment Size </p></td>
+
              <td colspan="2" valign="top"><p>Restriction Digests </p></td>
+
              <td colspan="2" valign="top"><p>The size of inserted fragment is right and free of any contaminated bands. </p></td>
+
              <td colspan="2" valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Quality/Quantity </p></td>
+
              <td colspan="2" valign="top"><p>UV spectrophotometry </p></td>
+
              <td colspan="2" valign="top"><p>Miniprep: OD260/280=1.7~2.0 </p>
+
                <p>2µg/tube, 1 tube,Lyophilized </p></td>
+
              <td colspan="2" valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Type of Strain Stock </p></td>
+
              <td colspan="2" valign="top"><p>Visual inspection </p></td>
+
              <td colspan="2" valign="top"><p>Bacteria stock. </p></td>
+
              <td colspan="2" valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td height="74" valign="top"><p>Appearance </p></td>
+
              <td colspan="2" valign="top"><p>Visual inspection </p></td>
+
              <td colspan="2" valign="top"><p>Clear and free of foreign particles, and the strain is clearly visible. </p></td>
+
              <td colspan="2" valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td colspan="2" valign="top"><p><img height="254.39999389648438" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/d0mowbkc0M494OfDGRHIGtmKGQNkXPPWleN7nPIwSf0!/b/dMgAAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422"></p>
+
                <p>  </p></td>
+
              <td colspan="2" valign="top"><p><img height="280.0" src="http://b262.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/irbPRy7*zARuesyKRBXZFk6ZWMGm44ZwwyjWYNaqYZA!/b/dAYBAAAAAAAA&bo=4QAZAQAAAAADANw!" width="224.0"></p></td>
+
              <td colspan="2" valign="top"><p>  </p>
+
                <p>Lane M: DNA Marker </p>
+
                <p>  </p>
+
                <p>Lane 1: Plasmid digested by XbaI and PstI </p>
+
                <p>  </p>
+
                <p>Lane 2: Plasmid DNA </p>
+
                <p>  </p>
+
                <p>  </p></td>
+
              <td width="46"><p> </p></td>
+
            </tr>
+
            <tr height="0">
+
              <td></td>
+
              <td width="42"></td>
+
              <td width="119"></td>
+
              <td width="105"></td>
+
              <td width="60"></td>
+
              <td width="1"></td>
+
              <td></td>
+
            </tr>
+
         
+
        </table>
+
        <p> </p>
+
        <p> </p>
+
        <p> </p>
+
= EGFP-pChlamy_3 =
+
        <table width="519" border="1" cellpadding="0" cellspacing="0">
+
         
+
            <tr>
+
              <td width="121" valign="top"><p>Gene Name </p></td>
+
              <td width="392" valign="top"><p>06-EGFP(TAA) </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Cloning Vector </p></td>
+
              <td valign="top"><p>pChlamy_3 </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Construct Resistant </p></td>
+
              <td valign="top"><p>Ampicillin in Top10 </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Gene Length </p></td>
+
              <td valign="top"><p>732bp </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Cloning Strategy </p></td>
+
              <td valign="top"><p>XbaI and PstI </p></td>
+
            </tr>
+
         
+
        </table>
+
        <p>  </p>
+
        <table width="519" border="1" cellpadding="0" cellspacing="0">
+
         
+
            <tr>
+
              <td width="110" valign="top"><p>QC Items </p></td>
+
              <td width="125" valign="top"><p>Method </p></td>
+
              <td width="226" valign="top"><p>Specifications </p></td>
+
              <td width="48" valign="top"><p>Results </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Target Sequence </p></td>
+
              <td valign="top"><p>Sequence alignment </p></td>
+
              <td valign="top"><p>Sequencing results are consistent to the confirmed sequence. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Vector Sequence </p></td>
+
              <td valign="top"><p>Sequence alignment </p></td>
+
              <td valign="top"><p>20bp flanking sequences of the vector are correct. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Reading Frame </p></td>
+
              <td valign="top"><p>Sequence alignment </p></td>
+
              <td valign="top"><p>Frame is correct and consistent to the client's requirement. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Fragment Size </p></td>
+
              <td valign="top"><p>Restriction Digests </p></td>
+
              <td valign="top"><p>The size of inserted fragment is right and free of any contaminated bands. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Quality/Quantity </p></td>
+
              <td valign="top"><p>UV spectrophotometry </p></td>
+
              <td valign="top"><p>Miniprep: OD260/280=1.7~2.0 </p>
+
                <p>2µg/tube, 1 tube,Lyophilized </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Type of Strain Stock </p></td>
+
              <td valign="top"><p>Visual inspection </p></td>
+
              <td valign="top"><p>Bacteria stock. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Appearance </p></td>
+
              <td valign="top"><p>Visual inspection </p></td>
+
              <td valign="top"><p>Clear and free of foreign particles, and the strain is clearly visible. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
         
+
        </table>
+
        <p>  </p>
+
        <table width="519" border="1" cellpadding="0" cellspacing="0">
+
         
+
            <tr>
+
              <td width="175" valign="top"><p><img height="254.39999389648438" src="http://b260.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/kV2mqmj1q8v1ump215nFHpnjMUzwxqSu0DronaWKuIs!/b/dAQBAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422"></p></td>
+
              <td width="239" valign="top"><p><img height="296.0" src="http://b199.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/aRGJqrgiOwOJDeH5hDKr.6c7zKU8VMfcgFHbm35PZ6I!/b/dMcAAAAAAAAA&bo=8AApAQAAAAADAP0!" width="239.1999969482422"></p></td>
+
              <td width="97" valign="top"><p>  </p>
+
                <p>Lane M: DNA Marker </p>
+
                <p>  </p>
+
                <p>Lane 1: Plasmid digested by XbaI and PstI </p>
+
                <p>  </p>
+
                <p>Lane 2: Plasmid DNA </p>
+
                <p>  </p>
+
                <p>  </p></td>
+
            </tr>
+
         
+
        </table>
+
        <p> </p>
+
= Cry4Aa4+Extended2A+Cyt2-pChlamy3 =
+
        <table width="519" border="1" cellpadding="0" cellspacing="0">
+
         
+
            <tr>
+
              <td width="120" valign="top"><p>Gene Name </p></td>
+
              <td width="393" valign="top"><p>Cry4Aa4+Extended2A+Cyt2 </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Cloning Vector </p></td>
+
              <td valign="top"><p>pChlamy_3 </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Construct Resistant </p></td>
+
              <td valign="top"><p>Ampicillin in Top10 </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Gene Length </p></td>
+
              <td valign="top"><p>4461bp </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Cloning Strategy </p></td>
+
              <td valign="top"><p>XbaI and PstI </p></td>
+
            </tr>
+
         
+
        </table>
+
        <p>  </p>
+
        <table width="519" border="1" cellpadding="0" cellspacing="0">
+
         
+
            <tr>
+
              <td width="110" valign="top"><p>QC Items </p></td>
+
              <td width="125" valign="top"><p>Method </p></td>
+
              <td width="226" valign="top"><p>Specifications </p></td>
+
              <td width="48" valign="top"><p>Results </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Target Sequence </p></td>
+
              <td valign="top"><p>Sequence alignment </p></td>
+
              <td valign="top"><p>Sequencing results are consistent to the confirmed sequence. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Vector Sequence </p></td>
+
              <td valign="top"><p>Sequence alignment </p></td>
+
              <td valign="top"><p>20bp flanking sequences of the vector are correct. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Reading Frame </p></td>
+
              <td valign="top"><p>Sequence alignment </p></td>
+
              <td valign="top"><p>Frame is correct and consistent to the client's requirement. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Fragment Size </p></td>
+
              <td valign="top"><p>Restriction Digests </p></td>
+
              <td valign="top"><p>The size of inserted fragment is right and free of any contaminated bands. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Quality/Quantity </p></td>
+
              <td valign="top"><p>UV spectrophotometry </p></td>
+
              <td valign="top"><p>Miniprep: OD260/280=1.7~2.0 </p>
+
                <p>2µg/tube, 1 tube,Lyophilized </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Type of Strain Stock </p></td>
+
              <td valign="top"><p>Visual inspection </p></td>
+
              <td valign="top"><p>Bacteria stock. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Appearance </p></td>
+
              <td valign="top"><p>Visual inspection </p></td>
+
              <td valign="top"><p>Clear and free of foreign particles, and the strain is clearly visible. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
         
+
        </table>
+
        <p>  </p>
+
        <table width="519" border="1" cellpadding="0" cellspacing="0">
+
         
+
            <tr>
+
              <td width="168" valign="top"><p><img height="254.39999389648438" src="http://b199.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/uN*b2mkL9gaipMv8XpN.4wpt8raBNnOQuGwbKC.w*vE!/b/dMcAAAAAAAAA&bo=qQD*AAAAAAADAHM!" width="168.8000030517578"></p>
+
                <p>  </p></td>
+
              <td width="151" valign="top"><p><img height="236.0" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/JI.J1CeIa*MhQu9aafo8T8eZeOr7zqxlK5q6W3NjbEA!/b/dMgAAAAAAAAA&bo=mADtAAAAAAADAFA!" width="151.1999969482422"></p>
+
                <p>  </p></td>
+
              <td width="192" valign="top"><p>  </p>
+
                <p>Lane M: DNA Marker </p>
+
                <p>  </p>
+
                <p>Lane 1: Plasmid digested by XbaI and BamHI </p>
+
                <p>  </p>
+
                <p>Lane 2: Plasmid DNA </p>
+
                <p>  </p>
+
                <p>  </p>
+
                <p>  </p></td>
+
            </tr>
+
         
+
        </table>
+
        <p>  </p>
+
        <p> </p>
+
        <p> </p>
+
= Cry4Aa4+Extended2A+Cyt1-pChlamy3 =
+
<table width="519" border="1" cellpadding="0" cellspacing="0">
+
         
+
            <tr>
+
              <td width="115" valign="top"><p>Gene Name </p></td>
+
              <td width="398" valign="top"><p>Cry4Aa4+Extended2A+Cyt1-pChlamy3 </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Cloning Vector </p></td>
+
              <td valign="top"><p>pChlamy_3 </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Construct Resistant </p></td>
+
              <td valign="top"><p>Ampicillin in JM108 </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Gene Length </p></td>
+
              <td valign="top"><p>4422bp </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Cloning Strategy </p></td>
+
              <td valign="top"><p>XbaI and PstI </p></td>
+
            </tr>
+
         
+
        </table>
+
        <p>  </p>
+
<table width="519" border="1" cellpadding="0" cellspacing="0">
+
         
+
            <tr>
+
              <td width="132" valign="top"><p>QC Items </p></td>
+
              <td colspan="2" valign="top"><p>Method </p></td>
+
              <td colspan="3" valign="top"><p>Specifications </p></td>
+
              <td width="50" valign="top"><p>Results </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Target Sequence </p></td>
+
              <td colspan="2" valign="top"><p>Sequence alignment </p></td>
+
              <td colspan="3" valign="top"><p>Sequencing results are consistent to the confirmed sequence. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Vector Sequence </p></td>
+
              <td colspan="2" valign="top"><p>Sequence alignment </p></td>
+
              <td colspan="3" valign="top"><p>20bp flanking sequences of the vector are correct. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Reading Frame </p></td>
+
              <td colspan="2" valign="top"><p>Sequence alignment </p></td>
+
              <td colspan="3" valign="top"><p>Frame is correct and consistent to the client's requirement. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Fragment Size </p></td>
+
              <td colspan="2" valign="top"><p>Restriction Digests </p></td>
+
              <td colspan="3" valign="top"><p>The size of inserted fragment is right and free of any contaminated bands. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Quality/Quantity </p></td>
+
              <td colspan="2" valign="top"><p>UV spectrophotometry </p></td>
+
              <td colspan="3" valign="top"><p>Miniprep: OD260/280=1.7~2.0 </p>
+
                <p>2µg/tube, 1 tube,Lyophilized </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Type of Strain Stock </p></td>
+
              <td colspan="2" valign="top"><p>Visual inspection </p></td>
+
              <td colspan="3" valign="top"><p>Bacteria stock. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Appearance </p></td>
+
              <td colspan="2" valign="top"><p>Visual inspection </p></td>
+
              <td colspan="3" valign="top"><p>Clear and free of foreign particles, and the strain is clearly visible. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td colspan="2" valign="top"><p>  </p>
+
                <p><img height="254.39999389648438" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/pDvRNLD368siSdiKOdhck0A80upc24m2Aqj5qn.yyfY!/b/dMgAAAAAAAAA&bo=qQD*AAAAAAADAHM!" width="168.8000030517578"></p></td>
+
              <td colspan="2" valign="top"><p><img height="280.0" src="http://b199.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/LqdjCnlIs0z8YJ*6kbyj5ACC8gGPT6IlK2OdWcxc4T0!/b/dMcAAAAAAAAA&bo=rgAZAQAAAAADAJM!" width="173.60000610351562"></p></td>
+
              <td width="97" valign="top"><p>  </p>
+
                <p>Lane M: DNA Marker </p>
+
                <p>  </p>
+
                <p>Lane 1: Plasmid digested by BamHI and KpnI </p>
+
                <p>  </p>
+
                <p>Lane 2: Plasmid DNA </p>
+
                <p>  </p>
+
                <p>  </p></td>
+
              <td colspan="2"><p> </p></td>
+
            </tr>
+
            <tr height="0">
+
              <td></td>
+
              <td width="34"></td>
+
              <td width="124"></td>
+
              <td width="47"></td>
+
              <td></td>
+
              <td width="19"></td>
+
              <td></td>
+
            </tr>
+
         
+
        </table>
+
= Cry10Aa4+Extended2A+Cyt2-pChlamy3 =
+
        <p>  </p>
+
        <table width="519" border="1" cellpadding="0" cellspacing="0">
+
         
+
            <tr>
+
              <td width="117" valign="top"><p>Gene Name </p></td>
+
              <td width="396" valign="top"><p>Cry10Aa4+Extended2A+Cyt2-pChlamy3 </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Cloning Vector </p></td>
+
              <td valign="top"><p>pChlamy_3 </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Construct Resistant </p></td>
+
              <td valign="top"><p>Ampicillin in DH5α </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Gene Length </p></td>
+
              <td valign="top"><p>2946bp </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Cloning Strategy </p></td>
+
              <td valign="top"><p>XbaI and PstI </p></td>
+
            </tr>
+
         
+
        </table>
+
        <p>  </p>
+
        <table width="519" border="1" cellpadding="0" cellspacing="0">
+
         
+
            <tr>
+
              <td width="110" valign="top"><p>QC Items </p></td>
+
              <td width="125" valign="top"><p>Method </p></td>
+
              <td width="226" valign="top"><p>Specifications </p></td>
+
              <td width="48" valign="top"><p>Results </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Target Sequence </p></td>
+
              <td valign="top"><p>Sequence alignment </p></td>
+
              <td valign="top"><p>Sequencing results are consistent to the confirmed sequence. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Vector Sequence </p></td>
+
              <td valign="top"><p>Sequence alignment </p></td>
+
              <td valign="top"><p>20bp flanking sequences of the vector are correct. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Reading Frame </p></td>
+
              <td valign="top"><p>Sequence alignment </p></td>
+
              <td valign="top"><p>Frame is correct and consistent to the client's requirement. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Fragment Size </p></td>
+
              <td valign="top"><p>Restriction Digests </p></td>
+
              <td valign="top"><p>The size of inserted fragment is right and free of any contaminated bands. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Quality/Quantity </p></td>
+
              <td valign="top"><p>UV spectrophotometry </p></td>
+
              <td valign="top"><p>Miniprep: OD260/280=1.7~2.0 </p>
+
                <p>2µg/tube, 1 tube,Lyophilized </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Type of Strain Stock </p></td>
+
              <td valign="top"><p>Visual inspection </p></td>
+
              <td valign="top"><p>Bacteria stock. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Appearance </p></td>
+
              <td valign="top"><p>Visual inspection </p></td>
+
              <td valign="top"><p>Clear and free of foreign particles, and the strain is clearly visible. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
         
+
        </table>
+
        <p>  </p>
+
        <table width="519" border="1" cellpadding="0" cellspacing="0">
+
         
+
            <tr>
+
              <td width="175" valign="top"><p><img height="254.39999389648438" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/H1*GSbsbhQbTR1eeNDiS8fA5AfLGIser.g5DKjHyGtU!/b/dMgAAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422"></p></td>
+
              <td width="140" valign="top"><p><img height="264.0" src="http://b198.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/JfiiusuOJGnfyciEpeNFbNeC5oqcVaFFkIBoPRxw6x0!/b/dMYAAAAAAAAA&bo=jQAJAQAAAAADAKA!" width="140.0"></p></td>
+
              <td width="196" valign="top"><p>  </p>
+
                <p>Lane M: DNA Marker </p>
+
                <p>  </p>
+
                <p>Lane 1: Plasmid digested by XbaI and PstI </p>
+
                <p>  </p>
+
                <p>Lane 2: Plasmid DNA </p>
+
                <p>  </p>
+
                <p>  </p>
+
                <p>  </p></td>
+
            </tr>
+
         
+
        </table>
+
        <p> </p>
+
        <p> </p>
+
= Cry10Aa4+Extended2A+Cyt2-pChlamy3 =
+
        <table width="519" border="1" cellpadding="0" cellspacing="0">
+
         
+
            <tr>
+
              <td width="117" valign="top"><p>Gene Name </p></td>
+
              <td width="396" valign="top"><p>Cry10Aa4+Extended2A+Cyt2-pChlamy3 </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Cloning Vector </p></td>
+
              <td valign="top"><p>pChlamy_3 </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Construct Resistant </p></td>
+
              <td valign="top"><p>Ampicillin in DH5α </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Gene Length </p></td>
+
              <td valign="top"><p>2946bp </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Cloning Strategy </p></td>
+
              <td valign="top"><p>XbaI and PstI </p></td>
+
            </tr>
+
         
+
        </table>
+
        <p>  </p>
+
        <table width="519" border="1" cellpadding="0" cellspacing="0">
+
         
+
            <tr>
+
              <td width="110" valign="top"><p>QC Items </p></td>
+
              <td width="125" valign="top"><p>Method </p></td>
+
              <td width="226" valign="top"><p>Specifications </p></td>
+
              <td width="48" valign="top"><p>Results </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Target Sequence </p></td>
+
              <td valign="top"><p>Sequence alignment </p></td>
+
              <td valign="top"><p>Sequencing results are consistent to the confirmed sequence. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Vector Sequence </p></td>
+
              <td valign="top"><p>Sequence alignment </p></td>
+
              <td valign="top"><p>20bp flanking sequences of the vector are correct. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Reading Frame </p></td>
+
              <td valign="top"><p>Sequence alignment </p></td>
+
              <td valign="top"><p>Frame is correct and consistent to the client's requirement. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Fragment Size </p></td>
+
              <td valign="top"><p>Restriction Digests </p></td>
+
              <td valign="top"><p>The size of inserted fragment is right and free of any contaminated bands. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Quality/Quantity </p></td>
+
              <td valign="top"><p>UV spectrophotometry </p></td>
+
              <td valign="top"><p>Miniprep: OD260/280=1.7~2.0 </p>
+
                <p>2µg/tube, 1 tube,Lyophilized </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Type of Strain Stock </p></td>
+
              <td valign="top"><p>Visual inspection </p></td>
+
              <td valign="top"><p>Bacteria stock. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Appearance </p></td>
+
              <td valign="top"><p>Visual inspection </p></td>
+
              <td valign="top"><p>Clear and free of foreign particles, and the strain is clearly visible. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
         
+
        </table>
+
        <p>  </p>
+
        <table width="519" border="1" cellpadding="0" cellspacing="0">
+
         
+
            <tr>
+
              <td width="175" valign="top"><p><img height="254.39999389648438" src="http://b199.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/9p5OCN4X0ji10gEBq68TdCUN93aBGaDnJIBTc5E0f3M!/b/dMcAAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422"></p>
+
                <p>  </p></td>
+
              <td width="144" valign="top"><p><img height="273.6000061035156" src="http://b259.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/1zft.p5lOoX2yVoxccDyfQWcQjbGRb2SD*Zq97vSedo!/b/dAMBAAAAAAAA&bo=kQASAQAAAAADAKc!" width="144.8000030517578"></p></td>
+
              <td width="192" valign="top"><p>  </p>
+
                <p>Lane M: DNA Marker </p>
+
                <p>  </p>
+
                <p>Lane 1: Plasmid digested by XbaI and PstI </p>
+
                <p>  </p>
+
                <p>Lane 2: Plasmid DNA </p>
+
                <p>  </p>
+
                <p>  </p>
+
                <p>  </p></td>
+
            </tr>
+
         
+
        </table>
+
= Cry10Aa4+Extended2A+Cyt1-pChlamy3 =
+
<table border="1" cellpadding="0" cellspacing="0">
+
         
+
            <tr>
+
              <td width="121" valign="top"><p>Gene Name </p></td>
+
              <td width="410" valign="top"><p>Cry10Aa4+Extended2A+Cyt1-pChlamy3 </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Cloning Vector </p></td>
+
              <td valign="top"><p>pChlamy_3 </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Construct Resistant </p></td>
+
              <td valign="top"><p>Ampicillin in DH5α </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Gene Length </p></td>
+
              <td valign="top"><p>2907bp </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Cloning Strategy </p></td>
+
              <td valign="top"><p>XbaI and PstI </p></td>
+
            </tr>
+
         
+
        </table>
+
        <p>  </p>
+
        <table width="519" border="1" cellpadding="0" cellspacing="0">
+
         
+
            <tr>
+
              <td width="131" valign="top"><p>QC Items </p></td>
+
              <td colspan="2" valign="top"><p>Method </p></td>
+
              <td colspan="3" valign="top"><p>Specifications </p></td>
+
              <td width="48" valign="top"><p>Results </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Target Sequence </p></td>
+
              <td colspan="2" valign="top"><p>Sequence alignment </p></td>
+
              <td colspan="3" valign="top"><p>Sequencing results are consistent to the confirmed sequence. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Vector Sequence </p></td>
+
              <td colspan="2" valign="top"><p>Sequence alignment </p></td>
+
              <td colspan="3" valign="top"><p>20bp flanking sequences of the vector are correct. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Reading Frame </p></td>
+
              <td colspan="2" valign="top"><p>Sequence alignment </p></td>
+
              <td colspan="3" valign="top"><p>Frame is correct and consistent to the client's requirement. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Fragment Size </p></td>
+
              <td colspan="2" valign="top"><p>Restriction Digests </p></td>
+
              <td colspan="3" valign="top"><p>The size of inserted fragment is right and free of any contaminated bands. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Quality/Quantity </p></td>
+
              <td colspan="2" valign="top"><p>UV spectrophotometry </p></td>
+
              <td colspan="3" valign="top"><p>Miniprep: OD260/280=1.7~2.0 </p>
+
                <p>2µg/tube, 1 tube,Lyophilized </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Type of Strain Stock </p></td>
+
              <td colspan="2" valign="top"><p>Visual inspection </p></td>
+
              <td colspan="3" valign="top"><p>Bacteria stock. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Appearance </p></td>
+
              <td colspan="2" valign="top"><p>Visual inspection </p></td>
+
              <td colspan="3" valign="top"><p>Clear and free of foreign particles, and the strain is clearly visible. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td colspan="2" valign="top"><p><img height="254.39999389648438" src="http://b262.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/qchMjMQfSkqMMyaq5LhKw3ZuaGXoJRnrEvO0Slo0S1A!/b/dAYBAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422"></p>
+
                <p>  </p></td>
+
              <td colspan="2" valign="top"><p><img height="288.79998779296875" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/Rme26s7JjvB5bA9BlIrNu00W2reMmKwHkx9yaus6ikU!/b/dMgAAAAAAAAA&bo=zQAhAQAAAAADAMg!" width="204.8000030517578"></p></td>
+
              <td width="78" valign="top"><p>  </p>
+
                <p>Lane M: DNA Marker </p>
+
                <p>  </p>
+
                <p>Lane 1: Plasmid digested by XbaI and PstI </p>
+
                <p>  </p>
+
                <p>Lane 2: Plasmid DNA </p>
+
                <p>  </p>
+
                <p>  </p></td>
+
              <td colspan="2"><p> </p></td>
+
            </tr>
+
            <tr height="0">
+
              <td></td>
+
              <td width="42"></td>
+
              <td width="127"></td>
+
              <td width="75"></td>
+
              <td></td>
+
              <td width="2"></td>
+
              <td></td>
+
            </tr>
+
         
+
        </table>
+
= Cry11Aa4+Extended2A+Cyt2-pChlamy3 =
+
        <table width="519" border="1" cellpadding="0" cellspacing="0">
+
         
+
            <tr>
+
              <td width="117" valign="top"><p>Gene Name </p></td>
+
              <td width="396" valign="top"><p>Cry11Aa4+Extended2A+Cyt2-pChlamy3 </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Cloning Vector </p></td>
+
              <td valign="top"><p>pChlamy_3 </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Construct Resistant </p></td>
+
              <td valign="top"><p>Ampicillin in Top10 </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Gene Length </p></td>
+
              <td valign="top"><p>2859bp </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Cloning Strategy </p></td>
+
              <td valign="top"><p>XbaI and PstI </p></td>
+
            </tr>
+
         
+
        </table>
+
        <p>  </p>
+
        <table width="519" border="1" cellpadding="0" cellspacing="0">
+
         
+
            <tr>
+
              <td width="110" valign="top"><p>QC Items </p></td>
+
              <td width="125" valign="top"><p>Method </p></td>
+
              <td width="226" valign="top"><p>Specifications </p></td>
+
              <td width="48" valign="top"><p>Results </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Target Sequence </p></td>
+
              <td valign="top"><p>Sequence alignment </p></td>
+
              <td valign="top"><p>Sequencing results are consistent to the confirmed sequence. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Vector Sequence </p></td>
+
              <td valign="top"><p>Sequence alignment </p></td>
+
              <td valign="top"><p>20bp flanking sequences of the vector are correct. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Reading Frame </p></td>
+
              <td valign="top"><p>Sequence alignment </p></td>
+
              <td valign="top"><p>Frame is correct and consistent to the client's requirement. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Fragment Size </p></td>
+
              <td valign="top"><p>Restriction Digests </p></td>
+
              <td valign="top"><p>The size of inserted fragment is right and free of any contaminated bands. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Quality/Quantity </p></td>
+
              <td valign="top"><p>UV spectrophotometry </p></td>
+
              <td valign="top"><p>Miniprep: OD260/280=1.7~2.0 </p>
+
                <p>2µg/tube, 1 tube,Lyophilized </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Type of Strain Stock </p></td>
+
              <td valign="top"><p>Visual inspection </p></td>
+
              <td valign="top"><p>Bacteria stock. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Appearance </p></td>
+
              <td valign="top"><p>Visual inspection </p></td>
+
              <td valign="top"><p>Clear and free of foreign particles, and the strain is clearly visible. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
         
+
        </table>
+
        <p>  </p>
+
        <table width="519" border="1" cellpadding="0" cellspacing="0">
+
         
+
            <tr>
+
              <td width="175" valign="top"><p><img height="254.39999389648438" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/0LciDoc2Vues3GO6fYi7EnNqrsVS8mSF.qFDZziDpEA!/b/dMgAAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422"></p></td>
+
              <td width="194" valign="top"><p><img height="258.3999938964844" src="http://b198.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/hJNHsLw6HFbz9eR7eszEpW*U.RfWwyhYSlkMIPiWAbc!/b/dMYAAAAAAAAA&bo=wwADAQAAAAADAOQ!" width="194.39999389648438"></p></td>
+
              <td width="142" valign="top"><p>  </p>
+
                <p>Lane M: DNA Marker </p>
+
                <p>  </p>
+
                <p>Lane 1: Plasmid digested by XbaI and PstI </p>
+
                <p>  </p>
+
                <p>Lane 2: Plasmid DNA </p>
+
                <p>  </p>
+
                <p>  </p></td>
+
            </tr>
+
         
+
        </table>
+
= Cry11Aa4+Extended2A+Cyt1-pChlamy3 =
+
        <table width="519" border="1" cellpadding="0" cellspacing="0">
+
         
+
            <tr>
+
              <td width="118" valign="top"><p>Gene Name </p></td>
+
              <td width="395" valign="top"><p>Cry11Aa4+Extended2A+Cyt1-pChlamy3 </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Cloning Vector </p></td>
+
              <td valign="top"><p>pChlamy_3 </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Construct Resistant </p></td>
+
              <td valign="top"><p>Ampicillin in JM108 </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Gene Length </p></td>
+
              <td valign="top"><p>2820bp </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Cloning Strategy </p></td>
+
              <td valign="top"><p>XbaI and PstI </p></td>
+
            </tr>
+
         
+
        </table>
+
        <p>  </p>
+
        <table width="519" border="1" cellpadding="0" cellspacing="0">
+
         
+
            <tr>
+
              <td width="110" valign="top"><p>QC Items </p></td>
+
              <td width="125" valign="top"><p>Method </p></td>
+
              <td width="226" valign="top"><p>Specifications </p></td>
+
              <td width="48" valign="top"><p>Results </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Target Sequence </p></td>
+
              <td valign="top"><p>Sequence alignment </p></td>
+
              <td valign="top"><p>Sequencing results are consistent to the confirmed sequence. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Vector Sequence </p></td>
+
              <td valign="top"><p>Sequence alignment </p></td>
+
              <td valign="top"><p>20bp flanking sequences of the vector are correct. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Reading Frame </p></td>
+
              <td valign="top"><p>Sequence alignment </p></td>
+
              <td valign="top"><p>Frame is correct and consistent to the client's requirement. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Fragment Size </p></td>
+
              <td valign="top"><p>Restriction Digests </p></td>
+
              <td valign="top"><p>The size of inserted fragment is right and free of any contaminated bands. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Quality/Quantity </p></td>
+
              <td valign="top"><p>UV spectrophotometry </p></td>
+
              <td valign="top"><p>Miniprep: OD260/280=1.7~2.0 </p>
+
                <p>2µg/tube, 1 tube,Lyophilized </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Type of Strain Stock </p></td>
+
              <td valign="top"><p>Visual inspection </p></td>
+
              <td valign="top"><p>Bacteria stock. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Appearance </p></td>
+
              <td valign="top"><p>Visual inspection </p></td>
+
              <td valign="top"><p>Clear and free of foreign particles, and the strain is clearly visible. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
         
+
        </table>
+
        <p>  </p>
+
        <table width="519" border="1" cellpadding="0" cellspacing="0">
+
         
+
            <tr>
+
              <td width="175" valign="top"><p><img height="254.39999389648438" src="http://b264.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/.39If2pAeG1h2OXRhp49InOl3WTN1asUm8AXJFwwqZs!/b/dAgBAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422"></p></td>
+
              <td width="188" valign="top"><p><img height="264.0" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/pZ5hAlXd5CMg3MJ.c2OE2MHz2ke4Ev3fW3jyPfdOUPU!/b/dMgAAAAAAAAA&bo=vQAJAQAAAAADAJA!" width="188.0"></p></td>
+
              <td width="148" valign="top"><p>  </p>
+
                <p>Lane M: DNA Marker </p>
+
                <p>  </p>
+
                <p>Lane 1: Plasmid digested by XbaI and PstI </p>
+
                <p>  </p>
+
                <p>Lane 2: Plasmid DNA </p>
+
                <p>  </p>
+
                <p>  </p>
+
                <p>  </p></td>
+
            </tr>
+
         
+
        </table>
+
        <p>  </p>
+
= Cry11Aa4+Extended2A+EGFP-pChlamy3 =
+
<table width="519" border="1" cellpadding="0" cellspacing="0">
+
         
+
            <tr>
+
              <td width="94" valign="top"><p>Gene Name </p></td>
+
              <td width="419" valign="top"><p>Cry11Aa4+Extended2A+EGFP-pChlamy3 </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Cloning Vector </p></td>
+
              <td valign="top"><p>pChlamy_3 </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Construct Resistant </p></td>
+
              <td valign="top"><p>Ampicillin in JM108 </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Gene Length </p></td>
+
              <td valign="top"><p>2790bp </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Cloning Strategy </p></td>
+
              <td valign="top"><p>XbaI and PstI </p></td>
+
            </tr>
+
         
+
        </table>
+
        <p>  </p>
+
        <table width="519" border="1" cellpadding="0" cellspacing="0">
+
         
+
            <tr>
+
              <td width="110" valign="top"><p>QC Items </p></td>
+
              <td width="125" valign="top"><p>Method </p></td>
+
              <td width="226" valign="top"><p>Specifications </p></td>
+
              <td width="48" valign="top"><p>Results </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Target Sequence </p></td>
+
              <td valign="top"><p>Sequence alignment </p></td>
+
              <td valign="top"><p>Sequencing results are consistent to the confirmed sequence. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Vector Sequence </p></td>
+
              <td valign="top"><p>Sequence alignment </p></td>
+
              <td valign="top"><p>20bp flanking sequences of the vector are correct. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Reading Frame </p></td>
+
              <td valign="top"><p>Sequence alignment </p></td>
+
              <td valign="top"><p>Frame is correct and consistent to the client's requirement. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Fragment Size </p></td>
+
              <td valign="top"><p>Restriction Digests </p></td>
+
              <td valign="top"><p>The size of inserted fragment is right and free of any contaminated bands. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Quality/Quantity </p></td>
+
              <td valign="top"><p>UV spectrophotometry </p></td>
+
              <td valign="top"><p>Miniprep: OD260/280=1.7~2.0 </p>
+
                <p>2µg/tube, 1 tube,Lyophilized </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Type of Strain Stock </p></td>
+
              <td valign="top"><p>Visual inspection </p></td>
+
              <td valign="top"><p>Bacteria stock. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
            <tr>
+
              <td valign="top"><p>Appearance </p></td>
+
              <td valign="top"><p>Visual inspection </p></td>
+
              <td valign="top"><p>Clear and free of foreign particles, and the strain is clearly visible. </p></td>
+
              <td valign="top"><p>Pass </p></td>
+
            </tr>
+
         
+
        </table>
+
        <p>  </p>
+
        <table width="519" border="1" cellpadding="0" cellspacing="0">
+
         
+
            <tr>
+
              <td width="175" valign="top"><p><img height="254.39999389648438" src="http://b262.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/cI8hqU6TnQQhJWgxrwgmQv0Mwgv4T3KwluBqsv0K9KA!/b/dAYBAAAAAAAA&bo=sAD*AAAAAAADAGo!" width="175.1999969482422"></p></td>
+
              <td width="168" valign="top"><p><img height="259.20001220703125" src="http://b198.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/94oeXP5iFtJKrKNWD4RQCmRJXVM2sO.rQztD9R2O50w!/b/dMYAAAAAAAAA&bo=qQAEAQAAAAADAIk!" width="168.8000030517578"></p></td>
+
              <td width="168" valign="top"><p>  </p>
+
                <p>Lane M: DNA Marker </p>
+
                <p>  </p>
+
                <p>Lane 1: Plasmid digested by XbaI and PstI </p>
+
                <p>  </p>
+
                <p>Lane 2: Plasmid DNA </p>
+
                <p>  </p>
+
                <p>  </p>
+
                <p>  </p></td>
+
            </tr>
+
         
+
        </table>
+
        <p> </p>
+
      </div>
+
    </div>
+
  </div>
+
 
</div>
 
</div>
 +
= <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:16.0pt;font-family:&quot;:,sans-serif;color:#1E4E79">Cry4Aa4+Extended FMDV 2A-pChlamy_3</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> =
 +
<table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse">
 +
<tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Gene Name</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Cry4Aa4+Extended FMDV 2A</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Cloning Vector</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">pChlamy_3</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Construct Resistant</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Ampicillin in DH5α</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Gene Length</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">3673bp</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Cloning Strategy</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">XbaI and PstI</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr>
 +
</table>
 +
<p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
 +
<table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse">
 +
<tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">QC Items</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Method</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Specifications</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Results</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Target Sequence</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequencing results are consistent to  the confirmed sequence.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Vector Sequence</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">20bp flanking sequences of the  vector are correct.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Reading Frame</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Frame is correct and consistent to the  client's requirement.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Fragment Size</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Restriction Digests</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">The size of inserted fragment is  right and free of any contaminated bands.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Quality/Quantity</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">UV spectrophotometry</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Miniprep: OD260/280=1.7~2.0</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">2µg/tube, 1 tube,Lyophilized</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Type of Strain Stock</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Visual inspection</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Bacteria stock.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Appearance</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Visual inspection</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Clear and free of foreign particles,  and the strain is clearly visible.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr>
 +
</table>
 +
<p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
 +
<table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse">
 +
<tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:12pt;font-family:宋体">                                                                      [[File:T--FAFU-CHINA--bs1.png | 825px | thumb | center ]]</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:12pt;font-family:宋体">    [[File:T--FAFU-CHINA--bs2.png | 825px | thumb | center ]]</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane M: DNA Marker</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane 1: Plasmid digested by XbaI and  PstI</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane 2: Plasmid DNA</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr>
 +
</table>
 +
<p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:16.0pt;font-family:&quot;:,sans-serif;color:#1E4E79">&nbsp;</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
<p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:16.0pt;font-family:&quot;:,sans-serif;color:#1E4E79">&nbsp;</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
= <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:16.0pt;font-family:&quot;:,sans-serif;color:#1E4E79">Cry10Aa4+Extended FMDV 2A-pChlamy_3</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> =
 +
<table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse">
 +
<tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Gene Name</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> 
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Cloning Vector</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">pChlamy_3</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Construct Resistant</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Ampicillin in Top10</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Gene Length</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">2158bp</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Cloning Strategy</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">XbaI and PstI</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr>
 +
</table>
 +
<p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
 +
<table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse">
 +
<tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">QC Items</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Method</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Specifications</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Results</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Target Sequence</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequencing results are consistent to  the confirmed sequence.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Vector Sequence</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">20bp flanking sequences of the  vector are correct.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Reading Frame</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Frame is correct and consistent to  the client's requirement.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Fragment Size</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Restriction Digests</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">The size of inserted fragment is  right and free of any contaminated bands.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Quality/Quantity</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">UV spectrophotometry</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Miniprep: OD260/280=1.7~2.0</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">2µg/tube, 1 tube,Lyophilized</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Type of Strain Stock</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Visual inspection</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Bacteria stock.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Appearance</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Visual inspection</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Clear and free of foreign particles,  and the strain is clearly visible.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr>
 +
</table>
 +
<p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
 +
<table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse">
 +
<tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:12pt;font-family:宋体">    [[File:T--FAFU-CHINA--bs3.png | 825px | thumb | center ]]</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:12pt;font-family:宋体">    [[File:T--FAFU-CHINA--bs4.png | 825px | thumb | center ]]</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane M: DNA Marker</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane 1: Plasmid digested by XbaI and  PstI</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane 2: Plasmid DNA</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr>
 +
</table>
 +
<p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:16.0pt;font-family:&quot;:,sans-serif;color:#1E4E79">&nbsp;</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
<p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:16.0pt;font-family:&quot;:,sans-serif;color:#1E4E79">&nbsp;</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
= <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:16.0pt;font-family:&quot;:,sans-serif;color:#1E4E79">Cry11Aa4+Extended FMDV 2A-pChlamy_3</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> =
 +
 +
<table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse">
 +
<tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Gene Name</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Cry11Aa4+ Extended FMDV 2A</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Cloning Vector</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">pChlamy_3</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Construct Resistant</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Ampicillin in JM108</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Gene Length</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">2071bp</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Cloning Strategy</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">XbaI and PstI</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr>
 +
</table>
 +
<p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
 +
<table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse">
 +
<tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">QC Items</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Method</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Specifications</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Results</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Target Sequence</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequencing results are consistent to  the confirmed sequence.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Vector Sequence</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">20bp flanking sequences of the  vector are correct.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Reading Frame</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Frame is correct and consistent to  the client's requirement.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Fragment Size</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Restriction Digests</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">The size of inserted fragment is  right and free of any contaminated bands.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Quality/Quantity</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">UV spectrophotometry</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Miniprep: OD260/280=1.7~2.0</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">2µg/tube, 1 tube,Lyophilized</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Type of Strain Stock</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Visual inspection</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Bacteria stock.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Appearance</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Visual inspection</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Clear and free of foreign particles,  and the strain is clearly visible.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr>
 +
</table>
 +
<p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
 +
<table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse">
 +
<tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:12pt;font-family:宋体">    [[File:T--FAFU-CHINA--bs3.png | 825px | thumb | center ]]</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:12pt;font-family:宋体">    [[File:T--FAFU-CHINA--bs6.png | 825px | thumb | center ]]</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane M: DNA Marker</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane 1: Plasmid digested by XbaI and  PstI</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane 2: Plasmid DNA</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><i><span style="font-size:9.0pt;font-family:&quot;:,sans-serif;color:#595959">&nbsp;</span></i><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr>
 +
</table>
 +
 +
<p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:16.0pt;font-family:&quot;:,sans-serif;color:#1E4E79">&nbsp;</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
= <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:16.0pt;font-family:&quot;:,sans-serif;color:#1E4E79">Cyt1-pChlamy_3</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> =
 +
<table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse">
 +
<tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Gene Name</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Cyt1</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Cloning Vector</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">pChlamy_3</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Construct Resistant</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Ampicillin in JM108</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Gene Length</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">762bp</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Cloning Strategy</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">XbaI and PstI</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr>
 +
</table>
 +
<p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
 +
<table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse">
 +
<tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">QC Items</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Method</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Specifications</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Results</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Target Sequence</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequencing results are consistent to  the confirmed sequence.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Vector Sequence</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">20bp flanking sequences of the  vector are correct.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Reading Frame</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Frame is correct and consistent to  the client's requirement.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Fragment Size</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Restriction Digests</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">The size of inserted fragment is  right and free of any contaminated bands.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Quality/Quantity</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">UV spectrophotometry</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Miniprep: OD260/280=1.7~2.0</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">2µg/tube, 1 tube,Lyophilized</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Type of Strain Stock</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Visual inspection</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Bacteria stock.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Appearance</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Visual inspection</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Clear and free of foreign particles,  and the strain is clearly visible.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr>
 +
</table>
 +
<p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
 +
<table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse">
 +
<tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:12pt;font-family:宋体">    [[File:T--FAFU-CHINA--bs3.png | 825px | thumb | center ]]</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:12pt;font-family:宋体">    [[File:T--FAFU-CHINA--bs8.png | 825px | thumb | center ]]</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane M: DNA Marker</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane 1: Plasmid digested by XbaI and  PstI</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane 2: Plasmid DNA</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><i><span style="font-size:9.0pt;font-family:&quot;:,sans-serif;color:#595959">&nbsp;</span></i><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr>
 +
</table>
 +
<p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:16.0pt;font-family:&quot;:,sans-serif;color:#1E4E79">&nbsp;</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
<p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:16.0pt;font-family:&quot;:,sans-serif;color:#1E4E79">&nbsp;</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
= <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:16.0pt;font-family:&quot;:,sans-serif;color:#1E4E79">Cyt2-pChlamy_3</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> =
 +
 +
<table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse">
 +
<tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Gene Name</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Cyt1</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Cloning Vector</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">pChlamy_3</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Construct Resistant</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Ampicillin in JM108</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Gene Length</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">801bp</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Cloning Strategy</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">XbaI and PstI</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr>
 +
</table>
 +
<p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
 +
<table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse">
 +
<tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">QC Items</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Method</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Specifications</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Results</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Target Sequence</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequencing results are consistent to  the confirmed sequence.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Vector Sequence</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">20bp flanking sequences of the  vector are correct.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Reading Frame</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Frame is correct and consistent to  the client's requirement.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Fragment Size</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Restriction Digests</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">The size of inserted fragment is  right and free of any contaminated bands.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Quality/Quantity</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">UV spectrophotometry</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Miniprep: OD260/280=1.7~2.0</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">2µg/tube, 1 tube,Lyophilized</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Type of Strain Stock</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Visual inspection</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Bacteria stock.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Appearance</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Visual inspection</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Clear and free of foreign particles,  and the strain is clearly visible.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td colspan="2" valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:12pt;font-family:宋体">    [[File:T--FAFU-CHINA--bs3.png | 825px | thumb | center ]]</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:12pt;font-family:宋体">    [[File:T--FAFU-CHINA--bs10.png | 825px | thumb | center ]]</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane M: DNA Marker</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane 1: Plasmid digested by XbaI and  PstI</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane 2: Plasmid DNA</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td width="54" valign="top" style="width:40.5pt;padding:0cm 0cm 0cm 0cm">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Tahoma, sans-serif">&nbsp;</span></p>
 +
  </td> </tr> <tr>  <td width="138" valign="top" style="width:103.5pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"></td>  <td width="45" valign="top" style="width:33.75pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"></td>  <td width="124" valign="top" style="width:93.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"></td>  <td width="107" valign="top" style="width:80.25pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"></td>  <td width="80" valign="top" style="width:60.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"></td>  <td width="2" valign="top" style="width:1.5pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"></td>  <td width="54" valign="top" style="width:40.5pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"></td> </tr>
 +
</table>
 +
<p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:16.0pt;font-family:&quot;:,sans-serif;color:#1E4E79">&nbsp;</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
<p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:16.0pt;font-family:&quot;:,sans-serif;color:#1E4E79">&nbsp;</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
<p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:16.0pt;font-family:&quot;:,sans-serif;color:#1E4E79">&nbsp;</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
= <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:16.0pt;font-family:&quot;:,sans-serif;color:#1E4E79">EGFP-pChlamy_3</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> =
 +
 +
<table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse">
 +
<tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Gene Name</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">06-EGFP</span><span style="font-size:11pt;font-family:宋体">(</span><span style="font-size:11pt;font-family:Calibri, sans-serif">TAA</span><span style="font-size:11pt;font-family:宋体">)</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Cloning Vector</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">pChlamy_3</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Construct Resistant</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Ampicillin in Top10</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Gene Length</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">732bp</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Cloning Strategy</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">XbaI and PstI</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr>
 +
</table>
 +
<p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
 +
<table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse">
 +
<tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">QC Items</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Method</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Specifications</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Results</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Target Sequence</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequencing results are consistent to  the confirmed sequence.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Vector Sequence</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">20bp flanking sequences of the  vector are correct.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Reading Frame</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Frame is correct and consistent to  the client's requirement.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Fragment Size</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Restriction Digests</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">The size of inserted fragment is  right and free of any contaminated bands.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Quality/Quantity</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">UV spectrophotometry</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Miniprep: OD260/280=1.7~2.0</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">2µg/tube, 1 tube,Lyophilized</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Type of Strain Stock</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Visual inspection</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Bacteria stock.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Appearance</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Visual inspection</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Clear and free of foreign particles,  and the strain is clearly visible.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr>
 +
</table>
 +
<p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
 +
<table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse">
 +
<tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:12pt;font-family:宋体">    [[File:T--FAFU-CHINA--bs3.png | 825px | thumb | center ]]</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:12pt;font-family:宋体">    [[File:T--FAFU-CHINA--bs12.png | 825px | thumb | center ]]</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane M: DNA Marker</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane 1: Plasmid digested by XbaI and  PstI</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane 2: Plasmid DNA</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr>
 +
</table>
 +
<p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:16.0pt;font-family:&quot;:,sans-serif;color:#1E4E79">&nbsp;</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
  
 
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Latest revision as of 06:36, 8 November 2016

Team FAFU-CHINA

 Basic Part

Cry4Aa4+Extended FMDV 2A-pChlamy_3

Gene Name

Cry4Aa4+Extended FMDV 2A

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in DH5α

Gene Length

3673bp

Cloning Strategy

XbaI and PstI

 

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

 

T--FAFU-CHINA--bs1.png

 

T--FAFU-CHINA--bs2.png

 

Lane M: DNA Marker

 

Lane 1: Plasmid digested by XbaI and PstI

 

Lane 2: Plasmid DNA

 

 

Cry10Aa4+Extended FMDV 2A-pChlamy_3

Gene Name

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in Top10

Gene Length

2158bp

Cloning Strategy

XbaI and PstI

 

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

 

T--FAFU-CHINA--bs3.png

 

T--FAFU-CHINA--bs4.png

 

Lane M: DNA Marker

 

Lane 1: Plasmid digested by XbaI and PstI

 

Lane 2: Plasmid DNA

 

 

Cry11Aa4+Extended FMDV 2A-pChlamy_3

Gene Name

Cry11Aa4+ Extended FMDV 2A

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in JM108

Gene Length

2071bp

Cloning Strategy

XbaI and PstI

 

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

 

T--FAFU-CHINA--bs3.png

 

T--FAFU-CHINA--bs6.png

Lane M: DNA Marker

 

Lane 1: Plasmid digested by XbaI and PstI

 

Lane 2: Plasmid DNA

 

 

Cyt1-pChlamy_3

Gene Name

Cyt1

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in JM108

Gene Length

762bp

Cloning Strategy

XbaI and PstI

 

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

 

T--FAFU-CHINA--bs3.png

 

T--FAFU-CHINA--bs8.png

 

Lane M: DNA Marker

 

Lane 1: Plasmid digested by XbaI and PstI

 

Lane 2: Plasmid DNA

 

 

 

 

Cyt2-pChlamy_3

Gene Name

Cyt1

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in JM108

Gene Length

801bp

Cloning Strategy

XbaI and PstI

 

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

T--FAFU-CHINA--bs3.png

 

T--FAFU-CHINA--bs10.png

 

Lane M: DNA Marker

 

Lane 1: Plasmid digested by XbaI and PstI

 

Lane 2: Plasmid DNA

 

 

 

 

 

 

EGFP-pChlamy_3

Gene Name

06-EGFPTAA

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in Top10

Gene Length

732bp

Cloning Strategy

XbaI and PstI

 

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

 

T--FAFU-CHINA--bs3.png

T--FAFU-CHINA--bs12.png

 

Lane M: DNA Marker

 

Lane 1: Plasmid digested by XbaI and PstI

 

Lane 2: Plasmid DNA