Difference between revisions of "Team:FAFU-CHINA/Basic Part"

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{{FAFU-CHINA}}
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{{:Team:FAFU-CHINA/menu2}}
 
<html>
 
<html>
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  <div id="main-content" class="container">
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    <div class="col-md-3" id="document-sidebar">
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</html>
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__TOC__
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__NOEDITSECTION__
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<html>
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    </div>
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  <script>
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        $("#toctitle").remove();
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var toc_tbl = $("#toc")
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    .prop("id","table-contents")
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    .addClass("panel panel-primary")
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    .removeClass("toc")
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    .children()
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      .clone();
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$("#document-sidebar")
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.children()
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.remove()
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.end()
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.html('<div class="bs-docs-sidebar hidden-print hidden-xs hidden-sm" id="page-contents"></div>');
  
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<div class="column full_size judges-will-not-evaluate">
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toc_tbl.appendTo("#page-contents");
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<h3>★  ALERT! </h3>
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        $("#page-contents ul").addClass("nav");
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<p>This page is used by the judges to evaluate your team for the <a href="https://2016.igem.org/Judging/Awards#Special_Prizes">basic part special prize</a>. </p>
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        $("#page-contents > ul").addClass("bs-docs-sidenav");
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        $("#toctitle").remove();
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        $(".tocnumber").remove();
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  </script>
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    <div class="col-md-9" id="document-body">
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</html>
  
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    <!-- theme -->
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<html>
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<head>
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  <meta charset="utf-8">
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  <meta http-equiv="X-UA-Compatible" content="IE=edge">
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  <meta name="viewport" content="width=device-width, initial-scale=1,maximum-scale=1.0,user-scalable=no">
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  <meta name="description" content="">
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  <meta name="author" content="">
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  <meta http-equiv="X-UA-Compatible" content="IE=edge" />
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  <link rel="shortcut icon" href="/favicon.ico">
  
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  <title>Team FAFU-CHINA</title>
  
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<p> Delete this box in order to be evaluated for this medal. See more information at <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions"> Instructions for Pages for awards</a>.</p>
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  <!-- Bootstrap core CSS -->
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  <link rel="stylesheet" href="https://2016.igem.org/Team:FAFU-CHINA/page/css/toolbar?action=raw&ctype=text/css">
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  <link rel="stylesheet" href="https://2016.igem.org/Team:FAFU-CHINA/page/css/cstyle?action=raw&ctype=text/css">
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  body {
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.bs-docs-header #title-icon{
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.team-content h2{
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.team-content .team-member h2{
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.team-content .team-member h3{
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.team-content .team-member p{
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}
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.team-content .team-member img{
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    width: 100%;
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    max-width: 300px;
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    margin-top: 25px;
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}
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.team-content .group-photo img{
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    width: 100%;
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    max-width: 1000px;
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    margin-left: auto;
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    display: block;
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    margin-top: 25px;
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    margin-bottom: 25px;
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}
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.svg {
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    width:100%;
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    height:300px;
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}
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  #content {
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    margin-top: 70px;
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    font-family: Lato, inherit;
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  }
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.footer img {
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    width: 100%;
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    max-width: 200px;
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    display: block;
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    margin-left: auto;
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    margin-right: auto;
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}
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  </style>
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  <!-- HTML5 shim and Respond.js IE8 support of HTML5 elements and media queries -->
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  <!--[if lt IE 9]>
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      <script src="https://2016.igem.org/Team:FAFU-CHINA/page/css/html5shiv?action=raw&ctype=text/javascrip"></script>
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      <script src="https://2016.igem.org/Team:FAFU-CHINA/page/css/respond?action=raw&ctype=text/javascrip"></script>
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    <![endif]-->
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</head>
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</html>
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    <!-- theme -->
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    <!-- word begin-->
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<div>&nbsp;<b><span style="font-size:11pt;font-family:Arial, sans-serif">Basic Part</span></b><br/>
 
</div>
 
</div>
 +
= <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:16.0pt;font-family:&quot;:,sans-serif;color:#1E4E79">Cry4Aa4+Extended FMDV 2A-pChlamy_3</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> =
 +
<table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse">
 +
<tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Gene Name</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Cry4Aa4+Extended FMDV 2A</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Cloning Vector</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">pChlamy_3</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Construct Resistant</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Ampicillin in DH5α</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Gene Length</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">3673bp</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Cloning Strategy</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">XbaI and PstI</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr>
 +
</table>
 +
<p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
  
 +
<table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse">
 +
<tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">QC Items</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Method</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Specifications</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Results</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Target Sequence</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequencing results are consistent to  the confirmed sequence.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Vector Sequence</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">20bp flanking sequences of the  vector are correct.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Reading Frame</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Frame is correct and consistent to the  client's requirement.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Fragment Size</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Restriction Digests</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">The size of inserted fragment is  right and free of any contaminated bands.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Quality/Quantity</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">UV spectrophotometry</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Miniprep: OD260/280=1.7~2.0</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">2µg/tube, 1 tube,Lyophilized</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Type of Strain Stock</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Visual inspection</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Bacteria stock.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Appearance</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Visual inspection</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Clear and free of foreign particles,  and the strain is clearly visible.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr>
 +
</table>
 +
<p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
  
 +
<table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse">
 +
<tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:12pt;font-family:宋体">                                                                      [[File:T--FAFU-CHINA--bs1.png | 825px | thumb | center ]]</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:12pt;font-family:宋体">    [[File:T--FAFU-CHINA--bs2.png | 825px | thumb | center ]]</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane M: DNA Marker</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane 1: Plasmid digested by XbaI and  PstI</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane 2: Plasmid DNA</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr>
 +
</table>
 +
<p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:16.0pt;font-family:&quot;:,sans-serif;color:#1E4E79">&nbsp;</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
<p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:16.0pt;font-family:&quot;:,sans-serif;color:#1E4E79">&nbsp;</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
= <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:16.0pt;font-family:&quot;:,sans-serif;color:#1E4E79">Cry10Aa4+Extended FMDV 2A-pChlamy_3</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> =
 +
<table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse">
 +
<tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Gene Name</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt"> 
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Cloning Vector</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">pChlamy_3</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Construct Resistant</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Ampicillin in Top10</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Gene Length</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">2158bp</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Cloning Strategy</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">XbaI and PstI</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr>
 +
</table>
 +
<p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
  
 +
<table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse">
 +
<tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">QC Items</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Method</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Specifications</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Results</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Target Sequence</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequencing results are consistent to  the confirmed sequence.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Vector Sequence</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">20bp flanking sequences of the  vector are correct.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Reading Frame</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Frame is correct and consistent to  the client's requirement.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Fragment Size</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Restriction Digests</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">The size of inserted fragment is  right and free of any contaminated bands.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Quality/Quantity</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">UV spectrophotometry</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Miniprep: OD260/280=1.7~2.0</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">2µg/tube, 1 tube,Lyophilized</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Type of Strain Stock</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Visual inspection</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Bacteria stock.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Appearance</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Visual inspection</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Clear and free of foreign particles,  and the strain is clearly visible.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr>
 +
</table>
 +
<p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
  
−
<div class="column full_width">
+
<table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse">
 +
<tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:12pt;font-family:宋体">    [[File:T--FAFU-CHINA--bs3.png | 825px | thumb | center ]]</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:12pt;font-family:宋体">    [[File:T--FAFU-CHINA--bs4.png | 825px | thumb | center ]]</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane M: DNA Marker</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane 1: Plasmid digested by XbaI and  PstI</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane 2: Plasmid DNA</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr>
 +
</table>
 +
<p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:16.0pt;font-family:&quot;:,sans-serif;color:#1E4E79">&nbsp;</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
<p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:16.0pt;font-family:&quot;:,sans-serif;color:#1E4E79">&nbsp;</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
= <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:16.0pt;font-family:&quot;:,sans-serif;color:#1E4E79">Cry11Aa4+Extended FMDV 2A-pChlamy_3</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> =
  
 +
<table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse">
 +
<tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Gene Name</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Cry11Aa4+ Extended FMDV 2A</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Cloning Vector</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">pChlamy_3</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Construct Resistant</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Ampicillin in JM108</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Gene Length</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">2071bp</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Cloning Strategy</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">XbaI and PstI</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr>
 +
</table>
 +
<p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
  
 +
<table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse">
 +
<tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">QC Items</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Method</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Specifications</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Results</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Target Sequence</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequencing results are consistent to  the confirmed sequence.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Vector Sequence</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">20bp flanking sequences of the  vector are correct.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Reading Frame</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Frame is correct and consistent to  the client's requirement.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Fragment Size</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Restriction Digests</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">The size of inserted fragment is  right and free of any contaminated bands.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Quality/Quantity</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">UV spectrophotometry</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Miniprep: OD260/280=1.7~2.0</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">2µg/tube, 1 tube,Lyophilized</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Type of Strain Stock</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Visual inspection</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Bacteria stock.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Appearance</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Visual inspection</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Clear and free of foreign particles,  and the strain is clearly visible.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr>
 +
</table>
 +
<p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
  
 +
<table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse">
 +
<tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:12pt;font-family:宋体">    [[File:T--FAFU-CHINA--bs3.png | 825px | thumb | center ]]</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:12pt;font-family:宋体">    [[File:T--FAFU-CHINA--bs6.png | 825px | thumb | center ]]</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane M: DNA Marker</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane 1: Plasmid digested by XbaI and  PstI</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane 2: Plasmid DNA</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><i><span style="font-size:9.0pt;font-family:&quot;:,sans-serif;color:#595959">&nbsp;</span></i><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr>
 +
</table>
  
−
<p>
+
<p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:16.0pt;font-family:&quot;:,sans-serif;color:#1E4E79">&nbsp;</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
−
A <b>basic part</b> is a functional unit of DNA that cannot be subdivided into smaller component parts. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_R0051">BBa_R0051</a> is an example of a basic part, a promoter regulated by lambda cl.
+
= <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:16.0pt;font-family:&quot;:,sans-serif;color:#1E4E79">Cyt1-pChlamy_3</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> =
−
</p>
+
<table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse">
 +
<tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Gene Name</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Cyt1</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Cloning Vector</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">pChlamy_3</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Construct Resistant</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Ampicillin in JM108</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Gene Length</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">762bp</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Cloning Strategy</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">XbaI and PstI</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr>
 +
</table>
 +
<p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
  
−
<p>Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are <b>many</b> opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts. </p>
+
<table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse">
 +
<tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">QC Items</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Method</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Specifications</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Results</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Target Sequence</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequencing results are consistent to the confirmed sequence.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Vector Sequence</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">20bp flanking sequences of the  vector are correct.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Reading Frame</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Frame is correct and consistent to  the client's requirement.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Fragment Size</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Restriction Digests</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">The size of inserted fragment is  right and free of any contaminated bands.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Quality/Quantity</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">UV spectrophotometry</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Miniprep: OD260/280=1.7~2.0</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">2µg/tube, 1 tube,Lyophilized</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Type of Strain Stock</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Visual inspection</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Bacteria stock.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Appearance</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Visual inspection</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Clear and free of foreign particles,  and the strain is clearly visible.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr>
 +
</table>
 +
<p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
  
 +
<table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse">
 +
<tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:12pt;font-family:宋体">    [[File:T--FAFU-CHINA--bs3.png | 825px | thumb | center ]]</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:12pt;font-family:宋体">    [[File:T--FAFU-CHINA--bs8.png | 825px | thumb | center ]]</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane M: DNA Marker</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane 1: Plasmid digested by XbaI and  PstI</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane 2: Plasmid DNA</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><i><span style="font-size:9.0pt;font-family:&quot;:,sans-serif;color:#595959">&nbsp;</span></i><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr>
 +
</table>
 +
<p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:16.0pt;font-family:&quot;:,sans-serif;color:#1E4E79">&nbsp;</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
<p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:16.0pt;font-family:&quot;:,sans-serif;color:#1E4E79">&nbsp;</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
= <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:16.0pt;font-family:&quot;:,sans-serif;color:#1E4E79">Cyt2-pChlamy_3</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> =
  
 +
<table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse">
 +
<tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Gene Name</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Cyt1</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Cloning Vector</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">pChlamy_3</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Construct Resistant</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Ampicillin in JM108</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Gene Length</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">801bp</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Cloning Strategy</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">XbaI and PstI</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr>
 +
</table>
 +
<p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
  
 +
<table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse">
 +
<tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">QC Items</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Method</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Specifications</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Results</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Target Sequence</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequencing results are consistent to  the confirmed sequence.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Vector Sequence</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">20bp flanking sequences of the  vector are correct.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Reading Frame</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Frame is correct and consistent to  the client's requirement.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Fragment Size</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Restriction Digests</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">The size of inserted fragment is  right and free of any contaminated bands.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Quality/Quantity</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">UV spectrophotometry</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Miniprep: OD260/280=1.7~2.0</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">2µg/tube, 1 tube,Lyophilized</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Type of Strain Stock</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Visual inspection</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Bacteria stock.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Appearance</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Visual inspection</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Clear and free of foreign particles,  and the strain is clearly visible.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td colspan="2" valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:12pt;font-family:宋体">    [[File:T--FAFU-CHINA--bs3.png | 825px | thumb | center ]]</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:12pt;font-family:宋体">    [[File:T--FAFU-CHINA--bs10.png | 825px | thumb | center ]]</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td colspan="2" valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane M: DNA Marker</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane 1: Plasmid digested by XbaI and  PstI</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane 2: Plasmid DNA</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td width="54" valign="top" style="width:40.5pt;padding:0cm 0cm 0cm 0cm">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Tahoma, sans-serif">&nbsp;</span></p>
 +
  </td> </tr> <tr>  <td width="138" valign="top" style="width:103.5pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"></td>  <td width="45" valign="top" style="width:33.75pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"></td>  <td width="124" valign="top" style="width:93.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"></td>  <td width="107" valign="top" style="width:80.25pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"></td>  <td width="80" valign="top" style="width:60.0pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"></td>  <td width="2" valign="top" style="width:1.5pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"></td>  <td width="54" valign="top" style="width:40.5pt;padding:7.5pt 7.5pt 7.5pt 7.5pt"></td> </tr>
 +
</table>
 +
<p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:16.0pt;font-family:&quot;:,sans-serif;color:#1E4E79">&nbsp;</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
<p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:16.0pt;font-family:&quot;:,sans-serif;color:#1E4E79">&nbsp;</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
<p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:16.0pt;font-family:&quot;:,sans-serif;color:#1E4E79">&nbsp;</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
= <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:16.0pt;font-family:&quot;:,sans-serif;color:#1E4E79">EGFP-pChlamy_3</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> =
  
 +
<table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse">
 +
<tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Gene Name</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">06-EGFP</span><span style="font-size:11pt;font-family:宋体">（</span><span style="font-size:11pt;font-family:Calibri, sans-serif">TAA</span><span style="font-size:11pt;font-family:宋体">）</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Cloning Vector</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">pChlamy_3</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Construct Resistant</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Ampicillin in Top10</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Gene Length</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">732bp</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Cloning Strategy</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">XbaI and PstI</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr>
 +
</table>
 +
<p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
  
−
<div class="highlight">
+
<table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse">
−
<h4>Note</h4>
+
<tr> <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">QC Items</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
−
<p>This page should list all the basic parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page.</p>
+
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Method</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Specifications</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Results</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Target Sequence</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequencing results are consistent to  the confirmed sequence.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Vector Sequence</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">20bp flanking sequences of the vector are correct.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Reading Frame</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Frame is correct and consistent to  the client's requirement.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Fragment Size</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Restriction Digests</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">The size of inserted fragment is  right and free of any contaminated bands.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Quality/Quantity</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">UV spectrophotometry</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Miniprep: OD260/280=1.7~2.0</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">2µg/tube, 1 tube,Lyophilized</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Type of Strain Stock</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Visual inspection</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Bacteria stock.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Appearance</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Visual inspection</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Clear and free of foreign particles,  and the strain is clearly visible.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr>
 +
</table>
 +
<p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
  
 +
<table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse">
 +
<tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:12pt;font-family:宋体">    [[File:T--FAFU-CHINA--bs3.png | 825px | thumb | center ]]</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:12pt;font-family:宋体">    [[File:T--FAFU-CHINA--bs12.png | 825px | thumb | center ]]</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane M: DNA Marker</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane 1: Plasmid digested by XbaI and  PstI</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane 2: Plasmid DNA</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr>
 +
</table>
 +
<p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:16.0pt;font-family:&quot;:,sans-serif;color:#1E4E79">&nbsp;</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
  
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Latest revision as of 06:36, 8 November 2016

Team FAFU-CHINA

 Basic Part

Cry4Aa4+Extended FMDV 2A-pChlamy_3

Gene Name

Cry4Aa4+Extended FMDV 2A

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in DH5α

Gene Length

3673bp

Cloning Strategy

XbaI and PstI

 

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

 

T--FAFU-CHINA--bs1.png

 

T--FAFU-CHINA--bs2.png

 

Lane M: DNA Marker

 

Lane 1: Plasmid digested by XbaI and PstI

 

Lane 2: Plasmid DNA

 

 

Cry10Aa4+Extended FMDV 2A-pChlamy_3

Gene Name

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in Top10

Gene Length

2158bp

Cloning Strategy

XbaI and PstI

 

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

 

T--FAFU-CHINA--bs3.png

 

T--FAFU-CHINA--bs4.png

 

Lane M: DNA Marker

 

Lane 1: Plasmid digested by XbaI and PstI

 

Lane 2: Plasmid DNA

 

 

Cry11Aa4+Extended FMDV 2A-pChlamy_3

Gene Name

Cry11Aa4+ Extended FMDV 2A

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in JM108

Gene Length

2071bp

Cloning Strategy

XbaI and PstI

 

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

 

T--FAFU-CHINA--bs3.png

 

T--FAFU-CHINA--bs6.png

Lane M: DNA Marker

 

Lane 1: Plasmid digested by XbaI and PstI

 

Lane 2: Plasmid DNA

 

 

Cyt1-pChlamy_3

Gene Name

Cyt1

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in JM108

Gene Length

762bp

Cloning Strategy

XbaI and PstI

 

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

 

T--FAFU-CHINA--bs3.png

 

T--FAFU-CHINA--bs8.png

 

Lane M: DNA Marker

 

Lane 1: Plasmid digested by XbaI and PstI

 

Lane 2: Plasmid DNA

 

 

 

 

Cyt2-pChlamy_3

Gene Name

Cyt1

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in JM108

Gene Length

801bp

Cloning Strategy

XbaI and PstI

 

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

T--FAFU-CHINA--bs3.png

 

T--FAFU-CHINA--bs10.png

 

Lane M: DNA Marker

 

Lane 1: Plasmid digested by XbaI and PstI

 

Lane 2: Plasmid DNA

 

 

 

 

 

 

EGFP-pChlamy_3

Gene Name

06-EGFP（TAA）

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in Top10

Gene Length

732bp

Cloning Strategy

XbaI and PstI

 

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

 

T--FAFU-CHINA--bs3.png

T--FAFU-CHINA--bs12.png

 

Lane M: DNA Marker

 

Lane 1: Plasmid digested by XbaI and PstI

 

Lane 2: Plasmid DNA