Difference between revisions of "Team:FAFU-CHINA/Model/Growth Model"

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<div style="height:auto!important;margin-left:auto;margin-right:auto;min-height:841.9pt;padding-bottom:1pt;padding-top:1pt">     
 
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<div style="margin-left:auto;margin-right:auto;position:relative"><p style="text-align:center"><a name="_GoBack"></a><span><span style="font-family:Times New Roman;font-size:12.0pt">The Improvement of Parts<span></span></span></span></p>
 
<p style="text-align:left;text-indent:21.0pt"><span><span style="font-family:Times New Roman;font-size:12.0pt">When we made the draft plan about our project, we noticed that NCTU_Formosa 2010’s project made use of </span></span><span><span style="font-family:Times New Roman;font-size:12.0pt;font-style:italic">Cry11Aa</span></span><span><span style="font-family:Times New Roman;font-size:12.0pt"> gene. And they constructed the related part BBa_K332011. In their project, they transformed the Cry11Aa gene into </span></span><span><span style="font-family:Times New Roman;font-size:12.0pt;font-style:italic">E. coli</span></span><span><span style="font-family:Times New Roman;font-size:12.0pt"> to kill the larvae of mosquitoes. Considering the potential safety problem, using </span></span><span><span style="font-family:Times New Roman;font-size:12.0pt;font-style:italic">E. coli </span></span><span><span style="font-family:Times New Roman;font-size:12.0pt">in the natural environment is not a good idea. Therefore, we decided to use </span></span><span><span style="font-family:Times New Roman;font-size:12.0pt;font-style:italic">Chlamydomonas reinhardti </span></span><span><span style="font-family:Times New Roman;font-size:12.0pt">to express toxin genes such as </span></span><span><span style="font-family:Times New Roman;font-size:12.0pt;font-style:italic">Cry11Aa</span></span><span><span style="font-family:Times New Roman;font-size:12.0pt">. Firstly, we wanted to ask them to send this part to us. And we can transform it to </span></span><span><span style="font-family:Times New Roman;font-size:12.0pt;font-style:italic">Chlamydomonas reinhardti </span></span><span><span style="font-family:Times New Roman;font-size:12.0pt">to test our idea. But we noticed that the codon bias of </span></span><span><span style="font-family:Times New Roman;font-size:12.0pt;font-style:italic">Chlamydomonas reinhardti</span></span><span><span style="font-family:Times New Roman;font-size:12.0pt"> was significantly different from the </span></span><span><span style="font-family:Times New Roman;font-size:12.0pt;font-style:italic">E. Coli</span></span><span><span style="font-family:Times New Roman;font-size:12.0pt">. At last, we decided to synthesis the codon-optimized DNA sequence to replace it.<span></span></span></span></p>
 
<p style="text-align:left;text-indent:21.0pt"><span><span style="font-family:Times New Roman;font-size:12.0pt">We have mentioned in the wiki that we co-expressed </span></span><span><span style="font-family:Times New Roman;font-size:12.0pt;font-style:italic">Cry</span></span><span><span style="font-family:Times New Roman;font-size:12.0pt"> and </span></span><span><span style="font-family:Times New Roman;font-size:12.0pt;font-style:italic">Cyt</span></span><span><span style="font-family:Times New Roman;font-size:12.0pt"> by 2A-peptide system. So we also submit the codon-optimized </span></span><span><span style="font-family:Times New Roman;font-size:12.0pt;font-style:italic">Cry11Aa </span></span><span><span style="font-family:Times New Roman;font-size:12.0pt">with 2A-peptide sequence part. Users can link the other genes by infusion technology.<span></span></span></span></p>
 
<p style="text-align:left;text-indent:21.0pt"><span><span style="font-family:Times New Roman;font-size:12.0pt">Therefore, we submitted the part which contained the </span></span><span><span style="font-family:Times New Roman;font-size:12.0pt;font-style:italic">Chlamydomonas reinhardti</span></span><span><span style="font-family:Times New Roman;font-size:12.0pt"> codon-optimized DNA sequence as a part of our improvement project. Meanwhile, we submit the codon-optimized </span></span><span><span style="font-family:Times New Roman;font-size:12.0pt;font-style:italic">Cry11Aa </span></span><span><span style="font-family:Times New Roman;font-size:12.0pt">with 2A-peptide sequence part.<span></span></span></span></p>
 
<p style="text-align:left"><span></span><span style="font-family:Times New Roman;font-size:12.0pt">&nbsp;</span></p>
 
<p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt">&nbsp;</span></p>
 
<p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt">&nbsp;</span></p>
 
<p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt">&nbsp;</span></p>
 
<p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt">&nbsp;</span></p>
 
<p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt">&nbsp;</span></p>
 
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<p style="text-align:left;text-indent:21.0pt"><br/>
 
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<p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt">&nbsp;</span></p>
 
<p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt">&nbsp;</span></p>
 
<p style="text-align:left"><span style="font-family:Times New Roman;font-size:12.0pt">&nbsp;</span></p>
 
<p style="text-align:left"><a name="_GoBack"></a><span style="font-family:Times New Roman;font-size:12.0pt">&nbsp;</span></p>
 
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Latest revision as of 06:42, 8 November 2016


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