Difference between revisions of "Team:OUC-China"

 
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   <meta charset="UTF-8">
 
   <meta charset="UTF-8">
 
   <meta name="viewport" content="width=device-width, initial-scale=1, maximum-scale=1, user-scalable=no">
 
   <meta name="viewport" content="width=device-width, initial-scale=1, maximum-scale=1, user-scalable=no">
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  <meta name="Keywords" content="关键词,关键词">
 
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  <meta name="Description" content="描述">
 
 
   <meta http-equiv="X-UA-Compatible" content="IE=edge">
 
   <meta http-equiv="X-UA-Compatible" content="IE=edge">
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   <link href="https://2016.igem.org/Team:OUC-China/css/bootstrap.min.css" rel="stylesheet">
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   <link rel="stylesheet" href="https://2016.igem.org/Team:OUC-China/css/bootstrap-min?action=raw&amp;ctype=text/css">
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   <script src="http://libs.baidu.com/jquery/2.0.0/jquery.min.js"></script>
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  <link rel="stylesheet" href="https://2016.igem.org/Team:OUC-China/css/ouc_wiki?action=raw&amp;ctype=text/css">
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   <script src="http://libs.baidu.com/bootstrap/3.0.3/js/bootstrap.min.js"></script>
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  <link rel="stylesheet" href="https://2016.igem.org/Team:OUC-China/css/index?action=raw&amp;ctype=text/css">
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   <title>index </title>
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   <script type="text/javascript" src="https://2016.igem.org/Team:OUC-China/js/jquery?action=raw&amp;ctype=text/javascript"></script>
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   <script type="text/javascript" src="https://2016.igem.org/Team:OUC-China/js/bootstrap?action=raw&amp;ctype=text/javascript"></script>
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   <title>Home</title>
 
<style type="text/css">
 
<style type="text/css">
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.oucNav{background:url('image/ouc-nav-1.fw.png');border:0;}
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        .banner-top{
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.oucBrand{width:200px;height:50px;background:url('image/ouc-nav-logo-1.fw.png');}
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            margin-left: 12%;
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ul[class=dropdown-menu]{background:url('image/ouc-nav-1.fw.png');}
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        }
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p{text-align: justify;}
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body{padding-top: 50px;}
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        @media (min-width: 992px){
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/*TOP*/
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            .work-content .col-md-4{
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#oucNav .active a{background-color:#63b5d7;}
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                  width:25%;
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#oucNav li{font-size:1em;}
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                  margin-right: 8%;
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#oucNav li a{color:white;}
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            }
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#oucNav li a:hover{background-color:#63b5d7;}
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/*Footer*/
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        }
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.oucBanner{height:240px;background:url('image/ouc-banner.fw.png');margin-top:-20px;}
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#contentSub, #contentcontainer, #footer-box, #sitesub, #catlinks, #search-controls, #p-logo, .h3, .printfooter, .firstHeading, .visualClear {display: none;} /*-- hides default wiki settings --*/
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.oucInformation{background:#EFF2F6;width:100%;margin:0;}
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.oucInformation b{display:block;font-size:18px;padding-top:50px}
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.oucInformation p:last-child{padding-bottom:50px}
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.oucBottom{width:100%;height:60px;background-color:#152640;}
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/* 超小屏幕（手机，小于 768px） */
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/* 没有任何媒体查询相关的代码，因为这在 Bootstrap 中是默认的（还记得 Bootstrap 是移动设备优先的吗？） */
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.oucBanner h3{color:white;padding-top:90px;font-size:32px;}
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/* 小屏幕（平板，大于等于 768px） */
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@media (min-width:768px) {
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.oucBanner h3{color:white;padding-top:93px;font-size:40px;}
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body{padding-top: 100px;}
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}
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/* 中等屏幕（桌面显示器，大于等于 992px） */
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@media (min-width: 992px) {
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.oucBanner h3{color:white;padding-top:93px;font-size:48px;}
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body{padding-top: 50px;}
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}
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#globalWrapper, #content { /*-- changes default wiki settings --*/
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/* 大屏幕（大桌面显示器，大于等于 1200px） */
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width: 100%;  
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@media (min-width: 1200px) { .oucBanner h3{color:white;padding-top:93px;font-size:54px;} }
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height: 100%;
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/*Main*/
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border: 0px;
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.oucAbstract{max-width:90%;margin:0 auto;margin-top:50px;}
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background-color: transparent;
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.oucAbstract h1{font-size:28px;}
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margin: 0px;
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padding: 0px;
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}
  
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.oucAbstract p{font-size:16px;text-align: justify;max-width:100%;margin:0 auto;margin-top:120px;margin-bottom:50px;}
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    .oucBanner{background:url('https://static.igem.org/mediawiki/2016/e/ee/T--OUC-China--banner.fw.png');}
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.oucMovie img{width:100%;}
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.oucNav{background:url('https://static.igem.org/mediawiki/2016/d/d0/T--OUC-China--nav-1.fw.png');}
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ul[class=dropdown-menu]{background:url(https://static.igem.org/mediawiki/2016/d/d0/T--OUC-China--nav-1.fw.png);}
  
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/*Menu*/
 
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.oucMenu{margin:50px auto;max-width:80%;}
 
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.oucMenu img{display:block;margin:0 auto;}
 
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.oucMenu a{text-decoration:none;display:block;}
 
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.oucMenu a p{color:black;font-size:18px;}
 
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.oucMenuBottom{margin:50px 0 50px 0;}
 
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.oucMenuBottom h1{font-size:30px;}
 
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.oucMenuBottom a{text-decoration:none;display:block;color:white;font-size:28px;background-color:#194081;width:180px;height:50px;text-align:center;border-radius:8px;-moz-border-radius:8px;-webkit-border-radius:8px;margin:0 auto;margin-top:20px;padding-top:4px;}
 
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/* 超小屏幕（手机，小于 768px） */
 
  
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/* 小屏幕（平板，大于等于 768px） */
 
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@media (min-width:768px) {
 
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}
 
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/* 中等屏幕（桌面显示器，大于等于 992px） */
 
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@media (min-width: 992px) {
 
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.oucMenuBottom h1{font-size:46px;}
 
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.oucMenuBottom a{text-decoration:none;display:block;color:white;font-size:40px;background-color:#194081;width:222px;height:66px;text-align:center;border-radius:15px;-moz-border-radius:15px;-webkit-border-radius:15px;margin:0 auto;margin-top:20px;padding-top:4px;}
 
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}
 
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/* 大屏幕（大桌面显示器，大于等于 1200px） */
 
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@media (min-width: 1200px) {
 
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}
 
 
</style>
 
</style>
 
</head>  
 
</head>  
 
<body>  
 
<body>  
 
<div class="container">
 
<div class="container">
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<nav class="oucNav navbar navbar-fixed-top" role="navigation">  
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<nav class="oucNav navbar navbar-default navbar-fixed-top" role="navigation">  
 
<div class="container">
 
<div class="container">
 
<div class="navbar-header">  
 
<div class="navbar-header">  
 
  <button type="button" class="navbar-toggle" data-toggle="collapse"
 
  <button type="button" class="navbar-toggle" data-toggle="collapse"
 
  data-target="#example-navbar-collapse">  
 
  data-target="#example-navbar-collapse">  
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  <span class="sr-only">切换导航</span>  
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  <span class="sr-only">Turn</span>  
 
  <span class="icon-bar"></span>  
 
  <span class="icon-bar"></span>  
 
  <span class="icon-bar"></span>  
 
  <span class="icon-bar"></span>  
 
  <span class="icon-bar"></span>  
 
  <span class="icon-bar"></span>  
 
  </button>  
 
  </button>  
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  <a class="navbar-brand oucBrand" href="#"></a>  
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  <a class="navbar-brand oucBrand" href="#"><img src="https://static.igem.org/mediawiki/2016/f/fc/T--OUC-China--nav-logo-1.fw.png" alt="Brand"></a>  
 
</div>  
 
</div>  
 
<div class="collapse navbar-collapse" id="example-navbar-collapse">  
 
<div class="collapse navbar-collapse" id="example-navbar-collapse">  
 
  <ul class="nav navbar-nav" id="oucNav">  
 
  <ul class="nav navbar-nav" id="oucNav">  
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<li class="active"><a href="#">Home</a></li>
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<li class="active"><a href="https://2016.igem.org/Team:OUC-China">Home</a></li>
 
<li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Project<span class="caret"></span></a>
 
<li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Project<span class="caret"></span></a>
 
<ul class="dropdown-menu">
 
<ul class="dropdown-menu">
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<li><a href="#">Overview</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Description">Description</a></li>
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<li><a href="#">Background</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Design">Design</a></li>
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<li><a href="#">Design</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Proof">Proof of concept </a></li>
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<li><a href="#">Protocol</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Results">Results</a></li>
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<li><a href="#">Result</a></li>
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</ul>
 
</ul>
 
</li>
 
</li>
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<li><a href="#">Model</a></li>
 
 
<li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Parts<span class="caret"></span></a>
 
<li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Parts<span class="caret"></span></a>
 
<ul class="dropdown-menu">
 
<ul class="dropdown-menu">
−
<li><a href="#">Basic part</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Parts">Parts</a></li>
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<li><a href="#">Composite parts</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Basic_Part">Basic part</a></li>
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<li><a href="#">Part collection</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Composite_Part">Composite parts</a></li>
 +
<li><a href="https://2016.igem.org/Team:OUC-China/Part_Collection">Part collection</a></li>
 
</ul>
 
</ul>
 
</li>
 
</li>
−
<li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Attribution<span class="caret"></span></a>
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<li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Human Practice<span class="caret"></span></a>
 
<ul class="dropdown-menu">
 
<ul class="dropdown-menu">
−
<li><a href="#">Support</a></li>
+
<li><a href="https://2016.igem.org/Team:OUC-China/Human_Practices">Overview</a></li>
−
<li><a href="#">Collaboration</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Communicating">Communicating & Improving</a></li>
 +
<li><a href="https://2016.igem.org/Team:OUC-China/Investigating">Investigating & Promoting</a></li>
 +
<li><a href="https://2016.igem.org/Team:OUC-China/Potential_application">Potential application</a></li>
 
</ul>
 
</ul>
 
</li>
 
</li>
 
<li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Team<span class="caret"></span></a>
 
<li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Team<span class="caret"></span></a>
 
<ul class="dropdown-menu">
 
<ul class="dropdown-menu">
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<li><a href="#">Members</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Team">Team</a></li>
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<li><a href="#">Instructors</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Collaborations">Collaborations</a></li>
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<li><a href="#">Advisors</a></li>
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</ul>
 
</ul>
 
</li>
 
</li>
−
<li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Notebook<span class="caret"></span></a>
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<li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Modeling<span class="caret"></span></a>
 
<ul class="dropdown-menu">
 
<ul class="dropdown-menu">
−
<li><a href="#">Journal</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Model">Model</a></li>
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<li><a href="#">Safety</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Software">Software</a></li>
 
</ul>
 
</ul>
 
</li>
 
</li>
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<li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Human Practice<span class="caret"></span></a>
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<li><a href="https://2016.igem.org/Team:OUC-China/Attributions">Attributions</a></li>
 +
<li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Safety<span class="caret"></span></a>
 
<ul class="dropdown-menu">
 
<ul class="dropdown-menu">
−
<li><a href="#">Education</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Notebook">Notebook</a></li>
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<li><a href="#">Communication</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Protocol">Protocol</a></li>
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<li><a href="#">Publicity</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Safety">Safety</a></li>
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<li><a href="#">Product Design</a></li>
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−
<li><a href="#">Laws and regulations</a></li>
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</ul>
 
</ul>
 
</li>
 
</li>
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<div id="carousel-example-generic" class="carousel slide" data-ride="carousel">
 
<div id="carousel-example-generic" class="carousel slide" data-ride="carousel">
 
  <!-- Indicators -->
 
  <!-- Indicators -->
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  <ol class="carousel-indicators">
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  <ol class="carousel-indicators" style="left:20%;margin:0 auto;">
 
<li data-target="#carousel-example-generic" data-slide-to="0" class="active"></li>
 
<li data-target="#carousel-example-generic" data-slide-to="0" class="active"></li>
 
<li data-target="#carousel-example-generic" data-slide-to="1"></li>
 
<li data-target="#carousel-example-generic" data-slide-to="1"></li>
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  <div class="carousel-inner" role="listbox">
 
  <div class="carousel-inner" role="listbox">
 
<div class="item active">
 
<div class="item active">
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<img src="image/DSCF2602.JPG">  
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<img src="https://static.igem.org/mediawiki/2016/5/5c/T--OUC-China--index-5.JPG" alt="img-1">  
 
</div>
 
</div>
 
<div class="item">
 
<div class="item">
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<img src="image/DSCF2573.JPG">
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<img src="https://static.igem.org/mediawiki/2016/c/c1/T--OUC-China--index-2.JPG" alt="img-2">
 
</div>
 
</div>
 
<div class="item">
 
<div class="item">
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  <img src="image/DSCF2576.JPG">
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  <img src="https://static.igem.org/mediawiki/2016/2/2a/T--OUC-China--index-1.JPG" alt="img-3">
 
</div>
 
</div>
 
<div class="item">
 
<div class="item">
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  <img src="image/DSCF2579.JPG">
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  <img src="https://static.igem.org/mediawiki/2016/a/a2/T--OUC-China--index-3.JPG" alt="img-4">
 
</div>
 
</div>
 
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<div class="item">
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  <img src=image/DSCF2572.JPG>
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  <img src="https://static.igem.org/mediawiki/2016/5/54/T--OUC-China--index-4.JPG" alt="img-5">
 
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  <!-- Controls -->
 
  <!-- Controls -->
 
  <a class="left carousel-control" href="#carousel-example-generic" role="button" data-slide="prev">
 
  <a class="left carousel-control" href="#carousel-example-generic" role="button" data-slide="prev">
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<span class="glyphicon glyphicon-chevron-left" aria-hidden="true"></span>
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<img src="https://static.igem.org/mediawiki/2016/5/50/T--OUC-China--index-left.png" style="margin-top:100%" alt="Slide Left">
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<span class="sr-only"></span>
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                                <span class="sr-only"></span>
 
  </a>
 
  </a>
 
  <a class="right carousel-control" href="#carousel-example-generic" role="button" data-slide="next">
 
  <a class="right carousel-control" href="#carousel-example-generic" role="button" data-slide="next">
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<span class="glyphicon glyphicon-chevron-right" aria-hidden="true"></span>
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<img src="https://static.igem.org/mediawiki/2016/f/f7/T--OUC-China--index-right.png" style="margin-top:100%" alt="Slide Right">
 
<span class="sr-only"></span>
 
<span class="sr-only"></span>
 
  </a>
 
  </a>
 
</div>
 
</div>
 
<div class="oucAbstract row">
 
<div class="oucAbstract row">
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<div id="" class="col-xs-5">
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<div class="col-md-5">
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<img src="image/ouc-ab-icon1.png" class="img-responsive">
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<img src="https://static.igem.org/mediawiki/2016/0/0c/T--OUC-China--ab-icon1.png" class="img-responsive" alt="icon">
 
</div>
 
</div>
−
<div id="" class="col-xs-2">
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<div class="col-md-2">
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<h1 class="text-center">Abstract</h1>
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<h3 class="text-center">ABSTRACT</h3>
 
</div>
 
</div>
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<div id="" class="col-xs-5">
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<div class="col-md-5">
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<img src="image/ouc-ab-icon2.png" class="img-responsive">
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<img src="https://static.igem.org/mediawiki/2016/d/d4/T--OUC-China--ab-icon2.png" class="img-responsive" alt="icon">
 
</div>
 
</div>
 +
                        <div class="clearfix"></div>
 
<div class="text-center">
 
<div class="text-center">
−
<p class="text-center">As is known, once forming stem-loops, the oligonucleotides will be more stable than the single-stranded ones. And mRNA with stem-loop at its 3’ or 5’ end often get a longer lifetime than the linear one owe to the stem-loop’s resistance to exonuclease. Our team tend to design a series of stem-loops each followed by the same endonuclease site and are transcribed as one polycistron. Once digested by endonuclease and seperate into several independent fragments, cistrons with different ∆G stem-loops will get different stability, thus influence the amount of expressed proteins. In this way, wecan decouple the expression level of upstream and downstream genes of the same operon by simply designing different stem-loops. Futhermore, with quantitative ∆G of stem-loops, we even can achieve the ratio expression of target proteins. It is a creative regulating method and we attempt to provide a series of standard regulation parts for others.</p>
+
<p>Many applications of synthetic biology require the balanced expression of multiple genes. Our team aimed to develop a tightly regulated element to tune the expression of multiple genes within a polycistron. Inspired by the cellulosome of <i>Clostridium  cellulolyticum</i>, we concentrate on stem-loops inserted into the intergentic regions. When transcribed as one polycistron , digested and seperated into several independent fragments, cistrons with 3' end stem-loops varing folding free energy will get different stability. Thus it influences the amount of expressed proteins to realize cistrons concerto. As creative regulating method it is, we have measured and standardized a series of native and designed stem-loops, transforming into a toolkit for a broad use. Our modeling and software is the further exploration for this amazing mean.</p>
 
</div>
 
</div>
 
</div>
 
</div>
−
<div class="oucMovie">
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<div class="oucMovie" style="background:url('https://static.igem.org/mediawiki/2016/9/99/T--OUC-China--movie-1.fw.png');width:100%;height:400px;">
−
<img src="image/ouc-movie-1.fw.png">
+
<video src="https://static.igem.org/mediawiki/2016/d/de/T--OUC-China--Video1.mp4" controls="controls" width="100%" height="400" style="margin:0 auto;">
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your browser does not support the video tag
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</video>
 
</div>
 
</div>
 
<div class="oucMenu row">
 
<div class="oucMenu row">
−
<div class="col-sm-4">
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<div class="col-xs-4">
−
<a href="#"><img src="image/ouc-menu-icon-1.fw.png" class="img-responsive"><p class="text-center">Purpose</p></a>
+
<a href="https://2016.igem.org/Team:OUC-China/Design"><img src="https://static.igem.org/mediawiki/2016/1/1d/T--OUC-China--menu-icon-2.fw.png" class="img-responsive" alt="Design"><p class="text-center" style="margin-top:20px;">Design</p></a>
 
</div>
 
</div>
−
<div class="col-sm-4">
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<div class="col-xs-4">
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<a href="#"><img src="image/ouc-menu-icon-2.fw.png" class="img-responsive"><p class="text-center">Principle</p></a>
+
<a href="https://2016.igem.org/Team:OUC-China/Model"><img src="https://static.igem.org/mediawiki/2016/4/41/T--OUC-China--menu-icon-1.fw.png" class="img-responsive" alt="Safety"><p class="text-center" style="margin-top:20px;">Safety</p></a>
 
</div>
 
</div>
−
<div class="col-sm-4">
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<div class="col-xs-4">
−
<a href="#"><img src="image/ouc-menu-icon-3.fw.png" class="img-responsive"><p class="text-center">Potential</p></a>
+
<a href="https://2016.igem.org/Team:OUC-China/Collaborations"><img src="https://static.igem.org/mediawiki/2016/8/8a/T--OUC-China--menu-icon-4.fw.png" class="img-responsive" alt="Collaborations"><p class="text-center" style="margin-top:20px;">Collaborations</p></a>
 
</div>
 
</div>
 
<div class="clearfix"></div>
 
<div class="clearfix"></div>
−
<div class="col-sm-4">
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<div class="col-xs-4">
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<a href="#"><img src="image/ouc-menu-icon-4.fw.png" class="img-responsive"><p class="text-center">Attrbution</p></a>
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<a href="https://2016.igem.org/Team:OUC-China/Human_Practices"><img src="https://static.igem.org/mediawiki/2016/0/04/T--OUC-China--menu-icon-6.fw.png" class="img-responsive" alt="Human Practices"><p class="text-center" style="margin-top:20px;">Human Practices</p></a>
 
</div>
 
</div>
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<div class="col-sm-4">
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<h1 class="text-center">Let’s take a fantastic journey!</h1>
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<p>1.Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences</p>
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<b>About</b>
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<p>Designed and built by @ Jasmine Chen and @ Zexin Jiao</p>
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Latest revision as of 03:19, 11 November 2016

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ABSTRACT

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Many applications of synthetic biology require the balanced expression of multiple genes. Our team aimed to develop a tightly regulated element to tune the expression of multiple genes within a polycistron. Inspired by the cellulosome of Clostridium cellulolyticum, we concentrate on stem-loops inserted into the intergentic regions. When transcribed as one polycistron , digested and seperated into several independent fragments, cistrons with 3' end stem-loops varing folding free energy will get different stability. Thus it influences the amount of expressed proteins to realize cistrons concerto. As creative regulating method it is, we have measured and standardized a series of native and designed stem-loops, transforming into a toolkit for a broad use. Our modeling and software is the further exploration for this amazing mean.

Design

Design
Safety

Safety
Collaborations

Collaborations
Human Practices

Human Practices
Team

Team
Model

Model

Cistrons Concerto

Thanks:

1.Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences

2.NEW ENGLAND Biolabs

3.GenScript

Contact us:

E-mail: oucigem@163.com

Designed and built by @ Jasmine Chen and @ Zexin Jiao

We are OUC-iGEM logo-one logo-two