Difference between revisions of "Team:OUC-China"

 
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It has been proved that in Clostridium cellulolyticum, there is a kind of post-transcriptional regulation called selective RNA processing and stabilization (SRPS), which describes how stem-loops make a difference to mRNA level in a polycistron.
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As we all know, stem-loops which have lower folding free energy (∆G) than most oligonucleotides. And mRNA with stem-loops on 3’ or 5’ end often more stable than those without stem-loops. In some extent, the lower (∆G) of stem-loop is, the more stable corresponding mRNA is.
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Our team would like to design a series of stem-loops followed by a putative RNase E site and put them in intergenic region of a polycistron. We hope to decouple the expression level of upstream and downstream genes by this design though they are under the control of the same one promoter. And finally we would give a series of pithy regulation parts for others so that they can have another more choice.
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<h2> Welcome to iGEM 2016! </h2>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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<h5>Before you start: </h5>
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<p> Please read the following pages:</p>
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<li>  <a href="https://2016.igem.org/Requirements">Requirements page </a> </li>
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<li> <a href="https://2016.igem.org/Wiki_How-To">Wiki Requirements page</a></li>
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<h5> Styling your wiki </h5>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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<h5> Wiki template information </h5>
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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<p> <a href="https://2016.igem.org/wiki/index.php?title=Team:Example&action=edit"> Click here to edit this page! </a></p>
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<h5>Tips</h5>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<li>Be clear about what you are doing and how you plan to do this.</li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2016.igem.org/Calendar">iGEM 2016 calendar</a> </li>
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<li>Have lots of fun! </li>
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<h5>Inspiration</h5>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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<h5> Uploading pictures and files </h5>
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<p> You can upload your pictures and files to the iGEM 2016 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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When you upload, set the "Destination Filename" to <code>Team:YourOfficialTeamName/NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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<li><a href="https://2016.igem.org/Team:OUC-China/Description">Description</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Design">Design</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Proof">Proof of concept </a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Results">Results</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Basic_Part">Basic part</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Composite_Part">Composite parts</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Part_Collection">Part collection</a></li>
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<li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Human Practice<span class="caret"></span></a>
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<li><a href="https://2016.igem.org/Team:OUC-China/Human_Practices">Overview</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Communicating">Communicating & Improving</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Investigating">Investigating & Promoting</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Potential_application">Potential application</a></li>
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<li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Team<span class="caret"></span></a>
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<li><a href="https://2016.igem.org/Team:OUC-China/Team">Team</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Model">Model</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Software">Software</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Attributions">Attributions</a></li>
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<li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Safety<span class="caret"></span></a>
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<li><a href="https://2016.igem.org/Team:OUC-China/Notebook">Notebook</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Protocol">Protocol</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Safety">Safety</a></li>
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<h3 class="text-center">ABSTRACT</h3>
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<p>Many applications of synthetic biology require the balanced expression of multiple genes. Our team aimed to develop a tightly regulated element to tune the expression of multiple genes within a polycistron. Inspired by the cellulosome of <i>Clostridium  cellulolyticum</i>, we concentrate on stem-loops inserted into the intergentic regions. When transcribed as one polycistron , digested and seperated into several independent fragments, cistrons with 3' end stem-loops varing folding free energy will get different stability. Thus it influences the amount of expressed proteins to realize cistrons concerto. As creative regulating method it is, we have measured and standardized a series of native and designed stem-loops, transforming into a toolkit for a broad use. Our modeling and software is the further exploration for this amazing mean.</p>
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<a href="https://2016.igem.org/Team:OUC-China/Design"><img src="https://static.igem.org/mediawiki/2016/1/1d/T--OUC-China--menu-icon-2.fw.png" class="img-responsive" alt="Design"><p class="text-center" style="margin-top:20px;">Design</p></a>
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<a href="https://2016.igem.org/Team:OUC-China/Model"><img src="https://static.igem.org/mediawiki/2016/4/41/T--OUC-China--menu-icon-1.fw.png" class="img-responsive" alt="Safety"><p class="text-center" style="margin-top:20px;">Safety</p></a>
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<a href="https://2016.igem.org/Team:OUC-China/Collaborations"><img src="https://static.igem.org/mediawiki/2016/8/8a/T--OUC-China--menu-icon-4.fw.png" class="img-responsive" alt="Collaborations"><p class="text-center" style="margin-top:20px;">Collaborations</p></a>
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<a href="https://2016.igem.org/Team:OUC-China/Team"><img src="https://static.igem.org/mediawiki/2016/a/ad/T--OUC-China--menu-icon-5.fw.png" class="img-responsive" alt="Team"><p class="text-center" style="margin-top:20px;">Team</p></a>
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<a href="https://2016.igem.org/Team:OUC-China/Software"><img src="https://static.igem.org/mediawiki/2016/0/07/T--OUC-China--menu-icon-3.fw.png" class="img-responsive" alt="Model"><p class="text-center" style="margin-top:20px;">Model</p></a>
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        <h3 class="text-center">Cistrons Concerto</h3>
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<h3>Thanks:</h3>
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<p>1.Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences</p>
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<p>2.NEW ENGLAND Biolabs</p>
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<p>3.GenScript</p>
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<h3>Contact us:</h3>
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<p><b>E-mail</b>: oucigem@163.com</p>
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<p><b>Designed and built</b> by @ Jasmine Chen and @ Zexin Jiao</p>
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<p>We are OUC-iGEM
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Latest revision as of 03:19, 11 November 2016

Home

icon

ABSTRACT

icon

Many applications of synthetic biology require the balanced expression of multiple genes. Our team aimed to develop a tightly regulated element to tune the expression of multiple genes within a polycistron. Inspired by the cellulosome of Clostridium cellulolyticum, we concentrate on stem-loops inserted into the intergentic regions. When transcribed as one polycistron , digested and seperated into several independent fragments, cistrons with 3' end stem-loops varing folding free energy will get different stability. Thus it influences the amount of expressed proteins to realize cistrons concerto. As creative regulating method it is, we have measured and standardized a series of native and designed stem-loops, transforming into a toolkit for a broad use. Our modeling and software is the further exploration for this amazing mean.

Design

Design
Safety

Safety
Collaborations

Collaborations
Human Practices

Human Practices
Team

Team
Model

Model

Cistrons Concerto

Thanks:

1.Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences

2.NEW ENGLAND Biolabs

3.GenScript

Contact us:

E-mail: oucigem@163.com

Designed and built by @ Jasmine Chen and @ Zexin Jiao

We are OUC-iGEM logo-one logo-two