Difference between revisions of "Team:Manchester"

 
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{{Manchester}}
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{{Manchester/CSS1}}
 
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<html>
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<title>IGEM Mancehster Team 2016</title>
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<style>
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.judging{
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    text-align: center;
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    color: gold;
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    padding-bottom: 100px;
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}
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.medal_title{
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    color: #CD7F32;
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}
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.sliver{
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}
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.gold{
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@media screen and (max-width:700px){
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    .bronze_info li{font-size: 12px;}
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.project1 a:link {
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    color: white;
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    font-size: 20px;
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.project1 a:hover{
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.project1:hover{
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.project1:active{
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    background: gold;
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.project1{
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    border-radius: 10px;
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    display: block;
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    width: 30%;
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    margin: 10px 15px 40px 15px;
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}
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div a{
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    position: relative;
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    z-index:50;
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}
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.interactive img{
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    width:100%;
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    font-size: 20px;
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.project{
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    text-align: center;
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    display: block;
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    width: 17%;
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    margin: 80px 15px 10px 15px;
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.project img{
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.project:hover img{
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    opacity: 1;
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}
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/****
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.project a:link, .project1 a:link {
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    color: white;
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    font-size: 20px;
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    display: block;
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    vertical-align:top;
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    padding: 20px;
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}
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.project a:visited, .project1 a:visited {
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    display: block;
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    vertical-align:top;
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    padding: 20px;
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}
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.project a:hover, .project1 a:hover{
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    color: black;
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}
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.project:hover, .project1:hover{
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    background: gold;
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}
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.project a:active .project1 a:active{
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    background: gold;
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}
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.project:active, .project1:active{
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    background: gold;
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}
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.project1{
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    background: orange;
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    text-align: center;
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    border-radius: 10px;
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    display: block;
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    width: 30%;
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    margin: 10px 15px 40px 15px;
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}
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***/
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.titlegiant{
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    font-size: 35px;
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    color: gold;
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    height: 50px;
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}
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.giantjamboree{
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    border-radius: 20px;
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    border: gold 5px solid;
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    padding: 30px;
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    margin-bottom: 50px;
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    margin-top: 90px;
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}
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.wordgiant{
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    padding-right: 20px;
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}
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@media screen and (max-width:700px){
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.interactive img{width:100%;}
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.font20{display:none}
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.font8{display:block}
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.project{width: 95%;}
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}
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@media screen and (max-width:800px){
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.bronze_info img{width:40px;}
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.trophy1 ul li{font-size:9px;}
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.com23{display:none}
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}
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@media screen and (min-width:800px){
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.mol23{display:none;}
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}
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</style>
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<head>
 +
<title>IGEM Manchester Team 2016</title>
 +
<!--default setting for character set and device view-->
 +
<meta charset="utf-8">
 +
<meta name="viewport" content="width=device-width, initial-scale=1">
 +
</head>
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<body>
 
<body>
−
<!--big picture-->
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<!--big picture (banner in CSS1)-->
−
<div >
+
 
−
<center>
+
<div>
−
<img src="https://static.igem.org/mediawiki/2016/7/7b/Manchester_logo.gif" style="width:400px;height:400px;">
+
 
−
</center>
+
<img class="logo" src="https://static.igem.org/mediawiki/2016/7/7b/Manchester_logo.gif" >
 +
 
 
</div>
 
</div>
 +
 +
 +
<div class="somebox" ></div>
 +
<div class="boxx">
  
 
<!--intro text-->
 
<!--intro text-->
 
<div class="column full_size" >
 
<div class="column full_size" >
−
<h2 > Welcome to <strong style="color:green">iGEM 2016</strong>! </strong></h2>
 
−
<p>Welcome to the University of Manchester’s iGEM 2016 Wiki page! We are a team of 11 undergraduate students all studying at the University of Manchester based in the Manchester Institute of Biotechnology under the supervision of Professor Rainer Breitling and Professor Eriko Takano.</p>
 
−
<p>
 
−
<h4>Science Behind AlcoPatch</h4>
 
−
<p>Controlling alcohol consumption can be difficult, especially amongst students in the UK. An alcohol patch that generates a visible colour change according to blood alcohol concentration could be used to monitor the level of intoxication in a person. Having an indicator to show how intoxicated a person is could help reduce undesired consequences of being too drunk </p>
 
−
 
−
<h4>Our Project Plan </h4>
 
−
<p>It is known that the ethanol concentration in sweat is linearly related to the blood alcohol concentration. Based on this, we aim to develop an ethanol biosensor by using synthetic biology with two separate methods to measure intoxication levels. The first mechanism involves the usage of alcohol oxidase (AOX) in a cell-free system to oxidise ethanol to acetaldehyde that produces hydrogen peroxide ($H_2O_2$) as a by-product. $H_2O_2$ is used as an oxidising agent by horseradish peroxidase (HRP) to convert ABTS to produce luminescence. The second mechanism involves activation of ethanol sensitive alcR in engineered Escherichia Coli (E.Coli). The activated transcription factor then activates the promoter alcA leading to the expression of chromoprotein.
 
−
</p>
 
 
<br />
 
<br />
−
<p>We will be updating the Wiki with much more information as we progress through the project so check back often!</p>
+
<center><h1 style="color:orange"> Welcome to <strong style="color:purple">Manchester</strong> <strong style="color:green">iGEM 2016</strong>! </h1></center>
−
<p>
+
<div class="column half_size">
−
Follow our activity on:
+
<img style="margin:auto;" src="https://static.igem.org/mediawiki/2016/d/d6/T--Manchester--groupstand.png" alt="group photo"></img>
−
<br />
+
</div>
−
<br />
+
<div class="column half_size">
−
<h3 class="unique" ><a  href="https://www.facebook.com/uomigem2016" target="_blank" > Facebook </a><h3>
+
<img style="margin:auto" src="https://static.igem.org/mediawiki/2016/9/92/T--Manchester--grouplab.png" alt="group photo"></img>
 +
</div>
  
 
<br />
 
<br />
−
<h3 class="unique1"><a href="https://twitter.com/igemManc" target="_blank">Twitter</a><h3>
+
<p style="font-size:20px;" class="font20">Welcome to the University of Manchester’s iGEM 2016 Wiki page! We are a team of 10
 +
undergraduate students all studying at the The University of Manchester and Manchester Metropolitan University. We are based in the Manchester Institute of Biotechnology under the supervision of Professor Rainer Breitling and Professor Eriko Takano.</p>
 +
 
 +
<p style="font-size:8px;" class="font8">Welcome to the University of Manchester’s iGEM 2016 Wiki page! We are a team of 10
 +
undergraduate students all studying at the The University of Manchester and Manchester Metropolitan University. We are based in the Manchester Institute of Biotechnology under the supervision of Professor Rainer Breitling and Professor Eriko Takano.</p>
 +
 
 +
 
 +
 
 +
 
 +
<!------------------------------Description----------------------------------->
 +
 
 +
<br /><br /><br />
 +
<center>
 +
<h1 style="color:orange">
 +
Project Description
 +
</h1>
 +
</center>
 +
  <br />
 +
 
 +
  <div class="interactive column full_size">
 +
    <center>
 +
    <img src="https://static.igem.org/mediawiki/2016/4/4b/T--Manchester--introimgmain.png" />
 +
    </center>
 +
  </div>
 +
 
 +
  <br />
 +
 
 +
<p style="font-size:20px" class="font20">Self-awareness of alcohol consumption and intoxication levels is surprisingly difficult to achieve. Our alcohol awareness tool, AlcoPatch, provides a <a href="https://2016.igem.org/Team:Manchester/Model/Costing" target="_blank"> cheap </a>, bio-based, sustainable and robust solution to help increasing self-awareness. The AlcoPatch would provide users with a visible colour signal indicating their blood alcohol concentration in real time. Throughout the project, we have worked closely with the <a href="https://2016.igem.org/Team:Manchester/Human_Practices" target="_blank">police, health care providers, charities</a> and potential users to design a product that would meet the real needs of communities around the world. Having a rapid and reliable indicator to show visually when one has had enough could help reduce the undesired consequences of being too drunk. This helps people who do not want to get drunk, but would also have positive knock-on effects to the people around them, from reducing public disturbance to avoiding drink-driving.
 +
</p> 
 +
 
 +
<p style="font-size:8px;" class="font8">Self-awareness of alcohol consumption and intoxication levels can be difficult for anyone to master. Through the exploration of trying to find a niche within our society that our project could be beneficial to, our project’s targeted audience has changed dramatically since the initial idea of the AlcoPatch. We have found that our AlcoPatch would have many applications to the people within our society whose aim is to not actually get drunk! Our patch would provide these people with a visible colour change according to their blood alcohol concentration thus giving them the ability to monitor their personal intoxication levels. Having an indicator to show how intoxicated a person is could help reduce the undesired consequences of being too drunk, the knock-on effects of these to the public services, drink-driving, as well as the prevention of serving alcohol to people who are over the limit and much more. </p> 
 +
 
 +
 
 +
<br /><br />
 +
<h4 style="color:gold">Science Behind AlcoPatch </h4>
 +
<p style="font-size: 20px" class="font20">It is known that the ethanol concentration in sweat is linearly related to the blood alcohol concentration <sup>[3]</sup>. Based on this, we aim to develop an ethanol biosensor by using synthetic biology, based on two separate potential methods to measure intoxication levels. The <a href="https://2016.igem.org/Team:Manchester/Description/mechanism1" target="_blank"> first mechanism</a> involves the use of alcohol oxidase (AOx) in a <a href="https://2016.igem.org/Team:Manchester/Description/mechanism1" target="_blank"> cell-free system</a> to oxidise ethanol to acetaldehyde that produces hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) as a by-product. H<sub>2</sub>O<sub>2</sub> is used as an oxidising agent by horseradish peroxidase (HRP) to convert ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)) to produce a gradient of the colour green. The <a href="https://2016.igem.org/Team:Manchester/Description/mechanism2" target="_blank"> second mechanism</a> involves activation of the ethanol-sensitive transcription factor <a href="http://parts.igem.org/Part:BBa_K2092004"target="_blank">alcR</a> in engineered <i>Escherichia coli (E. coli) </i>. The activated transcription factor then activates the <a href="http://parts.igem.org/Part:BBa_K2092003"target="_blank"> alcA promoter </a> leading to the expression of chromoproteins.
 +
 
 +
</p>
 +
 
 +
<p style="font-size: 8px" class="font8">It is known that the ethanol concentration in sweat is linearly related to the blood alcohol concentration. Based on this, we aim to develop an ethanol biosensor by using synthetic biology with two separate methods to measure intoxication levels. The first mechanism involves the usage of alcohol oxidase (AOx) in a cell-free system to oxidise ethanol to acetaldehyde that produces hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) as a by-product. H<sub>2</sub>O<sub>2</sub> is used as an oxidising agent by horseradish peroxidase (HRP) to convert ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)) to produce a colour change. The second mechanism involves activation of ethanol sensitive alcR in engineered Escherichia Coli (E.Coli). The activated transcription factor then activates the promoter alcA leading to the expression of chromoprotein.
 +
</p>
 +
 
 +
<!--------------------------------------------------judging----------------------------------------------------------------->
 +
</br></br>
 +
 
 +
<h4 style="color:gold">Judging Criteria </h4>
 +
 
 +
 
 +
<div class="trophy1 column width33">
 +
  <center>
 +
  <img class="width80" src="https://static.igem.org/mediawiki/2016/1/13/T--Manchester--Trophy_Bronze.png" alt="Bronze Trophy" />
 +
  </center>
 +
 
 +
  <ul class="bronze_info">
 +
  <img class="tick" src="https://static.igem.org/mediawiki/2016/0/0d/T--Manchester--Tick_Bronze.png" alt="Tick" />
 +
    <li>See you at the Giant Jamboree!</li>
 +
  <img class="tick" src="https://static.igem.org/mediawiki/2016/0/0d/T--Manchester--Tick_Bronze.png" alt="Tick" />
 +
    <li>Created a <a href="https://2016.igem.org/Team:Manchester" target="_blank"> Team Wiki</a>. Welcome, you are currently on it! </li>
 +
  <img class="tick" src="https://static.igem.org/mediawiki/2016/0/0d/T--Manchester--Tick_Bronze.png" alt="Tick" />
 +
    <li>Massive thank you to every individual who has <a href="https://2016.igem.org/Team:Manchester/Attributions" target="_blank"> contributed</a> towards our
 +
project. </li>
 +
  <img class="tick" src="https://static.igem.org/mediawiki/2016/0/0d/T--Manchester--Tick_Bronze.png" alt="Tick" />
 +
    <li>Click to find out more about our <a href="https://2016.igem.org/Safety/Check_In?team_id=2092" target="_blank"> safety check-in</a>, <a href="https://2016.igem.org/Safety/About_Our_Lab?team_id=2092" target="_blank"> lab</a>, <a href="https://2016.igem.org/Safety/About_Our_Project?team_id=2092" target="_blank"> project</a>, <a href="https://2016.igem.org/Safety/Final_Safety_Form?team_id=2092" target="_blank"> final safety</a> and <a href="https://igem.org/2016_Judging_Form?id=2092" target="_blank"> judging form</a>.</li>
 +
  <img class="tick" src="https://static.igem.org/mediawiki/2016/0/0d/T--Manchester--Tick_Bronze.png" alt="Tick" />
 +
    <li>Check out one of our new <a href="https://2016.igem.org/Team:Manchester/Parts" target="_blank"> parts</a>, <a href="http://parts.igem.org/Part:BBa_K2092000" target="_blank"> BBa_K2092000</a>, which has been submitted to the iGEM Registry.</li>
 +
  <img class="tick" src="https://static.igem.org/mediawiki/2016/0/0d/T--Manchester--Tick_Bronze.png" alt="Tick" />
 +
    <li>Hope to see all of you at our poster and project presentation at the Giant Jamboree 2016!</li>
 +
 
 +
  </ul>
  
−
</div>
 
  
−
<!--change display to half column-->
 
−
<!--
 
−
<div class="column half_size" >
 
−
<h5>Before you start: </h5>
 
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<p> Please read the following pages:</p>
 
−
<ul>
 
−
<li>  <a href="https://2016.igem.org/Requirements">Requirements page </a> </li>
 
−
<li> <a href="https://2016.igem.org/Wiki_How-To">Wiki Requirements page</a></li>
 
−
<li> <a href="https://2016.igem.org/Resources/Template_Documentation"> Template Documentation </a></li>
 
−
</ul>
 
 
</div>
 
</div>
  
−
<div class="column half_size" >
+
<!--------------------------------------------------------------------------Sliver---------------------------------------------------------------------------------------------->
−
<div class="highlight">
+
 
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<h5> Styling your wiki </h5>
+
 
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
+
<div class="trophy1 column width33">
−
<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>  
+
  <center>
 +
  <img class="width80" src="https://static.igem.org/mediawiki/2016/3/33/T--Manchester--Trophy_Sliver.png" alt="Bronze Trophy" />
 +
  </center>
 +
 
 +
  <ul class="bronze_info">
 +
  <img class="tick" src="https://static.igem.org/mediawiki/2016/b/b4/T--Manchester--Tick_Sliver.png" alt="Tick" />
 +
    <li>Experimentally validated and showed success on our key BioBricks: <a href="http://parts.igem.org/Part:BBa_K2092003" target="_blank"> BBa_K2092003</a>, <a href="http://parts.igem.org/Part:BBa_K2092004" target="_blank"> BBa_K2092004</a>, <a href="http://parts.igem.org/Part:BBa_K2092008" target="_blank"> BBa_K2092008</a> and <a href="http://parts.igem.org/Part:BBa_K2092009" target="_blank"> BBa_K2092009</a>.</li>
 +
  <img class="tick" src="https://static.igem.org/mediawiki/2016/b/b4/T--Manchester--Tick_Sliver.png" alt="Tick" />
 +
    <li>Without the <a href="https://2016.igem.org/Team:Manchester/Collaborations" target="_blank"> collaboration</a> with other iGEM teams, our project would not have been successful. Thank you!</li>
 +
  <img class="tick" src="https://static.igem.org/mediawiki/2016/b/b4/T--Manchester--Tick_Sliver.png" alt="Tick" />
 +
    <li>Reached out to various <a href="https://2016.igem.org/Team:Manchester/Human_Practices" target="_blank"> stakeholders, charities, public services</a> and many others to make sure our device addresses real-world needs in a responsible and efficient way.</li>
 +
 
 +
  </ul> 
 +
 
 +
 
 
</div>
 
</div>
 +
 +
<!--------------------------------------------------------------------------Gold---------------------------------------------------------------------------------------------->
 +
 +
<div class="trophy1 column width33">
 +
  <center>
 +
  <img class="width80" src="https://static.igem.org/mediawiki/2016/9/9b/T--Manchester--Trophy_Gold.png" alt="Bronze Trophy" />
 +
  </center>
 +
 +
    <ul class="bronze_info">
 +
  <img class="tick" src="https://static.igem.org/mediawiki/2016/2/27/T--Manchester--Tick_Gold.png" alt="Tick" />
 +
    <li>Integrated the investigated issues into the design of your project by modelling the <a href="https://2016.igem.org/Team:Manchester/Model/Costing" target="_blank">cost analysis</a> of the AlcoPatch and finding suitable <a href="https://2016.igem.org/Team:Manchester/HP/Gold" target="_blank">alternatives </a> for risk-posing substances used in this project.</li>
 +
  <img class="tick" src="https://static.igem.org/mediawiki/2016/2/27/T--Manchester--Tick_Gold.png" alt="Tick" />
 +
    <li><a href="https://2016.igem.org/Team:Manchester/Description/mechanism2#improved" target="_blank"> Improved</a> an existing BioBrick by adding two missing restriction sites – XbaI and SpeI making assembly process easier. </li>
 +
  <img class="tick" src="https://static.igem.org/mediawiki/2016/2/27/T--Manchester--Tick_Gold.png" alt="Tick" />
 +
    <li>Characterised previously existing BioBricks <a href="http://parts.igem.org/Part:BBa_K1033930 " target="_blank">BBa_K1033930</a> and <a href="http://parts.igem.org/Part:BBa_K1033925" target="_blank">BBa_K1033925</a>.</li>
 +
  <img class="tick" src="https://static.igem.org/mediawiki/2016/2/27/T--Manchester--Tick_Gold.png" alt="Tick" />
 +
    <li>Demonstrated a functional <a href="https://2016.igem.org/Team:Manchester/Proof" target="_blank"> proof of concept</a> of our project.  </li>
 +
  </ul>
 +
 
</div>
 
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−
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<h5> Wiki template information </h5>
 
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
 
  
−
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+
<a class="projectlink" href="https://2016.igem.org/Team:Manchester/Description" title="Project Overview"><img src="https://static.igem.org/mediawiki/2016/b/b1/T--Manchester--mainpage_project.png" alt="Project Overview" /></a>
−
<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>  
+
<center>
−
<p> <a href="https://2016.igem.org/wiki/index.php?title=Team:Example&action=edit"> </a>Use WikiTools - Edit in the black menu bar to edit this page</p>
+
<h3><a href="https://2016.igem.org/Team:Manchester/Description">Project Overview</a></h3>
 +
</center>
 +
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<div class="column project">
 +
<a class="projectlink" href="https://2016.igem.org/Team:Manchester/Achievement" title="Achievement Overview"><img src="https://static.igem.org/mediawiki/2016/3/37/T--Manchester--mainpage_achievement.png" alt="Achievement Overview" /></a>
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<center>
 +
<h3><a href="https://2016.igem.org/Team:Manchester/Achievement">Achievement Overview</a></h3>
 +
</center>
 
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 +
<div class="column project">
 +
<a class="projectlink" href="https://2016.igem.org/Team:Manchester/Human_Practices" title="Human Practices Overview"><img src="https://static.igem.org/mediawiki/2016/d/d9/T--Manchester--mainpage_hp.png" alt="Human Practices Overview" /></a>
 +
<center>
 +
<h3><a href="https://2016.igem.org/Team:Manchester/Human_Practices">Human Practices Overview</a></h3>
 +
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<a class="projectlink" href="https://2016.igem.org/Team:Manchester/Team" title="Team"><img src="https://static.igem.org/mediawiki/2016/8/83/T--Manchester--mainpage_team.png" alt="Team" /></a>
−
<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<ul>
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<h3><a class="projectlink" href="https://2016.igem.org/Team:Manchester/Team">Team</a></h3>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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</center>
−
<li>Be clear about what you are doing and how you plan to do this.</li>
+
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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−
<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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−
<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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−
<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2016.igem.org/Calendar">iGEM 2016 calendar</a> </li>
+
−
<li>Have lots of fun! </li>
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−
</ul>  
+
 
</div>
 
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<div class="column project">
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<a class="projectlink" href="https://2016.igem.org/Team:Manchester/Model" title="Modelling Overview"><img src="https://static.igem.org/mediawiki/2016/5/5c/T--Manchester--mainpage_modelling.png" alt="Modelling Overview" /></a>
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<center>
 +
<h3><a href="https://2016.igem.org/Team:Manchester/Model">Modelling Overview</a></h3>
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<!-------------------------------------------------Giant Jamboree-------------------------------------------------->
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  <h1 class="titlegiant">2016 iGEM Giant Jamboree Results</h1>
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  <br />
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  <div class="half_size column wordgiant">
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    <p class="com23" style="font-size:20px;">Our team attended the Boston iGEM Jamboree and we won a <a style="color:gold" href="https://2016.igem.org/Judging/Medals" target="_blank">Gold Medal</a> for our outstanding efforts, furthermore, we were presented with the prestigious <a href="https://2016.igem.org/Team:Manchester/Model" target="_blank">Best Model </a> award as well as being nominated for the <a href="https://2016.igem.org/Team:Manchester/Engagement" target="_blank">Best Education & Public Engagement</a>. </p>
 +
 +
    <p class="mol23" style="font-size:9px;">Our team attended the Boston iGEM Jamboree and we won a <a style="color:gold" href="https://2016.igem.org/Judging/Medals" target="_blank">Gold Medal</a> for our outstanding efforts, furthermore, we were presented with the prestigious <a href="https://2016.igem.org/Team:Manchester/Model" target="_blank">Best Model </a> award as well as being nominated for the <a href="https://2016.igem.org/Team:Manchester/Engagement" target="_blank">Best Education & Public Engagement</a>. </p>
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  </div>
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    <div class="column half_size">
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    <img src="https://static.igem.org/mediawiki/2016/a/a4/T--Manchester--Best_Model.png" alt="Best Model Picture" />
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<!--
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<div style="clear:both" class="social">
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<p style="font-size: 20px;" class="font20">We will be updating the Wiki with much more information as we progress through the project so check back often!</p>
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<p style="font-size: 20px;" class="font20">
 +
Follow our activity on:
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</p>
 +
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<p style="font-size: 8px;" class="font8">We will be updating the Wiki with much more information as we progress through the project so check back often!</p>
 +
<p style="font-size: 8px;" class="font8">
 +
Follow our activity on:
 +
</p>
 +
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<br />
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<br />
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<a href="https://www.facebook.com/uomigem2016" target="_blank" style="font-size:25px;float:left;"><h3 class="unique"> Facebook
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<a href="https://www.facebook.com/uomigem2016" target="_blank"><img class="socialmedia" style="width:40px;height:40px;float:left;" src="https://static.igem.org/mediawiki/2016/5/57/T--Manchester--Facebook_logo.png" ></img></a>
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</h3>
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<a href="https://twitter.com/iGEMManchester" target="_blank" style="font-size:25px; float:left"><h3 class="unique1"> Twitter
 +
<a href="https://twitter.com/iGEMManchester" target="_blank"><img class="socialmedia" style="width:40px;height:40px;float:left;" src="https://static.igem.org/mediawiki/2016/e/e1/T--Manchester--Twitter_logo.png"></img></a>
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<h1 class="reference">References</h1>
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  <li>Global status report on alcohol and health. 2014 edition. Geneva: World Health Organization; 2014. Available at: <a href="http://apps.who.int/iris/bitstream/10665/112736/1/9789240692763_eng.pdf" target="_blank"> http://apps.who.int/iris/bitstream/10665/112736/1/9789240692763_eng.pdf </a> [Accessed 14 October 2016]</li>
 +
 +
<li>Alcohol concern, (2016). <i>Alcohol statistics</i>. [online]. Available at: <a href="http://www.alcoholconcern.org.uk/alcohol-statistics " target="_blank"> http://www.alcoholconcern.org.uk/alcohol-statistics</a> [Accessed 14 October 2016]</li>
 +
 +
<li>Buono, M.J. (1999). ‘Sweat ethanol concentrations are highly correlated with co-existing blood values in humans’, <i>Experimental Physiology </i>, 84, 401-404.</li>
  
  
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<h5>Inspiration</h5>
 
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
 
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<ul>
 
−
<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
 
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
 
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
 
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
 
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
 
−
<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
 
 
</ul>
 
</ul>
 
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<h5> Uploading pictures and files </h5>
 
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<p> You can upload your pictures and files to the iGEM 2016 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
 
−
When you upload, set the "Destination Filename" to <br><code>T--YourOfficialTeamName--NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
 
  
  
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UPLOAD FILES
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{{Manchester/CSS/footer}}

Latest revision as of 19:46, 26 November 2016

IGEM Manchester Team 2016

Welcome to Manchester iGEM 2016!

group photo
group photo

Welcome to the University of Manchester’s iGEM 2016 Wiki page! We are a team of 10 undergraduate students all studying at the The University of Manchester and Manchester Metropolitan University. We are based in the Manchester Institute of Biotechnology under the supervision of Professor Rainer Breitling and Professor Eriko Takano.

Welcome to the University of Manchester’s iGEM 2016 Wiki page! We are a team of 10 undergraduate students all studying at the The University of Manchester and Manchester Metropolitan University. We are based in the Manchester Institute of Biotechnology under the supervision of Professor Rainer Breitling and Professor Eriko Takano.




Project Description



Self-awareness of alcohol consumption and intoxication levels is surprisingly difficult to achieve. Our alcohol awareness tool, AlcoPatch, provides a cheap , bio-based, sustainable and robust solution to help increasing self-awareness. The AlcoPatch would provide users with a visible colour signal indicating their blood alcohol concentration in real time. Throughout the project, we have worked closely with the police, health care providers, charities and potential users to design a product that would meet the real needs of communities around the world. Having a rapid and reliable indicator to show visually when one has had enough could help reduce the undesired consequences of being too drunk. This helps people who do not want to get drunk, but would also have positive knock-on effects to the people around them, from reducing public disturbance to avoiding drink-driving.

Self-awareness of alcohol consumption and intoxication levels can be difficult for anyone to master. Through the exploration of trying to find a niche within our society that our project could be beneficial to, our project’s targeted audience has changed dramatically since the initial idea of the AlcoPatch. We have found that our AlcoPatch would have many applications to the people within our society whose aim is to not actually get drunk! Our patch would provide these people with a visible colour change according to their blood alcohol concentration thus giving them the ability to monitor their personal intoxication levels. Having an indicator to show how intoxicated a person is could help reduce the undesired consequences of being too drunk, the knock-on effects of these to the public services, drink-driving, as well as the prevention of serving alcohol to people who are over the limit and much more.



Science Behind AlcoPatch

It is known that the ethanol concentration in sweat is linearly related to the blood alcohol concentration [3]. Based on this, we aim to develop an ethanol biosensor by using synthetic biology, based on two separate potential methods to measure intoxication levels. The first mechanism involves the use of alcohol oxidase (AOx) in a cell-free system to oxidise ethanol to acetaldehyde that produces hydrogen peroxide (H2O2) as a by-product. H2O2 is used as an oxidising agent by horseradish peroxidase (HRP) to convert ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)) to produce a gradient of the colour green. The second mechanism involves activation of the ethanol-sensitive transcription factor alcR in engineered Escherichia coli (E. coli) . The activated transcription factor then activates the alcA promoter leading to the expression of chromoproteins.

It is known that the ethanol concentration in sweat is linearly related to the blood alcohol concentration. Based on this, we aim to develop an ethanol biosensor by using synthetic biology with two separate methods to measure intoxication levels. The first mechanism involves the usage of alcohol oxidase (AOx) in a cell-free system to oxidise ethanol to acetaldehyde that produces hydrogen peroxide (H2O2) as a by-product. H2O2 is used as an oxidising agent by horseradish peroxidase (HRP) to convert ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)) to produce a colour change. The second mechanism involves activation of ethanol sensitive alcR in engineered Escherichia Coli (E.Coli). The activated transcription factor then activates the promoter alcA leading to the expression of chromoprotein.



Judging Criteria

Bronze Trophy
Bronze Trophy
Bronze Trophy
    Tick
  • Integrated the investigated issues into the design of your project by modelling the cost analysis of the AlcoPatch and finding suitable alternatives for risk-posing substances used in this project.
    • Tick
  • Improved an existing BioBrick by adding two missing restriction sites – XbaI and SpeI making assembly process easier.
    • Tick
  • Characterised previously existing BioBricks BBa_K1033930 and BBa_K1033925.
    • Tick
  • Demonstrated a functional proof of concept of our project.
Project Overview

Project Overview

Achievement Overview

Achievement Overview

Human Practices Overview

Human Practices Overview

Team

Team

Modelling Overview

Modelling Overview


2016 iGEM Giant Jamboree Results


Our team attended the Boston iGEM Jamboree and we won a Gold Medal for our outstanding efforts, furthermore, we were presented with the prestigious Best Model award as well as being nominated for the Best Education & Public Engagement.

Our team attended the Boston iGEM Jamboree and we won a Gold Medal for our outstanding efforts, furthermore, we were presented with the prestigious Best Model award as well as being nominated for the Best Education & Public Engagement.

Best Model Picture

References

Top ^

Sponsors

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