Latest revision as of 22:04, 30 November 2016

SCSU iGEM Project work · Benchling

SCSU iGEM Project work

Made with Benchling

Project: SCSU_NHiGEM Shared Project

Authors: Bryan Pasqualucci

Dates: 2016-08-17 to 2016-10-19

Wednesday, 8/17

Thomas made 1 liter of LB +AMP for solid media and 1 liter of LB liquid stock (AMP to be added later) according to protocol listed as "Media Preparation."

Poured LB +AMP plates, will flip plates on 8/18

Thomas and Dr. E made AMP stock

Thursday, 8/18

Bryan flipped LB +AMP plates.

Monday, 8/29

Cloning Prep Calculations

Calculation for equimolar amount of digested DNA in ligation step

1.

Digestion of linear backbone PSB1C3 using protocol Cloning Digest. Produced 4 tubes containing 100ng of digested backbone in 8 uL.

2.

Resuspended gBlocks parts , "tnaA V3", "Feedback V3", "Detector" and sfGFP reporter".

a.

gBlocks are 1000ng,resuspend in 100 uL of dH2O for 10 ng/uL

3.

Set up Restriction Digest for parts

a.

	A	B
1	Ingredient	amount
2	dH20	34uL
3	10x Cutsmart	5uL
4	NotI	1uL
5	100ng DNA	10uL

Restriction Digest for parts

Thursday, 9/1

Transformations

Chassis JM109

	A	B	C	D	E
1	Plate Number		Ratio (V:I)	Ligase presents (+/-)	Results (# of colonies)
2	1	Detector	1:1	+
3	2	Feedback	1:1	+
4	3	sfGFP	1:1	+
5	4	tnaA	1:1	+
6	5	Detector	1:2	+	1
7	6	Feedback	1:2	+
8	7	sfGFP	1:2	+
9	8	tnaA	1:2	+	1
10	9	Detector	1:1	-
11	10	Feedback	1:1	-
12	11	sfGFP	1:1	-
13	12	tnaA	1:1	-
14	13	Positive Control	PSBLC3	-	8

Table1

sfGFP- super folder green florescent protein

tnnA- Tryptophanase gene

RESULTS:

Patched out suspected colonies from plates 5 and 8 to grow at 37 overnight.

Friday, 9/2

Patch on plate 8 grew overnight.

Suspected Colonies on plates 5 and 13 were picked and patched onto fresh LB+chloro plates.

Saturday, 9/3

No colonies grew on plate 5 patch.

Colonies from plate 13 and 8 were picked and inoculated into liquid LB+chloro and let grow overnight in 37 degrees C.

---Further work on this moved to tnaA

Saturday, 9/17

Bryan made Master digestion mixture for the linearized PSB1C3 backbone.

	A	B	C
1		Part	Control tube
2	dH2O	17 uL	7 uL
3	10x Cutsmart Buffer	5 uL	1 uL
4	NotI (20U/uL)	1 uL	0.5 uL
5	DnpI (20U/uL)	1 uL	0.5 uL
6	rSAP	1 uL	--
7	total	25 uL	9 uL

Backbone Master Mix

4 uL of the above "Part" mixture and 4 uL of the 25ng/uL linearized Backbone were mixed producing a DNA concentration of 12.5 ng/uL.

1 uL of the 25 ng/uL Linearized Backbone was added to the "Control tube" producing a DNA concentration of 2.5 ng/uL.

These were incubated at 37°C for 30 minutes, then 80°C for 20 minutes to heat kill, then brought to 4°C until ready to ligate.

Bryan made Digests for each of the parts.

	A	B	C	D	E
1		tnaA	Feedback	Detector	sfGFP
2	dH2O	11.5 uL	11.5 uL	11.5 uL	11.5 uL
3	10x cutsmart buffer	2.5 uL	2.5 uL	2.5 uL	2.5 uL
4	gBlock DNA (10ng/uL)	10 uL	10 uL	10 uL	10 uL
5	NotI-HF (20U/uL)	1 uL	1 uL	1 uL	1 uL
6	total volume	25uL	25uL	25uL	25uL

Parts Digest using Noti-HF

DNA now in concentration of 4ng/uL.

Reactions were run at 37°C for 30 minutes then heat killed for 20 minutes at 80°C, then brought to 4°C until ligation step.

	A	B	C	D	E	F
1		tnaA	Feedback	Detector	sfGFP	control
2	dH2O	0.25 uL	0.25 uL	0.25 uL	0.25 uL	3uL
3	10x T4 Ligase Buffer	1 uL	1 uL	1 uL	1 uL	1.5 uL
4	Backbone mixture (12.5ng/uL)	2 uL	2 uL	2 uL	2 uL	---
5	Backbone mixture for control ( 2.5n/uL)	---	---	---	---	10 uL
6	Parts Digest (4ng/uL)	6.25 uL	6.25 uL	6.25 uL	6.25 uL	---
7	T4 ligase	0.5 uL	0.5 uL	0.5 uL	0.5 uL	0.5 uL
8	Total Volume	10 uL	10 uL	10 uL	10 uL	15 uL

Table2

Ligation Reaction run overnight at 16°C (about 20 hours) then heat killed at 80°C for 20 minutes.

Sunday, 9/18

Mixtures were transformed using Transformation JM109 including the SOC outgrowth step and plated on LB+chloro at 37°C.

Monday, 9/19

BP Checked Plates and found no growth. Check transformation protocol, transform with PSB1C3 miniprep DNA next time. Try different plates, there may have been a problem with the Chloro.

May need to test all the chloro plates to find ones that aren't terrible if that is the problem.

SO could be problems : Transformation, plates, design is horribly messed up.

It's not : The enzymes not working, the ligase.

Tuesday, 9/20

Made Enzyme Master mix with rSAP

	A	B	C
1		Master Mix	Control Mix
2	dH2O	17 uL	7 uL
3	10x Cutsmart Buffer	5uL	1 uL
4	Vector	--	1 uL
5	NotI (20U/uL)	1uL	0.5uL
6	DnPI(20U/uL)	1uL	0.5uL
7	rSAP	1 uL	--
8	total volume	25 uL	10 uL

Table3

From "Master Mix" 4uL was added to 4uL linear PSB1C3 and 4uL was added to 4uL of each part gBlock

Ran at 37°C for 30 minutes, then to 80°C for 20 minutes to heat kill, then to 4°C until next use.

Thursday, 9/22

The following Ligation reaction was set up with the previous day's digests.

	A	B	C	D	E	F	G
1		Detector	tnaA	Feedback	sfGFP	-Insert	Control Mix
2	dH2O	0.5 uL	0.5 uL	0.5 uL	0.5 uL	6.5 uL	3 uL
3	10x T4 ligase buffer	1 uL	1 uL	1 uL	1 uL	1 uL	1.5 uL
4	Vector (12.5ng/uL)	2 uL	2 uL	2 uL	2 uL	2 uL	10 uL
5	gBlock digest (4ng/uL)	6.25 uL	6.25 uL	6.25 uL	6.25 uL	--	--
6	T4 ligase	0.5 uL	0.5 uL	0.5 uL	0.5 uL	0.5 uL	0.5 uL
7	total volume	10 uL	10 uL	10 uL	10 uL	10 uL	15 uL

Table4

Reactions run at 16°C overnight, heat killed at 65°C for 20 minutes.

Friday, 9/23

Transformed previous day's ligations using protocol Transformation JM109 with SOC step.

Also transformed 1uL 10pg/uL of J04450 Plasmid from competent cell test kit.

Saturday, 9/24

Figured out why we were having such problems. The liner backbones that are provided have a messed up NotI site due to the way they were PCR produced. So because we have been trying to clone with NotI because of the stuffer sequence problem problems abound. We should break out BBa_J04450 and digest it ourselves with NotI, gel purify it and then try cloning with that.

Broke out BBa_J04450 from the micro wellplate distribution and Transformed it using Transformation JM109

Sunday, 9/25

Streaked out the transformed cells

Wednesday, 9/28

Picked single colonies into two 5mL LB+Chloro liquid cultures, set shaking at 250rpm at 37°C overnight

Thursday, 9/29

Performed miniprep's on the two cultures using Quigen QuickLyse MiniPrep kit (cat. 27406)

Measured Plasmid concentration using NanoDrop

#1 : 119.3 ng/uL

#2 : 23.33 ng/uL

Set up the following digestion

	A	B	C	D
1		#1 (119.3ng/uL)	#1-2 (119.3 ng/uL)	#2 (23.33ng/uL)
2	dH20	18.5 uL	5.5 uL	5.5 uL
3	10x cutsmart buffer	2.5 uL	2.5 uL	2.5 uL
4	BBa_J04450	2 uL	10 uL	10 uL
5	NotI-HF (20u/uL)	1 uL	1 uL	1 uL
6	rSAP	1 uL	1 uL	1 uL
7	total	25 uL	25 uL	25 uL

Table5

Ran at 37°C for 30 minutes, then to 80°C for 20 minutes to heat kill, then to 4°C until next use.

Ran on Gel at 10V overnight (20 hours)

Friday, 9/30

10-1_iGEM_gel_pure_2016.10.01_10.14.15_Fl-UV.jpg

From left to right (2-log ladder, #1-2 , #1, #2)

Extracted the #1-2 and #2 bands using Quigen Gel Extraction Kit (cat. 28706)

Combined the elutions and quantified the DNA using NanoDrop . 10ng/uL in 58uL solution.

Preformed a ligation reaction using the insert digests from 9-17.

	A	B	C
1		1:3 V to Insert	Vector Only
2	dH2O	3.5 uL	19.5 uL
3	10x T4 ligase buffer	2.5 uL	2.5 uL
4	Vector (10ng/uL)	3.0 uL	3.0 uL
5	gBlock digest (4ng/uL)	15 uL	--
6	T4 ligase	1 uL	1 uL
7	total volume	25 uL	25 uL

Table6

Ligation ran at 16°C for 22 hours, Heat kill at 65°C for 10 minutes.

Saturday, 10/1

Transformed the previous ligation reactions using Transformation JM109

Plated and incubated at 37°C.

Sunday, 10/2

Checked plates, no colonies.

Going to redo the Insert Digestion, maybe we shouldn't have used the old digest?

	A	B
1		Detector
2	dH20	2.5 uL
3	10x cutsmart buffer	1.5 uL
4	Detector gBlock (10ng/uL)	10 uL
5	NotI-HF (20u/uL)	1 uL
6	total	15 uL
7	Final Concentration	6.67 ng/uL

Table7

Ran at 37°C for 30 minutes, then to 80°C for 20 minutes to heat kill, then to 4°C until next use.

Monday, 10/3

Ligation of 10-3 Digest

	A	B	C
1		Feedback	Vector Only
2	dH2O	3.5 uL	18.5 uL
3	10x T4 ligase buffer	2.5 uL	2.5 uL
4	Vector (10ng/uL)	3.0 uL	3.0 uL
5	gBlock digest (4ng/uL)	15 uL	--
6	T4 ligase	1 uL	1 uL
7	total volume	25 uL	25 uL

Table8

Ligation ran at 16°C overnight, Heat kill at 65°C for 10 minutes.

Tuesday, 10/4

Transformed ligation mixes into NEB electrocompetent cells (cat. C2989K) using BioRad Gene Pulser Xcell Electroporation System using "Bacterial 1" setting.

	A	B	C	D
1	Cuvette #	Ligation Mix	Tc	Volts
2	1	0.5 pg/uL control BBa_J04450	4.8 ms	1785 V
3	2	10 pg/uL control BBa_J04450	4.9 ms	1786 V
4	3	Vector Only Ligation	4.7 ms	1785 V
5	4	Detector Ligation	4.4 ms	1783 V
6	5	Cells only	5.0 ms	1785 V
7

Table9

Plated onto LB+Chloro plates and incubated at 37°C

Wednesday, 10/5

Checked plates.

Only Control plates had growth.

Thursday, 10/6

Ran mixes with 2-log ladder on a Gel at 100V for one hour.

10-6_iGEMgel_ligmixs_2016.10.06_15.06.55_Fl-UV.jpg

It looks like almost nothing is there.... So concentration issues?

Going to redo the BBa_J04450 digest so we can get more vector.

See about concentration the inserts?

Tuesday, 10/11

Made two 5mL LB+Chloro cultures of BBa_J04450 from colonies isolated on 9/25.

Wednesday, 10/12

Performed miniprep's on the two cultures using Quigen QuickLyse MiniPrep kit (cat. 27406)

Did two preps per 5mL culture.

Combined the preps into 156.6 ng/uL in 196uL.

Thursday, 10/13

Digested the Plasmid from 10/12.

	A	B	C
1		1 ug Plasmid	2 ug Plasmid
2	dH20	37 uL	29 uL
3	10x cutsmart buffer	5 uL	5 uL
4	BBa_J04450	7 uL	14 uL
5	NotI-HF (20u/uL)	1 uL	2 uL
6	total	50 uL	50 uL

Table11

Ran at 37°C for one hour, then to 80°C for 20 minutes to heat kill, then to 4°C until next use.

Friday, 10/14

Digested inserts then will run on Gel

	A	B	C	D
1		Detector	tnaA	Feedback
2	dH20	24 uL	24 uL	24 uL
3	10x Cutsmart Buffer	5 uL	5 uL	5 uL
4	gBlock	20uL	20uL	20uL
5	NotI-HF	1 uL	1 uL	1 uL
6	total volume	50 uL	50 uL	50 uL

Table10

Ran at 37°C for one hour, then concentrated using vacuum centrifuge until volume was 20uL.

Ran 20uL of each of the Detector and Feedback gBlock inserts on a Gel to purify. Along with The 1ug and 2 ug Vector Digestions.

Ran at 10V overnight.

Saturday, 10/15

10-14 VectorDigest 2016.10.14_15.18.11_Fl-UV.jpg

From left to right (2-log ladder, 1ug Vector digest, 2ug Vector Digest, Detector 2wells, Feedback 2 wells)

Extracted the desired bands using Quigen Gel Extraction Kit (cat. 28706)

NanoDrop Results

PSB1C3#1_10-15-16 Default 10/15/2016 11:53 AM 6.453 ng/ul 0.129 0.101 1.28 0.03 50.00 260 0.126 -0.018 DNA-50

PSB1C3#2_10-15-16 Default 10/15/2016 11:54 AM 9.759 ng/ul 0.195 0.144 1.36 0.04 50.00 260 0.191 0.027 DNA-50

Detector#1_10-15-16 Default 10/15/2016 11:55 AM 7.265 ng/ul 0.145 0.105 1.38 0.02 50.00 260 0.141 0.006 DNA-50

Detector#2_10-15-16 Default 10/15/2016 11:58 AM 6.098 ng/ul 0.122 0.100 1.22 0.03 50.00 260 0.118 -0.027 DNA-50

Feedback#1_10-15-16 Default 10/15/2016 11:59 AM 10.02 ng/ul 0.200 0.143 1.41 0.03 50.00 260 0.196 0.086 DNA-50

Feedback#2_10-15-16 Default 10/15/2016 12:00 PM3.931 ng/ul 0.079 0.071 1.11 0.08 50.00 260 0.076 0.031 DNA-50

Sunday, 10/16

Set up a Ligation Reaction using previous day's purifications

	A	B	C	D	E	F	G
1		Feedback, 10-15 Vector	Feedback, 9/30 Vector	Detector, 10-15 Vector	Detector, 9/30 Vector	10-15 Vector Only	9/30 Vector Only
2	dH2O	9 uL	9 uL	9 uL	9 uL	24 uL	24 uL
3	10x T4 ligase buffer	5 uL	5 uL	5 uL	5 uL	5 uL	5 uL
4	Vector	20 uL	20 uL	20 uL	20 uL	20 uL	20 uL
5	gBlock digest	15 uL	15 uL	15 uL	15 uL	--	--
6	T4 ligase	1 uL	1 uL	1 uL	1 uL	1 uL	1 uL
7	total volume	50uL	50uL	50uL	50uL	50uL	50uL

Table12

Incubated at Room temp (25°C for 10 minutes) and transformed using NEB DH5 alpha cells (cat. C2987I)

Plated onto LB+Chloro at 37°C and said a little prayer.

Monday, 10/17

COLONIESSSSS!!!

Those using the 10-15 Vector made red colonies meaning BBa_J04450 didn't get cut out all the way.

But the Vector Only from 9/30 produced very few colonies (2) and the Feedback and Detector produced 9 and 7 respectively.

Picked the 9 colonies from the Feedback plate and made patches and inoculated each into 5mL liquid LB+Chloro, 250rpm at 37°C.

Tuesday, 10/18

Performed two miniprep's per culture using Quigen QuickLyse MiniPrep kit (cat. 27406)

Quantified on the NanoDrop.

Sample ID User ID Date Time Conc. Units A260 A280 260/280 260/230 Conc. Factor (ng/ul) Cursor Pos. Cursor abs. 340 raw NA Type

1-1 Default 10/18/2016 3:08 PM 534.8 ng/ul 10.696 5.249 2.04 1.47 50.00 260 10.668 0.048 DNA-50

1-2 Default 10/18/2016 3:09 PM 308.3 ng/ul 6.165 2.983 2.07 1.29 50.00 260 6.143 0.032 DNA-50

2-1 Default 10/18/2016 3:10 PM 381.4 ng/ul 7.628 3.719 2.05 1.25 50.00 260 7.607 0.007 DNA-50

2-2 Default 10/18/2016 3:11 PM 224.2 ng/ul 4.484 2.180 2.06 1.23 50.00 260 4.468 -0.007 DNA-50

3-1 Default 10/18/2016 3:12 PM 201.5 ng/ul 4.031 2.020 2.00 1.08 50.00 260 4.014 -0.020 DNA-50

3-2 Default 10/18/2016 3:13 PM 316.3 ng/ul 6.325 3.080 2.05 1.16 50.00 260 6.304 -0.008 DNA-50

4-1 Default 10/18/2016 3:14 PM 75.87 ng/ul 1.517 0.793 1.91 0.81 50.00 260 1.508 -0.050 DNA-50

4-2 Default 10/18/2016 3:15 PM 171.8 ng/ul 3.436 1.726 1.99 1.06 50.00 260 3.422 -0.026 DNA-50

5-1 Default 10/18/2016 3:15 PM 96.04 ng/ul 1.921 0.994 1.93 0.91 50.00 260 1.911 -0.254 DNA-50

5-2 Default 10/18/2016 3:16 PM 87.27 ng/ul 1.745 0.900 1.94 0.93 50.00 260 1.735 -0.011 DNA-50

6-1 Default 10/18/2016 3:17 PM 143.7 ng/ul 2.874 1.449 1.98 1.13 50.00 260 2.860 -0.034 DNA-50

6-2 Default 10/18/2016 3:18 PM 79.38 ng/ul 1.588 0.797 1.99 0.93 50.00 260 1.578 -0.046 DNA-50

7-1 Default 10/18/2016 3:19 PM 75.08 ng/ul 1.502 0.778 1.93 0.80 50.00 260 1.493 -0.032 DNA-50

7-2 Default 10/18/2016 3:20 PM 68.95 ng/ul 1.379 0.720 1.91 0.84 50.00 260 1.372 -0.044 DNA-50

8-1 Default 10/18/2016 3:21 PM 84.03 ng/ul 1.681 0.863 1.95 0.80 50.00 260 1.671 -0.009 DNA-50

8-2 Default 10/18/2016 3:22 PM 52.02 ng/ul 1.040 0.544 1.91 0.78 50.00 260 1.033 -0.025 DNA-50

9-1 Default 10/18/2016 3:22 PM 70.20 ng/ul 1.404 0.732 1.92 0.82 50.00 260 1.396 -0.021 DNA-50

9-2 Default 10/18/2016 3:23 PM 122.6 ng/ul 2.451 1.249 1.96 1.03 50.00 260 2.440 -0.053 DNA-50

dh20 Default 10/18/2016 3:24 PM 0.3232 ng/ul 0.006 0.002 3.20 0.17 50.00 260 0.007 -0.071 DNA-50

Combined each culture's prep (ie 1-1 and 1-2 were combined, etc)

Set up the following Digestion to determine insert presence and direction.

	A	B	C	D	E	F	G
1	Tube #	dH2O (uL)	10x cutsmart Buffer (uL)	DNA approx 1 ug (uL)	EcoRI (uL)	SpeI (uL)	total volume (uL)
2	1	18	2.5	2.5	1	1	25
3	2	17	2.5	3.5	1	1	25
4	3	16.35	2.5	4	1	1	25
5	4	12.5	2.5	8	1	1	25
6	5	9.5	2.5	11	1	1	25
7	6	11.5	2.5	9	1	1	25
8	7	6.5	2.5	14	1	1	25
9	8	5.5	2.5	15	1	1	25
10	9	10	2.5	10.5	1	1	25

Table13

Digested at 37°C for 2 hours, 80°C for 20 minutes, then to 4°C until next use.

Wednesday, 10/19

Run them on a Gel and see Results. Gel shows 2kb Vector band and 1.8kb Insert band, in and correct!

Go Home!

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-<body><div><div class="printable-container"><div><div class="printable-page-container"><div class="noteEditor mediocre" tabindex="-1"><div></div><div class="noteEditor-container"><div class="u-cursor-text noteEditor-catcher u-disable-margin-collapse dnd-drop-container"><div class="noteEditor-contentWrapper"><h1 class="mediocre-titleEditor"><div class="editable selectable is-locked" contenteditable="false" data-allow-multiline="false" data-cid="notetitleeditor" data-index="0">SCSU iGEM Project work</div></h1><div><div><i class="faded">Made with Benchling</i></div><div><b>Project:</b> SCSU_NHiGEM Shared Project</div><div><b>Authors:</b> Bryan Pasqualucci</div><div><b>Dates:</b> 2016-08-17 to 2016-10-19</div></div><div class="editor" data-editor="notes"><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 8/17</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part236" data-index="0" style="margin-left:0;">Thomas made 1 liter of LB +AMP for solid media and 1 liter of LB liquid stock (AMP to be added later) according to protocol listed as "Media Preparation." </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part237" data-index="1" style="margin-left:0;">Poured LB +AMP plates, will flip plates on 8/18</div></div></div><div class="mediocre-item is-text is-important"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part238" data-index="2" style="margin-left:0;">Thomas and Dr. E made AMP stock ---- DETAILS ON THIS NEEDED</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 8/18</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part239" data-index="3" style="margin-left:0;">Bryan flipped LB +AMP plates.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part240" data-index="4" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part241" data-index="5" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 8/29</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part242" data-index="6" style="margin-left:0;">Cloning Prep Calculations</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part243" data-index="7" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part244" data-index="8" style="margin-left:0;">Calculation for equamolar amount of digested DNA in ligation step</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part245" data-index="9" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>1.</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part246" data-index="10" style="margin-left:35px;">Digestion of linear backbone  PSB1C3 using protocol   <span>Cloning Digest</span>. Produced 4 tubes containing 100ng of digested backbone in 8 uL. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>2.</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part247" data-index="11" style="margin-left:35px;">Resuspended gBlocks parts , "tnaA V3", "Feedback V3", "Detector" and sfGFP reporter". </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"><span>a.</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part248" data-index="12" style="margin-left:70px;">gBlocks are 1000ng,resuspend in 100 uL of dH2O for 10 ng/uL</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>3.</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part249" data-index="13" style="margin-left:35px;">Set up Restriction Digest for parts</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"><span>a.</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part250" data-index="14" style="margin-left:70px;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part251-item" data-formattable="true" data-index="15" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="15" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part251-table" data-formattable="true" data-index="15" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:265px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:245px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part251-0-0" data-namespace="table-ns2038"><span>Ingredient</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part251-0-1" data-namespace="table-ns2038"><span>amount</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part251-1-0" data-namespace="table-ns2038"><span>dH20</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part251-1-1" data-namespace="table-ns2038"><span>34uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part251-2-0" data-namespace="table-ns2038"><span>10x Cutsmart</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part251-2-1" data-namespace="table-ns2038"><span>5uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part251-3-0" data-namespace="table-ns2038"><span>NotI</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part251-3-1" data-namespace="table-ns2038"><span>1uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part251-4-0" data-namespace="table-ns2038"><span>100ng DNA</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part251-4-1" data-namespace="table-ns2038"><span>10uL</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Restriction Digest for parts</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part251" data-index="15" style="width:265px;margin-left:0;">
+<body><div><div class="printable-container"><div><div class="printable-page-container"><div class="noteEditor mediocre" tabindex="-1"><div></div><div class="noteEditor-container"><div class="u-cursor-text noteEditor-catcher u-disable-margin-collapse dnd-drop-container"><div class="noteEditor-contentWrapper"><h1 class="mediocre-titleEditor"><div class="editable selectable is-locked" contenteditable="false" data-allow-multiline="false" data-cid="notetitleeditor" data-index="0">SCSU iGEM Project work</div></h1><div><div><i class="faded">Made with Benchling</i></div><div><b>Project:</b> SCSU_NHiGEM Shared Project</div><div><b>Authors:</b> Bryan Pasqualucci</div><div><b>Dates:</b> 2016-08-17 to 2016-10-19</div></div><div class="editor" data-editor="notes"><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 8/17</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part236" data-index="0" style="margin-left:0;">Thomas made 1 liter of LB +AMP for solid media and 1 liter of LB liquid stock (AMP to be added later) according to protocol listed as "Media Preparation." </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part237" data-index="1" style="margin-left:0;">Poured LB +AMP plates, will flip plates on 8/18</div></div></div><div class="mediocre-item is-text is-important"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part238" data-index="2" style="margin-left:0;">Thomas and Dr. E made AMP stock</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 8/18</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part239" data-index="3" style="margin-left:0;">Bryan flipped LB +AMP plates.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part240" data-index="4" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part241" data-index="5" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 8/29</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part242" data-index="6" style="margin-left:0;">Cloning Prep Calculations</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part243" data-index="7" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part244" data-index="8" style="margin-left:0;">Calculation for equimolar amount of digested DNA in ligation step</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part245" data-index="9" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>1.</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part246" data-index="10" style="margin-left:35px;">Digestion of linear backbone  PSB1C3 using protocol   <span>Cloning Digest</span>. Produced 4 tubes containing 100ng of digested backbone in 8 uL. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>2.</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part247" data-index="11" style="margin-left:35px;">Resuspended gBlocks parts , "tnaA V3", "Feedback V3", "Detector" and sfGFP reporter". </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"><span>a.</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part248" data-index="12" style="margin-left:70px;">gBlocks are 1000ng,resuspend in 100 uL of dH2O for 10 ng/uL</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"><span>3.</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part249" data-index="13" style="margin-left:35px;">Set up Restriction Digest for parts</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"><span>a.</span></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part250" data-index="14" style="margin-left:70px;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part251-item" data-formattable="true" data-index="15" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="15" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part251-table" data-formattable="true" data-index="15" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:265px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:245px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part251-0-0" data-namespace="table-ns2038"><span>Ingredient</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part251-0-1" data-namespace="table-ns2038"><span>amount</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part251-1-0" data-namespace="table-ns2038"><span>dH20</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part251-1-1" data-namespace="table-ns2038"><span>34uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part251-2-0" data-namespace="table-ns2038"><span>10x Cutsmart</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part251-2-1" data-namespace="table-ns2038"><span>5uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part251-3-0" data-namespace="table-ns2038"><span>NotI</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part251-3-1" data-namespace="table-ns2038"><span>1uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part251-4-0" data-namespace="table-ns2038"><span>100ng DNA</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part251-4-1" data-namespace="table-ns2038"><span>10uL</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Restriction Digest for parts</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part251" data-index="15" style="width:265px;margin-left:0;">
-</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part252" data-index="16" style="margin-left:0;"><b>Thursday, 9/1</b></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part253" data-index="17" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part254" data-index="18" style="margin-left:0;"><b>Transformations</b></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part255" data-index="19" style="margin-left:0;">Chassi JM109</div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part256-item" data-formattable="true" data-index="20" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="20" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part256-table" data-formattable="true" data-index="20" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:565px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:545px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">D</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">E</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-0-0" data-namespace="table-ns2038"><span>Plate Number</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-0-1" data-namespace="table-ns2038"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-0-2" data-namespace="table-ns2038"><span>Ratio (V:I)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-0-3" data-namespace="table-ns2038"><span>Ligase presents (+/-)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-0-4" data-namespace="table-ns2038"><span>Results (# of colonies)</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-value is-alignRight" data-cid="table-note-part256-1-0" data-namespace="table-ns2038"><span>1</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-1-1" data-namespace="table-ns2038"><span>Detector</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-1-2" data-namespace="table-ns2038"><span>1:1</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-1-3" data-namespace="table-ns2038"><span>+</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-1-4" data-namespace="table-ns2038"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-value is-alignRight" data-cid="table-note-part256-2-0" data-namespace="table-ns2038"><span>2</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-2-1" data-namespace="table-ns2038"><span>Feedback</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-2-2" data-namespace="table-ns2038"><span>1:1</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-2-3" data-namespace="table-ns2038"><span>+</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-2-4" data-namespace="table-ns2038"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-value is-alignRight" data-cid="table-note-part256-3-0" data-namespace="table-ns2038"><span>3</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-3-1" data-namespace="table-ns2038"><span>sfGFP</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-3-2" data-namespace="table-ns2038"><span>1:1</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-3-3" data-namespace="table-ns2038"><span>+</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-3-4" data-namespace="table-ns2038"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-value is-alignRight" data-cid="table-note-part256-4-0" data-namespace="table-ns2038"><span>4</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-4-1" data-namespace="table-ns2038"><span>tnaA</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-4-2" data-namespace="table-ns2038"><span>1:1</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-4-3" data-namespace="table-ns2038"><span>+</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-4-4" data-namespace="table-ns2038"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-value is-alignRight" data-cid="table-note-part256-5-0" data-namespace="table-ns2038"><span>5</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-5-1" data-namespace="table-ns2038"><span>Detector</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-5-2" data-namespace="table-ns2038"><span>1:2</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-5-3" data-namespace="table-ns2038"><span>+</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part256-5-4" data-namespace="table-ns2038"><span>1</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">7</span></div></td><td class="cell is-value is-alignRight" data-cid="table-note-part256-6-0" data-namespace="table-ns2038"><span>6</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-6-1" data-namespace="table-ns2038"><span>Feedback</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-6-2" data-namespace="table-ns2038"><span>1:2</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-6-3" data-namespace="table-ns2038"><span>+</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-6-4" data-namespace="table-ns2038"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">8</span></div></td><td class="cell is-value is-alignRight" data-cid="table-note-part256-7-0" data-namespace="table-ns2038"><span>7</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-7-1" data-namespace="table-ns2038"><span>sfGFP</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-7-2" data-namespace="table-ns2038"><span>1:2</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-7-3" data-namespace="table-ns2038"><span>+</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-7-4" data-namespace="table-ns2038"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">9</span></div></td><td class="cell is-value is-alignRight" data-cid="table-note-part256-8-0" data-namespace="table-ns2038"><span>8</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-8-1" data-namespace="table-ns2038"><span>tnaA</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-8-2" data-namespace="table-ns2038"><span>1:2</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-8-3" data-namespace="table-ns2038"><span>+</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part256-8-4" data-namespace="table-ns2038"><span>1</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">10</span></div></td><td class="cell is-value is-alignRight" data-cid="table-note-part256-9-0" data-namespace="table-ns2038"><span>9</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-9-1" data-namespace="table-ns2038"><span>Detector</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-9-2" data-namespace="table-ns2038"><span>1:1</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-9-3" data-namespace="table-ns2038"><span>-</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-9-4" data-namespace="table-ns2038"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">11</span></div></td><td class="cell is-value is-alignRight" data-cid="table-note-part256-10-0" data-namespace="table-ns2038"><span>10</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-10-1" data-namespace="table-ns2038"><span>Feedback</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-10-2" data-namespace="table-ns2038"><span>1:1</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-10-3" data-namespace="table-ns2038"><span>-</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-10-4" data-namespace="table-ns2038"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">12</span></div></td><td class="cell is-value is-alignRight" data-cid="table-note-part256-11-0" data-namespace="table-ns2038"><span>11</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-11-1" data-namespace="table-ns2038"><span>sfGFP</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-11-2" data-namespace="table-ns2038"><span>1:1</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-11-3" data-namespace="table-ns2038"><span>-</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-11-4" data-namespace="table-ns2038"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">13</span></div></td><td class="cell is-value is-alignRight" data-cid="table-note-part256-12-0" data-namespace="table-ns2038"><span>12</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-12-1" data-namespace="table-ns2038"><span>tnaA</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-12-2" data-namespace="table-ns2038"><span>1:1</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-12-3" data-namespace="table-ns2038"><span>-</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-12-4" data-namespace="table-ns2038"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">14</span></div></td><td class="cell is-value is-alignRight" data-cid="table-note-part256-13-0" data-namespace="table-ns2038"><span>13</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-13-1" data-namespace="table-ns2038"><span>Positive Control</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-13-2" data-namespace="table-ns2038"><span>PSBLC3</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-13-3" data-namespace="table-ns2038"><span>-</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part256-13-4" data-namespace="table-ns2038"><span>8</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table1</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part256" data-index="20" style="width:565px;margin-left:0;">
+</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part252" data-index="16" style="margin-left:0;"><b>Thursday, 9/1</b></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part253" data-index="17" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part254" data-index="18" style="margin-left:0;"><b>Transformations</b></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part255" data-index="19" style="margin-left:0;">Chassis JM109</div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part256-item" data-formattable="true" data-index="20" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="20" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part256-table" data-formattable="true" data-index="20" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:565px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:545px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">D</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">E</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-0-0" data-namespace="table-ns2038"><span>Plate Number</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-0-1" data-namespace="table-ns2038"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-0-2" data-namespace="table-ns2038"><span>Ratio (V:I)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-0-3" data-namespace="table-ns2038"><span>Ligase presents (+/-)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-0-4" data-namespace="table-ns2038"><span>Results (# of colonies)</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-value is-alignRight" data-cid="table-note-part256-1-0" data-namespace="table-ns2038"><span>1</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-1-1" data-namespace="table-ns2038"><span>Detector</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-1-2" data-namespace="table-ns2038"><span>1:1</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-1-3" data-namespace="table-ns2038"><span>+</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-1-4" data-namespace="table-ns2038"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-value is-alignRight" data-cid="table-note-part256-2-0" data-namespace="table-ns2038"><span>2</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-2-1" data-namespace="table-ns2038"><span>Feedback</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-2-2" data-namespace="table-ns2038"><span>1:1</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-2-3" data-namespace="table-ns2038"><span>+</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-2-4" data-namespace="table-ns2038"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-value is-alignRight" data-cid="table-note-part256-3-0" data-namespace="table-ns2038"><span>3</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-3-1" data-namespace="table-ns2038"><span>sfGFP</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-3-2" data-namespace="table-ns2038"><span>1:1</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-3-3" data-namespace="table-ns2038"><span>+</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-3-4" data-namespace="table-ns2038"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-value is-alignRight" data-cid="table-note-part256-4-0" data-namespace="table-ns2038"><span>4</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-4-1" data-namespace="table-ns2038"><span>tnaA</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-4-2" data-namespace="table-ns2038"><span>1:1</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-4-3" data-namespace="table-ns2038"><span>+</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-4-4" data-namespace="table-ns2038"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-value is-alignRight" data-cid="table-note-part256-5-0" data-namespace="table-ns2038"><span>5</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-5-1" data-namespace="table-ns2038"><span>Detector</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-5-2" data-namespace="table-ns2038"><span>1:2</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-5-3" data-namespace="table-ns2038"><span>+</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part256-5-4" data-namespace="table-ns2038"><span>1</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">7</span></div></td><td class="cell is-value is-alignRight" data-cid="table-note-part256-6-0" data-namespace="table-ns2038"><span>6</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-6-1" data-namespace="table-ns2038"><span>Feedback</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-6-2" data-namespace="table-ns2038"><span>1:2</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-6-3" data-namespace="table-ns2038"><span>+</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-6-4" data-namespace="table-ns2038"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">8</span></div></td><td class="cell is-value is-alignRight" data-cid="table-note-part256-7-0" data-namespace="table-ns2038"><span>7</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-7-1" data-namespace="table-ns2038"><span>sfGFP</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-7-2" data-namespace="table-ns2038"><span>1:2</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-7-3" data-namespace="table-ns2038"><span>+</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-7-4" data-namespace="table-ns2038"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">9</span></div></td><td class="cell is-value is-alignRight" data-cid="table-note-part256-8-0" data-namespace="table-ns2038"><span>8</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-8-1" data-namespace="table-ns2038"><span>tnaA</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-8-2" data-namespace="table-ns2038"><span>1:2</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-8-3" data-namespace="table-ns2038"><span>+</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part256-8-4" data-namespace="table-ns2038"><span>1</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">10</span></div></td><td class="cell is-value is-alignRight" data-cid="table-note-part256-9-0" data-namespace="table-ns2038"><span>9</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-9-1" data-namespace="table-ns2038"><span>Detector</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-9-2" data-namespace="table-ns2038"><span>1:1</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-9-3" data-namespace="table-ns2038"><span>-</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-9-4" data-namespace="table-ns2038"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">11</span></div></td><td class="cell is-value is-alignRight" data-cid="table-note-part256-10-0" data-namespace="table-ns2038"><span>10</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-10-1" data-namespace="table-ns2038"><span>Feedback</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-10-2" data-namespace="table-ns2038"><span>1:1</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-10-3" data-namespace="table-ns2038"><span>-</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-10-4" data-namespace="table-ns2038"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">12</span></div></td><td class="cell is-value is-alignRight" data-cid="table-note-part256-11-0" data-namespace="table-ns2038"><span>11</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-11-1" data-namespace="table-ns2038"><span>sfGFP</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-11-2" data-namespace="table-ns2038"><span>1:1</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-11-3" data-namespace="table-ns2038"><span>-</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-11-4" data-namespace="table-ns2038"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">13</span></div></td><td class="cell is-value is-alignRight" data-cid="table-note-part256-12-0" data-namespace="table-ns2038"><span>12</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-12-1" data-namespace="table-ns2038"><span>tnaA</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-12-2" data-namespace="table-ns2038"><span>1:1</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-12-3" data-namespace="table-ns2038"><span>-</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-12-4" data-namespace="table-ns2038"><span></span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">14</span></div></td><td class="cell is-value is-alignRight" data-cid="table-note-part256-13-0" data-namespace="table-ns2038"><span>13</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-13-1" data-namespace="table-ns2038"><span>Positive Control</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-13-2" data-namespace="table-ns2038"><span>PSBLC3</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part256-13-3" data-namespace="table-ns2038"><span>-</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part256-13-4" data-namespace="table-ns2038"><span>8</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table1</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part256" data-index="20" style="width:565px;margin-left:0;">
-</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part257" data-index="21" style="margin-left:0;">sfGFP- super folder green florecencent protien </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part258" data-index="22" style="margin-left:0;">tnnA- Tryptophanase gene</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part259" data-index="23" style="margin-left:0;"><b>RESULTS:</b></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part260" data-index="24" style="margin-left:0;"><span style="color:#344043"><span style="background-color:#FFFFFF">Patched out suspected colonies from plates 5 and 8 to grow at 37 overnight.</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part261" data-index="25" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 9/2</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part262" data-index="26" style="margin-left:0;">Patch on plate 8 grew overnight.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part263" data-index="27" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part264" data-index="28" style="margin-left:0;">Suspected Colonies on plates 5 and 13 were picked and patched onto fresh LB+chloro plates.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part265" data-index="29" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Saturday, 9/3</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part266" data-index="30" style="margin-left:0;">No colonies grew on plate 5 patch.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part267" data-index="31" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part268" data-index="32" style="margin-left:0;">Colonies from plate 13 and 8 were picked and innoculated into liquid LB+chloro and let grow overnight in 37 degrees C.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part269" data-index="33" style="margin-left:35px;">---Further work on this moved to tnaA</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part270" data-index="34" style="margin-left:35px;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part271" data-index="35" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Saturday, 9/17</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part272" data-index="36" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part273" data-index="37" style="margin-left:0;">Bryan made Master digestion mixture for the linerized PSB1C3 backbone.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part274" data-index="38" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part275" data-index="39" style="margin-left:0;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part276-item" data-formattable="true" data-index="40" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="40" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part276-table" data-formattable="true" data-index="40" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:365px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:345px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part276-0-0" data-namespace="table-ns2038"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part276-0-1" data-namespace="table-ns2038"><span>Part</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part276-0-2" data-namespace="table-ns2038"><span>Control tube</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part276-1-0" data-namespace="table-ns2038"><span>dH2O</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part276-1-1" data-namespace="table-ns2038"><span>17 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part276-1-2" data-namespace="table-ns2038"><span>7 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part276-2-0" data-namespace="table-ns2038"><span>10x Cutsmart Buffer </span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part276-2-1" data-namespace="table-ns2038"><span>5 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part276-2-2" data-namespace="table-ns2038"><span>1 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part276-3-0" data-namespace="table-ns2038"><span>NotI (20U/uL)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part276-3-1" data-namespace="table-ns2038"><span>1 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part276-3-2" data-namespace="table-ns2038"><span>0.5 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part276-4-0" data-namespace="table-ns2038"><span>DnpI (20U/uL)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part276-4-1" data-namespace="table-ns2038"><span>1 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part276-4-2" data-namespace="table-ns2038"><span>0.5 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part276-5-0" data-namespace="table-ns2038"><span>rSAP</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part276-5-1" data-namespace="table-ns2038"><span>1 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part276-5-2" data-namespace="table-ns2038"><span>--</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">7</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part276-6-0" data-namespace="table-ns2038"><span>total</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part276-6-1" data-namespace="table-ns2038"><span>25 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part276-6-2" data-namespace="table-ns2038"><span>9 uL</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Backbone Master Mix</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part276" data-index="40" style="width:365px;margin-left:0;">
+</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part257" data-index="21" style="margin-left:0;">sfGFP- super folder green florescent protein </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part258" data-index="22" style="margin-left:0;">tnnA- Tryptophanase gene</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part259" data-index="23" style="margin-left:0;"><b>RESULTS:</b></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part260" data-index="24" style="margin-left:0;"><span style="color:#344043"><span style="background-color:#FFFFFF">Patched out suspected colonies from plates 5 and 8 to grow at 37 overnight.</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part261" data-index="25" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 9/2</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part262" data-index="26" style="margin-left:0;">Patch on plate 8 grew overnight.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part263" data-index="27" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part264" data-index="28" style="margin-left:0;">Suspected Colonies on plates 5 and 13 were picked and patched onto fresh LB+chloro plates.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part265" data-index="29" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Saturday, 9/3</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part266" data-index="30" style="margin-left:0;">No colonies grew on plate 5 patch.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part267" data-index="31" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part268" data-index="32" style="margin-left:0;">Colonies from plate 13 and 8 were picked and inoculated into liquid LB+chloro and let grow overnight in 37 degrees C.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part269" data-index="33" style="margin-left:35px;">---Further work on this moved to tnaA</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part270" data-index="34" style="margin-left:35px;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part271" data-index="35" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Saturday, 9/17</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part272" data-index="36" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part273" data-index="37" style="margin-left:0;">Bryan made Master digestion mixture for the linearized PSB1C3 backbone.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part274" data-index="38" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part275" data-index="39" style="margin-left:0;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part276-item" data-formattable="true" data-index="40" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="40" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part276-table" data-formattable="true" data-index="40" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:365px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:345px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part276-0-0" data-namespace="table-ns2038"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part276-0-1" data-namespace="table-ns2038"><span>Part</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part276-0-2" data-namespace="table-ns2038"><span>Control tube</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part276-1-0" data-namespace="table-ns2038"><span>dH2O</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part276-1-1" data-namespace="table-ns2038"><span>17 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part276-1-2" data-namespace="table-ns2038"><span>7 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part276-2-0" data-namespace="table-ns2038"><span>10x Cutsmart Buffer </span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part276-2-1" data-namespace="table-ns2038"><span>5 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part276-2-2" data-namespace="table-ns2038"><span>1 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part276-3-0" data-namespace="table-ns2038"><span>NotI (20U/uL)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part276-3-1" data-namespace="table-ns2038"><span>1 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part276-3-2" data-namespace="table-ns2038"><span>0.5 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part276-4-0" data-namespace="table-ns2038"><span>DnpI (20U/uL)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part276-4-1" data-namespace="table-ns2038"><span>1 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part276-4-2" data-namespace="table-ns2038"><span>0.5 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part276-5-0" data-namespace="table-ns2038"><span>rSAP</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part276-5-1" data-namespace="table-ns2038"><span>1 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part276-5-2" data-namespace="table-ns2038"><span>--</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">7</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part276-6-0" data-namespace="table-ns2038"><span>total</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part276-6-1" data-namespace="table-ns2038"><span>25 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part276-6-2" data-namespace="table-ns2038"><span>9 uL</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Backbone Master Mix</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part276" data-index="40" style="width:365px;margin-left:0;">
-</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part277" data-index="41" style="margin-left:0;">4 uL of the above "Part" mixture and 4 uL of the 25ng/uL Linerized Backbone were mixed producing a DNA concentration of 12.5 ng/uL.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part278" data-index="42" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part279" data-index="43" style="margin-left:0;">1 uL of the 25 ng/uL Linerized Backbone was added to the "Control tube" producing a DNA conacentration of 2.5 ng/uL.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part280" data-index="44" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part281" data-index="45" style="margin-left:0;">These were incubated at 37°C for 30 minutes, then 80<span style="color:#344043"><span style="background-color:#FFFFFF">°C for 20 minutes to heat kill, then brought to 4</span></span><span style="color:#344043"><span style="background-color:#FFFFFF">°C until ready to ligate.</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part282" data-index="46" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part283" data-index="47" style="margin-left:0;">Bryan made Digests for each of the parts.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part284" data-index="48" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part285" data-index="49" style="margin-left:0;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part286-item" data-formattable="true" data-index="50" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="50" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part286-table" data-formattable="true" data-index="50" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:565px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:545px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">D</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">E</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-0-0" data-namespace="table-ns2038"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-0-1" data-namespace="table-ns2038"><span>tnaA </span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-0-2" data-namespace="table-ns2038"><span>Feedback</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-0-3" data-namespace="table-ns2038"><span>Detector</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-0-4" data-namespace="table-ns2038"><span>sfGFP</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-1-0" data-namespace="table-ns2038"><span>dH2O</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-1-1" data-namespace="table-ns2038"><span>11.5 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-1-2" data-namespace="table-ns2038"><span>11.5 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-1-3" data-namespace="table-ns2038"><span>11.5 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-1-4" data-namespace="table-ns2038"><span>11.5 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-2-0" data-namespace="table-ns2038"><span>10x cutsmart buffer</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-2-1" data-namespace="table-ns2038"><span>2.5 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-2-2" data-namespace="table-ns2038"><span>2.5 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-2-3" data-namespace="table-ns2038"><span>2.5 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-2-4" data-namespace="table-ns2038"><span>2.5 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-3-0" data-namespace="table-ns2038"><span>gBlock DNA (10ng/uL)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-3-1" data-namespace="table-ns2038"><span>10 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-3-2" data-namespace="table-ns2038"><span>10 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-3-3" data-namespace="table-ns2038"><span>10 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-3-4" data-namespace="table-ns2038"><span>10 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-4-0" data-namespace="table-ns2038"><span>NotI-HF (20U/uL)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-4-1" data-namespace="table-ns2038"><span>1 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-4-2" data-namespace="table-ns2038"><span>1 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-4-3" data-namespace="table-ns2038"><span>1 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-4-4" data-namespace="table-ns2038"><span>1 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-5-0" data-namespace="table-ns2038"><span>total volume</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-5-1" data-namespace="table-ns2038"><span>25uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-5-2" data-namespace="table-ns2038"><span>25uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-5-3" data-namespace="table-ns2038"><span>25uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-5-4" data-namespace="table-ns2038"><span>25uL</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Parts Digest using Noti-HF</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part286" data-index="50" style="width:565px;margin-left:0;">
+</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part277" data-index="41" style="margin-left:0;">4 uL of the above "Part" mixture and 4 uL of the 25ng/uL linearized Backbone were mixed producing a DNA concentration of 12.5 ng/uL.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part278" data-index="42" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part279" data-index="43" style="margin-left:0;">1 uL of the 25 ng/uL Linearized  Backbone was added to the "Control tube" producing a DNA concentration of 2.5 ng/uL.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part280" data-index="44" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part281" data-index="45" style="margin-left:0;">These were incubated at 37°C for 30 minutes, then 80<span style="color:#344043"><span style="background-color:#FFFFFF">°C for 20 minutes to heat kill, then brought to 4</span></span><span style="color:#344043"><span style="background-color:#FFFFFF">°C until ready to ligate.</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part282" data-index="46" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part283" data-index="47" style="margin-left:0;">Bryan made Digests for each of the parts.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part284" data-index="48" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part285" data-index="49" style="margin-left:0;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part286-item" data-formattable="true" data-index="50" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="50" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part286-table" data-formattable="true" data-index="50" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:565px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:545px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">D</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">E</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-0-0" data-namespace="table-ns2038"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-0-1" data-namespace="table-ns2038"><span>tnaA </span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-0-2" data-namespace="table-ns2038"><span>Feedback</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-0-3" data-namespace="table-ns2038"><span>Detector</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-0-4" data-namespace="table-ns2038"><span>sfGFP</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-1-0" data-namespace="table-ns2038"><span>dH2O</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-1-1" data-namespace="table-ns2038"><span>11.5 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-1-2" data-namespace="table-ns2038"><span>11.5 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-1-3" data-namespace="table-ns2038"><span>11.5 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-1-4" data-namespace="table-ns2038"><span>11.5 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-2-0" data-namespace="table-ns2038"><span>10x cutsmart buffer</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-2-1" data-namespace="table-ns2038"><span>2.5 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-2-2" data-namespace="table-ns2038"><span>2.5 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-2-3" data-namespace="table-ns2038"><span>2.5 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-2-4" data-namespace="table-ns2038"><span>2.5 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-3-0" data-namespace="table-ns2038"><span>gBlock DNA (10ng/uL)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-3-1" data-namespace="table-ns2038"><span>10 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-3-2" data-namespace="table-ns2038"><span>10 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-3-3" data-namespace="table-ns2038"><span>10 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-3-4" data-namespace="table-ns2038"><span>10 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-4-0" data-namespace="table-ns2038"><span>NotI-HF (20U/uL)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-4-1" data-namespace="table-ns2038"><span>1 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-4-2" data-namespace="table-ns2038"><span>1 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-4-3" data-namespace="table-ns2038"><span>1 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-4-4" data-namespace="table-ns2038"><span>1 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-5-0" data-namespace="table-ns2038"><span>total volume</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-5-1" data-namespace="table-ns2038"><span>25uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-5-2" data-namespace="table-ns2038"><span>25uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-5-3" data-namespace="table-ns2038"><span>25uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part286-5-4" data-namespace="table-ns2038"><span>25uL</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Parts Digest using Noti-HF</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part286" data-index="50" style="width:565px;margin-left:0;">
-</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part287" data-index="51" style="margin-left:0;">DNA now in concentation of 4ng/uL. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part288" data-index="52" style="margin-left:0;">Reactions were run at <span style="color:#344043"><span style="background-color:#FFFFFF">37°C for 30 minutes then heat killed for 20 minutes at 80</span></span><span style="color:#344043"><span style="background-color:#FFFFFF">°C, then brought to 4</span></span><span style="color:#344043"><span style="background-color:#FFFFFF">°C until ligation step.</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part289" data-index="53" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part290" data-index="54" style="margin-left:0;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part291-item" data-formattable="true" data-index="55" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="55" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part291-table" data-formattable="true" data-index="55" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:666px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:646px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:101px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">D</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">E</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">F</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-0-0" data-namespace="table-ns2038"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-0-1" data-namespace="table-ns2038"><span>tnaA</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-0-2" data-namespace="table-ns2038"><span>Feedback</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-0-3" data-namespace="table-ns2038"><span>Detector</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-0-4" data-namespace="table-ns2038"><span>sfGFP</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-0-5" data-namespace="table-ns2038"><span>control</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-1-0" data-namespace="table-ns2038"><span>dH2O</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-1-1" data-namespace="table-ns2038"><span>0.25 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-1-2" data-namespace="table-ns2038"><span>0.25 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-1-3" data-namespace="table-ns2038"><span>0.25 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-1-4" data-namespace="table-ns2038"><span>0.25 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-1-5" data-namespace="table-ns2038"><span>3uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-2-0" data-namespace="table-ns2038"><span>10x T4 Ligase Buffer</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-2-1" data-namespace="table-ns2038"><span>1 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-2-2" data-namespace="table-ns2038"><span>1 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-2-3" data-namespace="table-ns2038"><span>1 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-2-4" data-namespace="table-ns2038"><span>1 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-2-5" data-namespace="table-ns2038"><span>1.5 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-3-0" data-namespace="table-ns2038"><span>Backbone mixture (12.5ng/uL)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-3-1" data-namespace="table-ns2038"><span>2 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-3-2" data-namespace="table-ns2038"><span>2 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-3-3" data-namespace="table-ns2038"><span>2 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-3-4" data-namespace="table-ns2038"><span>2 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-3-5" data-namespace="table-ns2038"><span>---</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-4-0" data-namespace="table-ns2038"><span>Backbone mixture for control ( 2.5n/uL)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-4-1" data-namespace="table-ns2038"><span>---</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-4-2" data-namespace="table-ns2038"><span>---</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-4-3" data-namespace="table-ns2038"><span>---</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-4-4" data-namespace="table-ns2038"><span>---</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-4-5" data-namespace="table-ns2038"><span>10 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-5-0" data-namespace="table-ns2038"><span>Parts Digest (4ng/uL)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-5-1" data-namespace="table-ns2038"><span>6.25 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-5-2" data-namespace="table-ns2038"><span>6.25 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-5-3" data-namespace="table-ns2038"><span>6.25 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-5-4" data-namespace="table-ns2038"><span>6.25 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-5-5" data-namespace="table-ns2038"><span>---</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">7</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-6-0" data-namespace="table-ns2038"><span>T4 ligase</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-6-1" data-namespace="table-ns2038"><span>0.5 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-6-2" data-namespace="table-ns2038"><span>0.5 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-6-3" data-namespace="table-ns2038"><span>0.5 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-6-4" data-namespace="table-ns2038"><span>0.5 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-6-5" data-namespace="table-ns2038"><span>0.5 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">8</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-7-0" data-namespace="table-ns2038"><span>Total Volume</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-7-1" data-namespace="table-ns2038"><span>10 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-7-2" data-namespace="table-ns2038"><span>10 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-7-3" data-namespace="table-ns2038"><span>10 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-7-4" data-namespace="table-ns2038"><span>10 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-7-5" data-namespace="table-ns2038"><span>15 uL</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table2</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part291" data-index="55" style="width:666px;margin-left:0;">
+</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part287" data-index="51" style="margin-left:0;">DNA now in concentration of 4ng/uL. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part288" data-index="52" style="margin-left:0;">Reactions were run at <span style="color:#344043"><span style="background-color:#FFFFFF">37°C for 30 minutes then heat killed for 20 minutes at 80</span></span><span style="color:#344043"><span style="background-color:#FFFFFF">°C, then brought to 4</span></span><span style="color:#344043"><span style="background-color:#FFFFFF">°C until ligation step.</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part289" data-index="53" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part290" data-index="54" style="margin-left:0;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part291-item" data-formattable="true" data-index="55" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="55" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part291-table" data-formattable="true" data-index="55" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:666px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:646px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:101px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">D</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">E</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">F</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-0-0" data-namespace="table-ns2038"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-0-1" data-namespace="table-ns2038"><span>tnaA</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-0-2" data-namespace="table-ns2038"><span>Feedback</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-0-3" data-namespace="table-ns2038"><span>Detector</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-0-4" data-namespace="table-ns2038"><span>sfGFP</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-0-5" data-namespace="table-ns2038"><span>control</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-1-0" data-namespace="table-ns2038"><span>dH2O</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-1-1" data-namespace="table-ns2038"><span>0.25 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-1-2" data-namespace="table-ns2038"><span>0.25 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-1-3" data-namespace="table-ns2038"><span>0.25 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-1-4" data-namespace="table-ns2038"><span>0.25 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-1-5" data-namespace="table-ns2038"><span>3uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-2-0" data-namespace="table-ns2038"><span>10x T4 Ligase Buffer</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-2-1" data-namespace="table-ns2038"><span>1 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-2-2" data-namespace="table-ns2038"><span>1 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-2-3" data-namespace="table-ns2038"><span>1 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-2-4" data-namespace="table-ns2038"><span>1 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-2-5" data-namespace="table-ns2038"><span>1.5 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-3-0" data-namespace="table-ns2038"><span>Backbone mixture (12.5ng/uL)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-3-1" data-namespace="table-ns2038"><span>2 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-3-2" data-namespace="table-ns2038"><span>2 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-3-3" data-namespace="table-ns2038"><span>2 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-3-4" data-namespace="table-ns2038"><span>2 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-3-5" data-namespace="table-ns2038"><span>---</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-4-0" data-namespace="table-ns2038"><span>Backbone mixture for control ( 2.5n/uL)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-4-1" data-namespace="table-ns2038"><span>---</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-4-2" data-namespace="table-ns2038"><span>---</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-4-3" data-namespace="table-ns2038"><span>---</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-4-4" data-namespace="table-ns2038"><span>---</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-4-5" data-namespace="table-ns2038"><span>10 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-5-0" data-namespace="table-ns2038"><span>Parts Digest (4ng/uL)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-5-1" data-namespace="table-ns2038"><span>6.25 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-5-2" data-namespace="table-ns2038"><span>6.25 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-5-3" data-namespace="table-ns2038"><span>6.25 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-5-4" data-namespace="table-ns2038"><span>6.25 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-5-5" data-namespace="table-ns2038"><span>---</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">7</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-6-0" data-namespace="table-ns2038"><span>T4 ligase</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-6-1" data-namespace="table-ns2038"><span>0.5 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-6-2" data-namespace="table-ns2038"><span>0.5 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-6-3" data-namespace="table-ns2038"><span>0.5 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-6-4" data-namespace="table-ns2038"><span>0.5 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-6-5" data-namespace="table-ns2038"><span>0.5 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">8</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-7-0" data-namespace="table-ns2038"><span>Total Volume</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-7-1" data-namespace="table-ns2038"><span>10 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-7-2" data-namespace="table-ns2038"><span>10 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-7-3" data-namespace="table-ns2038"><span>10 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-7-4" data-namespace="table-ns2038"><span>10 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part291-7-5" data-namespace="table-ns2038"><span>15 uL</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table2</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part291" data-index="55" style="width:666px;margin-left:0;">
 </div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part292" data-index="56" style="margin-left:0;">Ligation Reaction run overnight at 16<span style="color:#344043"><span style="background-color:#FFFFFF">°C (about 20 hours) then heat killed at 80</span></span><span style="color:#344043"><span style="background-color:#FFFFFF">°C for 20 minutes.</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part293" data-index="57" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part294" data-index="58" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Sunday, 9/18</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part295" data-index="59" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part296" data-index="60" style="margin-left:0;">Mixtures were transformed using <span>Transformation JM109</span> including the SOC outgrowth step and plated on LB+chloro at 37<span style="color:#344043"><span style="background-color:#FFFFFF">°C. </span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part297" data-index="61" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part298" data-index="62" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 9/19</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part299" data-index="63" style="margin-left:0;">BP Checked Plates and found no growth. Check transformation protocol, transform with PSB1C3 miniprep DNA next time. Try different plates, there may have been a problem with the Chloro. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part300" data-index="64" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part301" data-index="65" style="margin-left:0;">May need to test all the chloro plates to find ones that aren't terrible if that is the problem. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part302" data-index="66" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part303" data-index="67" style="margin-left:0;">SO could be problems : Transformation, plates, design is horribly messed up.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part304" data-index="68" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part305" data-index="69" style="margin-left:0;">It's not : The enzymes not working, the ligase.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part306" data-index="70" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part307" data-index="71" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 9/20</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part308" data-index="72" style="margin-left:0;">Made Enzyme Master mix with rSAP</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part309" data-index="73" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part310" data-index="74" style="margin-left:0;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part311-item" data-formattable="true" data-index="75" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="75" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part311-table" data-formattable="true" data-index="75" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:360px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:340px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:95px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part311-0-0" data-namespace="table-ns2038"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part311-0-1" data-namespace="table-ns2038"><span>Master Mix</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part311-0-2" data-namespace="table-ns2038"><span>Control Mix</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part311-1-0" data-namespace="table-ns2038"><span>dH2O</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part311-1-1" data-namespace="table-ns2038"><span>17 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part311-1-2" data-namespace="table-ns2038"><span>7 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part311-2-0" data-namespace="table-ns2038"><span>10x Cutsmart Buffer</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part311-2-1" data-namespace="table-ns2038"><span>5uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part311-2-2" data-namespace="table-ns2038"><span>1 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part311-3-0" data-namespace="table-ns2038"><span>Vector</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part311-3-1" data-namespace="table-ns2038"><span>--</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part311-3-2" data-namespace="table-ns2038"><span>1 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part311-4-0" data-namespace="table-ns2038"><span>NotI (20U/uL)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part311-4-1" data-namespace="table-ns2038"><span>1uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part311-4-2" data-namespace="table-ns2038"><span>0.5uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part311-5-0" data-namespace="table-ns2038"><span>DnPI(20U/uL)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part311-5-1" data-namespace="table-ns2038"><span>1uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part311-5-2" data-namespace="table-ns2038"><span>0.5uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">7</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part311-6-0" data-namespace="table-ns2038"><span>rSAP</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part311-6-1" data-namespace="table-ns2038"><span>1 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part311-6-2" data-namespace="table-ns2038"><span>--</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">8</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part311-7-0" data-namespace="table-ns2038"><span>total volume</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part311-7-1" data-namespace="table-ns2038"><span>25 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part311-7-2" data-namespace="table-ns2038"><span>10 uL</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table3</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part311" data-index="75" style="width:360px;margin-left:0;">
 </div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part312" data-index="76" style="margin-left:0;">From "Master Mix" 4uL was added to 4uL linear PSB1C3 and 4uL was added to 4uL of each part gBlock</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part313" data-index="77" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part314" data-index="78" style="margin-left:0;">Ran at 37°C for 30 minutes, then to 80<span style="color:#344043"><span style="background-color:#FFFFFF">°C for 20 minutes to heat kill, then to 4</span></span><span style="color:#344043"><span style="background-color:#FFFFFF">°C until next use.</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part315" data-index="79" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part316" data-index="80" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 9/22</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part317" data-index="81" style="margin-left:0;">The following Ligation reaction was set up with the previous day's digests.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part318" data-index="82" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part319" data-index="83" style="margin-left:0;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part320-item" data-formattable="true" data-index="84" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="84" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part320-table" data-formattable="true" data-index="84" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:761px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:741px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:95px;"></col><col style="width:101px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">D</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">E</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">F</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">G</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-0-0" data-namespace="table-ns2038"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-0-1" data-namespace="table-ns2038"><span>Detector</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-0-2" data-namespace="table-ns2038"><span>tnaA</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-0-3" data-namespace="table-ns2038"><span>Feedback</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-0-4" data-namespace="table-ns2038"><span>sfGFP</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-0-5" data-namespace="table-ns2038"><span>-Insert</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-0-6" data-namespace="table-ns2038"><span>Control Mix</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-1-0" data-namespace="table-ns2038"><span>dH2O</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-1-1" data-namespace="table-ns2038"><span>0.5 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-1-2" data-namespace="table-ns2038"><span>0.5 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-1-3" data-namespace="table-ns2038"><span>0.5 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-1-4" data-namespace="table-ns2038"><span>0.5 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-1-5" data-namespace="table-ns2038"><span>6.5 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-1-6" data-namespace="table-ns2038"><span>3 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-2-0" data-namespace="table-ns2038"><span>10x T4 ligase buffer</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-2-1" data-namespace="table-ns2038"><span>1 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-2-2" data-namespace="table-ns2038"><span>1 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-2-3" data-namespace="table-ns2038"><span>1 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-2-4" data-namespace="table-ns2038"><span>1 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-2-5" data-namespace="table-ns2038"><span>1 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-2-6" data-namespace="table-ns2038"><span>1.5 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-3-0" data-namespace="table-ns2038"><span>Vector (12.5ng/uL)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-3-1" data-namespace="table-ns2038"><span>2 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-3-2" data-namespace="table-ns2038"><span>2 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-3-3" data-namespace="table-ns2038"><span>2 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-3-4" data-namespace="table-ns2038"><span>2 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-3-5" data-namespace="table-ns2038"><span>2 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-3-6" data-namespace="table-ns2038"><span>10 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-4-0" data-namespace="table-ns2038"><span>gBlock digest (4ng/uL)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-4-1" data-namespace="table-ns2038"><span>6.25 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-4-2" data-namespace="table-ns2038"><span>6.25 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-4-3" data-namespace="table-ns2038"><span>6.25 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-4-4" data-namespace="table-ns2038"><span>6.25 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-4-5" data-namespace="table-ns2038"><span>--</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-4-6" data-namespace="table-ns2038"><span>--</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-5-0" data-namespace="table-ns2038"><span>T4 ligase</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-5-1" data-namespace="table-ns2038"><span>0.5 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-5-2" data-namespace="table-ns2038"><span>0.5 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-5-3" data-namespace="table-ns2038"><span>0.5 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-5-4" data-namespace="table-ns2038"><span>0.5 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-5-5" data-namespace="table-ns2038"><span>0.5 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-5-6" data-namespace="table-ns2038"><span>0.5 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">7</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-6-0" data-namespace="table-ns2038"><span>total volume</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-6-1" data-namespace="table-ns2038"><span>10 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-6-2" data-namespace="table-ns2038"><span>10 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-6-3" data-namespace="table-ns2038"><span>10 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-6-4" data-namespace="table-ns2038"><span>10 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-6-5" data-namespace="table-ns2038"><span>10 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part320-6-6" data-namespace="table-ns2038"><span>15 uL</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table4</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part320" data-index="84" style="width:720px;margin-left:0;">
 </div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part321" data-index="85" style="margin-left:0;">Reactions run at 16<span style="color:#344043"><span style="background-color:#FFFFFF">°C overnight, heat killed at 65</span></span><span style="color:#344043"><span style="background-color:#FFFFFF">°C for 20 minutes. </span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part322" data-index="86" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 9/23</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part323" data-index="87" style="margin-left:0;">Transformed previous day's ligations using protocol <span>Transformation JM109</span> with SOC step. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part324" data-index="88" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part325" data-index="89" style="margin-left:0;">Also transformed 1uL 10pg/uL of J04450 Plasmid from competent cell test kit. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part326" data-index="90" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part327" data-index="91" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Saturday, 9/24</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part328" data-index="92" style="margin-left:0;">Figured out why we were having such problems. The liner backbones that are provided have a messed up NotI site due to the way they were PCR produced. So because we have been trying to clone with NotI because of the stuffer sequence problem problems abound. We should break out BBa_J04450 and digest it ourselves with NotI, gel purify it and then try cloning with that.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part329" data-index="93" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part330" data-index="94" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part331" data-index="95" style="margin-left:0;">Broke out BBa_J04450 from the micro wellplate distribution and Transformed it using <span>Transformation JM109</span> </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part332" data-index="96" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part333" data-index="97" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Sunday, 9/25</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part334" data-index="98" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part335" data-index="99" style="margin-left:0;">Streaked out the transformed cells</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part336" data-index="100" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part337" data-index="101" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 9/28</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part338" data-index="102" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part339" data-index="103" style="margin-left:0;">Picked single colonies into two 5mL LB+Chloro liquid cultures, set shaking at 250rpm at 37<span style="color:#344043"><span style="background-color:#FFFFFF">°C overnight</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part340" data-index="104" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part341" data-index="105" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 9/29</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part342" data-index="106" style="margin-left:0;">Performed miniprep's on the two cultures using Quigen QuickLyse MiniPrep kit (cat. 27406) </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part343" data-index="107" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part344" data-index="108" style="margin-left:0;">Measured Plasmid concentration using NanoDrop </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part345" data-index="109" style="margin-left:35px;">#1 : 119.3 ng/uL</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part346" data-index="110" style="margin-left:35px;">#2 : 23.33 ng/uL</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part347" data-index="111" style="margin-left:35px;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part348" data-index="112" style="margin-left:0;">Set up the following digestion</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part349" data-index="113" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part350" data-index="114" style="margin-left:0;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part351-item" data-formattable="true" data-index="115" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="115" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part351-table" data-formattable="true" data-index="115" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:480px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:460px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:115px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">D</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part351-0-0" data-namespace="table-ns2038"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part351-0-1" data-namespace="table-ns2038"><span>#1 (119.3ng/uL)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part351-0-2" data-namespace="table-ns2038"><span>#1-2 (119.3 ng/uL)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part351-0-3" data-namespace="table-ns2038"><span>#2 (23.33ng/uL)</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part351-1-0" data-namespace="table-ns2038"><span>dH20</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part351-1-1" data-namespace="table-ns2038"><span>18.5 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part351-1-2" data-namespace="table-ns2038"><span>5.5 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part351-1-3" data-namespace="table-ns2038"><span>5.5 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part351-2-0" data-namespace="table-ns2038"><span>10x cutsmart buffer</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part351-2-1" data-namespace="table-ns2038"><span>2.5 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part351-2-2" data-namespace="table-ns2038"><span>2.5 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part351-2-3" data-namespace="table-ns2038"><span>2.5 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part351-3-0" data-namespace="table-ns2038"><span>BBa_J04450</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part351-3-1" data-namespace="table-ns2038"><span>2 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part351-3-2" data-namespace="table-ns2038"><span>10 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part351-3-3" data-namespace="table-ns2038"><span>10 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part351-4-0" data-namespace="table-ns2038"><span>NotI-HF (20u/uL)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part351-4-1" data-namespace="table-ns2038"><span>1 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part351-4-2" data-namespace="table-ns2038"><span>1 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part351-4-3" data-namespace="table-ns2038"><span>1 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part351-5-0" data-namespace="table-ns2038"><span>rSAP</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part351-5-1" data-namespace="table-ns2038"><span>1 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part351-5-2" data-namespace="table-ns2038"><span>1 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part351-5-3" data-namespace="table-ns2038"><span>1 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">7</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part351-6-0" data-namespace="table-ns2038"><span>total</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part351-6-1" data-namespace="table-ns2038"><span>25 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part351-6-2" data-namespace="table-ns2038"><span>25 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part351-6-3" data-namespace="table-ns2038"><span>25 uL</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table5</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part351" data-index="115" style="width:480px;margin-left:0;">
-</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part352" data-index="116" style="margin-left:0;"><span style="color:#344043"><span style="background-color:#FFFFFF">Ran at 37°C for 30 minutes, then to 80</span></span><span style="color:#344043"><span style="background-color:#FFFFFF">°C for 20 minutes to heat kill, then to 4</span></span><span style="color:#344043"><span style="background-color:#FFFFFF">°C until next use.</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part353" data-index="117" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part354" data-index="118" style="margin-left:0;">Ran on Gel at 10V overnight (20 hours)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part355" data-index="119" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part356" data-index="120" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 9/30</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part357" data-index="121" style="margin-left:0;"></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part358-item" data-formattable="true" data-index="122" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:200px;"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">10-1_iGEM_gel_pure_2016.10.01_10.14.15_Fl-UV.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="jy8Okeyi7ZFtaEBEEYmRTUySKm6uYaw8Mw7RNYa0-10-1_iGEM_gel_pure_2016.10.01_10.14.15_Fl-UV.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part358" data-index="122">
+</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part352" data-index="116" style="margin-left:0;"><span style="color:#344043"><span style="background-color:#FFFFFF">Ran at 37°C for 30 minutes, then to 80</span></span><span style="color:#344043"><span style="background-color:#FFFFFF">°C for 20 minutes to heat kill, then to 4</span></span><span style="color:#344043"><span style="background-color:#FFFFFF">°C until next use.</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part353" data-index="117" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part354" data-index="118" style="margin-left:0;">Ran on Gel at 10V overnight (20 hours)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part355" data-index="119" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part356" data-index="120" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 9/30</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part357" data-index="121" style="margin-left:0;"></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part358-item" data-formattable="true" data-index="122" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:200px;"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">10-1_iGEM_gel_pure_2016.10.01_10.14.15_Fl-UV.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="https://static.igem.org/mediawiki/2016/1/1a/SCSU-New_HavenNotebook_Gel10-1.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part358" data-index="122">
 </div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part360" data-index="123" style="margin-left:0;">From left to right (2-log ladder, #1-2 , #1, #2)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part361" data-index="124" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part362" data-index="125" style="margin-left:0;">Extracted the #1-2 and #2 bands using Quigen Gel Extraction Kit (cat. 28706) </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part363" data-index="126" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part364" data-index="127" style="margin-left:0;">Combined the elutions and quantified the DNA using NanoDrop . 10ng/uL in 58uL solution. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part365" data-index="128" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part366" data-index="129" style="margin-left:0;">Preformed a ligation reaction using the insert digests from 9-17.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part367" data-index="130" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part368" data-index="131" style="margin-left:0;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part369-item" data-formattable="true" data-index="132" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="132" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part369-table" data-formattable="true" data-index="132" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:365px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:345px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part369-0-0" data-namespace="table-ns2038"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part369-0-1" data-namespace="table-ns2038"><span>1:3 V to Insert</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part369-0-2" data-namespace="table-ns2038"><span>Vector Only</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part369-1-0" data-namespace="table-ns2038"><span>dH2O</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part369-1-1" data-namespace="table-ns2038"><span>3.5 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part369-1-2" data-namespace="table-ns2038"><span>19.5 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part369-2-0" data-namespace="table-ns2038"><span>10x T4 ligase buffer</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part369-2-1" data-namespace="table-ns2038"><span>2.5 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part369-2-2" data-namespace="table-ns2038"><span>2.5 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part369-3-0" data-namespace="table-ns2038"><span>Vector (10ng/uL)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part369-3-1" data-namespace="table-ns2038"><span>3.0 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part369-3-2" data-namespace="table-ns2038"><span>3.0 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part369-4-0" data-namespace="table-ns2038"><span>gBlock digest (4ng/uL)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part369-4-1" data-namespace="table-ns2038"><span>15 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part369-4-2" data-namespace="table-ns2038"><span>--</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part369-5-0" data-namespace="table-ns2038"><span>T4 ligase</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part369-5-1" data-namespace="table-ns2038"><span>1 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part369-5-2" data-namespace="table-ns2038"><span>1 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">7</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part369-6-0" data-namespace="table-ns2038"><span>total volume</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part369-6-1" data-namespace="table-ns2038"><span>25 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part369-6-2" data-namespace="table-ns2038"><span>25 uL</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table6</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part369" data-index="132" style="width:365px;margin-left:0;">
 </div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part370" data-index="133" style="margin-left:0;">Ligation ran at 16°C for 22 hours, Heat kill at 65<span style="color:#344043"><span style="background-color:#FFFFFF">°C for 10 minutes. </span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part371" data-index="134" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part372" data-index="135" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Saturday, 10/1</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part373" data-index="136" style="margin-left:0;">Transformed the previous ligation reactions using <span>Transformation JM109</span> </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part374" data-index="137" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part375" data-index="138" style="margin-left:0;">Plated and incubated at 37<span style="color:#344043"><span style="background-color:#FFFFFF">°C. </span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part376" data-index="139" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part377" data-index="140" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Sunday, 10/2</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part378" data-index="141" style="margin-left:0;">Checked plates, no colonies. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part379" data-index="142" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part380" data-index="143" style="margin-left:0;"><b>Going to redo the Insert Digestion, maybe we shouldn't have used the old digest?</b></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part381" data-index="144" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part382" data-index="145" style="margin-left:0;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part383-item" data-formattable="true" data-index="146" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="146" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part383-table" data-formattable="true" data-index="146" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:265px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:245px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part383-0-0" data-namespace="table-ns2038"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part383-0-1" data-namespace="table-ns2038"><span>Detector</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part383-1-0" data-namespace="table-ns2038"><span>dH20</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part383-1-1" data-namespace="table-ns2038"><span>2.5 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part383-2-0" data-namespace="table-ns2038"><span>10x cutsmart buffer</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part383-2-1" data-namespace="table-ns2038"><span>1.5 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part383-3-0" data-namespace="table-ns2038"><span>Detector gBlock (10ng/uL)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part383-3-1" data-namespace="table-ns2038"><span>10 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part383-4-0" data-namespace="table-ns2038"><span>NotI-HF (20u/uL)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part383-4-1" data-namespace="table-ns2038"><span>1 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part383-5-0" data-namespace="table-ns2038"><span>total</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part383-5-1" data-namespace="table-ns2038"><span>15 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">7</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part383-6-0" data-namespace="table-ns2038"><span>Final Concentration</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part383-6-1" data-namespace="table-ns2038"><span>6.67 ng/uL</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table7</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part383" data-index="146" style="width:265px;margin-left:0;">
 </div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part384" data-index="147" style="margin-left:0;"><span style="color:#344043"><span style="background-color:#FFFFFF">Ran at 37°C for 30 minutes, then to 80</span></span><span style="color:#344043"><span style="background-color:#FFFFFF">°C for 20 minutes to heat kill, then to 4</span></span><span style="color:#344043"><span style="background-color:#FFFFFF">°C until next use.</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part385" data-index="148" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part386" data-index="149" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 10/3</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part387" data-index="150" style="margin-left:0;">Ligation of 10-3 Digest </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part388" data-index="151" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part389" data-index="152" style="margin-left:0;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part390-item" data-formattable="true" data-index="153" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="153" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part390-table" data-formattable="true" data-index="153" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:361px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:341px;"><colgroup><col style="width:45px;"></col><col style="width:96px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part390-0-0" data-namespace="table-ns2038"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part390-0-1" data-namespace="table-ns2038"><span>Feedback</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part390-0-2" data-namespace="table-ns2038"><span>Vector Only</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part390-1-0" data-namespace="table-ns2038"><span>dH2O</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part390-1-1" data-namespace="table-ns2038"><span>3.5 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part390-1-2" data-namespace="table-ns2038"><span>18.5 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part390-2-0" data-namespace="table-ns2038"><span>10x T4 ligase buffer</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part390-2-1" data-namespace="table-ns2038"><span>2.5 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part390-2-2" data-namespace="table-ns2038"><span>2.5 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part390-3-0" data-namespace="table-ns2038"><span>Vector (10ng/uL)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part390-3-1" data-namespace="table-ns2038"><span>3.0 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part390-3-2" data-namespace="table-ns2038"><span>3.0 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part390-4-0" data-namespace="table-ns2038"><span>gBlock digest (4ng/uL)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part390-4-1" data-namespace="table-ns2038"><span>15 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part390-4-2" data-namespace="table-ns2038"><span>--</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part390-5-0" data-namespace="table-ns2038"><span>T4 ligase</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part390-5-1" data-namespace="table-ns2038"><span>1 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part390-5-2" data-namespace="table-ns2038"><span>1 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">7</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part390-6-0" data-namespace="table-ns2038"><span>total volume</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part390-6-1" data-namespace="table-ns2038"><span>25 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part390-6-2" data-namespace="table-ns2038"><span>25 uL</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table8</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part390" data-index="153" style="width:361px;margin-left:0;">
 </div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part391" data-index="154" style="margin-left:0;"><span style="color:#344043"><span style="background-color:#FFFFFF">Ligation ran at 16°C overnight, Heat kill at 65</span></span><span style="color:#344043"><span style="background-color:#FFFFFF">°C for 10 minutes.</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part392" data-index="155" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part393" data-index="156" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 10/4</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part394" data-index="157" style="margin-left:0;">Transformed ligation mixes into NEB electrocompetent cells (cat. <span style="color:#4C4C4C"><span style="background-color:#FFFFFF">C2989K)</span></span> using BioRad  Gene Pulser Xcell Electroporation System using "Bacterial 1" setting.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part395" data-index="158" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part396" data-index="159" style="margin-left:0;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part397-item" data-formattable="true" data-index="160" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="160" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part397-table" data-formattable="true" data-index="160" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:505px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:485px;"><colgroup><col style="width:45px;"></col><col style="width:98px;"></col><col style="width:142px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">D</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part397-0-0" data-namespace="table-ns2038"><span>Cuvette #</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part397-0-1" data-namespace="table-ns2038"><span>Ligation Mix</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part397-0-2" data-namespace="table-ns2038"><span>Tc </span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part397-0-3" data-namespace="table-ns2038"><span>Volts</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-value is-alignRight" data-cid="table-note-part397-1-0" data-namespace="table-ns2038"><span>1</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part397-1-1" data-namespace="table-ns2038"><span>0.5 pg/uL control BBa_J04450</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part397-1-2" data-namespace="table-ns2038"><span>4.8 ms</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part397-1-3" data-namespace="table-ns2038"><span>1785 V</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-value is-alignRight" data-cid="table-note-part397-2-0" data-namespace="table-ns2038"><span>2</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part397-2-1" data-namespace="table-ns2038"><span>10 pg/uL control BBa_J04450</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part397-2-2" data-namespace="table-ns2038"><span>4.9 ms</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part397-2-3" data-namespace="table-ns2038"><span>1786 V</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-value is-alignRight" data-cid="table-note-part397-3-0" data-namespace="table-ns2038"><span>3</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part397-3-1" data-namespace="table-ns2038"><span>Vector Only Ligation</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part397-3-2" data-namespace="table-ns2038"><span>4.7 ms</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part397-3-3" data-namespace="table-ns2038"><span>1785 V</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-value is-alignRight" data-cid="table-note-part397-4-0" data-namespace="table-ns2038"><span>4</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part397-4-1" data-namespace="table-ns2038"><span>Detector Ligation</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part397-4-2" data-namespace="table-ns2038"><span>4.4 ms</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part397-4-3" data-namespace="table-ns2038"><span>1783 V</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-value is-alignRight" data-cid="table-note-part397-5-0" data-namespace="table-ns2038"><span>5</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part397-5-1" data-namespace="table-ns2038"><span>Cells only</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part397-5-2" data-namespace="table-ns2038"><span>5.0 ms</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part397-5-3" data-namespace="table-ns2038"><span>1785 V</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">7</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part397-6-0" data-namespace="table-ns2038"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part397-6-1" data-namespace="table-ns2038"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part397-6-2" data-namespace="table-ns2038"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part397-6-3" data-namespace="table-ns2038"><span></span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table9</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part397" data-index="160" style="width:505px;margin-left:0;">
-</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part398" data-index="161" style="margin-left:0;">Plated onto LB+Chloro plates and incubated at 37°C</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 10/5</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part399" data-index="162" style="margin-left:0;">Checked plates. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part400" data-index="163" style="margin-left:0;">Only Control plates had growth.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part401" data-index="164" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 10/6</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part402" data-index="165" style="margin-left:0;">Ran mixes with 2-log ladder on a Gel at 100V for one hour. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part403" data-index="166" style="margin-left:0;"></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part404-item" data-formattable="true" data-index="167" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:322px;"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">10-6_iGEMgel_ligmixs_2016.10.06_15.06.55_Fl-UV.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="K55tzyiyr24oWvIrcwwqqTmqWDpl1Hj10PR15rBQ-10-6_iGEMgel_ligmixs_2016.10.06_15.06.55_Fl-UV.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part404" data-index="167">
+</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part398" data-index="161" style="margin-left:0;">Plated onto LB+Chloro plates and incubated at 37°C</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 10/5</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part399" data-index="162" style="margin-left:0;">Checked plates. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part400" data-index="163" style="margin-left:0;">Only Control plates had growth.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part401" data-index="164" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 10/6</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part402" data-index="165" style="margin-left:0;">Ran mixes with 2-log ladder on a Gel at 100V for one hour. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part403" data-index="166" style="margin-left:0;"></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part404-item" data-formattable="true" data-index="167" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:322px;"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">10-6_iGEMgel_ligmixs_2016.10.06_15.06.55_Fl-UV.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="https://static.igem.org/mediawiki/2016/c/cd/SCSU-New_HavenNotebook_Gel10-6.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part404" data-index="167">
 </div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part406" data-index="168" style="margin-left:0;"><b>It looks like almost nothing is there.... So concentration issues?</b></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part407" data-index="169" style="margin-left:0;"><b>Going to redo the BBa_J04450 digest so we can get more vector. </b></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part408" data-index="170" style="margin-left:0;"><b>See about concentration the inserts?</b></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part409" data-index="171" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part410" data-index="172" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 10/11</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part411" data-index="173" style="margin-left:0;">Made two 5mL  LB+Chloro cultures of BBa_J04450 from colonies isolated on 9/25. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part412" data-index="174" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part413" data-index="175" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 10/12</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part414" data-index="176" style="margin-left:0;">Performed miniprep's on the two cultures using Quigen QuickLyse MiniPrep kit (cat. 27406) </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part415" data-index="177" style="margin-left:35px;">Did two preps per 5mL culture.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part416" data-index="178" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part417" data-index="179" style="margin-left:0;">Combined the preps into 156.6 ng/uL in 196uL. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part418" data-index="180" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part419" data-index="181" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 10/13</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part420" data-index="182" style="margin-left:0;">Digested the Plasmid from 10/12.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part421" data-index="183" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part422" data-index="184" style="margin-left:0;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part423-item" data-formattable="true" data-index="185" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="185" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part423-table" data-formattable="true" data-index="185" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:365px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:345px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part423-0-0" data-namespace="table-ns2038"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part423-0-1" data-namespace="table-ns2038"><span>1 ug Plasmid</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part423-0-2" data-namespace="table-ns2038"><span>2 ug Plasmid</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part423-1-0" data-namespace="table-ns2038"><span>dH20</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part423-1-1" data-namespace="table-ns2038"><span>37 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part423-1-2" data-namespace="table-ns2038"><span>29 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part423-2-0" data-namespace="table-ns2038"><span>10x cutsmart buffer</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part423-2-1" data-namespace="table-ns2038"><span>5 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part423-2-2" data-namespace="table-ns2038"><span>5 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part423-3-0" data-namespace="table-ns2038"><span>BBa_J04450</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part423-3-1" data-namespace="table-ns2038"><span>7 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part423-3-2" data-namespace="table-ns2038"><span>14 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part423-4-0" data-namespace="table-ns2038"><span>NotI-HF (20u/uL)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part423-4-1" data-namespace="table-ns2038"><span>1 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part423-4-2" data-namespace="table-ns2038"><span>2 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part423-5-0" data-namespace="table-ns2038"><span>total</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part423-5-1" data-namespace="table-ns2038"><span>50 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part423-5-2" data-namespace="table-ns2038"><span>50 uL</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table11</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part423" data-index="185" style="width:365px;margin-left:0;">
 </div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part424" data-index="186" style="margin-left:0;"><span style="color:#344043"><span style="background-color:#FFFFFF">Ran at 37°C for one hour, then to 80</span></span><span style="color:#344043"><span style="background-color:#FFFFFF">°C for 20 minutes to heat kill, then to 4</span></span><span style="color:#344043"><span style="background-color:#FFFFFF">°C until next use.</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part425" data-index="187" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part426" data-index="188" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 10/14</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part427" data-index="189" style="margin-left:0;">Digested inserts then will run on Gel</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part428" data-index="190" style="margin-left:0;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part429-item" data-formattable="true" data-index="191" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="191" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part429-table" data-formattable="true" data-index="191" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:465px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:445px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">D</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part429-0-0" data-namespace="table-ns2038"><span></span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part429-0-1" data-namespace="table-ns2038"><span>Detector</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part429-0-2" data-namespace="table-ns2038"><span>tnaA</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part429-0-3" data-namespace="table-ns2038"><span>Feedback</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part429-1-0" data-namespace="table-ns2038"><span>dH20</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part429-1-1" data-namespace="table-ns2038"><span>24 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part429-1-2" data-namespace="table-ns2038"><span>24 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part429-1-3" data-namespace="table-ns2038"><span>24 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part429-2-0" data-namespace="table-ns2038"><span>10x Cutsmart Buffer</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part429-2-1" data-namespace="table-ns2038"><span>5 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part429-2-2" data-namespace="table-ns2038"><span>5 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part429-2-3" data-namespace="table-ns2038"><span>5 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part429-3-0" data-namespace="table-ns2038"><span>gBlock </span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part429-3-1" data-namespace="table-ns2038"><span>20uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part429-3-2" data-namespace="table-ns2038"><span>20uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part429-3-3" data-namespace="table-ns2038"><span>20uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part429-4-0" data-namespace="table-ns2038"><span>NotI-HF</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part429-4-1" data-namespace="table-ns2038"><span>1 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part429-4-2" data-namespace="table-ns2038"><span>1 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part429-4-3" data-namespace="table-ns2038"><span>1 uL</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part429-5-0" data-namespace="table-ns2038"><span>total volume</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part429-5-1" data-namespace="table-ns2038"><span>50 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part429-5-2" data-namespace="table-ns2038"><span>50 uL</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part429-5-3" data-namespace="table-ns2038"><span>50 uL</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table10</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part429" data-index="191" style="width:465px;margin-left:0;">
-</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part430" data-index="192" style="margin-left:0;"><span style="color:#344043"><span style="background-color:#FFFFFF">Ran at 37°C for one hour, then concentrated using vacum centrifuge until volume was 20uL.</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part431" data-index="193" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part432" data-index="194" style="margin-left:0;">Ran 20uL of each of the Detector and Feedback gBlock inserts on a Gel to purify.  Along with The 1ug and 2 ug Vector Digestions.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part433" data-index="195" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part434" data-index="196" style="margin-left:0;">Ran at 10V overnight.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part435" data-index="197" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part436" data-index="198" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Saturday, 10/15</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part437" data-index="199" style="margin-left:0;"></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part438-item" data-formattable="true" data-index="200" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:341px;"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">10-14 VectorDigest 2016.10.14_15.18.11_Fl-UV.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="Bh01vL0OXcLLBhTHv2DGCGR8iDujNMXWV804el36-10-14_VectorDigest_2016.10.14_15.18.11_Fl-UV.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part438" data-index="200">
+</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part430" data-index="192" style="margin-left:0;"><span style="color:#344043"><span style="background-color:#FFFFFF">Ran at 37°C for one hour, then concentrated using vacuum centrifuge until volume was 20uL.</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part431" data-index="193" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part432" data-index="194" style="margin-left:0;">Ran 20uL of each of the Detector and Feedback gBlock inserts on a Gel to purify.  Along with The 1ug and 2 ug Vector Digestions.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part433" data-index="195" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part434" data-index="196" style="margin-left:0;">Ran at 10V overnight.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part435" data-index="197" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part436" data-index="198" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Saturday, 10/15</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part437" data-index="199" style="margin-left:0;"></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part438-item" data-formattable="true" data-index="200" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:341px;"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA hidden-for-print" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">10-14 VectorDigest 2016.10.14_15.18.11_Fl-UV.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="https://static.igem.org/mediawiki/2016/1/14/SCSU-New_HavenNotebook_Gel10-14.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part438" data-index="200">
 </div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part440" data-index="201" style="margin-left:0;">From left to right (2-log ladder, 1ug Vector digest, 2ug Vector Digest, Detector 2wells, Feedback 2 wells)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part441" data-index="202" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part442" data-index="203" style="margin-left:0;">Extracted the desired bands using Quigen Gel Extraction Kit (cat. 28706) </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part443" data-index="204" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part444" data-index="205" style="margin-left:0;">NanoDrop Results</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part445" data-index="206" style="margin-left:0;">PSB1C3#1_10-15-16	Default	10/15/2016	11:53 AM	6.453	ng/ul	0.129	0.101	1.28	0.03	50.00	260	0.126	-0.018	DNA-50
 </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part446" data-index="207" style="margin-left:0;">PSB1C3#2_10-15-16	Default	10/15/2016	11:54 AM	9.759	ng/ul	0.195	0.144	1.36	0.04	50.00	260	0.191	0.027	DNA-50
@@ Line 49: / Line 49: @@
 </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part494" data-index="255" style="margin-left:0;">dh20	Default	10/18/2016	3:24 PM	0.3232	ng/ul	0.006	0.002	3.20	0.17	50.00	260	0.007	-0.071	DNA-50
 </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part495" data-index="256" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part496" data-index="257" style="margin-left:0;">Combined  each culture's prep (ie 1-1 and 1-2 were combined, etc)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part497" data-index="258" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part498" data-index="259" style="margin-left:0;">Set up the following Digestion to determine insert presence and direction. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part499" data-index="260" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part500" data-index="261" style="margin-left:0;"></div></div></div><div class="mediocre-item is-captionedTable"><div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part501-item" data-formattable="true" data-index="262" data-subindex="item"></div><div class="mediocre-captionEditable mediocre-captionEditable--table"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell mediocre-captionEditable-component" style="width:100%;"><div class="note-item-wrapper"><div class="mediocre-tableEditable" data-formattable="true" data-index="262" data-subindex="cell" style="margin-left:0;margin-right:0;"><div></div><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part501-table" data-formattable="true" data-index="262" data-subindex="table"></div><div class="mediocre-tableEditable-scrollArea"><div class="mediocre-tableEditable-tableWrapper" style="padding-left:0;"><div class="mediocre-tableEditable-border multi-editable" style="left:-10px;width:765px;"></div><table class="mediocre-tableEditable-table u-small-spacing-right" style="top:0;width:745px;"><colgroup><col style="width:45px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col><col style="width:100px;"></col></colgroup><tbody><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-maximizeButton u-no-mouse-target"></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">A</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">B</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">C</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">D</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">E</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">F</span></div></td><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--col"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">G</span></div></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">1</span></div></td><td class="cell is-text is-alignLeft" data-cid="table-note-part501-0-0" data-namespace="table-ns2038"><span>Tube #</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part501-0-1" data-namespace="table-ns2038"><span>dH2O (uL)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part501-0-2" data-namespace="table-ns2038"><span>10x cutsmart Buffer (uL)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part501-0-3" data-namespace="table-ns2038"><span>DNA approx 1 ug (uL)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part501-0-4" data-namespace="table-ns2038"><span>EcoRI (uL)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part501-0-5" data-namespace="table-ns2038"><span>SpeI (uL)</span></td><td class="cell is-text is-alignLeft" data-cid="table-note-part501-0-6" data-namespace="table-ns2038"><span>total volume (uL)</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">2</span></div></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-1-0" data-namespace="table-ns2038"><span>1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-1-1" data-namespace="table-ns2038"><span>18</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-1-2" data-namespace="table-ns2038"><span>2.5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-1-3" data-namespace="table-ns2038"><span>2.5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-1-4" data-namespace="table-ns2038"><span>1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-1-5" data-namespace="table-ns2038"><span>1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-1-6" data-namespace="table-ns2038"><span>25</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">3</span></div></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-2-0" data-namespace="table-ns2038"><span>2</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-2-1" data-namespace="table-ns2038"><span>17</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-2-2" data-namespace="table-ns2038"><span>2.5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-2-3" data-namespace="table-ns2038"><span>3.5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-2-4" data-namespace="table-ns2038"><span>1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-2-5" data-namespace="table-ns2038"><span>1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-2-6" data-namespace="table-ns2038"><span>25</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">4</span></div></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-3-0" data-namespace="table-ns2038"><span>3</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-3-1" data-namespace="table-ns2038"><span>16.35</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-3-2" data-namespace="table-ns2038"><span>2.5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-3-3" data-namespace="table-ns2038"><span>4</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-3-4" data-namespace="table-ns2038"><span>1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-3-5" data-namespace="table-ns2038"><span>1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-3-6" data-namespace="table-ns2038"><span>25</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">5</span></div></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-4-0" data-namespace="table-ns2038"><span>4</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-4-1" data-namespace="table-ns2038"><span>12.5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-4-2" data-namespace="table-ns2038"><span>2.5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-4-3" data-namespace="table-ns2038"><span>8</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-4-4" data-namespace="table-ns2038"><span>1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-4-5" data-namespace="table-ns2038"><span>1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-4-6" data-namespace="table-ns2038"><span>25</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">6</span></div></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-5-0" data-namespace="table-ns2038"><span>5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-5-1" data-namespace="table-ns2038"><span>9.5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-5-2" data-namespace="table-ns2038"><span>2.5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-5-3" data-namespace="table-ns2038"><span>11</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-5-4" data-namespace="table-ns2038"><span>1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-5-5" data-namespace="table-ns2038"><span>1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-5-6" data-namespace="table-ns2038"><span>25</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">7</span></div></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-6-0" data-namespace="table-ns2038"><span>6</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-6-1" data-namespace="table-ns2038"><span>11.5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-6-2" data-namespace="table-ns2038"><span>2.5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-6-3" data-namespace="table-ns2038"><span>9</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-6-4" data-namespace="table-ns2038"><span>1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-6-5" data-namespace="table-ns2038"><span>1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-6-6" data-namespace="table-ns2038"><span>25</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">8</span></div></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-7-0" data-namespace="table-ns2038"><span>7</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-7-1" data-namespace="table-ns2038"><span>6.5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-7-2" data-namespace="table-ns2038"><span>2.5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-7-3" data-namespace="table-ns2038"><span>14</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-7-4" data-namespace="table-ns2038"><span>1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-7-5" data-namespace="table-ns2038"><span>1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-7-6" data-namespace="table-ns2038"><span>25</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">9</span></div></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-8-0" data-namespace="table-ns2038"><span>8</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-8-1" data-namespace="table-ns2038"><span>5.5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-8-2" data-namespace="table-ns2038"><span>2.5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-8-3" data-namespace="table-ns2038"><span>15</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-8-4" data-namespace="table-ns2038"><span>1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-8-5" data-namespace="table-ns2038"><span>1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-8-6" data-namespace="table-ns2038"><span>25</span></td></tr><tr><td class="mediocre-tableEditable-axisCell mediocre-tableEditable-axisCell--row"><div class="mediocre-tableEditable-axisCell-numWrapper"><span class="mediocre-tableEditable-axisCell-num">10</span></div></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-9-0" data-namespace="table-ns2038"><span>9</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-9-1" data-namespace="table-ns2038"><span>10</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-9-2" data-namespace="table-ns2038"><span>2.5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-9-3" data-namespace="table-ns2038"><span>10.5</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-9-4" data-namespace="table-ns2038"><span>1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-9-5" data-namespace="table-ns2038"><span>1</span></td><td class="cell is-value is-alignRight" data-cid="table-note-part501-9-6" data-namespace="table-ns2038"><span>25</span></td></tr></tbody></table></div></div><div class="mediocre-tableEditable-titleBarWrapper" style="left:0;"><div class="mediocre-tableEditable-titleBar"><div class="u-ellipsis-text mediocre-tableEditable-tableName">Table13</div></div></div></div></div></div><div class="mediocre-captionEditable-cell"></div></div><div class="component-bottom-row component-row"><div class="mediocre-captionEditable-cell"></div><div class="mediocre-captionEditable-cell"><div class="mediocre-captionEditable-caption--bottom multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part501" data-index="262" style="width:720px;margin-left:0;">
-</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part502" data-index="263" style="margin-left:0;">Digested at 37°C for 2 hours, 80°C for 20 minutes, then to 4°C until next use. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part503" data-index="264" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part504" data-index="265" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 10/19</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part505" data-index="266" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part506" data-index="267" style="margin-left:0;">Run them on a Gel and see Results.</div></div></div></div></div><div class="dnd-drop-container-border"></div></div></div></div></div></div></div></div>
+</div></div><div class="mediocre-captionEditable-cell"></div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part502" data-index="263" style="margin-left:0;">Digested at 37°C for 2 hours, 80°C for 20 minutes, then to 4°C until next use. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part503" data-index="264" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part504" data-index="265" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 10/19</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part505" data-index="266" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part506" data-index="267" style="margin-left:0;">Run them on a Gel and see Results. Gel shows 2kb Vector band and 1.8kb Insert band, in and correct!</h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="https://static.igem.org/mediawiki/2016/2/2c/SCSU-New_HavenLastGEl.jpeg"/></div></div></div></div></div><div class="dnd-drop-container-border"></div></div></div></div></div></div></div></div>
+<a href="https://2016.igem.org/Team:SCSU-New_Haven" class="btn btn-info" role="button">Go Home!</a>
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Difference between revisions of "Team:SCSU-New Haven/Notebook"

Latest revision as of 22:04, 30 November 2016

SCSU iGEM Project work

10-1_iGEM_gel_pure_2016.10.01_10.14.15_Fl-UV.jpg

10-6_iGEMgel_ligmixs_2016.10.06_15.06.55_Fl-UV.jpg

10-14 VectorDigest 2016.10.14_15.18.11_Fl-UV.jpg