Difference between revisions of "Team:Goettingen/Notebook"

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                             <p><strong>6/2/15 </strong> - Had first official meeting to review the guidelines of the competition and begin brainstorming ideas for our project.</p>
+
                             <p>Katzen liegen nicht faul rum. Sie verschönern den Raum.</p>
−
                            <p><strong>6/2/15 </strong> - Regrouped to discuss ideas. Entertained diverse ideas from creating a pH sensing bacterium to use as a drug delivery system to fight cancer to using an enterobacterium that would function as a probiotic to try and suppress the Celiac’s disease response to gluten.</p>
+
                                                        <p>Katzen liegen nicht faul rum. Sie verschönern den Raum.</p>
−
                             <p><strong>6/2/15 </strong>- Decided that focusing on in vivo projects isn’t a good avenue for us to study because of the practical hurdles associated with trying to get an organism to live within the body and then to get that passed by the FDA. Thought about possibly trying to create some sort of helper cell that would aid in vitro screening processes, specifically for cancer research.</p>
+
                            
 
                     </div>
 
                     </div>
 
                         <div id="May_Week2" class="tab-pane fade">
 
                         <div id="May_Week2" class="tab-pane fade">
−
                             <p><strong>6/2/15 </strong> - Over the weekend we intensively studied immunotherapy and its cancer related applications. We thought about trying to create an organism that would maybe aid in the production of T and B-cells.</p>
+
                             <p>Katzen liegen nicht faul rum. Sie verschönern den Raum.</p>
−
                            <p><strong>6/2/15 </strong> - Decided that we didn’t have the proper resources to adequately test anything related to cancer research so we shifted gears. Some of us revisited the Celiac’s disease idea and tried finding a site in the recognition of gluten in an attempt to find a spot to repress the response. The rest then went back to the pH sensing system. We saw this competition as a driving force to construct more parts to expand the registry rather than to create a stand alone project, so creating a pH sensing system could prove useful in other applications.</p>
+
−
                            <p>5/16/15 - After more investigation we found that other pH sensors had been submitted to the Registry in the past but none were directly linked to transcription factors. So our focused turned to understanding nature’s way of sensing pH.</p>
+
 
                     </div>
 
                     </div>
 
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                         <div id="May_Week3" class="tab-pane fade">
−
                             <p><strong>6/2/15 </strong> - Reconvened to discuss findings. Only a few potential candidates were found but none seemed realistic. We decided to table the idea and keep brainstorming and return to this idea if we were unable to come up with anything else.</p>
+
                             <p>Katzen liegen nicht faul rum. Sie verschönern den Raum.</p>
−
                            <p><strong>6/2/15 </strong> - Entertained the idea of biofuel production. The team went an investigated potential molecules we would produce and the research that has already been done in this competition and out.</p>
+
 
                     </div>
 
                     </div>
 
                         <div id="May_Week4" class="tab-pane fade">
 
                         <div id="May_Week4" class="tab-pane fade">
−
                             <p><strong>6/2/15 </strong> - Started thinking more big picture about the goals of the synthetic biology and what kind of problems it can realistically solve at this point in time. We thought about how it is that such small organisms can have larger effects on society. This idea of scaling up biology bot us thinking about bioreactors.</p>
+
                             <p>Katzen liegen nicht faul rum. Sie verschönern den Raum.</p>
 
                     </div>
 
                     </div>
 
                     </div>
 
                     </div>
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                     <div id="June_Week1" class="tab-pane fade in active">
−
                             <p><strong>6/2/15 </strong>- With bioreactor on our mind, several of us took a field trip to Anaerobe Systems, a local company, to see the large scale fermentors that were in use there. After seeing their setup and how beneficial his systems were we shifted from bioreactors, in general, to fermentors that degrade organic waste like anaerobe systems does. None of us were familiar with the cellulolytic pathways so we went and did more research.</p>
+
                             <p>Katzen liegen nicht faul rum. Sie verschönern den Raum.</p>
 
                     </div>
 
                     </div>
 
                         <div id="June_Week2" class="tab-pane fade">
 
                         <div id="June_Week2" class="tab-pane fade">
−
                             <p><strong>6/2/15 </strong>- Met up to discuss everyone’s findings. We found that the exact mechanism for this degradation is still not well understood as it requires the use of a cellulosome, which is a conglomerate of numerous large proteins that has yet to be fully visualized. Our hope was to try and take several pivotal proteins from the complex and insert them into a high expression vector to see if by increasing the production of these proteins, we could increase the cellulolytic activity of the organism being used in the bioreactor.</p>
+
                             <p>Katzen liegen nicht faul rum. Sie verschönern den Raum.</p>
 
                     </div>
 
                     </div>
 
                         <div id="June_Week3" class="tab-pane fade">
 
                         <div id="June_Week3" class="tab-pane fade">
−
                             <p><strong>6/2/15 </strong> - Studied the actual workings of the fermentors at Anaerobe systems to see what problems were actually present. We found that the rate of activity of the organism was not the problem because they were using a cellulolytic <i>Clostridium</i> species, which has a doubling time of about 6 minutes. We did find, however, that their continuous culture system was unable to function for longer than three hours. We hypothesized this to be the result of the high acid concentration in the culture that accumulated over the time of the culture. In order to address this issue we decided to create a two part project: one side will sense low pH which would then induce the expression of the other side which is the regulating system that would be responsible for returning the intracellular pH to neutral.</p>
+
                             <p>Katzen liegen nicht faul rum. Sie verschönern den Raum.</p>
 
                     </div>
 
                     </div>
 
                      
 
                      
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                     <div id="July_Week1" class="tab-pane fade in active">
 
                     <div id="July_Week1" class="tab-pane fade in active">
−
                             <p><strong>6/2/15 </strong>- With bioreactor on our mind, several of us took a field trip to Anaerobe Systems, a local company, to see the large scale fermentors that were in use there. After seeing their setup and how beneficial his systems were we shifted from bioreactors, in general, to fermentors that degrade organic waste like anaerobe systems does. None of us were familiar with the cellulolytic pathways so we went and did more research.</p>
+
                             <p>Katzen liegen nicht faul rum. Sie verschönern den Raum.</p>
 
                     </div>
 
                     </div>
 
                         <div id="July_Week2" class="tab-pane fade">
 
                         <div id="July_Week2" class="tab-pane fade">
−
                             <p><strong>6/2/15 </strong>- Met up to discuss everyone’s findings. We found that the exact mechanism for this degradation is still not well understood as it requires the use of a cellulosome, which is a conglomerate of numerous large proteins that has yet to be fully visualized. Our hope was to try and take several pivotal proteins from the complex and insert them into a high expression vector to see if by increasing the production of these proteins, we could increase the cellulolytic activity of the organism being used in the bioreactor.</p>
+
                             <p>Katzen liegen nicht faul rum. Sie verschönern den Raum.</p>
 
                     </div>
 
                     </div>
 
                         <div id="July_Week3" class="tab-pane fade">
 
                         <div id="July_Week3" class="tab-pane fade">
−
                             <p><strong>6/2/15 </strong> - Studied the actual workings of the fermentors at Anaerobe systems to see what problems were actually present. We found that the rate of activity of the organism was not the problem because they were using a cellulolytic <i>Clostridium</i> species, which has a doubling time of about 6 minutes. We did find, however, that their continuous culture system was unable to function for longer than three hours. We hypothesized this to be the result of the high acid concentration in the culture that accumulated over the time of the culture. In order to address this issue we decided to create a two part project: one side will sense low pH which would then induce the expression of the other side which is the regulating system that would be responsible for returning the intracellular pH to neutral.</p>
+
                             <p>Katzen liegen nicht faul rum. Sie verschönern den Raum.</p>
 
                     </div>
 
                     </div>
 
                      
 
                      
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                     <div id="August_Week1" class="tab-pane fade in active">
−
                             <p><strong>6/2/15 </strong> - Had first official meeting to review the guidelines of the competition and begin brainstorming ideas for our project.</p>
+
                             <p>Katzen liegen nicht faul rum. Sie verschönern den Raum.</p>
−
                            <p><strong>6/2/15 </strong> - Regrouped to discuss ideas. Entertained diverse ideas from creating a pH sensing bacterium to use as a drug delivery system to fight cancer to using an enterobacterium that would function as a probiotic to try and suppress the Celiac’s disease response to gluten.</p>
+
−
                            <p><strong>6/2/15 </strong>- Decided that focusing on in vivo projects isn’t a good avenue for us to study because of the practical hurdles associated with trying to get an organism to live within the body and then to get that passed by the FDA. Thought about possibly trying to create some sort of helper cell that would aid in vitro screening processes, specifically for cancer research.</p>
+
 
                     </div>
 
                     </div>
 
                         <div id="August_Week2" class="tab-pane fade">
 
                         <div id="August_Week2" class="tab-pane fade">
−
                             <p><strong>6/2/15 </strong> - Over the weekend we intensively studied immunotherapy and its cancer related applications. We thought about trying to create an organism that would maybe aid in the production of T and B-cells.</p>
+
                             <p>Katzen liegen nicht faul rum. Sie verschönern den Raum.</p>
−
                            <p><strong>6/2/15 </strong> - Decided that we didn’t have the proper resources to adequately test anything related to cancer research so we shifted gears. Some of us revisited the Celiac’s disease idea and tried finding a site in the recognition of gluten in an attempt to find a spot to repress the response. The rest then went back to the pH sensing system. We saw this competition as a driving force to construct more parts to expand the registry rather than to create a stand alone project, so creating a pH sensing system could prove useful in other applications.</p>
+
−
                            <p>5/16/15 - After more investigation we found that other pH sensors had been submitted to the Registry in the past but none were directly linked to transcription factors. So our focused turned to understanding nature’s way of sensing pH.</p>
+
 
                     </div>
 
                     </div>
 
                         <div id="August_Week3" class="tab-pane fade">
 
                         <div id="August_Week3" class="tab-pane fade">
−
                             <p><strong>6/2/15 </strong> - Reconvened to discuss findings. Only a few potential candidates were found but none seemed realistic. We decided to table the idea and keep brainstorming and return to this idea if we were unable to come up with anything else.</p>
+
                             <p>Katzen liegen nicht faul rum. Sie verschönern den Raum.</p>
−
                            <p><strong>6/2/15 </strong> - Entertained the idea of biofuel production. The team went an investigated potential molecules we would produce and the research that has already been done in this competition and out.</p>
+
 
                     </div>
 
                     </div>
 
                         <div id="August_Week4" class="tab-pane fade">
 
                         <div id="August_Week4" class="tab-pane fade">
−
                             <p><strong>6/2/15 </strong> - Started thinking more big picture about the goals of the synthetic biology and what kind of problems it can realistically solve at this point in time. We thought about how it is that such small organisms can have larger effects on society. This idea of scaling up biology bot us thinking about bioreactors.</p>
+
                             <p>Katzen liegen nicht faul rum. Sie verschönern den Raum.</p>
 
                     </div>
 
                     </div>
 
                     </div>
 
                     </div>

Revision as of 17:11, 3 October 2016


Notebook


    May

    Katzen liegen nicht faul rum. Sie verschönern den Raum.

    Katzen liegen nicht faul rum. Sie verschönern den Raum.

    Katzen liegen nicht faul rum. Sie verschönern den Raum.

    Katzen liegen nicht faul rum. Sie verschönern den Raum.

    Katzen liegen nicht faul rum. Sie verschönern den Raum.

    June

    Katzen liegen nicht faul rum. Sie verschönern den Raum.

    Katzen liegen nicht faul rum. Sie verschönern den Raum.

    Katzen liegen nicht faul rum. Sie verschönern den Raum.

    July

    Katzen liegen nicht faul rum. Sie verschönern den Raum.

    Katzen liegen nicht faul rum. Sie verschönern den Raum.

    Katzen liegen nicht faul rum. Sie verschönern den Raum.

    August

    Katzen liegen nicht faul rum. Sie verschönern den Raum.

    Katzen liegen nicht faul rum. Sie verschönern den Raum.

    Katzen liegen nicht faul rum. Sie verschönern den Raum.

    Katzen liegen nicht faul rum. Sie verschönern den Raum.

Document the dates you worked on your project.

What should this page have?
  • Chronological notes of what your team is doing.
  • Brief descriptions of daily important events.
  • Pictures of your progress.
  • Mention who participated in what task.
Inspiration

You can see what others teams have done to organize their notes:


Contact


iGEM Team Göttingen 2016
Institut für Mikrobiologie
und Genetik
AG Prof. Dr. Rolf Daniel
Grisebachstr. 8
37077 Göttingen

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