Difference between revisions of "Team:Manchester/Parts"
Huiwenchong (Talk | contribs) |
Huiwenchong (Talk | contribs) |
||
| Line 64: | Line 64: | ||
<td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K2092004" target="_blank">http://parts.igem.org/Part:BBa_K2092004</a></td> | <td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K2092004" target="_blank">http://parts.igem.org/Part:BBa_K2092004</a></td> | ||
<td style="border:1px black solid;">alcR (incl. RBS)</td> | <td style="border:1px black solid;">alcR (incl. RBS)</td> | ||
| − | <td style="border:1px black solid;">This part is a twin of <a href="http://parts.igem.org/Part:BBa_K2092001" target="_blank">BBa_K2092001</a> with an additional <a href="http://parts.igem.org/Part:BBa_J34801" target="_blank"> | + | <td style="border:1px black solid;">This part is a twin of <a href="http://parts.igem.org/Part:BBa_K2092001" target="_blank">BBa_K2092001</a> with an additional RBS <a href="http://parts.igem.org/Part:BBa_J34801" target="_blank"> ( BBa_J34801)</a> upstream of the start codon. This part encodes for AlcR protein, an ethanol-activated transcriptional factor from <i>Aspergillus nidulans</i>. It binds to alcA (<a href="http://parts.igem.org/Part:BBa_K2092003" target="_blank">BBa_K2092002</a>) and aldA promoters to induce expression of proteins in the presence of ethanol or threonine.</td> |
<td style="border:1px black solid;">Composite</td> | <td style="border:1px black solid;">Composite</td> | ||
</tr> | </tr> | ||
Revision as of 23:03, 9 October 2016
Parts
| BioBrick | Link | Part Name | Description | Part Type |
|---|---|---|---|---|
| BBa_K2092000 | http://parts.igem.org/Part:BBa_K2092000 | AOx without RBS | This part encodes for the AOx1 protein from Pichia Pastoris. This enzyme oxidises primary alcohols to produce aldehydes and hydrogen peroxide. It is designed with an NDeI restriction site between the prefix and the start of the AOx gene. | Basic |
| BBa_K2092001 | http://parts.igem.org/Part:BBa_K2092001 | alcR without RBS/scar | This part encodes for AlcR protein, an ethanol-activated transcriptional factor. It binds to alcA promoter and aldA promoter to induce expression of proteins in the presence of ethanol or threonine. | Basic |
| BBa_K2092002 | http://parts.igem.org/Part:BBa_K2092002 | PalcA | This part contains the ethanol/alcR regulated alcA promoter, the native variant of alcA promoter from Aspergillus nidulans which tightly regulates gene expression. It is an improved BioBrick from 2011 iGEM DTU-Denmark BBa_K678001 | Basic |
| BBa_K2092003 | http://parts.igem.org/Part:BBa_K2092003 | PalcA(var) | This part contains the ethanol/alcR regulated alcA promoter, an engineered variant of the alcA promoter from Aspergillus nidulans which tightly regulates gene expression. The lack of one binding site on this promoter is suggested to have 70% decrease in promoter strength in comparison to the native BBa_K2092002. | Basic |
| BBa_K2092004 | http://parts.igem.org/Part:BBa_K2092004 | alcR (incl. RBS) | This part is a twin of BBa_K2092001 with an additional RBS ( BBa_J34801) upstream of the start codon. This part encodes for AlcR protein, an ethanol-activated transcriptional factor from Aspergillus nidulans. It binds to alcA (BBa_K2092002) and aldA promoters to induce expression of proteins in the presence of ethanol or threonine. | Composite |
| BBa_K2092006 | http://parts.igem.org/Part:BBa_K2092006 | CP3+RBS+amilCP | This part consists of a constitutive promoter (BBa_J23110), RBS (BBa_B0034) and blue chromoprotein amilCP (BBa_K592009). Suitable to be used as a reporter. | Composite |
| BBa_K2092008 | http://parts.igem.org/Part:BBa_K2092008 | CP3+RBS+alcR | This part consists of a constitutive promoter (BBa_J23110), RBS (BBa_B0034) and blue chromoprotein amilCP (BBa_K592009). Suitable to be used to measure the promoter strengths of alcA/aldA. | Composite |
| BBa_K2092009 | http://parts.igem.org/Part:BBa_K2092009 | PalcA var+RBS+amilCP | This part consists of PalcA var (BBa_K2092003) tagged with blue chromoprotein (BBa_K592009), which makes it capable to produce blue colour upon the binding of its ethanol-activated transcription factor AlcR. | Composite |
