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− | + | <h3 class="link"><a href="#overview">1. Overview</a></h3> | |
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− | + | <h3 class="link"><a href="#fitting">3. Fitting</a></h3> | |
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<p class="normal_text">Lastly we got and verified the desirable behavior of the fluorescence by modifying and improving them. | <p class="normal_text">Lastly we got and verified the desirable behavior of the fluorescence by modifying and improving them. | ||
As described below, it is consistent with the development of story.</p> | As described below, it is consistent with the development of story.</p> | ||
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Revision as of 10:35, 12 October 2016
![](https://static.igem.org/mediawiki/2016/f/f6/T--Tokyo_Tech--goTop.png)
Model
Contents
Overview
To reproduce the story of ”Snow White”, we have designed the cell-cell communication system with improved or characterized parts and collected data from comprehensive experiments. Furthermore, we constructed the mathematical model in order to simulate the behavior of the whole system and to confirm the feasibility of our story. This simulation successfully contributed to give the suggestions to wet lab experiments and confirm the feasibility of our system. In addition, in order to utilize TA (Toxin-Antitoxin) system, we developed a new software in Java for adjusting the number of ACA sequences, which MazF dimer recognizes and cleaves in mRNAs.
Mathematical Model
To simulate our gene circuits, we developed an ordinary differential equation model.
[Detailed Description for Modeling]
Analysis
Lastly we got and verified the desirable behavior of the fluorescence by modifying and improving them. As described below, it is consistent with the development of story.
SOFTWARE
ACA Sequence: