Line 10: | Line 10: | ||
− | < | + | <div> |
− | + | <div style="height:auto!important;margin-left:auto;margin-right:auto;min-height:841.9pt;padding-bottom:1pt;padding-top:1pt"> | |
− | </p> | + | <div style="margin-left:auto;margin-right:auto;position:relative"><p style="text-align:left"><a name="_GoBack"></a><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">Cry4Aa4+Extended FMDV 2A</span><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">-pChlamy_3<span></span></span></p> |
− | <p> | + | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0"> |
+ | <tbody><tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Name</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Cry4Aa4+Extended FMDV 2A<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Vector</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">pChlamy_3<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Construct Resistant</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Ampicillin in DH5</span><span style="font-family:Calibri;font-size:11.0pt">α</span><span style="font-family:Calibri;font-size:11.0pt"><span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Length</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">3673bp<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Strategy</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">XbaI and PstI<span></span></span></p> | ||
+ | </td> </tr></tbody> | ||
+ | </table> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0"> | ||
+ | <tbody><tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">QC Items</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Method</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Specifications</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Results</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Target Sequence<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequencing results are consistent to the confirmed sequence.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Vector Sequence<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">20bp flanking sequences of the vector are correct.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Reading Frame<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Frame is correct and consistent to the client's requirement.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Fragment Size<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Restriction Digests<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">The size of inserted fragment is right and free of any contaminated bands.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Quality/Quantity<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">UV spectrophotometry<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Miniprep: OD260/280=1.7~2.0<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">2</span><span style="font-family:Calibri;font-size:11.0pt">µ</span><span style="font-family:Calibri;font-size:11.0pt">g/tube, 1 tube,Lyophilized<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Type of Strain Stock<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Bacteria stock.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Appearance<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Clear and free of foreign particles, and the strain is clearly visible.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr></tbody> | ||
+ | </table> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0"> | ||
+ | <tbody><tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="263.20001220703125" src="http://b263.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/yfm7rDaljwf3tLDwN85uBcwarSLNpdnfMlUxh1G7pP4!/b/dAcBAAAAAAAA&bo=sAAIAQAAAAADAJw! " width="175.1999969482422"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="294.3999938964844" src="http://b198.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/O5V87mM7vgBQcBKlBbjOuO*vaJzRp9ohwsGNYf368uI!/b/dMYAAAAAAAAA&bo=nQAnAQAAAAADAJ4! " width="156.0"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane M: DNA Marker<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 1: Plasmid digested by XbaI and PstI <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 2: Plasmid DNA <span></span></span></p> | ||
+ | </td> </tr></tbody> | ||
+ | </table> | ||
+ | <p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold"> </span></p> | ||
+ | <p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold"> </span></p> | ||
+ | <p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">Cry4Aa4+Extended FMDV 2A</span><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">-pChlamy_3<span></span></span></p> | ||
− | < | + | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0"> |
− | < | + | <tbody><tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Name</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> |
− | <p> | + | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Cry10Aa4+Extended FMDV 2A<span></span></span></p> |
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Vector</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">pChlamy_3<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Construct Resistant</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Ampicillin in Top10<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Length</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">2158bp<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Strategy</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">XbaI and PstI<span></span></span></p> | ||
+ | </td> </tr></tbody> | ||
+ | </table> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0"> | ||
+ | <tbody><tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">QC Items</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Method</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Specifications</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Results</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Target Sequence<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequencing results are consistent to the confirmed sequence.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Vector Sequence<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">20bp flanking sequences of the vector are correct.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Reading Frame<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Frame is correct and consistent to the client's requirement.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Fragment Size<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Restriction Digests<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">The size of inserted fragment is right and free of any contaminated bands.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Quality/Quantity<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">UV spectrophotometry<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Miniprep: OD260/280=1.7~2.0<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">2</span><span style="font-family:Calibri;font-size:11.0pt">µ</span><span style="font-family:Calibri;font-size:11.0pt">g/tube, 1 tube,Lyophilized<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Type of Strain Stock<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Bacteria stock.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Appearance<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Clear and free of foreign particles, and the strain is clearly visible.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr></tbody> | ||
+ | </table> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | |||
+ | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0"> | ||
+ | <tbody><tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="254.39999389648438" src="http://b262.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/bQ8OOekpGDUmt.8vkQvJx0XUpn6PMh1VgaiF0dasSRg!/b/dAYBAAAAAAAA&bo=sAD*AAAAAAADAGo! " width="175.1999969482422"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="308.0" src="http://b199.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/LjSeZIndHm9zkUE6gT36bEx3VnYrEwCbYS7Aq.zBlqw!/b/dMcAAAAAAAAA&bo=7QA1AQAAAAADAPw! " width="236.8000030517578"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane M: DNA Marker<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 1: Plasmid digested by XbaI and PstI<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 2: Plasmid DNA <span></span></span></p> | ||
+ | </td> </tr></tbody> | ||
+ | </table> | ||
+ | <p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold"> </span></p> | ||
+ | <p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold"> </span></p> | ||
+ | <p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">Cry11Aa4+ Extended FMDV 2A</span><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">-pChlamy_3<span></span></span></p> | ||
+ | |||
+ | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0"> | ||
+ | <tbody><tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Name</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Cry11Aa4+ Extended FMDV 2A<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Vector</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">pChlamy_3<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Construct Resistant</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Ampicillin in JM108<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Length</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">2071bp<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Strategy</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">XbaI and PstI<span></span></span></p> | ||
+ | </td> </tr></tbody> | ||
+ | </table> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | |||
+ | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0"> | ||
+ | <tbody><tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">QC Items</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Method</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Specifications</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Results</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Target Sequence<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequencing results are consistent to the confirmed sequence.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Vector Sequence<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">20bp flanking sequences of the vector are correct.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Reading Frame<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Frame is correct and consistent to the client's requirement.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Fragment Size<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Restriction Digests<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">The size of inserted fragment is right and free of any contaminated bands.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Quality/Quantity<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">UV spectrophotometry<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Miniprep: OD260/280=1.7~2.0<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">2</span><span style="font-family:Calibri;font-size:11.0pt">µ</span><span style="font-family:Calibri;font-size:11.0pt">g/tube, 1 tube,Lyophilized<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Type of Strain Stock<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Bacteria stock.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Appearance<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Clear and free of foreign particles, and the strain is clearly visible.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr></tbody> | ||
+ | </table> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | |||
+ | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0"> | ||
+ | <tbody><tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="254.39999389648438" src="http://b198.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/d0.upRVhjL7OQjfkLGru*.dUocUXc4fDAJpeQEXjZe8!/b/dMYAAAAAAAAA&bo=sAD*AAAAAAADAGo! " width="175.1999969482422"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="308.0" src="http://b198.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/0MvndoK9mmRt.kJuTifErWbDLJSPJui*8L*GLlyeGl8!/b/dMYAAAAAAAAA&bo=qAA1AQAAAAADALk! " width="167.1999969482422"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane M: DNA Marker<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 1: Plasmid digested by XbaI and PstI<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 2: Plasmid DNA <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#595959;font-family:Calibri;font-size:9.0pt;font-style:italic"> </span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> </tr></tbody> | ||
+ | </table> | ||
+ | <p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold"> </span></p> | ||
+ | <p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold"> </span></p> | ||
+ | <p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">Cyt1-pChlamy_3<span></span></span></p> | ||
+ | |||
+ | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0"> | ||
+ | <tbody><tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Name</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Cyt1<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Vector</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">pChlamy_3<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Construct Resistant</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Ampicillin in JM108<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Length</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">762bp<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Strategy</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">XbaI and PstI<span></span></span></p> | ||
+ | </td> </tr></tbody> | ||
+ | </table> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | |||
+ | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0"> | ||
+ | <tbody><tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">QC Items</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Method</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Specifications</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Results</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Target Sequence<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequencing results are consistent to the confirmed sequence.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Vector Sequence<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">20bp flanking sequences of the vector are correct.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Reading Frame<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Frame is correct and consistent to the client's requirement.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Fragment Size<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Restriction Digests<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">The size of inserted fragment is right and free of any contaminated bands.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Quality/Quantity<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">UV spectrophotometry<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Miniprep: OD260/280=1.7~2.0<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">2</span><span style="font-family:Calibri;font-size:11.0pt">µ</span><span style="font-family:Calibri;font-size:11.0pt">g/tube, 1 tube,Lyophilized<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Type of Strain Stock<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Bacteria stock.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Appearance<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Clear and free of foreign particles, and the strain is clearly visible.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr></tbody> | ||
+ | </table> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | |||
+ | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0"> | ||
+ | <tbody><tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="254.39999389648438" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/Sy.SKFU5edTQSIF.h2uwVTZjSgxUj3zmtAOCAGbAaZo!/b/dMgAAAAAAAAA&bo=sAD*AAAAAAADAGo! " width="175.1999969482422"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="279.20001220703125" src="http://b264.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/xncHHhLWQz1aqSpaH4UR7R5bMZ4zFZ*PmV1nZxwVTQ8!/b/dAgBAAAAAAAA&bo=sAAYAQAAAAADAIw! " width="175.1999969482422"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane M: DNA Marker<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 1: Plasmid digested by XbaI and PstI<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 2: Plasmid DNA <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#595959;font-family:Calibri;font-size:9.0pt;font-style:italic"> </span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | </td> </tr></tbody> | ||
+ | </table> | ||
+ | <p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold"> </span></p> | ||
+ | <p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold"> </span></p> | ||
+ | <p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">Cyt2-pChlamy_3<span></span></span></p> | ||
+ | |||
+ | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0"> | ||
+ | <tbody><tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Name</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Cyt1<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Vector</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">pChlamy_3<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Construct Resistant</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Ampicillin in JM108<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Length</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">801bp<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Strategy</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">XbaI and PstI<span></span></span></p> | ||
+ | </td> </tr></tbody> | ||
+ | </table> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | |||
+ | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0"> | ||
+ | <tbody><tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">QC Items</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Method</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Specifications</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Results</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Target Sequence<span></span></span></p> | ||
+ | </td> <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p> | ||
+ | </td> <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequencing results are consistent to the confirmed sequence.<span></span></span></p> | ||
+ | </td> <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Vector Sequence<span></span></span></p> | ||
+ | </td> <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p> | ||
+ | </td> <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">20bp flanking sequences of the vector are correct.<span></span></span></p> | ||
+ | </td> <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Reading Frame<span></span></span></p> | ||
+ | </td> <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p> | ||
+ | </td> <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Frame is correct and consistent to the client's requirement.<span></span></span></p> | ||
+ | </td> <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Fragment Size<span></span></span></p> | ||
+ | </td> <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Restriction Digests<span></span></span></p> | ||
+ | </td> <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">The size of inserted fragment is right and free of any contaminated bands.<span></span></span></p> | ||
+ | </td> <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Quality/Quantity<span></span></span></p> | ||
+ | </td> <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">UV spectrophotometry<span></span></span></p> | ||
+ | </td> <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Miniprep: OD260/280=1.7~2.0<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">2</span><span style="font-family:Calibri;font-size:11.0pt">µ</span><span style="font-family:Calibri;font-size:11.0pt">g/tube, 1 tube,Lyophilized<span></span></span></p> | ||
+ | </td> <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Type of Strain Stock<span></span></span></p> | ||
+ | </td> <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p> | ||
+ | </td> <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Bacteria stock.<span></span></span></p> | ||
+ | </td> <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Appearance<span></span></span></p> | ||
+ | </td> <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p> | ||
+ | </td> <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Clear and free of foreign particles, and the strain is clearly visible.<span></span></span></p> | ||
+ | </td> <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="254.39999389648438" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/d0mowbkc0M494OfDGRHIGtmKGQNkXPPWleN7nPIwSf0!/b/dMgAAAAAAAAA&bo=sAD*AAAAAAADAGo! " width="175.1999969482422"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | </td> <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="280.0" src="http://b262.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/irbPRy7*zARuesyKRBXZFk6ZWMGm44ZwwyjWYNaqYZA!/b/dAYBAAAAAAAA&bo=4QAZAQAAAAADANw! " width="224.0"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane M: DNA Marker<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 1: Plasmid digested by XbaI and PstI<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 2: Plasmid DNA <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | </td> <td style="border:none;padding:0pt 0pt 0pt 0pt" width="54"> <p> </p> | ||
+ | </td> </tr> <tr height="0"> <td style="border:none" width="138"></td> <td style="border:none" width="45"></td> <td style="border:none" width="124"></td> <td style="border:none" width="107"></td> <td style="border:none" width="80"></td> <td style="border:none" width="2"></td> <td style="border:none" width="54"></td> </tr> </tbody> | ||
+ | </table> | ||
+ | <p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold"> </span></p> | ||
+ | <p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold"> </span></p> | ||
+ | <p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold"> </span></p> | ||
+ | <p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">EGFP-pChlamy_3<span></span></span></p> | ||
+ | |||
+ | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0"> | ||
+ | <tbody><tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Name</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">06-EGFP</span><span style="font-family:SimSun;font-size:11.0pt">(</span><span style="font-family:Calibri;font-size:11.0pt">TAA</span><span style="font-family:SimSun;font-size:11.0pt">)</span><span style="font-family:SimSun;font-size:11.0pt"><span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Vector</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">pChlamy_3<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Construct Resistant</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Ampicillin in Top10<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Length</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">732bp<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Strategy</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">XbaI and PstI<span></span></span></p> | ||
+ | </td> </tr></tbody> | ||
+ | </table> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | |||
+ | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0"> | ||
+ | <tbody><tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">QC Items</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Method</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Specifications</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Results</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Target Sequence<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequencing results are consistent to the confirmed sequence.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Vector Sequence<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">20bp flanking sequences of the vector are correct.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Reading Frame<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Frame is correct and consistent to the client's requirement.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Fragment Size<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Restriction Digests<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">The size of inserted fragment is right and free of any contaminated bands.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Quality/Quantity<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">UV spectrophotometry<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Miniprep: OD260/280=1.7~2.0<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">2</span><span style="font-family:Calibri;font-size:11.0pt">µ</span><span style="font-family:Calibri;font-size:11.0pt">g/tube, 1 tube,Lyophilized<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Type of Strain Stock<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Bacteria stock.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Appearance<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Clear and free of foreign particles, and the strain is clearly visible.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr></tbody> | ||
+ | </table> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | |||
+ | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0"> | ||
+ | <tbody><tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="254.39999389648438" src="http://b260.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/kV2mqmj1q8v1ump215nFHpnjMUzwxqSu0DronaWKuIs!/b/dAQBAAAAAAAA&bo=sAD*AAAAAAADAGo! " width="175.1999969482422"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="296.0" src="http://b199.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/aRGJqrgiOwOJDeH5hDKr.6c7zKU8VMfcgFHbm35PZ6I!/b/dMcAAAAAAAAA&bo=8AApAQAAAAADAP0! " width="239.1999969482422"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane M: DNA Marker<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 1: Plasmid digested by XbaI and PstI<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 2: Plasmid DNA <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | </td> </tr></tbody> | ||
+ | </table> | ||
+ | <p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold"> </span></p> | ||
+ | <p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">Cry4Aa4+Extended2A+Cyt2-pChlamy3<span></span></span></p> | ||
+ | |||
+ | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0"> | ||
+ | <tbody><tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Name</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Cry4Aa4+Extended2A+Cyt2<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Vector</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">pChlamy_3<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Construct Resistant</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Ampicillin in Top10<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Length</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">4461bp<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Strategy</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">XbaI and PstI<span></span></span></p> | ||
+ | </td> </tr></tbody> | ||
+ | </table> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | |||
+ | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0"> | ||
+ | <tbody><tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">QC Items</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Method</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Specifications</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Results</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Target Sequence<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequencing results are consistent to the confirmed sequence.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Vector Sequence<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">20bp flanking sequences of the vector are correct.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Reading Frame<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Frame is correct and consistent to the client's requirement.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Fragment Size<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Restriction Digests<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">The size of inserted fragment is right and free of any contaminated bands.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Quality/Quantity<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">UV spectrophotometry<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Miniprep: OD260/280=1.7~2.0<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">2</span><span style="font-family:Calibri;font-size:11.0pt">µ</span><span style="font-family:Calibri;font-size:11.0pt">g/tube, 1 tube,Lyophilized<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Type of Strain Stock<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Bacteria stock.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Appearance<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Clear and free of foreign particles, and the strain is clearly visible.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr></tbody> | ||
+ | </table> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"><span> </span><span></span></span></p> | ||
+ | |||
+ | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0"> | ||
+ | <tbody><tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="254.39999389648438" src="http://b199.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/uN*b2mkL9gaipMv8XpN.4wpt8raBNnOQuGwbKC.w*vE!/b/dMcAAAAAAAAA&bo=qQD*AAAAAAADAHM! " width="168.8000030517578"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="236.0" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/JI.J1CeIa*MhQu9aafo8T8eZeOr7zqxlK5q6W3NjbEA!/b/dMgAAAAAAAAA&bo=mADtAAAAAAADAFA! " width="151.1999969482422"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"><span> </span><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane M: DNA Marker<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 1: Plasmid digested by XbaI and BamHI<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 2: Plasmid DNA <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#595959;font-family:Calibri;font-size:9.0pt;font-style:italic"><span> </span></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | </td> </tr></tbody> | ||
+ | </table> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> </span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> </span></p> | ||
+ | <p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">Cry4Aa4+Extended2A+Cyt1-pChlamy3<span></span></span></p> | ||
+ | |||
+ | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0"> | ||
+ | <tbody><tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Name</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Cry4Aa4+Extended2A+Cyt1-pChlamy3<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Vector</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">pChlamy_3<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Construct Resistant</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Ampicillin in JM108<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Length</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">4422bp<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Strategy</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">XbaI and PstI<span></span></span></p> | ||
+ | </td> </tr></tbody> | ||
+ | </table> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | |||
+ | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0"> | ||
+ | <tbody><tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">QC Items</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Method</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td colspan="3" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Specifications</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Results</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Target Sequence<span></span></span></p> | ||
+ | </td> <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p> | ||
+ | </td> <td colspan="3" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequencing results are consistent to the confirmed sequence.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Vector Sequence<span></span></span></p> | ||
+ | </td> <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p> | ||
+ | </td> <td colspan="3" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">20bp flanking sequences of the vector are correct.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Reading Frame<span></span></span></p> | ||
+ | </td> <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p> | ||
+ | </td> <td colspan="3" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Frame is correct and consistent to the client's requirement.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Fragment Size<span></span></span></p> | ||
+ | </td> <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Restriction Digests<span></span></span></p> | ||
+ | </td> <td colspan="3" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">The size of inserted fragment is right and free of any contaminated bands.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Quality/Quantity<span></span></span></p> | ||
+ | </td> <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">UV spectrophotometry<span></span></span></p> | ||
+ | </td> <td colspan="3" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Miniprep: OD260/280=1.7~2.0<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">2</span><span style="font-family:Calibri;font-size:11.0pt">µ</span><span style="font-family:Calibri;font-size:11.0pt">g/tube, 1 tube,Lyophilized<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Type of Strain Stock<span></span></span></p> | ||
+ | </td> <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p> | ||
+ | </td> <td colspan="3" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Bacteria stock.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Appearance<span></span></span></p> | ||
+ | </td> <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p> | ||
+ | </td> <td colspan="3" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Clear and free of foreign particles, and the strain is clearly visible.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="254.39999389648438" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/pDvRNLD368siSdiKOdhck0A80upc24m2Aqj5qn.yyfY!/b/dMgAAAAAAAAA&bo=qQD*AAAAAAADAHM! " width="168.8000030517578"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="280.0" src="http://b199.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/LqdjCnlIs0z8YJ*6kbyj5ACC8gGPT6IlK2OdWcxc4T0!/b/dMcAAAAAAAAA&bo=rgAZAQAAAAADAJM! " width="173.60000610351562"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane M: DNA Marker<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 1: Plasmid digested by BamHI and KpnI<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 2: Plasmid DNA <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#595959;font-family:Calibri;font-size:9.0pt;font-style:italic"> </span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | </td> <td colspan="2" style="border:none;padding:0pt 0pt 0pt 0pt" width="63"> <p> </p> | ||
+ | </td> </tr> <tr height="0"> <td style="border:none" width="137"></td> <td style="border:none" width="39"></td> <td style="border:none" width="132"></td> <td style="border:none" width="51"></td> <td style="border:none" width="128"></td> <td style="border:none" width="7"></td> <td style="border:none" width="56"></td> </tr> </tbody> | ||
+ | </table> | ||
+ | <p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">Cry10Aa4+Extended2A+Cyt2-pChlamy3<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | |||
+ | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0"> | ||
+ | <tbody><tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Name</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Cry10Aa4+Extended2A+Cyt2-pChlamy3<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Vector</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">pChlamy_3<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Construct Resistant</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Ampicillin in DH5</span><span style="font-family:Calibri;font-size:11.0pt">α</span><span style="font-family:Calibri;font-size:11.0pt"><span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Length</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">2946bp<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Strategy</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">XbaI and PstI<span></span></span></p> | ||
+ | </td> </tr></tbody> | ||
+ | </table> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | |||
+ | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0"> | ||
+ | <tbody><tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">QC Items</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Method</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Specifications</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Results</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Target Sequence<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequencing results are consistent to the confirmed sequence.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Vector Sequence<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">20bp flanking sequences of the vector are correct.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Reading Frame<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Frame is correct and consistent to the client's requirement.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Fragment Size<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Restriction Digests<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">The size of inserted fragment is right and free of any contaminated bands.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Quality/Quantity<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">UV spectrophotometry<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Miniprep: OD260/280=1.7~2.0<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">2</span><span style="font-family:Calibri;font-size:11.0pt">µ</span><span style="font-family:Calibri;font-size:11.0pt">g/tube, 1 tube,Lyophilized<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Type of Strain Stock<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Bacteria stock.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Appearance<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Clear and free of foreign particles, and the strain is clearly visible.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr></tbody> | ||
+ | </table> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | |||
+ | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0"> | ||
+ | <tbody><tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="254.39999389648438" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/H1*GSbsbhQbTR1eeNDiS8fA5AfLGIser.g5DKjHyGtU!/b/dMgAAAAAAAAA&bo=sAD*AAAAAAADAGo! " width="175.1999969482422"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="264.0" src="http://b198.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/JfiiusuOJGnfyciEpeNFbNeC5oqcVaFFkIBoPRxw6x0!/b/dMYAAAAAAAAA&bo=jQAJAQAAAAADAKA! " width="140.0"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane M: DNA Marker<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 1: Plasmid digested by XbaI and PstI<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 2: Plasmid DNA <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#595959;font-family:Calibri;font-size:9.0pt;font-style:italic"> </span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | </td> </tr></tbody> | ||
+ | </table> | ||
+ | <p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold"> </span></p> | ||
+ | <p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold"> </span></p> | ||
+ | <p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">C</span><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">ry</span><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">10Aa4+Extended2A+Cyt2-pChlamy3<span></span></span></p> | ||
+ | |||
+ | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0"> | ||
+ | <tbody><tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Name</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Cry10Aa4+Extended2A+Cyt2-pChlamy3<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Vector</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">pChlamy_3<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Construct Resistant</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Ampicillin in DH5</span><span style="font-family:Calibri;font-size:11.0pt">α</span><span style="font-family:Calibri;font-size:11.0pt"><span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Length</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">2946bp<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Strategy</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">XbaI and PstI<span></span></span></p> | ||
+ | </td> </tr></tbody> | ||
+ | </table> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | |||
+ | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0"> | ||
+ | <tbody><tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">QC Items</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Method</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Specifications</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Results</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Target Sequence<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequencing results are consistent to the confirmed sequence.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Vector Sequence<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">20bp flanking sequences of the vector are correct.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Reading Frame<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Frame is correct and consistent to the client's requirement.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Fragment Size<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Restriction Digests<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">The size of inserted fragment is right and free of any contaminated bands.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Quality/Quantity<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">UV spectrophotometry<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Miniprep: OD260/280=1.7~2.0<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">2</span><span style="font-family:Calibri;font-size:11.0pt">µ</span><span style="font-family:Calibri;font-size:11.0pt">g/tube, 1 tube,Lyophilized<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Type of Strain Stock<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Bacteria stock.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Appearance<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Clear and free of foreign particles, and the strain is clearly visible.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr></tbody> | ||
+ | </table> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | |||
+ | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0"> | ||
+ | <tbody><tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="254.39999389648438" src="http://b199.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/9p5OCN4X0ji10gEBq68TdCUN93aBGaDnJIBTc5E0f3M!/b/dMcAAAAAAAAA&bo=sAD*AAAAAAADAGo! " width="175.1999969482422"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="273.6000061035156" src="http://b259.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/1zft.p5lOoX2yVoxccDyfQWcQjbGRb2SD*Zq97vSedo!/b/dAMBAAAAAAAA&bo=kQASAQAAAAADAKc! " width="144.8000030517578"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane M: DNA Marker<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 1: Plasmid digested by XbaI and PstI<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 2: Plasmid DNA <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#595959;font-family:Calibri;font-size:9.0pt;font-style:italic"> </span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | </td> </tr></tbody> | ||
+ | </table> | ||
+ | <p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">Cry10Aa4+Extended2A+Cyt1-pChlamy3 <span></span></span></p> | ||
+ | |||
+ | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0"> | ||
+ | <tbody><tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Name</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Cry10Aa4+Extended2A+Cyt1-pChlamy3<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Vector</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">pChlamy_3<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Construct Resistant</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Ampicillin in DH5</span><span style="font-family:Calibri;font-size:11.0pt">α</span><span style="font-family:Calibri;font-size:11.0pt"><span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Length</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">2907bp<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Strategy</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">XbaI and PstI<span></span></span></p> | ||
+ | </td> </tr></tbody> | ||
+ | </table> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | |||
+ | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0"> | ||
+ | <tbody><tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">QC Items</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Method</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td colspan="3" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Specifications</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Results</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Target Sequence<span></span></span></p> | ||
+ | </td> <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p> | ||
+ | </td> <td colspan="3" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequencing results are consistent to the confirmed sequence.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Vector Sequence<span></span></span></p> | ||
+ | </td> <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p> | ||
+ | </td> <td colspan="3" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">20bp flanking sequences of the vector are correct.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Reading Frame<span></span></span></p> | ||
+ | </td> <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p> | ||
+ | </td> <td colspan="3" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Frame is correct and consistent to the client's requirement.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Fragment Size<span></span></span></p> | ||
+ | </td> <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Restriction Digests<span></span></span></p> | ||
+ | </td> <td colspan="3" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">The size of inserted fragment is right and free of any contaminated bands.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Quality/Quantity<span></span></span></p> | ||
+ | </td> <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">UV spectrophotometry<span></span></span></p> | ||
+ | </td> <td colspan="3" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Miniprep: OD260/280=1.7~2.0<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">2</span><span style="font-family:Calibri;font-size:11.0pt">µ</span><span style="font-family:Calibri;font-size:11.0pt">g/tube, 1 tube,Lyophilized<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Type of Strain Stock<span></span></span></p> | ||
+ | </td> <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p> | ||
+ | </td> <td colspan="3" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Bacteria stock.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Appearance<span></span></span></p> | ||
+ | </td> <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p> | ||
+ | </td> <td colspan="3" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Clear and free of foreign particles, and the strain is clearly visible.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="254.39999389648438" src="http://b262.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/qchMjMQfSkqMMyaq5LhKw3ZuaGXoJRnrEvO0Slo0S1A!/b/dAYBAAAAAAAA&bo=sAD*AAAAAAADAGo! " width="175.1999969482422"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | </td> <td colspan="2" style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="288.79998779296875" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/Rme26s7JjvB5bA9BlIrNu00W2reMmKwHkx9yaus6ikU!/b/dMgAAAAAAAAA&bo=zQAhAQAAAAADAMg! " width="204.8000030517578"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane M: DNA Marker<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 1: Plasmid digested by XbaI and PstI<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 2: Plasmid DNA <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | </td> <td colspan="2" style="border:none;padding:0pt 0pt 0pt 0pt" width="60"> <p> </p> | ||
+ | </td> </tr> <tr height="0"> <td style="border:none" width="135"></td> <td style="border:none" width="45"></td> <td style="border:none" width="131"></td> <td style="border:none" width="78"></td> <td style="border:none" width="101"></td> <td style="border:none" width="5"></td> <td style="border:none" width="55"></td> </tr> </tbody> | ||
+ | </table> | ||
+ | <p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">Cry11Aa4+Extended2A+Cyt2-pChlamy3<span></span></span></p> | ||
+ | |||
+ | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0"> | ||
+ | <tbody><tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Name</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Cry11Aa4+Extended2A+Cyt2-pChlamy3<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Vector</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">pChlamy_3<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Construct Resistant</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Ampicillin in Top10<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Length</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">2859bp<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Strategy</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">XbaI and PstI<span></span></span></p> | ||
+ | </td> </tr></tbody> | ||
+ | </table> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | |||
+ | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0"> | ||
+ | <tbody><tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">QC Items</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Method</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Specifications</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Results</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Target Sequence<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequencing results are consistent to the confirmed sequence.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Vector Sequence<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">20bp flanking sequences of the vector are correct.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Reading Frame<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Frame is correct and consistent to the client's requirement.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Fragment Size<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Restriction Digests<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">The size of inserted fragment is right and free of any contaminated bands.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Quality/Quantity<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">UV spectrophotometry<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Miniprep: OD260/280=1.7~2.0<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">2</span><span style="font-family:Calibri;font-size:11.0pt">µ</span><span style="font-family:Calibri;font-size:11.0pt">g/tube, 1 tube,Lyophilized<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Type of Strain Stock<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Bacteria stock.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Appearance<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Clear and free of foreign particles, and the strain is clearly visible.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr></tbody> | ||
+ | </table> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | |||
+ | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0"> | ||
+ | <tbody><tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="254.39999389648438" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/0LciDoc2Vues3GO6fYi7EnNqrsVS8mSF.qFDZziDpEA!/b/dMgAAAAAAAAA&bo=sAD*AAAAAAADAGo! " width="175.1999969482422"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="258.3999938964844" src="http://b198.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/hJNHsLw6HFbz9eR7eszEpW*U.RfWwyhYSlkMIPiWAbc!/b/dMYAAAAAAAAA&bo=wwADAQAAAAADAOQ! " width="194.39999389648438"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane M: DNA Marker<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 1: Plasmid digested by </span><span style="font-family:Calibri;font-size:11.0pt"> XbaI and PstI</span><span style="font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 2: Plasmid DNA <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | </td> </tr></tbody> | ||
+ | </table> | ||
+ | <p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">Cry11Aa4+Extended2A+Cyt1-pChlamy3<span></span></span></p> | ||
+ | |||
+ | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0"> | ||
+ | <tbody><tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Name</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Cry11Aa4+Extended2A+Cyt1-pChlamy3<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Vector</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">pChlamy_3<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Construct Resistant</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Ampicillin in JM108<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Length</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">2820bp<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Strategy</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">XbaI and PstI<span></span></span></p> | ||
+ | </td> </tr></tbody> | ||
+ | </table> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | |||
+ | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0"> | ||
+ | <tbody><tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">QC Items</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Method</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Specifications</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Results</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Target Sequence<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequencing results are consistent to the confirmed sequence.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Vector Sequence<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">20bp flanking sequences of the vector are correct.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Reading Frame<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Frame is correct and consistent to the client's requirement.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Fragment Size<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Restriction Digests<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">The size of inserted fragment is right and free of any contaminated bands.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Quality/Quantity<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">UV spectrophotometry<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Miniprep: OD260/280=1.7~2.0<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">2</span><span style="font-family:Calibri;font-size:11.0pt">µ</span><span style="font-family:Calibri;font-size:11.0pt">g/tube, 1 tube,Lyophilized<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Type of Strain Stock<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Bacteria stock.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Appearance<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Clear and free of foreign particles, and the strain is clearly visible.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr></tbody> | ||
+ | </table> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | |||
+ | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0"> | ||
+ | <tbody><tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="254.39999389648438" src="http://b264.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/.39If2pAeG1h2OXRhp49InOl3WTN1asUm8AXJFwwqZs!/b/dAgBAAAAAAAA&bo=sAD*AAAAAAADAGo! " width="175.1999969482422"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="264.0" src="http://b200.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/pZ5hAlXd5CMg3MJ.c2OE2MHz2ke4Ev3fW3jyPfdOUPU!/b/dMgAAAAAAAAA&bo=vQAJAQAAAAADAJA! " width="188.0"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane M: DNA Marker<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 1: Plasmid digested by XbaI and PstI<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 2: Plasmid DNA <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#595959;font-family:Calibri;font-size:9.0pt;font-style:italic"> </span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | </td> </tr></tbody> | ||
+ | </table> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#1E4E79;font-family:Calibri;font-size:16.0pt;font-weight:bold">Cry11Aa4+Extended2A+EGFP-pChlamy3<span></span></span></p> | ||
+ | |||
+ | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0"> | ||
+ | <tbody><tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Name</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Cry11Aa4+Extended2A+EGFP-pChlamy3<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Vector</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">pChlamy_3<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Construct Resistant</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Ampicillin in JM108<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Gene Length</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">2790bp<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Cloning Strategy</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">XbaI and PstI<span></span></span></p> | ||
+ | </td> </tr></tbody> | ||
+ | </table> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | |||
+ | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0"> | ||
+ | <tbody><tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">QC Items</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Method</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Specifications</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:12.0pt;font-weight:bold">Results</span><span style="color:#000000;font-family:Calibri;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Target Sequence<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequencing results are consistent to the confirmed sequence.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Vector Sequence<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">20bp flanking sequences of the vector are correct.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Reading Frame<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Sequence alignment<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Frame is correct and consistent to the client's requirement.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Fragment Size<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Restriction Digests<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">The size of inserted fragment is right and free of any contaminated bands.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Quality/Quantity<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">UV spectrophotometry<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Miniprep: OD260/280=1.7~2.0<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">2</span><span style="font-family:Calibri;font-size:11.0pt">µ</span><span style="font-family:Calibri;font-size:11.0pt">g/tube, 1 tube,Lyophilized<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Type of Strain Stock<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt">Bacteria stock.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr> <tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Appearance<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Visual inspection<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Clear and free of foreign particles, and the strain is clearly visible.<span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:center"><span style="color:#000000;font-family:Calibri;font-size:11.0pt">Pass<span></span></span></p> | ||
+ | </td> </tr></tbody> | ||
+ | </table> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | |||
+ | <table border="1" cellpadding="0" cellspacing="0" style="border:none;border-collapse:collapse;margin-left:0.0"> | ||
+ | <tbody><tr> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="254.39999389648438" src="http://b262.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/cI8hqU6TnQQhJWgxrwgmQv0Mwgv4T3KwluBqsv0K9KA!/b/dAYBAAAAAAAA&bo=sAD*AAAAAAADAGo! " width="175.1999969482422"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="font-family:SimSun;font-size:12.0pt"><img height="259.20001220703125" src="http://b198.photo.store.qq.com/psb?/f3519ecf-9784-4a34-bd62-7aa5babb753b/94oeXP5iFtJKrKNWD4RQCmRJXVM2sO.rQztD9R2O50w!/b/dMYAAAAAAAAA&bo=qQAEAQAAAAADAIk! " width="168.8000030517578"></span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p> | ||
+ | </td> <td style="border-bottom:solid #A3A3A3 1.0pt;border-left:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;border-top:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt" valign="top"> <p style="text-align:left"><span style="color:#000000;font-family:Calibri"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane M: DNA Marker<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 1: Plasmid digested by XbaI and PstI<span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#000000;font-family:Calibri">Lane 2: Plasmid DNA <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | <p style="text-align:left"><span style="color:#595959;font-family:Calibri;font-size:9.0pt;font-style:italic"> </span><span style="font-family:SimSun;font-size:12.0pt"><span></span></span></p> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | </td> </tr></tbody> | ||
+ | </table> | ||
+ | <p style="text-align:left"><span style="font-family:Calibri;font-size:11.0pt"> <span></span></span></p> | ||
+ | <p style="text-align:justify;text-justify:inter-ideograph"><span style="font-family:等线"> </span></p> | ||
</div> | </div> | ||
+ | </div> | ||
+ | <br/> | ||
+ | </div> | ||
+ | |||
</div> | </div> | ||
</html> | </html> |
Revision as of 03:35, 14 October 2016
Cry4Aa4+Extended FMDV 2A-pChlamy_3
Gene Name | Cry4Aa4+Extended FMDV 2A |
Cloning Vector | pChlamy_3 |
Construct Resistant | Ampicillin in DH5α |
Gene Length | 3673bp |
Cloning Strategy | XbaI and PstI |
QC Items | Method | Specifications | Results |
Target Sequence | Sequence alignment | Sequencing results are consistent to the confirmed sequence. | Pass |
Vector Sequence | Sequence alignment | 20bp flanking sequences of the vector are correct. | Pass |
Reading Frame | Sequence alignment | Frame is correct and consistent to the client's requirement. | Pass |
Fragment Size | Restriction Digests | The size of inserted fragment is right and free of any contaminated bands. | Pass |
Quality/Quantity | UV spectrophotometry | Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized | Pass |
Type of Strain Stock | Visual inspection | Bacteria stock. | Pass |
Appearance | Visual inspection | Clear and free of foreign particles, and the strain is clearly visible. | Pass |
|
| Lane M: DNA Marker
Lane 1: Plasmid digested by XbaI and PstI
Lane 2: Plasmid DNA |
Cry4Aa4+Extended FMDV 2A-pChlamy_3
Gene Name | Cry10Aa4+Extended FMDV 2A |
Cloning Vector | pChlamy_3 |
Construct Resistant | Ampicillin in Top10 |
Gene Length | 2158bp |
Cloning Strategy | XbaI and PstI |
QC Items | Method | Specifications | Results |
Target Sequence | Sequence alignment | Sequencing results are consistent to the confirmed sequence. | Pass |
Vector Sequence | Sequence alignment | 20bp flanking sequences of the vector are correct. | Pass |
Reading Frame | Sequence alignment | Frame is correct and consistent to the client's requirement. | Pass |
Fragment Size | Restriction Digests | The size of inserted fragment is right and free of any contaminated bands. | Pass |
Quality/Quantity | UV spectrophotometry | Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized | Pass |
Type of Strain Stock | Visual inspection | Bacteria stock. | Pass |
Appearance | Visual inspection | Clear and free of foreign particles, and the strain is clearly visible. | Pass |
|
| Lane M: DNA Marker
Lane 1: Plasmid digested by XbaI and PstI
Lane 2: Plasmid DNA |
Cry11Aa4+ Extended FMDV 2A-pChlamy_3
Gene Name | Cry11Aa4+ Extended FMDV 2A |
Cloning Vector | pChlamy_3 |
Construct Resistant | Ampicillin in JM108 |
Gene Length | 2071bp |
Cloning Strategy | XbaI and PstI |
QC Items | Method | Specifications | Results |
Target Sequence | Sequence alignment | Sequencing results are consistent to the confirmed sequence. | Pass |
Vector Sequence | Sequence alignment | 20bp flanking sequences of the vector are correct. | Pass |
Reading Frame | Sequence alignment | Frame is correct and consistent to the client's requirement. | Pass |
Fragment Size | Restriction Digests | The size of inserted fragment is right and free of any contaminated bands. | Pass |
Quality/Quantity | UV spectrophotometry | Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized | Pass |
Type of Strain Stock | Visual inspection | Bacteria stock. | Pass |
Appearance | Visual inspection | Clear and free of foreign particles, and the strain is clearly visible. | Pass |
|
| Lane M: DNA Marker
Lane 1: Plasmid digested by XbaI and PstI
Lane 2: Plasmid DNA
|
Cyt1-pChlamy_3
Gene Name | Cyt1 |
Cloning Vector | pChlamy_3 |
Construct Resistant | Ampicillin in JM108 |
Gene Length | 762bp |
Cloning Strategy | XbaI and PstI |
QC Items | Method | Specifications | Results |
Target Sequence | Sequence alignment | Sequencing results are consistent to the confirmed sequence. | Pass |
Vector Sequence | Sequence alignment | 20bp flanking sequences of the vector are correct. | Pass |
Reading Frame | Sequence alignment | Frame is correct and consistent to the client's requirement. | Pass |
Fragment Size | Restriction Digests | The size of inserted fragment is right and free of any contaminated bands. | Pass |
Quality/Quantity | UV spectrophotometry | Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized | Pass |
Type of Strain Stock | Visual inspection | Bacteria stock. | Pass |
Appearance | Visual inspection | Clear and free of foreign particles, and the strain is clearly visible. | Pass |
|
|
Lane M: DNA Marker
Lane 1: Plasmid digested by XbaI and PstI
Lane 2: Plasmid DNA
|
Cyt2-pChlamy_3
Gene Name | Cyt1 |
Cloning Vector | pChlamy_3 |
Construct Resistant | Ampicillin in JM108 |
Gene Length | 801bp |
Cloning Strategy | XbaI and PstI |
QC Items | Method | Specifications | Results | |||
Target Sequence | Sequence alignment | Sequencing results are consistent to the confirmed sequence. | Pass | |||
Vector Sequence | Sequence alignment | 20bp flanking sequences of the vector are correct. | Pass | |||
Reading Frame | Sequence alignment | Frame is correct and consistent to the client's requirement. | Pass | |||
Fragment Size | Restriction Digests | The size of inserted fragment is right and free of any contaminated bands. | Pass | |||
Quality/Quantity | UV spectrophotometry | Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized | Pass | |||
Type of Strain Stock | Visual inspection | Bacteria stock. | Pass | |||
Appearance | Visual inspection | Clear and free of foreign particles, and the strain is clearly visible. | Pass | |||
|
|
Lane M: DNA Marker
Lane 1: Plasmid digested by XbaI and PstI
Lane 2: Plasmid DNA
|
| |||
EGFP-pChlamy_3
Gene Name | 06-EGFP(TAA) |
Cloning Vector | pChlamy_3 |
Construct Resistant | Ampicillin in Top10 |
Gene Length | 732bp |
Cloning Strategy | XbaI and PstI |
QC Items | Method | Specifications | Results |
Target Sequence | Sequence alignment | Sequencing results are consistent to the confirmed sequence. | Pass |
Vector Sequence | Sequence alignment | 20bp flanking sequences of the vector are correct. | Pass |
Reading Frame | Sequence alignment | Frame is correct and consistent to the client's requirement. | Pass |
Fragment Size | Restriction Digests | The size of inserted fragment is right and free of any contaminated bands. | Pass |
Quality/Quantity | UV spectrophotometry | Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized | Pass |
Type of Strain Stock | Visual inspection | Bacteria stock. | Pass |
Appearance | Visual inspection | Clear and free of foreign particles, and the strain is clearly visible. | Pass |
|
|
Lane M: DNA Marker
Lane 1: Plasmid digested by XbaI and PstI
Lane 2: Plasmid DNA
|
Cry4Aa4+Extended2A+Cyt2-pChlamy3
Gene Name | Cry4Aa4+Extended2A+Cyt2 |
Cloning Vector | pChlamy_3 |
Construct Resistant | Ampicillin in Top10 |
Gene Length | 4461bp |
Cloning Strategy | XbaI and PstI |
QC Items | Method | Specifications | Results |
Target Sequence | Sequence alignment | Sequencing results are consistent to the confirmed sequence. | Pass |
Vector Sequence | Sequence alignment | 20bp flanking sequences of the vector are correct. | Pass |
Reading Frame | Sequence alignment | Frame is correct and consistent to the client's requirement. | Pass |
Fragment Size | Restriction Digests | The size of inserted fragment is right and free of any contaminated bands. | Pass |
Quality/Quantity | UV spectrophotometry | Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized | Pass |
Type of Strain Stock | Visual inspection | Bacteria stock. | Pass |
Appearance | Visual inspection | Clear and free of foreign particles, and the strain is clearly visible. | Pass |
|
|
Lane M: DNA Marker
Lane 1: Plasmid digested by XbaI and BamHI
Lane 2: Plasmid DNA
|
Cry4Aa4+Extended2A+Cyt1-pChlamy3
Gene Name | Cry4Aa4+Extended2A+Cyt1-pChlamy3 |
Cloning Vector | pChlamy_3 |
Construct Resistant | Ampicillin in JM108 |
Gene Length | 4422bp |
Cloning Strategy | XbaI and PstI |
QC Items | Method | Specifications | Results | |||
Target Sequence | Sequence alignment | Sequencing results are consistent to the confirmed sequence. | Pass | |||
Vector Sequence | Sequence alignment | 20bp flanking sequences of the vector are correct. | Pass | |||
Reading Frame | Sequence alignment | Frame is correct and consistent to the client's requirement. | Pass | |||
Fragment Size | Restriction Digests | The size of inserted fragment is right and free of any contaminated bands. | Pass | |||
Quality/Quantity | UV spectrophotometry | Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized | Pass | |||
Type of Strain Stock | Visual inspection | Bacteria stock. | Pass | |||
Appearance | Visual inspection | Clear and free of foreign particles, and the strain is clearly visible. | Pass | |||
|
|
Lane M: DNA Marker
Lane 1: Plasmid digested by BamHI and KpnI
Lane 2: Plasmid DNA
|
| |||
Cry10Aa4+Extended2A+Cyt2-pChlamy3
Gene Name | Cry10Aa4+Extended2A+Cyt2-pChlamy3 |
Cloning Vector | pChlamy_3 |
Construct Resistant | Ampicillin in DH5α |
Gene Length | 2946bp |
Cloning Strategy | XbaI and PstI |
QC Items | Method | Specifications | Results |
Target Sequence | Sequence alignment | Sequencing results are consistent to the confirmed sequence. | Pass |
Vector Sequence | Sequence alignment | 20bp flanking sequences of the vector are correct. | Pass |
Reading Frame | Sequence alignment | Frame is correct and consistent to the client's requirement. | Pass |
Fragment Size | Restriction Digests | The size of inserted fragment is right and free of any contaminated bands. | Pass |
Quality/Quantity | UV spectrophotometry | Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized | Pass |
Type of Strain Stock | Visual inspection | Bacteria stock. | Pass |
Appearance | Visual inspection | Clear and free of foreign particles, and the strain is clearly visible. | Pass |
|
|
Lane M: DNA Marker
Lane 1: Plasmid digested by XbaI and PstI
Lane 2: Plasmid DNA
|
Cry10Aa4+Extended2A+Cyt2-pChlamy3
Gene Name | Cry10Aa4+Extended2A+Cyt2-pChlamy3 |
Cloning Vector | pChlamy_3 |
Construct Resistant | Ampicillin in DH5α |
Gene Length | 2946bp |
Cloning Strategy | XbaI and PstI |
QC Items | Method | Specifications | Results |
Target Sequence | Sequence alignment | Sequencing results are consistent to the confirmed sequence. | Pass |
Vector Sequence | Sequence alignment | 20bp flanking sequences of the vector are correct. | Pass |
Reading Frame | Sequence alignment | Frame is correct and consistent to the client's requirement. | Pass |
Fragment Size | Restriction Digests | The size of inserted fragment is right and free of any contaminated bands. | Pass |
Quality/Quantity | UV spectrophotometry | Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized | Pass |
Type of Strain Stock | Visual inspection | Bacteria stock. | Pass |
Appearance | Visual inspection | Clear and free of foreign particles, and the strain is clearly visible. | Pass |
|
|
Lane M: DNA Marker
Lane 1: Plasmid digested by XbaI and PstI
Lane 2: Plasmid DNA
|
Cry10Aa4+Extended2A+Cyt1-pChlamy3
Gene Name | Cry10Aa4+Extended2A+Cyt1-pChlamy3 |
Cloning Vector | pChlamy_3 |
Construct Resistant | Ampicillin in DH5α |
Gene Length | 2907bp |
Cloning Strategy | XbaI and PstI |
QC Items | Method | Specifications | Results | |||
Target Sequence | Sequence alignment | Sequencing results are consistent to the confirmed sequence. | Pass | |||
Vector Sequence | Sequence alignment | 20bp flanking sequences of the vector are correct. | Pass | |||
Reading Frame | Sequence alignment | Frame is correct and consistent to the client's requirement. | Pass | |||
Fragment Size | Restriction Digests | The size of inserted fragment is right and free of any contaminated bands. | Pass | |||
Quality/Quantity | UV spectrophotometry | Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized | Pass | |||
Type of Strain Stock | Visual inspection | Bacteria stock. | Pass | |||
Appearance | Visual inspection | Clear and free of foreign particles, and the strain is clearly visible. | Pass | |||
|
|
Lane M: DNA Marker
Lane 1: Plasmid digested by XbaI and PstI
Lane 2: Plasmid DNA
|
| |||
Cry11Aa4+Extended2A+Cyt2-pChlamy3
Gene Name | Cry11Aa4+Extended2A+Cyt2-pChlamy3 |
Cloning Vector | pChlamy_3 |
Construct Resistant | Ampicillin in Top10 |
Gene Length | 2859bp |
Cloning Strategy | XbaI and PstI |
QC Items | Method | Specifications | Results |
Target Sequence | Sequence alignment | Sequencing results are consistent to the confirmed sequence. | Pass |
Vector Sequence | Sequence alignment | 20bp flanking sequences of the vector are correct. | Pass |
Reading Frame | Sequence alignment | Frame is correct and consistent to the client's requirement. | Pass |
Fragment Size | Restriction Digests | The size of inserted fragment is right and free of any contaminated bands. | Pass |
Quality/Quantity | UV spectrophotometry | Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized | Pass |
Type of Strain Stock | Visual inspection | Bacteria stock. | Pass |
Appearance | Visual inspection | Clear and free of foreign particles, and the strain is clearly visible. | Pass |
|
|
Lane M: DNA Marker
Lane 1: Plasmid digested by XbaI and PstI
Lane 2: Plasmid DNA
|
Cry11Aa4+Extended2A+Cyt1-pChlamy3
Gene Name | Cry11Aa4+Extended2A+Cyt1-pChlamy3 |
Cloning Vector | pChlamy_3 |
Construct Resistant | Ampicillin in JM108 |
Gene Length | 2820bp |
Cloning Strategy | XbaI and PstI |
QC Items | Method | Specifications | Results |
Target Sequence | Sequence alignment | Sequencing results are consistent to the confirmed sequence. | Pass |
Vector Sequence | Sequence alignment | 20bp flanking sequences of the vector are correct. | Pass |
Reading Frame | Sequence alignment | Frame is correct and consistent to the client's requirement. | Pass |
Fragment Size | Restriction Digests | The size of inserted fragment is right and free of any contaminated bands. | Pass |
Quality/Quantity | UV spectrophotometry | Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized | Pass |
Type of Strain Stock | Visual inspection | Bacteria stock. | Pass |
Appearance | Visual inspection | Clear and free of foreign particles, and the strain is clearly visible. | Pass |
|
|
Lane M: DNA Marker
Lane 1: Plasmid digested by XbaI and PstI
Lane 2: Plasmid DNA
|
Cry11Aa4+Extended2A+EGFP-pChlamy3
Gene Name | Cry11Aa4+Extended2A+EGFP-pChlamy3 |
Cloning Vector | pChlamy_3 |
Construct Resistant | Ampicillin in JM108 |
Gene Length | 2790bp |
Cloning Strategy | XbaI and PstI |
QC Items | Method | Specifications | Results |
Target Sequence | Sequence alignment | Sequencing results are consistent to the confirmed sequence. | Pass |
Vector Sequence | Sequence alignment | 20bp flanking sequences of the vector are correct. | Pass |
Reading Frame | Sequence alignment | Frame is correct and consistent to the client's requirement. | Pass |
Fragment Size | Restriction Digests | The size of inserted fragment is right and free of any contaminated bands. | Pass |
Quality/Quantity | UV spectrophotometry | Miniprep: OD260/280=1.7~2.0 2µg/tube, 1 tube,Lyophilized | Pass |
Type of Strain Stock | Visual inspection | Bacteria stock. | Pass |
Appearance | Visual inspection | Clear and free of foreign particles, and the strain is clearly visible. | Pass |
|
|
Lane M: DNA Marker
Lane 1: Plasmid digested by XbaI and PstI
Lane 2: Plasmid DNA
|