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− | < | + | <strong>BBa_K2032000</strong> |
<p><a href="http://parts.igem.org/Part:BBa_K2032000">sMMO</a></p> | <p><a href="http://parts.igem.org/Part:BBa_K2032000">sMMO</a></p> | ||
<p>Contains the subunits necessary to assembly the soluble methane monooxygenase (sMMO) enzyme complex. Contains MMO subunits B, C, D, X, Y, and Z, each preceded by Anderson rbs B0032, and all under lactose of IPTG inducable control. The sMMO complex oxidizes methane into methanol</p> | <p>Contains the subunits necessary to assembly the soluble methane monooxygenase (sMMO) enzyme complex. Contains MMO subunits B, C, D, X, Y, and Z, each preceded by Anderson rbs B0032, and all under lactose of IPTG inducable control. The sMMO complex oxidizes methane into methanol</p> | ||
</div> | </div> | ||
<div class="longText"> | <div class="longText"> | ||
− | < | + | <strong>BBa_K2032001</strong> |
<p><a href="http://parts.igem.org/Part:BBa_K2032001">Fructose Pathway</a></p> | <p><a href="http://parts.igem.org/Part:BBa_K2032001">Fructose Pathway</a></p> | ||
<p>Contains genes MDH, HPS, and PHI. Methanol Dehydrogenase II converts methanol into formate. HPS and PHI turn it into metabolites that feed into the glycolysis pathway.</p> | <p>Contains genes MDH, HPS, and PHI. Methanol Dehydrogenase II converts methanol into formate. HPS and PHI turn it into metabolites that feed into the glycolysis pathway.</p> | ||
</div> | </div> | ||
<div class="longText"> | <div class="longText"> | ||
− | < | + | <strong>Formate Pathway</strong> |
<p><a href="http://parts.igem.org/Part:BBa_K2032002">Formate Pathway</a></p> | <p><a href="http://parts.igem.org/Part:BBa_K2032002">Formate Pathway</a></p> | ||
<p>Contains stop codons after parts FDH and FALDH! Converts methanol into carbon dioxide, generating energy in the form of NADPH in the process.</p> | <p>Contains stop codons after parts FDH and FALDH! Converts methanol into carbon dioxide, generating energy in the form of NADPH in the process.</p> | ||
</div> | </div> | ||
<div class="longText"> | <div class="longText"> | ||
− | < | + | <strong>BBa_K2032003</strong> |
<p><a href="http://parts.igem.org/Part:BBa_K2032003">Codon optimized MDH2 with Lac/pL promoter</a></p> | <p><a href="http://parts.igem.org/Part:BBa_K2032003">Codon optimized MDH2 with Lac/pL promoter</a></p> | ||
<p>Codon optimized MDH2 with lac + pL promoter</p> | <p>Codon optimized MDH2 with lac + pL promoter</p> | ||
</div> | </div> | ||
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− | < | + | <strong>BBa_K2032004</strong> |
<p><a href="http://parts.igem.org/Part:BBa_K2032004">GroESL</a></p> | <p><a href="http://parts.igem.org/Part:BBa_K2032004">GroESL</a></p> | ||
<p>Chaperone complex</p> | <p>Chaperone complex</p> | ||
</div> | </div> | ||
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− | < | + | <strong>BBa_K2032005</strong> |
<p><a href="http://parts.igem.org/Part:BBa_K2032005">GroESL Composite</a></p> | <p><a href="http://parts.igem.org/Part:BBa_K2032005">GroESL Composite</a></p> | ||
<p>Contains promoter, rbs, and terminator along with GroESL coding region</p> | <p>Contains promoter, rbs, and terminator along with GroESL coding region</p> | ||
</div> | </div> | ||
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− | < | + | <strong>BBa_K2032006</strong> |
<p><a href="http://parts.igem.org/Part:BBa_K2032006">Fructose with RFP</a></p> | <p><a href="http://parts.igem.org/Part:BBa_K2032006">Fructose with RFP</a></p> | ||
<p>Fructose construct with RFP reporter</p> | <p>Fructose construct with RFP reporter</p> | ||
</div> | </div> | ||
<div class="longText"> | <div class="longText"> | ||
− | < | + | <strong>BBa_K2032007</strong> |
<p><a href="http://parts.igem.org/Part:BBa_K2032007">Formate with RFP</a></p> | <p><a href="http://parts.igem.org/Part:BBa_K2032007">Formate with RFP</a></p> | ||
<p>Formate construct with RFP reporter</p> | <p>Formate construct with RFP reporter</p> | ||
</div> | </div> | ||
<div class="longText"> | <div class="longText"> | ||
− | < | + | <strong>BBa_K2032008</strong> |
<p><a href="http://parts.igem.org/Part:BBa_K2032008">sMMO and GFP</a></p> | <p><a href="http://parts.igem.org/Part:BBa_K2032008">sMMO and GFP</a></p> | ||
<p>sMMO and GFP for coculture test with fructose/formate and RFP</p> | <p>sMMO and GFP for coculture test with fructose/formate and RFP</p> |
Revision as of 02:44, 13 October 2016
</div> </div>
Contains the subunits necessary to assembly the soluble methane monooxygenase (sMMO) enzyme complex. Contains MMO subunits B, C, D, X, Y, and Z, each preceded by Anderson rbs B0032, and all under lactose of IPTG inducable control. The sMMO complex oxidizes methane into methanol
Contains genes MDH, HPS, and PHI. Methanol Dehydrogenase II converts methanol into formate. HPS and PHI turn it into metabolites that feed into the glycolysis pathway.
Contains stop codons after parts FDH and FALDH! Converts methanol into carbon dioxide, generating energy in the form of NADPH in the process.
Codon optimized MDH2 with Lac/pL promoter
Codon optimized MDH2 with lac + pL promoter
Chaperone complex
Contains promoter, rbs, and terminator along with GroESL coding region
Fructose construct with RFP reporter
Formate construct with RFP reporter
sMMO and GFP for coculture test with fructose/formate and RFP