Difference between revisions of "Team:NJU-China/Attributions"

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    <h1>Targeting Part</h1>
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                <div class="nav-wrapper"><a class="page-title">Attributions</a></div>
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    <p>none</p>
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            </div>
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    <h1>Silencing Part</h1>
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        <div class="container">
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    <h2>in vitro</h2>
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            <a href="#" data-activates="nav-mobile" class="button-collapse top-nav full hide-on-large-only"> <i class="material-icons">menu</i> </a>
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    <h3>1.examine the function of one basic part: KRAS siRNA</h3>
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        </div>
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    <h4>1)western blotting</h4>
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        <ul id="nav-mobile" class="side-nav fixed">
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    <p>Examine the function of KRAS siRNA with transfection into A549 cells using Lipo2000</p>
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            <li class="logo">
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    <h4>2)Evaluate the expression level of KRAS mRNA after transfected KRAS siRNA using Lipo 2000</h4>
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                <img class="background responsive-img" src="https://static.igem.org/mediawiki/2016/c/c8/NJU_China_2016_iGEM_logo.png" alt="NJU-China LOGO"> </li>
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            <li class="bold"><a href="https://2016.igem.org/Team:NJU-China/Background" class="waves-effect waves-teal">Background</a></li>
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    <h3>2.transfect KRAS siRNA and iRGD plasmid into HEK293 cells and collect exosomes</h3>
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            <li class="bold no-padding">
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    <br>
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                <ul class="collapsible collapsible-accordion">
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    <h3>3.evaluate the effect of KRAS siRNA encapsulated by iRGD-modified exosomes on A549 cells</h3>
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                    <li class="bold"> <a class="collapsible-header waves-effect waves-teal">Project Design</a>
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    <h4>1)western blotting</h4>
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                        <div class="collapsible-body">
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    <h4>2)qPCR</h4>
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                            <ul>
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    <br>
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                                <li><a href="https://2016.igem.org/Team:NJU-China/Project_Design#RNAi_Module">RNAi Module</a></li>
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    <h3>4.cell proliferation assay</h3>
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                                <li><a href="https://2016.igem.org/Team:NJU-China/Project_Design#Target_Module">Target Module</a></li>
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    <p> To ascertain that KRAS siRNA loaded into exosomes can suppress cancer cells Proliferation for further support</p>
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                                <li><a href="https://2016.igem.org/Team:NJU-China/Project_Design#Assembly">Assembly</a></li>
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    <h3>5.transmission electron microscope assay</h3>
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                            </ul>
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    <p> Observe the morphology of exosomes loaded with KRAS siRNA and determine density</p>
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                        </div>
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                    </li>
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    <h3>6.Endotoxin detecting</h3>
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                </ul>
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            </li>
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            <li class="bold"><a href="https://2016.igem.org/Team:NJU-China/Modeling" class="waves-effect waves-teal">Modeling</a></li>
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     <h2>in vivo</h2>
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            <li class="bold no-padding">
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    <h3>1.establish the mouse model</h3>
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                <ul class="collapsible collapsible-accordion">
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    <p>Injecte mice subcutaneously with A549-LUC cells</p>
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                    <li class="bold"> <a class="collapsible-header waves-effect waves-teal">Results</a>
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    <br>
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                        <div class="collapsible-body">
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    <h3>2.treatment</h3>
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                            <ul>
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    <p>randomly assign the mice into 2 groups and treat with different injections: PBS and iRGD-modified exosomes loaded with KRAS siRNA</p>
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                                <li><a href="https://2016.igem.org/Team:NJU-China/Results#in_vitro">in vitro</a></li>
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     <br>
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                                <li><a href="https://2016.igem.org/Team:NJU-China/Results#in_vivo">in vivo</a></li>
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     <h3>3.collect data</h3>
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                                <li><a href="https://2016.igem.org/Team:NJU-China/Results#Conclusions">Conclusions</a></li>
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    <p>Kill the mice and harvest tumors, compare the weight and volumes.</p>
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                            </ul>
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    <br>
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                        </div>
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     <h3>4.western blotting</h3>
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                    </li>
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     <p> extract protein from tumor issues and evaluate the K-ras protein expression Changes</p>
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                </ul>
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     <br>
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            </li>
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     <h3>5.qPCR</h3>
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            <li class="bold"><a href="https://2016.igem.org/Team:NJU-China/Human_Practices" class="waves-effect waves-teal">Human Practices</a></li>
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     <p>extract RNA from tumor issues and evaluate the expression level of KRAS mRNA</p>
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            <li class="bold"><a href="https://2016.igem.org/Team:NJU-China/Team" class="waves-effect waves-teal">Team</a></li>
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            <li class="bold"><a href="https://2016.igem.org/Team:NJU-China/Attributions" class="waves-effect waves-teal">Attributions</a></li>
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            <li class="bold"><a href="https://2016.igem.org/Team:NJU-China/Collaborations" class="waves-effect waves-teal">Collaborations</a></li>
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            <li class="bold no-padding">
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                <ul class="collapsible collapsible-accordion">
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                    <li class="bold"> <a class="collapsible-header waves-effect waves-teal">Notebook</a>
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                        <div class="collapsible-body">
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                            <ul>
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                                <li><a href="https://2016.igem.org/Team:NJU-China/Notebook/Calendar">Calendar</a></li>
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                                <li><a href="https://2016.igem.org/Team:NJU-China/Notebook/Methods">Methods</a></li>
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                                <li><a href="https://2016.igem.org/Team:NJU-China/Notebook/Protocol">Protocol</a></li>
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                            </ul>
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                <p>Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz. Abcdefg</p>
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                <p>Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz. Abcdefg hijklmnop qrs tuv wx yz.</p>
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                    <h5 class="white-text">Acknowledgement</h5>
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                    <p class="grey-text text-lighten-4">We would like to express our most sincere gratitude to every person and institute that helped us during this whole project.</p>
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                    <h5 class="white-text">Links</h5>
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Revision as of 15:17, 16 October 2016

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Acknowledgement

We would like to express our most sincere gratitude to every person and institute that helped us during this whole project.

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2016 iGEM NJU-China