Difference between revisions of "Team:OUC-China/Collaborations"

CONTENT

Upholding the principle of cooperation and sharing, We have collaborated with several iGEM teams this year:

1.How we help others

Sincere assistance to the software of HFUT-China 2016 and help from them
Experimental help to the high school team named CIEI-BJ
Collaboration on human practice surveys with Munich United 2016
Participation in newsletter of XMU-China 2016

2．How others help us

Scientific measurement from JNFLS 2016
Useful suggestions from Peking 2016

1. How we help others

Sincere assistance to the software of HFUT-China 2016 and help from them

This year, HFUT-China designed a software named BioDesigner, the purpose of which is to recommend biobricks to those who are exhausted by the countless literature. From the website, it is convenient for users to acquire the relationship between genes or parts and related diseases through visualization. We were interested in their project and collaborated with them from various ways.

Once after their preliminary design, we were the initial users to test how the software worked, using our sequences and some detailed data on probation. Based on the results, we firstly concluded a meticulous report to feedback not only for parameter choice but also the literature analysis. Then we also provided suggestions of expanding the databases to a wealth of non-standard parts or collecting experimental datas of more parts for more optimal protocol. Receiving our feedback, they thought highly of our suggestions and debugged the system again, which has perfected their software to a broad use.

What’ more, for better user interface design, we offered sincere opinions about the software we have employed in daily researches, such as Primer Premier 5, SnapGene, MEGA6, etc. Our feedback advanced their optimization about user design and therefore the software becomed more user-friendly.

After optimization of their software, they helped us characterize an essential component RNase E, with high cutting efficiency, which has greatly influenced our experiment design. In addition, they also had sent us an important reagent LUDOX.

Figure 1. The software of HFUT-China 2016

Experiment help to a high-school team named CIEI-BJ

This summer CIEI-BJ aimed to produce one of the key enzymes copalyl diphosphate synthase 1 (SmCPS1) which initiate biosynthesis of tanshinone, a famous Chinese medicinal herb, by building up a gene circuit in plasmid of Escherichia coli. However, for a brand new high school team, they are lack of parts and devices to practice, which is essential for their plasmid constructions. On the contrary, we have took part in iGEM competition with our own independent laboratory for at least six years, perfectly storing a wealth of parts and devices and inheriting proficient technology from our last iGEM team members. With great wholeheartedness, we took an initiative to send them some parts (J04450 with backbone pSBA2 and the splicing vector J23106 and I13504 with pSB1C3)for daily practice and pre-experiments.

Figure 2. Team members of CEDJ-BI

Collaboration on human practice surveys with Munich United 2016

Munich United 2016 released a survey about the living conditions of iGEM teams with various questions like the combination between iGEM team and industrial application, and so on. In order to cooperate with them for better improvements, we finished their survey conscientiously. In return, we got the badge due to our contribution.

Participate in newsletter of XMU-China 2016

This year, XMU-China 2016 issued newsletter of iGEM which would help iGEMers from all over the world to share information about their projects and the latest news about synthetic biology in general. We were honored to collaborate with XMU 2016 to publish the survey of our project.

Figure 3. Newsletter made by XMU-China 2016

2. How others help us

Scientific measurement from JNFLS 2016

In our project, the reference circuit is important for our data analysis and then more accurate datas are needed for quantitative measurement of our regular element. JNFLS helped us to measure the fluorescence intensity of GFP and mcherry in the reference circuit using flow cytometry and plate reader. With their assistance, we have assured that plate reader is more suitable for measurement of fluorescence intensity of GFP and mCherry. What’ more, in accordance with our protocol, they suggested us to measure the fluorescence when the OD is up to 0.45.

Figure 4. The fluorescence of GFP measured by flow cytometry

All the data is from the reference circuit. OUC-0 is without induction, OUC-1,OUC-2,OUC-3 is three biological repetition.GFP means the positive control.CKN is the negative control.

Figure 5. The fluorescence of GFP measured by plate reader

Figure 6. Team members of JNFLS 2016

Useful suggestions from Peking 2016

We went to Beijing in May this year to communicate with Peking iGEMers. We got lots of suggestion about our project design and their instructors also helped us to process the data more logically.

Figure 7. Picture with Peking iGEMers