Difference between revisions of "Team:Oxford/Chemicals"

Line 31: Line 31:
 
<p>Use: Staining DNA electrophoresis gels.</p>
 
<p>Use: Staining DNA electrophoresis gels.</p>
 
<p>Signal word Danger</p>
 
<p>Signal word Danger</p>
 +
 +
<img src="https://static.igem.org/mediawiki/2016/0/02/T--Oxford--ETHIDIUMBROMIDE.png" width="100%"/>
 +
 
<p style="text-align:center"><strong>Hazard statement(s)</strong></p>
 
<p style="text-align:center"><strong>Hazard statement(s)</strong></p>
 
<ul>
 
<ul>
Line 46: Line 49:
 
<!-- TBE -->
 
<!-- TBE -->
 
<h2>TBE Buffer pH 8.3</h2>
 
<h2>TBE Buffer pH 8.3</h2>
−
<p>Use: General buffer for DNA experiments.</p>
+
<p>Use: General buffer for DNA experiments. Made of three chemicals: Tris, borate and EDTA.</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
 +
 +
<img src="https://static.igem.org/mediawiki/2016/4/47/T--Oxford--TBE.gif" width="100%"/>
 +
 
<!-- Copper Sulphate -->
 
<!-- Copper Sulphate -->
 
<h2>Copper Sulphate Pentahydrate:</h2>
 
<h2>Copper Sulphate Pentahydrate:</h2>
−
<p>Use: Induction of copper promotors and Cu2+ chelation assays.</p>
+
<p>Use: Induction of copper promoters and Cu2+ chelation assays.</p>
 
<p>Signal word: Warning</p>
 
<p>Signal word: Warning</p>
 +
 +
<img src="https://static.igem.org/mediawiki/2016/b/b6/T--Oxford--CUSO4H2O.png" width="100%"/>
 +
 
<p style="text-align:center"><strong>Hazard statement(s)</strong></p>
 
<p style="text-align:center"><strong>Hazard statement(s)</strong></p>
 
<ul>
 
<ul>
Line 69: Line 78:
 
<p>Use: DNA electrophoresis gel matrix</p>
 
<p>Use: DNA electrophoresis gel matrix</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
 +
 +
<img src="https://static.igem.org/mediawiki/2016/a/af/T--Oxford--Arabinose.png" width="100%"/>
 +
 
<h2>L(+)-Arabinose</h2>
 
<h2>L(+)-Arabinose</h2>
 
<p>Use: Induction of pBAD expression</p>
 
<p>Use: Induction of pBAD expression</p>
Line 80: Line 92:
 
<p>Use: Reducing agent in Cu+/Cu2+ detection assay</p>
 
<p>Use: Reducing agent in Cu+/Cu2+ detection assay</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
 +
 +
<img src="https://static.igem.org/mediawiki/2016/4/47/T--Oxford--Ascorbate.png" width="100%"/>
 +
 
<!-- Glutathione -->
 
<!-- Glutathione -->
 
<h2>L-Glutathione</h2>
 
<h2>L-Glutathione</h2>
 
<p>Use: Reducing agent in Cu+/Cu2+ detection assay.</p>
 
<p>Use: Reducing agent in Cu+/Cu2+ detection assay.</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
 +
 +
<img src="https://static.igem.org/mediawiki/2016/d/dd/T--Oxford--Glutathione.png" width="100%"/>
 +
 
<!-- Chloramphenicol -->
 
<!-- Chloramphenicol -->
 
<h2>Chloramphenicol</h2>
 
<h2>Chloramphenicol</h2>
 
<p>Use: Antibiotic and positive selection marker for all plasmids except pBAD</p>
 
<p>Use: Antibiotic and positive selection marker for all plasmids except pBAD</p>
 
<p>Signal word: Danger</p>
 
<p>Signal word: Danger</p>
 +
 +
<img src="https://static.igem.org/mediawiki/2016/c/cd/T--Oxford--Chloramphenicol.png" width="100%"/>
 +
 +
 
<p style="text-align:center"><strong>Hazard statement(s)</strong></p>
 
<p style="text-align:center"><strong>Hazard statement(s)</strong></p>
 
<ul>
 
<ul>
Line 101: Line 123:
 
<p>Use: Antibiotic and positive selection marker for pBAD.</p>
 
<p>Use: Antibiotic and positive selection marker for pBAD.</p>
 
<p>Signal word: Danger</p>
 
<p>Signal word: Danger</p>
 +
 +
<img src="https://static.igem.org/mediawiki/2016/8/8f/T--Oxford--Ampicillin.png" width="100%"/>
 +
 
<p style="text-align:center"><strong>Hazard statement(s)</strong></p>
 
<p style="text-align:center"><strong>Hazard statement(s)</strong></p>
 
<ul>
 
<ul>
Line 120: Line 145:
 
<p>Use: General buffer for protein and copper assays</p>
 
<p>Use: General buffer for protein and copper assays</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
 +
 +
<img src="https://static.igem.org/mediawiki/2016/6/62/T--Oxford--TRIS.png" width="100%"/>
 +
 
<!-- Glycerol -->
 
<!-- Glycerol -->
 
<h2>Glycerol</h2>
 
<h2>Glycerol</h2>
 
<p>Use: Component of protein buffer for protein stability at low temperatures. Used in protein purification lysis and elution buffers. Also, used in SDS-PAGE run buffer</p>
 
<p>Use: Component of protein buffer for protein stability at low temperatures. Used in protein purification lysis and elution buffers. Also, used in SDS-PAGE run buffer</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
 +
 +
<img src="https://static.igem.org/mediawiki/2016/f/f6/T--Oxford--Glycerol.png" width="100%"/>
 +
 +
 
<!-- DTT -->
 
<!-- DTT -->
 
<h2>DL-Dithiothreitol</h2>
 
<h2>DL-Dithiothreitol</h2>
 
<p>Use: Reducing agent in Cu+/Cu2+ detection assay and protein purification</p>
 
<p>Use: Reducing agent in Cu+/Cu2+ detection assay and protein purification</p>
 
<p>Signal word: Warning</p>
 
<p>Signal word: Warning</p>
 +
 +
<img src="https://static.igem.org/mediawiki/2016/e/ee/T--Oxford--DTT.png" width="100%"/>
 +
 
<p style="text-align:center"><strong>Hazard statement(s)</strong></p>
 
<p style="text-align:center"><strong>Hazard statement(s)</strong></p>
 
<ul>
 
<ul>
Line 144: Line 179:
 
<p>Use: Elution of His-tagged target proteins from nickel bead column during protein purification</p>
 
<p>Use: Elution of His-tagged target proteins from nickel bead column during protein purification</p>
 
<p>Signal word: Danger</p>
 
<p>Signal word: Danger</p>
 +
 +
<img src="https://static.igem.org/mediawiki/2016/0/01/T--Oxford--Imidiazole.png" width="100%"/>
 +
 +
 
<p style="text-align:center"><strong>Hazard statement(s)</strong></p>
 
<p style="text-align:center"><strong>Hazard statement(s)</strong></p>
 
<ul>
 
<ul>
Line 161: Line 200:
 
<p>Use: Selection of His-tagged target proteins during protein purification</p>
 
<p>Use: Selection of His-tagged target proteins during protein purification</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
 +
 +
<img src="https://static.igem.org/mediawiki/2016/b/b9/T--Oxford--NiBEADS.gif" width="100%"/>
 +
 
<!-- Sodium Alginate -->
 
<!-- Sodium Alginate -->
 
<h2>Sodium Alginate</h2>
 
<h2>Sodium Alginate</h2>
 
<p>Use: Major component of bacterial delivery beads</p>
 
<p>Use: Major component of bacterial delivery beads</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
 +
 +
<img src="https://static.igem.org/mediawiki/2016/8/8a/T--Oxford--ALGINATE.png" width="100%"/>
 +
 
<!-- Crystal Violet -->
 
<!-- Crystal Violet -->
 
<h2>Crystal Violet</h2>
 
<h2>Crystal Violet</h2>
 
<p>Use: Marker for bead quality assay</p>
 
<p>Use: Marker for bead quality assay</p>
 
<p>Signal word: Danger</p>
 
<p>Signal word: Danger</p>
 +
 +
<img src="https://static.igem.org/mediawiki/2016/f/f6/T--Oxford--Crystal_Violet.png" width="100%"/>
 +
 +
 
<p style="text-align:center"><strong>Hazard statement(s)</strong></p>
 
<p style="text-align:center"><strong>Hazard statement(s)</strong></p>
 
<ul>
 
<ul>
Line 189: Line 238:
 
<p>Use: Media for general bacterial growth</p>
 
<p>Use: Media for general bacterial growth</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
 +
 +
<img src="https://static.igem.org/mediawiki/2016/a/ab/T--Oxford--LB.png"/>
 +
 
<!-- Phosphate buffered saline -->
 
<!-- Phosphate buffered saline -->
 
<h2>Phosphate Buffered Saline pH 7.4</h2>
 
<h2>Phosphate Buffered Saline pH 7.4</h2>
 
<p>Use: Dilution buffer for flow cytometry experiments</p>
 
<p>Use: Dilution buffer for flow cytometry experiments</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
 +
 +
<img src="https://static.igem.org/mediawiki/2016/c/c1/T--Oxford--PBS.gif"/>
 +
 
<!-- SDS -->
 
<!-- SDS -->
 
<h2>Sodium Dodecyl Sulphate</h2>
 
<h2>Sodium Dodecyl Sulphate</h2>
 
<p>Use: Detergent in protein identification gels</p>
 
<p>Use: Detergent in protein identification gels</p>
 
<p>Signal word: Danger</p>
 
<p>Signal word: Danger</p>
 +
 +
<img src="https://static.igem.org/mediawiki/2016/1/15/T--Oxford--SDS.jpg"/>
 +
 
<p style="text-align:center"><strong>Hazard statement(s)</strong></p>
 
<p style="text-align:center"><strong>Hazard statement(s)</strong></p>
 
<ul>
 
<ul>
Line 217: Line 275:
 
<!-- Ethanol -->
 
<!-- Ethanol -->
 
<h2>Ethanol 100%</h2>
 
<h2>Ethanol 100%</h2>
−
<p>Use: Solvent for chloramphenicol</p>
+
<p>Use: solvent for chloramphenicol</p>
 
<p>Signal word: Danger</p>
 
<p>Signal word: Danger</p>
 +
 +
<img src="https://static.igem.org/mediawiki/2016/b/b2/T--Oxford--Ethanol.png"/>
 +
 
<p style="text-align:center"><strong> Hazard statement(s)</strong></p>
 
<p style="text-align:center"><strong> Hazard statement(s)</strong></p>
 
<ul>
 
<ul>
Line 235: Line 296:
 
<p>Use: First soaking buffer in creating competent E. coli cells</p>
 
<p>Use: First soaking buffer in creating competent E. coli cells</p>
 
<p>Signal word: Danger</p>
 
<p>Signal word: Danger</p>
 +
 +
<img src="https://static.igem.org/mediawiki/2016/f/fa/T--Oxford--MnCl.png"/>
 +
 
<p style="text-align:center"><strong>Hazard statement(s)</strong></p>
 
<p style="text-align:center"><strong>Hazard statement(s)</strong></p>
 
<ul>
 
<ul>
Line 275: Line 339:
 
<p>Use: Stabilizer and marker for DNA gel electrophoresis</p>
 
<p>Use: Stabilizer and marker for DNA gel electrophoresis</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
−
<!-- Protease Inhibitors -->
+
 
 +
<img src="https://static.igem.org/mediawiki/2016/8/88/T--Oxford--pURPLE_LOADING_DYE.jpg"/>
 +
 
 +
<!-- Protease Inhibitors -->
 
<h2>Roche cOmplete EDTA-free Protease Inhibitor Cocktail</h2>
 
<h2>Roche cOmplete EDTA-free Protease Inhibitor Cocktail</h2>
 
<p>Use: Prevention of protein cleavage during protein purification</p>
 
<p>Use: Prevention of protein cleavage during protein purification</p>
 
<p>Signal word Danger</p>
 
<p>Signal word Danger</p>
 +
 +
<img src="https://static.igem.org/mediawiki/2016/6/68/T--Oxford--protease_inhibitors.jpg"/>
 +
 
<p style="text-align:center"><strong> Hazard statement(s)</strong></p>
 
<p style="text-align:center"><strong> Hazard statement(s)</strong></p>
 
<ul>
 
<ul>
Line 296: Line 366:
 
<p>Use: Simulation of stomach acid conditions in bead experiments</p>
 
<p>Use: Simulation of stomach acid conditions in bead experiments</p>
 
<p>Signal word: Warning</p>
 
<p>Signal word: Warning</p>
 +
 +
<img src="https://static.igem.org/mediawiki/2016/f/f5/T--Oxford--HCL.png"/>
 +
 +
 
<p style="text-align:center"><strong> Hazard statement(s)</strong></p>
 
<p style="text-align:center"><strong> Hazard statement(s)</strong></p>
 
<ul>
 
<ul>
Line 304: Line 378:
 
<p>Use: Simulation of duodenum conditions in bead experiments</p>
 
<p>Use: Simulation of duodenum conditions in bead experiments</p>
 
<p>Signal word: Danger</p>
 
<p>Signal word: Danger</p>
 +
 +
<img src="https://static.igem.org/mediawiki/2016/0/0d/T--Oxford--NaOH.jpg"/>
 +
 +
 
<p style="text-align:center"><strong> Hazard statement(s)</strong></p>
 
<p style="text-align:center"><strong> Hazard statement(s)</strong></p>
 
<ul>
 
<ul>
Line 321: Line 399:
 
<p>Use: Simulation of duodenum conditions in bead experiments</p>
 
<p>Use: Simulation of duodenum conditions in bead experiments</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
 +
 +
<img src="https://static.igem.org/mediawiki/2016/0/0d/T--Oxford--NaOH.jpg"/>
 +
 +
 
<!-- Glacial Acetic ACid -->
 
<!-- Glacial Acetic ACid -->
 
<h2>Glacial Acetic Acid</h2>
 
<h2>Glacial Acetic Acid</h2>
 
<p>Use: Bead experiments</p>
 
<p>Use: Bead experiments</p>
 
<p>Signal word: Danger</p>
 
<p>Signal word: Danger</p>
 +
 +
<img src="https://static.igem.org/mediawiki/2016/1/16/T--Oxford--Acetic_Acid.png"/>
 +
 
<p style="text-align:center"><strong> Hazard statement(s)</strong></p>
 
<p style="text-align:center"><strong> Hazard statement(s)</strong></p>
 
<ul>
 
<ul>
Line 343: Line 428:
 
<p>Use: Forming beads from alginate.  Ca2+ causes alginate phase change</p>
 
<p>Use: Forming beads from alginate.  Ca2+ causes alginate phase change</p>
 
<p>Signal word Warning</p>
 
<p>Signal word Warning</p>
 +
 +
<img src="https://static.igem.org/mediawiki/2016/8/85/T--Oxford--CACL2.png"/>
 +
 
<p style="text-align:center"><strong> Hazard statement(s)</strong></p>
 
<p style="text-align:center"><strong> Hazard statement(s)</strong></p>
 
<ul>
 
<ul>

Revision as of 21:18, 15 October 2016

iGEM Oxford 2016 - Cure for Copper

Chemicals

Ethidium Bromide

Use: Staining DNA electrophoresis gels.

Signal word Danger

Hazard statement(s)

  • H302 Harmful if swallowed.
  • H330 Fatal if inhaled.
  • H341 Suspected of causing genetic defects.

Precautionary statement(s)

  • P260 Do not breathe dust/fume/gas/mist/vapours/spray.
  • P281 Use personal protective equipment as required.
  • P284 Wear respiratory protection.
  • P310 Immediately call a POISON CENTER or doctor/ physician.

TBE Buffer pH 8.3

Use: General buffer for DNA experiments. Made of three chemicals: Tris, borate and EDTA.

Non-Hazardous

Copper Sulphate Pentahydrate:

Use: Induction of copper promoters and Cu2+ chelation assays.

Signal word: Warning

Hazard statement(s)

  • H302 Harmful if swallowed.
  • H315 Causes skin irritation.
  • H319 Causes serious eye irritation.
  • H410 Very toxic to aquatic life with long lasting effects.

Precautionary statement(s)

  • P273 Avoid release to the environment.
  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P501 Dispose of contents/container to an approved waste disposal plant.

Agarose

Use: DNA electrophoresis gel matrix

Non-Hazardous

L(+)-Arabinose

Use: Induction of pBAD expression

Non-Hazardous

Bathocuproinedisulfonic Acid Disodium Salt

Use: Chromophore in Cu+/Cu2+ detection assay

Non-Hazardous

L(+)-Sodium Ascorbate

Use: Reducing agent in Cu+/Cu2+ detection assay

Non-Hazardous

L-Glutathione

Use: Reducing agent in Cu+/Cu2+ detection assay.

Non-Hazardous

Chloramphenicol

Use: Antibiotic and positive selection marker for all plasmids except pBAD

Signal word: Danger

Hazard statement(s)

  • H350 May cause cancer

Precautionary statement(s)

  • P201 Obtain special instructions before use.
  • P308 + P313 If exposed or concerned: Get medical advice/attention.

Ampicillin

Use: Antibiotic and positive selection marker for pBAD.

Signal word: Danger

Hazard statement(s)

  • H315 Causes skin irritation
  • H317 May cause an allergic skin reaction
  • H319 Causes serious eye irritation
  • H334 May cause allergy or asthma symptoms or breathing difficulties if inhaled
  • H335 May cause respiratory irritation

Precautionary statement(s)

  • P261 Avoid breathing dust/fume/gas/mist/vapours/spray
  • P280 Wear protective gloves
  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
  • P342 + P311 If experiencing respiratory symptoms: Call a POISON CENTER or doctor/physician

Tris pH 7.0 and 8.0

Use: General buffer for protein and copper assays

Non-Hazardous

Glycerol

Use: Component of protein buffer for protein stability at low temperatures. Used in protein purification lysis and elution buffers. Also, used in SDS-PAGE run buffer

Non-Hazardous

DL-Dithiothreitol

Use: Reducing agent in Cu+/Cu2+ detection assay and protein purification

Signal word: Warning

Hazard statement(s)

  • H302 Harmful if swallowed
  • H315 Causes skin irritation
  • H319 Causes serious eye irritation
  • H335 May cause respiratory irritation

Precautionary statement(s)

  • P261 Avoid breathing dust
  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing

Imidazole

Use: Elution of His-tagged target proteins from nickel bead column during protein purification

Signal word: Danger

Hazard statement(s)

  • H302 Harmful if swallowed
  • H314 Causes severe skin burns and eye damage
  • H360D May damage the unborn child

Precautionary statement(s)

  • P201 Obtain special instructions before use
  • P280 Wear protective gloves/protective clothing/eye protection/face protection
  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
  • P310 Immediately call a POISON CENTER or doctor/physician

Nickel Affinity Beads

Use: Selection of His-tagged target proteins during protein purification

Non-Hazardous

Sodium Alginate

Use: Major component of bacterial delivery beads

Non-Hazardous

Crystal Violet

Use: Marker for bead quality assay

Signal word: Danger

Hazard statement(s)

  • H302 Harmful if swallowed
  • H318 Causes serious eye damage
  • H350 May cause cancer
  • H410 Very toxic to aquatic life with long lasting effects

Precautionary statement(s)

  • P201 Obtain special instructions before use
  • P273 Avoid release to the environment
  • P280 Wear protective gloves/ eye protection/ face protection
  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
  • P308 + P313 If exposed or concerned: Get medical advice/attention
  • P501 Dispose of contents/container to an approved waste disposal plant

Lysogeny Broth

Use: Media for general bacterial growth

Non-Hazardous

Phosphate Buffered Saline pH 7.4

Use: Dilution buffer for flow cytometry experiments

Non-Hazardous

Sodium Dodecyl Sulphate

Use: Detergent in protein identification gels

Signal word: Danger

Hazard statement(s)

  • H228 Flammable solid
  • H302 + H332 Harmful if swallowed or if inhaled
  • H315 Causes skin irritation
  • H318 Causes serious eye damage
  • H335 May cause respiratory irritation
  • H412 Harmful to aquatic life with long lasting effects

Precautionary statement(s)

  • P210 Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking
  • P261 Avoid breathing dust/fume/gas/mist/vapours/spray
  • P280 Wear eye protection/face protection
  • P301 + P312 + P330 IF SWALLOWED: Call a POISON CENTER/doctor if you feel unwell. Rinse mouth
  • P305 + P351 + P338 + P310 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER/doctor
  • P370 + P378 In case of fire: Use dry powder or dry sand to extinguish

Ethanol 100%

Use: solvent for chloramphenicol

Signal word: Danger

Hazard statement(s)

  • H225 Highly flammable liquid and vapour
  • H319 Causes serious eye irritation

Precautionary statement(s)

  • P210 Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking
  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
  • P370 + P378 In case of fire: Use dry powder or dry sand to extinguish
  • P403 + P235 Store in a well-ventilated place. Keep cool

Transfection Buffer I (Manganese[II] Chloride)

Use: First soaking buffer in creating competent E. coli cells

Signal word: Danger

Hazard statement(s)

  • H302 Harmful if swallowed
  • H318 Causes serious eye damage
  • H373 May cause damage to organs (Brain) through prolonged or repeated exposure if inhaled
  • H411 Toxic to aquatic life with long lasting effects

Precautionary statement(s)

  • P273 Avoid release to the environment
  • P280 Wear eye protection/ face protection
  • P301 + P312 + P330 IF SWALLOWED: Call a POISON CENTER or doctor/physician if you feel unwell. Rinse mouth
  • P305 + P351 + P338 + P310 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER or doctor/physician
  • P391 Collect spillage
  • P501 Dispose of contents/container to an approved waste disposal plant

Transfection Buffer II

Use: Second soaking buffer in creating competent E. coli cells

Non-Hazardous

Chitosan

Use: Coating for bacterial delivery beads

Non-Hazardous

InstantBlue

Use: Protein stain for protein identification gels

Signal word: Warning

Hazard statement(s)

  • H290 May be corrosive to metals
  • H315 Causes skin irritation
  • H319 Causes serious eye irritation

Precautionary statement(s)

  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing

NEB Purple Loading Dye

Use: Stabilizer and marker for DNA gel electrophoresis

Non-Hazardous

Roche cOmplete EDTA-free Protease Inhibitor Cocktail

Use: Prevention of protein cleavage during protein purification

Signal word Danger

Hazard statement(s)

  • H314 Causes severe skin burns and eye damage Skin corrosion/irritation

Precautionary statement(s)

  • P260 Do not breathe dust/fume/gas/mist/vapours/spray
  • P280 Wear protective gloves/protective clothing/eye protection/face protection
  • P301 + P330 + P331 IF SWALLOWED: Rinse mouth. Do NOT induce vomiting
  • P303 + P361 + P353 IF ON SKIN (or hair): Remove/Take off Immediately all contaminated clothing. Rinse SKIN with water/shower
  • P304 + P340 + P310 IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing. Immediately call a POISON CENTER or doctor/physician
  • P305 + P351 + P338 + P310 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER or doctor/physician

Hydrochloric Acid 1M

Use: Simulation of stomach acid conditions in bead experiments

Signal word: Warning

Hazard statement(s)

  • H290 May be corrosive to metals

Sodium Hydroxide

Use: Simulation of duodenum conditions in bead experiments

Signal word: Danger

Hazard statement(s)

  • H290 May be corrosive to metals
  • H314 Causes severe skin burns and eye damage

Precautionary statement(s)

  • P260 Do not breathe dust/fume/gas/mist/vapours/spray
  • P280 Wear protective gloves/protective clothing/eye protection/face protection
  • P303 + P361 + P353 IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/shower
  • P304 + P340 + P310 IF INHALED: Remove person to fresh air and keep comfortable for breathing. Immediately call a POISON CENTER/doctor
  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing

Monobasic Sodium Phosphate

Use: Simulation of duodenum conditions in bead experiments

Non-Hazardous

Glacial Acetic Acid

Use: Bead experiments

Signal word: Danger

Hazard statement(s)

  • H226 Flammable liquid and vapour
  • H314 Causes severe skin burns and eye damage

Precautionary statement(s)

  • P210 Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking
  • P260 Do not breathe dust/fume/gas/mist/vapours/spray
  • P280 Wear protective gloves/protective clothing/eye protection/face protection
  • P303 + P361 + P353 IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/shower
  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
  • P370 + P378 In case of fire: Use dry powder or dry sand to extinguish

Calcium Chloride

Use: Forming beads from alginate. Ca2+ causes alginate phase change

Signal word Warning

Hazard statement(s)

  • H319 Causes serious eye irritation

Precautionary statement(s)

  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing