Difference between revisions of "Team:UMaryland/Part Collection"
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<div class="profiles basic"> | <div class="profiles basic"> | ||
<strong><a href="http://parts.igem.org/Part:BBa_K2032002">Formate Pathway(BBa_K2032002)</a></strong> | <strong><a href="http://parts.igem.org/Part:BBa_K2032002">Formate Pathway(BBa_K2032002)</a></strong> | ||
| + | <img src="https://static.igem.org/mediawiki/2016/a/a6/T--UMaryland--BBa_K2032002.png" class="plasmid" /> | ||
| + | <img src="https://static.igem.org/mediawiki/2016/9/9b/T--UMaryland--BBa_K2032002_linear.jpg" class="linear" /> | ||
<p>Contains stop codons after parts FDH and FALDH! Converts methanol into carbon dioxide, generating energy in the form of NADPH in the process.</p> | <p>Contains stop codons after parts FDH and FALDH! Converts methanol into carbon dioxide, generating energy in the form of NADPH in the process.</p> | ||
</div> | </div> | ||
<div class="profiles basic"> | <div class="profiles basic"> | ||
<strong><a href="http://parts.igem.org/Part:BBa_K2032003">Codon optimized MDH2 with Lac/pL promoter (BBa_K2032003)</strong></a> | <strong><a href="http://parts.igem.org/Part:BBa_K2032003">Codon optimized MDH2 with Lac/pL promoter (BBa_K2032003)</strong></a> | ||
| + | <img src="https://static.igem.org/mediawiki/2016/8/8c/T--UMaryland--BBa_K2032003_plasmid.jpg" class="plasmid" /> | ||
<p>Codon optimized MDH2 with lac + pL promoter</p> | <p>Codon optimized MDH2 with lac + pL promoter</p> | ||
</div> | </div> | ||
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<div class="profiles composite"> | <div class="profiles composite"> | ||
<strong><a href="http://parts.igem.org/Part:BBa_K2032000">sMMO (BBa_K2032000)</strong></a> | <strong><a href="http://parts.igem.org/Part:BBa_K2032000">sMMO (BBa_K2032000)</strong></a> | ||
| + | <img src="https://static.igem.org/mediawiki/2016/5/5b/T--UMaryland--BBa_K2032000_plasmid.jpg" class="plasmid" /> | ||
| + | <img src="https://static.igem.org/mediawiki/2016/4/4e/T--UMaryland--BBa_K2032000_linear.jpg" class="linear" /> | ||
<p>Contains the subunits necessary to assembly the soluble methane monooxygenase (sMMO) enzyme complex. Contains MMO subunits B, C, D, X, Y, and Z, each preceded by Anderson rbs B0032, and all under lactose of IPTG inducable control. The sMMO complex oxidizes methane into methanol</p> | <p>Contains the subunits necessary to assembly the soluble methane monooxygenase (sMMO) enzyme complex. Contains MMO subunits B, C, D, X, Y, and Z, each preceded by Anderson rbs B0032, and all under lactose of IPTG inducable control. The sMMO complex oxidizes methane into methanol</p> | ||
</div> | </div> | ||
<div class="profiles composite"> | <div class="profiles composite"> | ||
<strong><a href="http://parts.igem.org/Part:BBa_K2032001">Fructose Pathway (BBa_K2032001)</strong></a> | <strong><a href="http://parts.igem.org/Part:BBa_K2032001">Fructose Pathway (BBa_K2032001)</strong></a> | ||
| + | <img src="https://static.igem.org/mediawiki/2016/1/14/T--UMaryland--BBa_K2032001_plasmid.jpg" class="plasmid" /> | ||
| + | <img src="https://static.igem.org/mediawiki/2016/e/e7/T--UMaryland--BBa_K2032001_linear.jpg" class="linear" /> | ||
<p>Contains genes MDH, HPS, and PHI. Methanol Dehydrogenase II converts methanol into formate. HPS and PHI turn it into metabolites that feed into the glycolysis pathway.</p> | <p>Contains genes MDH, HPS, and PHI. Methanol Dehydrogenase II converts methanol into formate. HPS and PHI turn it into metabolites that feed into the glycolysis pathway.</p> | ||
</div> | </div> | ||
<div class="profiles composite"> | <div class="profiles composite"> | ||
<strong><a href="http://parts.igem.org/Part:BBa_K2032005">GroESL Composite (BBa_K2032005)</strong></a> | <strong><a href="http://parts.igem.org/Part:BBa_K2032005">GroESL Composite (BBa_K2032005)</strong></a> | ||
| + | <img src="https://static.igem.org/mediawiki/2016/3/30/T--UMaryland--BBa_K2032005_plasmid.jpg" class="plasmid" /> | ||
| + | <img src="https://static.igem.org/mediawiki/2016/5/59/T--UMaryland--BBa_K2032005_linear.jpg" class="linear" /> | ||
<p>Contains promoter, rbs, and terminator along with GroESL coding region | <p>Contains promoter, rbs, and terminator along with GroESL coding region | ||
</div> | </div> | ||
<div class="profiles composite"> | <div class="profiles composite"> | ||
<strong><a href="http://parts.igem.org/Part:BBa_K2032006">Fructose with RFP (BBa_K2032006)</strong></a> | <strong><a href="http://parts.igem.org/Part:BBa_K2032006">Fructose with RFP (BBa_K2032006)</strong></a> | ||
| + | <img src="https://static.igem.org/mediawiki/2016/f/f9/T--UMaryland--BBa_K2032006_plasmid.jpg" class="plasmid" /> | ||
| + | <img src="https://static.igem.org/mediawiki/2016/b/b3/T--UMaryland--BBa_K2032006_linear.jpg" class="linear" /> | ||
<p>Fructose construct with RFP reporter</p> | <p>Fructose construct with RFP reporter</p> | ||
</div> | </div> | ||
<div class="profiles composite"> | <div class="profiles composite"> | ||
<strong><a href="http://parts.igem.org/Part:BBa_K2032007">Formate with RFP (BBa_K2032007)</strong></a> | <strong><a href="http://parts.igem.org/Part:BBa_K2032007">Formate with RFP (BBa_K2032007)</strong></a> | ||
| + | <img src="https://static.igem.org/mediawiki/2016/9/90/T--UMaryland--BBa_K2032007_plasmid.jpg" class="plasmid" /> | ||
| + | <img src="https://static.igem.org/mediawiki/2016/f/fb/T--UMaryland--BBa_K2032007_linear.jpg" class="linear" /> | ||
<p>Formate construct with RFP reporter</p> | <p>Formate construct with RFP reporter</p> | ||
</div> | </div> | ||
<div class="profiles composite"> | <div class="profiles composite"> | ||
<strong><a href="http://parts.igem.org/Part:BBa_K2032008">sMMO and GFP (BBa_K2032008)</strong></a> | <strong><a href="http://parts.igem.org/Part:BBa_K2032008">sMMO and GFP (BBa_K2032008)</strong></a> | ||
| + | <img src="https://static.igem.org/mediawiki/2016/b/b3/T--UMaryland--BBa_K2032008_plasmid.jpg" class="plasmid" /> | ||
| + | <img src="https://static.igem.org/mediawiki/2016/0/06/T--UMaryland--BBa_K2032008_linear.jpg" class="plasmid" /> | ||
<p>sMMO and GFP for coculture test with fructose/formate and RFP</p> | <p>sMMO and GFP for coculture test with fructose/formate and RFP</p> | ||
</div> | </div> | ||
Revision as of 01:47, 16 October 2016
</div> </div>
Formate Pathway(BBa_K2032002)
Contains stop codons after parts FDH and FALDH! Converts methanol into carbon dioxide, generating energy in the form of NADPH in the process.
Codon optimized MDH2 with Lac/pL promoter (BBa_K2032003)
Codon optimized MDH2 with lac + pL promoter
GroESL (BBa_K2032004)
Chaperone complex
sMMO (BBa_K2032000)
Contains the subunits necessary to assembly the soluble methane monooxygenase (sMMO) enzyme complex. Contains MMO subunits B, C, D, X, Y, and Z, each preceded by Anderson rbs B0032, and all under lactose of IPTG inducable control. The sMMO complex oxidizes methane into methanol
Fructose Pathway (BBa_K2032001)
Contains genes MDH, HPS, and PHI. Methanol Dehydrogenase II converts methanol into formate. HPS and PHI turn it into metabolites that feed into the glycolysis pathway.
GroESL Composite (BBa_K2032005)
Contains promoter, rbs, and terminator along with GroESL coding region
© University of Maryland iGEM 2016
