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+ | </div> | ||
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<div class="profiles composite"> | <div class="profiles composite"> | ||
<strong><a href="http://parts.igem.org/Part:BBa_K2032000">sMMO (BBa_K2032000)</strong></a> | <strong><a href="http://parts.igem.org/Part:BBa_K2032000">sMMO (BBa_K2032000)</strong></a> | ||
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<img src="https://static.igem.org/mediawiki/2016/b/b3/T--UMaryland--BBa_K2032008_plasmid.jpg" class="plasmid" /> | <img src="https://static.igem.org/mediawiki/2016/b/b3/T--UMaryland--BBa_K2032008_plasmid.jpg" class="plasmid" /> | ||
<p>sMMO and GFP for coculture test with fructose/formate and RFP</p> | <p>sMMO and GFP for coculture test with fructose/formate and RFP</p> | ||
+ | </div> | ||
+ | <div class="profiles basic"> | ||
+ | <strong><a href="http://parts.igem.org/Part:BBa_K2032002">Formate Pathway (BBa_K2032002)</a></strong> | ||
+ | <img src="https://static.igem.org/mediawiki/2016/9/9b/T--UMaryland--BBa_K2032002_linear.jpg" class="linear" style="width: 944px; height: 323px;"/> | ||
+ | <img src="https://static.igem.org/mediawiki/2016/a/a6/T--UMaryland--BBa_K2032002.png" class="plasmid" /> | ||
+ | <p>Contains stop codons after parts FDH and FALDH! Converts methanol into carbon dioxide, generating energy in the form of NADPH in the process.</p> | ||
+ | </div> | ||
+ | <div class="profiles basic"> | ||
+ | <strong><a href="http://parts.igem.org/Part:BBa_K2032003">Codon optimized MDH2 with Lac/pL promoter (BBa_K2032003)</strong></a> | ||
+ | <img src="https://static.igem.org/mediawiki/2016/8/8c/T--UMaryland--BBa_K2032003_plasmid.jpg" class="plasmid" /> | ||
+ | <p>Codon optimized MDH2 with lac + pL promoter</p> | ||
+ | </div> | ||
+ | <div class="profiles basic"> | ||
+ | <strong><a href="http://parts.igem.org/Part:BBa_K2032004">GroESL (BBa_K2032004)</strong></a> | ||
+ | <p>Chaperone complex</p> | ||
</div> | </div> | ||
</div> | </div> |
Revision as of 02:06, 16 October 2016
</div> </div>
Contains the subunits necessary to assembly the soluble methane monooxygenase (sMMO) enzyme complex. Contains MMO subunits B, C, D, X, Y, and Z, each preceded by Anderson rbs B0032, and all under lactose of IPTG inducable control. The sMMO complex oxidizes methane into methanol
Contains genes MDH, HPS, and PHI. Methanol Dehydrogenase II converts methanol into formate. HPS and PHI turn it into metabolites that feed into the glycolysis pathway.
Contains promoter, rbs, and terminator along with GroESL coding region
Fructose construct with RFP reporter
Formate construct with RFP reporter
sMMO and GFP for coculture test with fructose/formate and RFP
Contains stop codons after parts FDH and FALDH! Converts methanol into carbon dioxide, generating energy in the form of NADPH in the process.
Codon optimized MDH2 with lac + pL promoter
Chaperone complex