Difference between revisions of "Team:Manchester/Model/GlucoseOxidaseReaction"

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Kcat and Km parameters were collected from
 
Kcat and Km parameters were collected from
<a href="http://www.brenda-enzymes.org/enzyme.php?ecno=1.1.3.4">BRENDA</a>
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<a href="http://www.brenda-enzymes.org/enzyme.php?ecno=1.1.3.4" target="_blank">BRENDA</a>
 
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Keq was calculated using the Van’t Hoff equation using the Gibbs free energy sourced from
 
Keq was calculated using the Van’t Hoff equation using the Gibbs free energy sourced from
<a href="http://www.metacyc.org/META/NEW-IMAGE?type=REACTION&object=GLUCOSE-OXIDASE-RXN">MetaCyc</a>
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<a href="http://www.metacyc.org/META/NEW-IMAGE?type=REACTION&object=GLUCOSE-OXIDASE-RXN" target="_blank">MetaCyc</a>
 
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Revision as of 17:43, 16 October 2016

Manchester iGEM 2016

Glucose Oxidase Reaction


Oxidation of glucose to GDL and Hydrogen Peroxide using Glucose Oxidase (EC 1.1.3.4)


Chemical Equation

Glucose + Oxygen → Glucono-δ-lactone + Hydrogen Peroxide (REWRITE IN MATHJAX!)


Rate Equation

PLACEHOLDER FOR RATE EQUATION IN MATHJAX

where: x = y = z =


Parameter Sourcing?

Kcat and Km parameters were collected from BRENDA

Keq was calculated using the Van’t Hoff equation using the Gibbs free energy sourced from MetaCyc

PLACEHOLDER FOR Van't Hoff equation IN MATHJAX


Parameters with uncertainty

Km

PLACEHOLDER FOR PDF GRAPH

Sigma = (IN MATHJAX)

Mu = (IN MATHJAX)

Uncertainty in Km is introduced through the range of experimental parameters sourced


Kcat

PLACEHOLDER FOR PDF GRAPH

Sigma = (IN MATHJAX)

Mu = (IN MATHJAX)

Uncertainty in Kcat is introduced through the range of experimental parameters sourced


Keq

PLACEHOLDER FOR PDF GRAPH

Sigma = (IN MATHJAX)

Mu = (IN MATHJAX)

Uncertainty in Keq is introduced using a uniform range of temperature variations