Difference between revisions of "Team:OUC-China/Protocol"
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| − | <li><a | + | <li><a href="https://2016.igem.org/Team:OUC-China/Project">Introduction</a></li> |
<li><a href="https://2016.igem.org/Team:OUC-China/Design">Design</a></li> | <li><a href="https://2016.igem.org/Team:OUC-China/Design">Design</a></li> | ||
<li><a href="https://2016.igem.org/Team:OUC-China/Proof">Proof of concept </a></li> | <li><a href="https://2016.igem.org/Team:OUC-China/Proof">Proof of concept </a></li> | ||
Revision as of 05:08, 17 October 2016
1. Elementary Operation
1) Luria-Bertani liquid and Luria-Bertani solid medium
2) Inoculation
3) Polymerase Chain Reaction
4) Gel Electrophoresis
5) Restriction Enzyme Digestion
6) Gel Extraction
7) Ligation system
8) Transformation of E.coli
9) Conservation
10) Sterilization
11) Plasmid extraction
2. Plasmid Construction
1) Gibson
2) DNA cassettes
3. Systems detection
1) RNA extraction
2) Reserve transcription
3) Quantitative Real-time PCR
4) Fluorescence measurement protocol
Cistrons Concerto
Thanks
1.Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences
2.NEW ENGLAND Biolabs
Contact us:
E-mail: oucigem@163.com
Designed and built by @ Jasmine Chen and @ Zexin Jiao
We are OUC-iGEM
