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− | <li><a href="https://2016.igem.org/Team:OUC-China/ | + | <li><a href="https://2016.igem.org/Team:OUC-China/Communicating">Communicating & Improving</a></li> |
− | <li><a href="https://2016.igem.org/Team:OUC-China/ | + | <li><a href="https://2016.igem.org/Team:OUC-China/Investigating">Investigating & Promoting</a></li> |
<li><a href="https://2016.igem.org/Team:OUC-China/Potential_application">Potential application</a></li> | <li><a href="https://2016.igem.org/Team:OUC-China/Potential_application">Potential application</a></li> | ||
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− | + | <video src="https://static.igem.org/mediawiki/2016/d/de/T--OUC-China--Video1.mp4" controls="controls" width="100%" height="400" style="margin:0 auto;"> | |
+ | your browser does not support the video tag | ||
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− | <a href=" | + | <a href="https://2016.igem.org/Team:OUC-China/Design"><img src="https://static.igem.org/mediawiki/2016/1/1d/T--OUC-China--menu-icon-2.fw.png" class="img-responsive" alt="Principle"><p class="text-center" style="margin-top:20px;">Design</p></a> |
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− | <a href=" | + | <a href="https://2016.igem.org/Team:OUC-China/Collaborations"><img src="https://static.igem.org/mediawiki/2016/8/8a/T--OUC-China--menu-icon-4.fw.png" class="img-responsive" alt="Attrbution"><p class="text-center" style="margin-top:20px;">Collaborations</p></a> |
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− | <h3>Thanks</h3> | + | <h3>Thanks:</h3> |
− | + | <p>1.Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences</p> | |
− | <p | + | <p>2.NEW ENGLAND Biolabs</p> |
− | <p | + | <p>3.Genscript</p> |
</div> | </div> | ||
<div class="col-md-5 Contact"> | <div class="col-md-5 Contact"> | ||
− | <h3>Contact us:</h3> | + | <h3>Contact us:</h3> |
− | + | ||
<p><b>E-mail</b>: oucigem@163.com</p> | <p><b>E-mail</b>: oucigem@163.com</p> | ||
<p><b>Designed and built</b> by @ Jasmine Chen and @ Zexin Jiao</p> | <p><b>Designed and built</b> by @ Jasmine Chen and @ Zexin Jiao</p> |
Revision as of 20:26, 19 October 2016
![icon](https://static.igem.org/mediawiki/2016/0/0c/T--OUC-China--ab-icon1.png)
ABSTRACT
![icon](https://static.igem.org/mediawiki/2016/d/d4/T--OUC-China--ab-icon2.png)
As is known, once forming stem-loops, the oligonucleotides will be more stable than the single-stranded ones. And mRNA with stem-loop at its 3’ or 5’ end often get a longer lifetime than the linear one owe to the stem-loop’s resistance to exonuclease. Our team tend to design a series of stem-loops each followed by the same endonuclease site and are transcribed as one polycistron. Once digested by endonuclease and seperate into several independent fragments, cistrons with different ∆G stem-loops will get different stability, thus influence the amount of expressed proteins. In this way, we can decouple the expression level of upstream and downstream genes of the same operon by simply designing different stem-loops. Futhermore, with quantitative ∆G of stem-loops, we even can achieve the ratio expression of target proteins. It is a creative regulating method and we attempt to provide a series of standard regulation parts for others.
Cistrons Concerto
Thanks:
1.Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences
2.NEW ENGLAND Biolabs
3.Genscript
Contact us:
E-mail: oucigem@163.com
Designed and built by @ Jasmine Chen and @ Zexin Jiao
We are OUC-iGEM