Difference between revisions of "Team:USP UNIFESP-Brazil/Parts"

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<p class="black">Our favourite composite part was the 5' 5' cassete for Chlamydomonas transgenic expression(<a href= http://parts.igem.org/wiki/index.php?title=Part:BBa_K2136010>BBa_K2136010</a>). We validated it through a series of measurements which show the production and secretion of mCherry protein by <em> Chlamydomonas</em>. A detailed descriptions is available in our <a href=https://2016.igem.org/Team:USP_UNIFESP-Brazil/Proof>Proof of concept</a> page.</p>
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<p class="black">Our favourite composite part was the 5' cassete for Chlamydomonas transgenic expression(<a href= http://parts.igem.org/wiki/index.php?title=Part:BBa_K2136010>BBa_K2136010</a>). We validated it through a series of measurements which show the production and secretion of mCherry protein by <em> Chlamydomonas</em>. A detailed descriptions is available in our <a href=https://2016.igem.org/Team:USP_UNIFESP-Brazil/Proof>Proof of concept</a> page.</p>
 
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Revision as of 23:06, 18 October 2016

Part Collection

Part Table

<groupparts>iGEM16 USP_UNIFESP-Brazil</groupparts>

The protein structure of spider silk is composed of two constant terminal domains and a variable middle structural domain. Our design uses the substitution of this middle domain by a protein of interest, as used by Team:UCLA in 2014. In our case, we are using enzybiotics.

Basic Parts

Our major contribution of a Basic Part for the iGEM community is a BioBrick coding for the catalytic protein domain of lysostaphin (BBa_K2136002). This is an antibiotic peptide domain which can be used by future teams in the development of projects fighting antibiotic resistant Staphylococcus or working with its population dynamics.

Composite Parts

Our favourite composite part was the 5' cassete for Chlamydomonas transgenic expression(BBa_K2136010). We validated it through a series of measurements which show the production and secretion of mCherry protein by Chlamydomonas. A detailed descriptions is available in our Proof of concept page.