Difference between revisions of "Team:KAIT Japan/Labnote"

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{{KAIT_Japan}}
 
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8/17<br>
 
8/17<br>
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8/24<br>
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3A assembly<br>
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Ligation<br>
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PCR<br>
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R0051 K592024 I14044 B0015 R0010 C0051
  
  
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10/16<br>
 
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electrophoresis to confirm<br>
 
electrophoresis to confirm<br>
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Revision as of 13:01, 19 October 2016


8/17
restriction emzyme fragmation
K592024 B0015 C0051→Eco RI & SpeI
I14144 R100 B0015 →XbaI & PstI
EcoRI XbaI PstI →Promega
SpeI→Takara

8/18
transcription
R0051 K592024 I14044 B0015 R0010
C0051(psbic3)→E・coli-DH5X

8/18
miniprep
J04450
colony PCR
K592024 I14044 B0015 R0010 C0051

8/23
restriction emzyme fragmation

8/24
3A assembly
Ligation
PCR
R0051 K592024 I14044 B0015 R0010 C0051
9/16
miniprep
restriction emzyme fragmation

9/19
miniprep

9/21
miniprep

9/22
restriction emzyme fragmation
miniprep
purify a gel

9/23
miniprep
purify a gel

9/24
restriction emzyme fragmation

9/25
purify a gel
ligation
transformation

9/26
miniprep

9/27
purify a gel
restriction emzyme fragmation

9/28
restriction emzyme fragmation

9/30
restriction emzyme fragmation
purify a gel

10/1
ligation
transformation
pre-culture
purification of antisensence

10/2
pre-culture
miniprep
electrophoresis to confirm

10/3
miniprep
PCR
pre-culture
purify a gel

10/4
restriction emzyme fragmation
purify a gel
miniprep
electrophoresis to confirm

10/5
ligation
transformation
purify a gel
restriction emzyme fragmation

10/6
KIB…miniprep
electrophoresis to confirm
purify a gel

10/7
restriction emzyme fragmation
ligation
transformation

10/8
miniprep
purify a gel
restriction emzyme fragmation

10/9
pre-culture
purify a gel

10/10
ligation
transformation

10/11
pre-culture
miniprep

10/12
purify a gel

10/13
electrophoresis to confirm

10/14
restriction emzyme fragmation

10/15
electrophoresis to confirm
restriction emzyme fragmation

10/16
electrophoresis to confirm