Difference between revisions of "Team:FAFU-CHINA/Composite Part"

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= <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:16.0pt;font-family:&quot;:,sans-serif;color:#1E4E79">Cry4Aa4+Extended2A+Cyt2-pChlamy3</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> =
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<table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse">
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<tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Gene Name</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Cry4Aa4+Extended2A+Cyt2</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Cloning Vector</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">pChlamy_3</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Construct Resistant</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Ampicillin in Top10</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Gene Length</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">4461bp</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Cloning Strategy</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">XbaI and PstI</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  </td> </tr>
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</table>
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<p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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<table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse">
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<tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">QC Items</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Method</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Specifications</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><b><span style="font-size:12pt;font-family:Calibri, sans-serif">Results</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Target Sequence</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequencing results are consistent to  the confirmed sequence.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Vector Sequence</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">20bp flanking sequences of the  vector are correct.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Reading Frame</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Sequence alignment</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Frame is correct and consistent to  the client's requirement.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Fragment Size</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Restriction Digests</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">The size of inserted fragment is  right and free of any contaminated bands.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Quality/Quantity</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">UV spectrophotometry</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Miniprep: OD260/280=1.7~2.0</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">2µg/tube, 1 tube,Lyophilized</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Type of Strain Stock</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Visual inspection</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Bacteria stock.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  </td> </tr> <tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-top:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Appearance</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Visual inspection</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">Clear and free of foreign particles,  and the strain is clearly visible.</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  </td>  <td valign="top" style="border-top:none;border-left:none;border-bottom:solid #A3A3A3 1.0pt;border-right:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="center" style="margin-top:4.8pt;margin-right:0cm;margin-bottom:6.0pt;margin-left:0cm;text-align:center"><span style="font-size:11pt;font-family:Calibri, sans-serif">Pass</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  </td> </tr>
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</table>
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<p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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<table border="0" cellspacing="0" cellpadding="0" width="823" style="width:617.25pt;border-collapse:collapse">
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<tr>  <td valign="top" style="border:solid #A3A3A3 1.0pt;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:12pt;font-family:宋体">    [[File:T--FAFU-CHINA--bs13.png | 825px | thumb | center ]]</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:12pt;font-family:宋体">    [[File:T--FAFU-CHINA--bs14.png | 825px | thumb | center ]]</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  </td>  <td valign="top" style="border:solid #A3A3A3 1.0pt;border-left:none;padding:2.0pt 3.0pt 2.0pt 3.0pt">  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane M: DNA Marker</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane 1: Plasmid digested by XbaI and  BamHI</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
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  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:10pt;font-family:Calibri, sans-serif">Lane 2: Plasmid DNA</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><i><span style="font-size:9.0pt;font-family:&quot;:,sans-serif;color:#595959">&nbsp;</span></i><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  <p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
  </td> </tr>
 +
</table>
 +
<p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
<p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 +
<p align="left" style="margin:4.8pt 0cm 6pt"><span style="font-size:11pt;font-family:Calibri, sans-serif">&nbsp;</span><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p>
 
= <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:16.0pt;font-family:&quot;:,sans-serif;color:#1E4E79">Cry4Aa4+Extended2A+Cyt1-pChlamy3</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> =
 
= <p align="left" style="margin:4.8pt 0cm 6pt"><b><span style="font-size:16.0pt;font-family:&quot;:,sans-serif;color:#1E4E79">Cry4Aa4+Extended2A+Cyt1-pChlamy3</span></b><span style="font-size:10pt;font-family:Tahoma, sans-serif"></span></p> =
  

Revision as of 20:55, 19 October 2016

Team FAFU-CHINA

Cry4Aa4+Extended2A+Cyt2-pChlamy3

Gene Name

Cry4Aa4+Extended2A+Cyt2

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in Top10

Gene Length

4461bp

Cloning Strategy

XbaI and PstI

 

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

 

T--FAFU-CHINA--bs13.png

 

T--FAFU-CHINA--bs14.png

 

 

Lane M: DNA Marker

 

Lane 1: Plasmid digested by XbaI and BamHI

 

Lane 2: Plasmid DNA

 

 

 

 

 

 

Cry4Aa4+Extended2A+Cyt1-pChlamy3

Gene Name

Cry4Aa4+Extended2A+Cyt1-pChlamy3

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in JM108

Gene Length

4422bp

Cloning Strategy

XbaI and PstI

 

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

 

T--FAFU-CHINA--bs13.png

T--FAFU-CHINA--bs16.png

 

Lane M: DNA Marker

 

Lane 1: Plasmid digested by BamHI and KpnI

 

Lane 2: Plasmid DNA

 

 

 

Cry10Aa4+Extended2A+Cyt2-pChlamy3

 

Gene Name

Cry10Aa4+Extended2A+Cyt2-pChlamy3

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in DH5α

Gene Length

2946bp

Cloning Strategy

XbaI and PstI

 

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

 

T--FAFU-CHINA--bs13.png

T--FAFU-CHINA--bs18.png

 

Lane M: DNA Marker

 

Lane 1: Plasmid digested by XbaI and PstI

 

Lane 2: Plasmid DNA

 

 

 

 

 

Cry10Aa4+Extended2A+Cyt2-pChlamy3

Gene Name

Cry10Aa4+Extended2A+Cyt2-pChlamy3

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in DH5α

Gene Length

2946bp

Cloning Strategy

XbaI and PstI

 

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

 

T--FAFU-CHINA--bs3.png

 

T--FAFU-CHINA--bs20.png

 

Lane M: DNA Marker

 

Lane 1: Plasmid digested by XbaI and PstI

 

Lane 2: Plasmid DNA

 

 

 

Cry10Aa4+Extended2A+Cyt1-pChlamy3

Gene Name

Cry10Aa4+Extended2A+Cyt1-pChlamy3

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in DH5α

Gene Length

2907bp

Cloning Strategy

XbaI and PstI

 

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

T--FAFU-CHINA--bs3.png

 

T--FAFU-CHINA--bs22.png

 

Lane M: DNA Marker

 

Lane 1: Plasmid digested by XbaI and PstI

 

Lane 2: Plasmid DNA

 

 

 

Cry11Aa4+Extended2A+Cyt2-pChlamy3

Gene Name

Cry11Aa4+Extended2A+Cyt2-pChlamy3

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in Top10

Gene Length

2859bp

Cloning Strategy

XbaI and PstI

 

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

 

T--FAFU-CHINA--bs3.png

T--FAFU-CHINA--bs24.png

 

Lane M: DNA Marker

 

Lane 1: Plasmid digested by XbaI and PstI

 

Lane 2: Plasmid DNA

 

 

Cry11Aa4+Extended2A+Cyt1-pChlamy3

Gene Name

Cry11Aa4+Extended2A+Cyt1-pChlamy3

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in JM108

Gene Length

2820bp

Cloning Strategy

XbaI and PstI

 

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

 

T--FAFU-CHINA--bs3.png

T--FAFU-CHINA--bs26.png

 

Lane M: DNA Marker

 

Lane 1: Plasmid digested by XbaI and PstI

 

Lane 2: Plasmid DNA

 

 

 

 

Cry11Aa4+Extended2A+EGFP-pChlamy3

Gene Name

Cry11Aa4+Extended2A+EGFP-pChlamy3

Cloning Vector

pChlamy_3

Construct Resistant

Ampicillin in JM108

Gene Length

2790bp

Cloning Strategy

XbaI and PstI

 

QC Items

Method

Specifications

Results

Target Sequence

Sequence alignment

Sequencing results are consistent to the confirmed sequence.

Pass

Vector Sequence

Sequence alignment

20bp flanking sequences of the vector are correct.

Pass

Reading Frame

Sequence alignment

Frame is correct and consistent to the client's requirement.

Pass

Fragment Size

Restriction Digests

The size of inserted fragment is right and free of any contaminated bands.

Pass

Quality/Quantity

UV spectrophotometry

Miniprep: OD260/280=1.7~2.0

2µg/tube, 1 tube,Lyophilized

Pass

Type of Strain Stock

Visual inspection

Bacteria stock.

Pass

Appearance

Visual inspection

Clear and free of foreign particles, and the strain is clearly visible.

Pass

 

T--FAFU-CHINA--bs3.png

T--FAFU-CHINA--bs28.png

 

Lane M: DNA Marker

 

Lane 1: Plasmid digested by XbaI and PstI

 

Lane 2: Plasmid DNA