Difference between revisions of "Team:UFAM-UEA Brazil/Judging/MedalRequirements"
| Line 81: | Line 81: | ||
<h3 style="color:#C6A500;margin-left: 2em">2. Improve the function OR characterization of a previously existing BioBrick Part or Device (created by another team, or by your own team in in a previous year of iGEM), and enter this information in the part's page on the Registry. Please see the Registry Contribution help page for help on documenting a contribution to an existing part. This part must NOT come from your team's 2016 range of part numbers.</h3> | <h3 style="color:#C6A500;margin-left: 2em">2. Improve the function OR characterization of a previously existing BioBrick Part or Device (created by another team, or by your own team in in a previous year of iGEM), and enter this information in the part's page on the Registry. Please see the Registry Contribution help page for help on documenting a contribution to an existing part. This part must NOT come from your team's 2016 range of part numbers.</h3> | ||
| − | <p> | + | <p>Bba_K1355004 was one of the best parts (at least for us) from our past project from UFAM_Brazil team from 2014. It has a bi-directional promoter regulated by mercury, and upstream to it there is a merR gene (repressor) and downstream we have transporter genes merT and merP besides the mercury reductase gene (merA). We improved this Device by deleting the bi-directional promoter and added two other ones: a constitutive promoter (from Anderson promoter collection - Bba_J23100) and a new promoter (new from the Registry) the JK26 under the name of . Also we added the merB gene, a lyase that cleave methyl radical from the CH3Hg, turning it to Hg2+ being prompt to be reduced to Hg0 by MerA (which is included in the Device). MerB also gives more activity spectrum to MerA. Take a look on the complete description. </p> |
<br/> | <br/> | ||
Revision as of 00:19, 20 October 2016
JUDGING
Medal Requirements
Click on a medal to jump to its section.
Bronze Requirements
1. Register the team, have a great summer, and plan to have fun at the Giant Jamboree.
Resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta.
2. Meet all deliverables on the Requirements page
Resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta.
3. Create a page on your team wiki with clear attribution of each aspect of your project. This page must clearly attribute work done by the students and distinguish it from work done by others, including host labs, advisors, instructors, sponsors, professional website designers, artists, and commercial services.
Resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta.
4. Document at least one new standard BioBrick Part or Device central to your project and submit this part to the iGEM Registry (submissions must adhere to the iGEM Registry guidelines). You may also document a new application of a BioBrick part from a previous iGEM year, adding that documentation to the part's main page.
Resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta.
Silver Requirements
1. Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected. Document the characterization of this part in the Main Page section of the Registry entry for that Part/Device. This working part must be different from the part you documented in Bronze medal criterion #4. Submit this part to the iGEM Parts Registry.
Resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta.
2. Convince the judges you have helped any registered iGEM team from a high-school, different track, another university, or institution in a significant way by, for example, mentoring a new team, characterizing a part, debugging a construct, modeling/simulating their system or helping validate a software/hardware solution to a synthetic biology problem.
Resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta.
3. iGEM projects involve important questions beyond the bench, for example relating to (but not limited to) ethics, sustainability, social justice, safety, security, and intellectual property rights. We refer to these activities as Human Practices in iGEM. Demonstrate how your team has identified, investigated and addressed one or more of these issues in the context of your project. (See the Human Practices Hub for more information.)
Resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta.
Gold Requirements
1. Expand on your silver medal activity by demonstrating how you have integrated the investigated issues into the design and/or execution of your project.
Resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta resposta.
2. Improve the function OR characterization of a previously existing BioBrick Part or Device (created by another team, or by your own team in in a previous year of iGEM), and enter this information in the part's page on the Registry. Please see the Registry Contribution help page for help on documenting a contribution to an existing part. This part must NOT come from your team's 2016 range of part numbers.
Bba_K1355004 was one of the best parts (at least for us) from our past project from UFAM_Brazil team from 2014. It has a bi-directional promoter regulated by mercury, and upstream to it there is a merR gene (repressor) and downstream we have transporter genes merT and merP besides the mercury reductase gene (merA). We improved this Device by deleting the bi-directional promoter and added two other ones: a constitutive promoter (from Anderson promoter collection - Bba_J23100) and a new promoter (new from the Registry) the JK26 under the name of . Also we added the merB gene, a lyase that cleave methyl radical from the CH3Hg, turning it to Hg2+ being prompt to be reduced to Hg0 by MerA (which is included in the Device). MerB also gives more activity spectrum to MerA. Take a look on the complete description.
3. Demonstrate a functional proof of concept of your project. Your proof of concept must consist of a BioBrick device; a single BioBrick part cannot constitute a proof of concept. (Remember, biological materials may not be taken outside the lab.)
The mercury bioremediation in laboratory conditions using Petri dishes, Erlenmeyer and shakers was a heck of accomplishment. However, taking this to fermentation level in a real bioreactor was the master piece! Even more when this physical device (the bioreactor) was made in the DIY (Do it Yourself) style, using an old autoclave and buying pieces in a mechanical shop. Off course we had help of experts, and learned a lot form them and in the process. You can check our bioreactor at .
4. Show your project working under real-world conditions. To achieve this criterion, you should demonstrate your whole system, or a functional proof of concept working under simulated conditions in the lab (Remember, biological materials may not be taken outside the lab.)
In our attempt to save Amazon’s ecosystem through synthetic biology, we developed genetic circuits in bacteria, enabling them to detect and bioremediate mercury contamination. Our work has tackled this challenge building a set of mechanisms where the first was 1) a library of promoters regulated by mercury; 2)the construction of a novel phytochelatin expressed at the membrane level in E. coli; 3) a brand-new bacteria to remediate contamination; 4) sequence a wild bacteria resistant to mercury; and for final 5)we built the first bioreactor to treat mercury-contaminated waste in the competition. Now the best news! We had a great accomplishment! We have got close to 100% (97%) of mercury degradation in 18 hours of bacterial growth. In the Bioreactor we had 50% Hg degradation in the first 18 hours.
