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− | + | <h2 class="carousel-title bounceInDown animated slow">TU Delft 2016</h2> | |
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+ | <h3 class="col-title">Our Team</h3> | ||
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− | + | <p style="font-family: Arial Black font-weight: 900">We use DNA from sponges to create a little glass-like layer around our cells.</p> | |
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− | + | <h3 class="reasons-title"><a href="https://2016.igem.org/Team:TU_Delft/Project#description">BIOLASERS</a></h3> | |
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+ | <p> <strong>Imaging cells </strong>is essential for understanding life at the smallest scale and fighting cellular diseases like cancer. Imaging often relies on <strong>fluorescence</strong>, but fluorescent proteins have some drawbacks, such as their wide spectrum and low intensity.</p> | ||
+ | <p> Our <strong>biolasers</strong> will provide an accurate, safe and biological way to improve this.</p> | ||
+ | <p> Fluorescence is the ability of a molecule to take up the energy of a photon and release it again, which makes the molecule light up.<strong> Lasing</strong> works with the same principle as fluorescence, but now the light source is put between <strong>mirrors</strong>. The photons keep <strong>‘bouncing’</strong>, increasing the energy of the system. When the light gets a certain power, the photons can escape in the form of a laser beam.</p> | ||
+ | <p>A biolaser is achieved by trapping fluorescent proteins inside a <strong>reflective agent</strong>. We have chosen two reflective agents: bioglass (polysilicate) and bioplastic (PHB). By covering a cell with <strong>polysilicate</strong>, the photons can resonate inside the cell, making a whole-cell laser. The polysilicate is synthesized by an enzyme called <strong>silicatein</strong>, which is expressed on the cell wall by fusion to membrane proteins. By filling a cell with <strong>PHB</strong>, which forms intracellular granules, the photons can resonate inside a part of the cell, making an intracellular laser. The PHB is synthesized after expressing the pha-operon. By fusing the GFP to the PHB synthase, the GFP is relocated into the <strong>PHB granules</strong>.</p> | ||
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+ | <img src="https://static.igem.org/mediawiki/2016/7/71/TU_Delft_frontlens.png" alt="lenses"> | ||
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+ | <p><strong>Microlenses</strong> are an emerging field in technology and have a ton of applications, including high-tech cameras, chips, solar panels and research & imaging techniques. However, they are expensive, hard to fabricate and the production uses heavy chemicals and high temperatures, so it is bad for the <strong>environment</strong>. </p> | ||
+ | <p>Our biological microlens will be cheap, easy to make and environmentally friendly.</p> | ||
+ | <p>When we cover a cell with <strong>polysilicate</strong>, using the enzyme <strong>silicatein</strong>, we are able to make a biological microlens. By overexpressing either the transcriptional regulator bolA or the cell division inhibitor sulA we can play with cell <strong>morphology</strong> and investigate <strong>optical properties</strong>. These enlarged cells can also be used in the lasing experiments. The single cell will be able to diffract light as a <strong>single microlens</strong>. When we make a grid of lenses, a <strong>microlens array</strong>, we can use the lens for a coating for solar panels, thin lightweight cameras with high resolution or 3D screens.</p> | ||
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Revision as of 16:01, 2 August 2016
![](https://static.igem.org/mediawiki/2016/3/38/T--TU_Delft--DidYouKnow.png)
We use DNA from sponges to create a little glass-like layer around our cells.