Difference between revisions of "Team:Manchester"
| Line 171: | Line 171: | ||
padding:0; | padding:0; | ||
} | } | ||
| + | |||
| + | p{ | ||
| + | font-size: 1em; | ||
| + | } | ||
| + | } | ||
| + | |||
| + | body{ | ||
| + | width:100%; | ||
| + | margin:auto; | ||
| + | } | ||
.boxx{ | .boxx{ | ||
| Line 178: | Line 188: | ||
</style> | </style> | ||
| − | |||
<title>IGEM Manchester Team 2016</title> | <title>IGEM Manchester Team 2016</title> | ||
<meta charset="utf-8"> | <meta charset="utf-8"> | ||
<meta name="viewport" content="width=device-width, initial-scale=1"> | <meta name="viewport" content="width=device-width, initial-scale=1"> | ||
</head> | </head> | ||
| − | |||
<body> | <body> | ||
| Line 199: | Line 207: | ||
<div class="boxx"> | <div class="boxx"> | ||
<!--intro text--> | <!--intro text--> | ||
| − | + | <div class="column full_size" > | |
<div id="cntdwn"></div> | <div id="cntdwn"></div> | ||
| Line 206: | Line 214: | ||
<br /> | <br /> | ||
<p >Welcome to the University of Manchester’s iGEM 2016 Wiki page! We are a team of 11 undergraduate students all studying at the The University of Manchester and Manchester Metropolitan University based in the Manchester Institute of Biotechnology under the supervision of Professor Rainer Breitling and Professor Eriko Takano.</p> | <p >Welcome to the University of Manchester’s iGEM 2016 Wiki page! We are a team of 11 undergraduate students all studying at the The University of Manchester and Manchester Metropolitan University based in the Manchester Institute of Biotechnology under the supervision of Professor Rainer Breitling and Professor Eriko Takano.</p> | ||
| + | <p> | ||
<br /><br /><br /> | <br /><br /><br /> | ||
<center> | <center> | ||
| Line 215: | Line 224: | ||
<h4>Science Behind AlcoPatch</h4> | <h4>Science Behind AlcoPatch</h4> | ||
| − | <p>Controlling alcohol consumption can be difficult, especially amongst students in the UK. An alcohol patch that generates a visible colour change according to blood alcohol concentration could be used to monitor the level of intoxication in a person. Having an indicator to show how intoxicated a person is could help reduce undesired consequences of being too drunk </p> | + | <p >Controlling alcohol consumption can be difficult, especially amongst students in the UK. An alcohol patch that generates a visible colour change according to blood alcohol concentration could be used to monitor the level of intoxication in a person. Having an indicator to show how intoxicated a person is could help reduce undesired consequences of being too drunk </p> |
<h4>Our Project Plan </h4> | <h4>Our Project Plan </h4> | ||
| − | <p>It is known that the ethanol concentration in sweat is linearly related to the blood alcohol concentration. Based on this, we aim to develop an ethanol biosensor by using synthetic biology with two separate methods to measure intoxication levels. The first mechanism involves the usage of alcohol oxidase (AOX) in a cell-free system to oxidise ethanol to acetaldehyde that produces hydrogen peroxide <i>H<sub>2</sub>O<sub>2</sub></i> as a by-product. <i>H<sub>2</sub>O<sub>2</sub></i> is used as an oxidising agent by horseradish peroxidase (HRP) to convert ABTS to produce luminescence. The second mechanism involves activation of ethanol sensitive alcR in engineered Escherichia Coli (E.Coli). The activated transcription factor then activates the promoter alcA leading to the expression of chromoprotein. | + | <p style="font-size:1em">It is known that the ethanol concentration in sweat is linearly related to the blood alcohol concentration. Based on this, we aim to develop an ethanol biosensor by using synthetic biology with two separate methods to measure intoxication levels. The first mechanism involves the usage of alcohol oxidase (AOX) in a cell-free system to oxidise ethanol to acetaldehyde that produces hydrogen peroxide <i>H<sub>2</sub>O<sub>2</sub></i> as a by-product. <i>H<sub>2</sub>O<sub>2</sub></i> is used as an oxidising agent by horseradish peroxidase (HRP) to convert ABTS to produce luminescence. The second mechanism involves activation of ethanol sensitive alcR in engineered Escherichia Coli (E.Coli). The activated transcription factor then activates the promoter alcA leading to the expression of chromoprotein. |
</p> | </p> | ||
| + | <br /> | ||
| + | <!-- | ||
| + | <div class="row"> | ||
| + | <div class="alert"> | ||
| + | <h3> Support our project by donating through <a href="http://experiment.com/projects/alcopatch-a-new-way-of-detecting-alcohol-intoxication" target="blank"> Experiment.com</a> by 26 August 2016! </h3> | ||
<br /> | <br /> | ||
| + | <h3>This will help us tremendously and would enable us to present our project in Boston on the <a style="color:green" href="https://2016.igem.org/Giant_Jamboree" target="blank">iGEM Giant Jamboree</a> : where one of the largest synthetic biology competition <a style="color:green" href="https://2016.igem.org/Main_Page" target="blank">(iGEM)</a> will take place!</h3> | ||
| + | |||
| + | <br /> | ||
| + | |||
| + | <h3>We, the <b style="purple">Manchester iGEM team 2016</b> appreciate so much for your kind support!</h3> | ||
| + | |||
| + | </div> | ||
| + | <div class="alert1"> | ||
| + | <a href="https://experiment.com/projects/alcopatch-a-new-way-of-detecting-alcohol-intoxication" target="blank"> <img src="https://static.igem.org/mediawiki/2016/0/0f/T--Manchester--Group_Photos.png"></img></a> | ||
| + | </div> | ||
| + | </div> | ||
| + | --> | ||
<div class="social"> | <div class="social"> | ||
| Line 229: | Line 255: | ||
<p> | <p> | ||
Follow our activity on: | Follow our activity on: | ||
| − | |||
<br /> | <br /> | ||
<br /> | <br /> | ||
| Line 239: | Line 264: | ||
<br /><br /><br /><br /><br /><br /><br /><br /> | <br /><br /><br /><br /><br /><br /><br /><br /> | ||
</div> | </div> | ||
| − | |||
</div> | </div> | ||
| + | |||
| + | </div> | ||
<!--change display to half column--> | <!--change display to half column--> | ||
| Line 316: | Line 342: | ||
UPLOAD FILES | UPLOAD FILES | ||
</div> | </div> | ||
| − | |||
| + | </div> | ||
| + | --> | ||
Revision as of 16:20, 2 September 2016
Welcome to iGEM 2016!
Welcome to the University of Manchester’s iGEM 2016 Wiki page! We are a team of 11 undergraduate students all studying at the The University of Manchester and Manchester Metropolitan University based in the Manchester Institute of Biotechnology under the supervision of Professor Rainer Breitling and Professor Eriko Takano.
Project Description
Science Behind AlcoPatch
Controlling alcohol consumption can be difficult, especially amongst students in the UK. An alcohol patch that generates a visible colour change according to blood alcohol concentration could be used to monitor the level of intoxication in a person. Having an indicator to show how intoxicated a person is could help reduce undesired consequences of being too drunk
Our Project Plan
It is known that the ethanol concentration in sweat is linearly related to the blood alcohol concentration. Based on this, we aim to develop an ethanol biosensor by using synthetic biology with two separate methods to measure intoxication levels. The first mechanism involves the usage of alcohol oxidase (AOX) in a cell-free system to oxidise ethanol to acetaldehyde that produces hydrogen peroxide H2O2 as a by-product. H2O2 is used as an oxidising agent by horseradish peroxidase (HRP) to convert ABTS to produce luminescence. The second mechanism involves activation of ethanol sensitive alcR in engineered Escherichia Coli (E.Coli). The activated transcription factor then activates the promoter alcA leading to the expression of chromoprotein.
