Difference between revisions of "Team:Manchester/Description"

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   <p style="font-size: 20px;text-align:left">The <i>alc</i> gene expression system is one of the most reliable chemically inducible gene switches for use in plants and fungus.    This system relies on the ability of AlcR, a transcription factor, to bind to its target <i>alcA</i> promoter (alcA<sup>P</sup>). Based on this, we have engineered  <i>Escherichia coli</i> K-12 derivative DH5α and BL21 to induce expression of chromoproteins when AlcR binds to alcA<sup>P</sup> in the presence of ethanol <sup>[1]</sup>.  
 
   <p style="font-size: 20px;text-align:left">The <i>alc</i> gene expression system is one of the most reliable chemically inducible gene switches for use in plants and fungus.    This system relies on the ability of AlcR, a transcription factor, to bind to its target <i>alcA</i> promoter (alcA<sup>P</sup>). Based on this, we have engineered  <i>Escherichia coli</i> K-12 derivative DH5α and BL21 to induce expression of chromoproteins when AlcR binds to alcA<sup>P</sup> in the presence of ethanol <sup>[1]</sup>.  
 
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   <div class="directlink"><h1><a href="https://2016.igem.org/Team:Manchester/Description/mechanism2">Click here for more info</a></h1></div>
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Revision as of 16:57, 28 September 2016

Manchester iGEM 2016

Project Overview




Mechanism 1

Cell Free Mechanism


Mechanism 2 overview diagram









Mechanism 2

Inducible Gene Switch


Mechanism 2 overview diagram


The alc gene expression system is one of the most reliable chemically inducible gene switches for use in plants and fungus. This system relies on the ability of AlcR, a transcription factor, to bind to its target alcA promoter (alcAP). Based on this, we have engineered Escherichia coli K-12 derivative DH5α and BL21 to induce expression of chromoproteins when AlcR binds to alcAP in the presence of ethanol [1].

Click here for more info

Reference

  • Panozzo, C., Capuano, V., Fillinger, S. and Felenbok, B. (1997) ‘The zinc binuclear cluster Activator AlcR is able to bind to single sites but requires multiple repeated sites for synergistic activation of the alcA gene in Aspergillus nidulans’, Journal of Biological Chemistry, 272(36), pp. 22859–22865.

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