Difference between revisions of "Team:Manchester/Parts"

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<td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K2092002 " target="_blank">http://parts.igem.org/Part:BBa_K2092002 </a></td>
 
<td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K2092002 " target="_blank">http://parts.igem.org/Part:BBa_K2092002 </a></td>
 
<td style="border:1px black solid;">PalcA</td>
 
<td style="border:1px black solid;">PalcA</td>
<td style="border:1px black solid;">This part contains the ethanol/alcR regulated  alcA promoter, the native variant of alcA promoter from Aspergillus nidulans which tightly regulates gene expression. It is an improved BioBrick from 2011 iGEM DTU-Denmark <a href="http://parts.igem.org/Part:BBa_K678001" target="_blank">BBa_K678001</a> </td>
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<td style="border:1px black solid;">This part contains the ethanol/alcR regulated  alcA promoter, the native variant of alcA promoter from Aspergillus nidulans which tightly regulates gene expression. It is an improved BioBrick from 2011 iGEM DTU-Denmark <a href="http://parts.igem.org/Part:BBa_K678001" target="_blank"> (BBa_K678001)</a> </td>
 
<td style="border:1px black solid;">Basic</td>
 
<td style="border:1px black solid;">Basic</td>
 
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<td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K2092003" target="_blank">http://parts.igem.org/Part:BBa_K2092003 </a></td>
 
<td style="border:1px black solid;"><a href="http://parts.igem.org/Part:BBa_K2092003" target="_blank">http://parts.igem.org/Part:BBa_K2092003 </a></td>
 
<td style="border:1px black solid;">PalcA(var)</td>
 
<td style="border:1px black solid;">PalcA(var)</td>
<td style="border:1px black solid;">This part contains the ethanol/alcR regulated alcA promoter, an engineered variant of the alcA promoter from Aspergillus nidulans which tightly regulates gene expression.  The lack of one binding site on this promoter is suggested to have 70% decrease in promoter strength in comparison to the native <a href="http://parts.igem.org/Part:BBa_K2092003" target="_blank">BBa_K2092002</a>.</td>
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<td style="border:1px black solid;">This part contains the ethanol/alcR regulated alcA promoter, an engineered variant of the alcA promoter from Aspergillus nidulans which tightly regulates gene expression.  The lack of one binding site on this promoter is suggested to have 70% decrease in promoter strength in comparison to the native PalcA<a href="http://parts.igem.org/Part:BBa_K2092003" target="_blank"> (BBa_K2092002)</a>.</td>
 
<td style="border:1px black solid;">Basic</td>
 
<td style="border:1px black solid;">Basic</td>
 
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</tr>

Revision as of 23:04, 9 October 2016

Manchester iGEM 2016

Parts

BioBrick Link Part Name Description Part Type
BBa_K2092000 http://parts.igem.org/Part:BBa_K2092000 AOx without RBS This part encodes for the AOx1 protein from Pichia Pastoris. This enzyme oxidises primary alcohols to produce aldehydes and hydrogen peroxide. It is designed with an NDeI restriction site between the prefix and the start of the AOx gene. Basic
BBa_K2092001 http://parts.igem.org/Part:BBa_K2092001 alcR without RBS/scar This part encodes for AlcR protein, an ethanol-activated transcriptional factor. It binds to alcA promoter and aldA promoter to induce expression of proteins in the presence of ethanol or threonine. Basic
BBa_K2092002 http://parts.igem.org/Part:BBa_K2092002 PalcA This part contains the ethanol/alcR regulated alcA promoter, the native variant of alcA promoter from Aspergillus nidulans which tightly regulates gene expression. It is an improved BioBrick from 2011 iGEM DTU-Denmark (BBa_K678001) Basic
BBa_K2092003 http://parts.igem.org/Part:BBa_K2092003 PalcA(var) This part contains the ethanol/alcR regulated alcA promoter, an engineered variant of the alcA promoter from Aspergillus nidulans which tightly regulates gene expression. The lack of one binding site on this promoter is suggested to have 70% decrease in promoter strength in comparison to the native PalcA (BBa_K2092002). Basic
BBa_K2092004 http://parts.igem.org/Part:BBa_K2092004 alcR (incl. RBS) This part is a twin of BBa_K2092001 with an additional RBS ( BBa_J34801) upstream of the start codon. This part encodes for AlcR protein, an ethanol-activated transcriptional factor from Aspergillus nidulans. It binds to alcA (BBa_K2092002) and aldA promoters to induce expression of proteins in the presence of ethanol or threonine. Composite
BBa_K2092006 http://parts.igem.org/Part:BBa_K2092006 CP3+RBS+amilCP This part consists of a constitutive promoter (BBa_J23110), RBS (BBa_B0034) and blue chromoprotein amilCP (BBa_K592009). Suitable to be used as a reporter. Composite
BBa_K2092008 http://parts.igem.org/Part:BBa_K2092008 CP3+RBS+alcR This part consists of a constitutive promoter (BBa_J23110), RBS (BBa_B0034) and blue chromoprotein amilCP (BBa_K592009). Suitable to be used to measure the promoter strengths of alcA/aldA. Composite
BBa_K2092009 http://parts.igem.org/Part:BBa_K2092009 PalcA var+RBS+amilCP This part consists of PalcA var (BBa_K2092003) tagged with blue chromoprotein (BBa_K592009), which makes it capable to produce blue colour upon the binding of its ethanol-activated transcription factor AlcR. Composite