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Revision as of 04:37, 12 October 2016
tetp Module
The tetp module consists of a Tet repressible promoter upstream of the CDSs of phlf and lacI, which are then followed by the reporter gene, mtagbfp. When the ptet module is active, the two repressor proteins, PhlF and LacI will be expressed along with the reporter gene, mTagBFP. This results in the repression of the two other modules in our system, pphlf and plac.
Promoter, Repressor and Inducer Specification
- Promoter name: tetp (Tet repressible promoter)
- Length: 54 base pairs
- Strength with:
- Weak RBS (BBa_B0032): 5.6 - 517.5 (+17) RPU
- Medium RBS (BBa_B0030): 25.5 - 2323.1 (+259.5) RPU
- Strong RBS (BBa_B0034): 39.6 - 4041.5 (+247.8) RPU
In gram negative bacteria, TetR represses tetp which regulates tetracycline resistance by driving the expression of TetR and TetA, the latter of which codes for a tetracycline-magnesium complex that pumps tetracycline out of the cell.
- Repressor name: TetR (Tet repressor)
- Part Length: 685 base pairs
- Structure: Homodimer of alpha-helices
- Binding affinity: To tetp, very light, 0-1 µl/ml has been shown to cause a 5 order of magnitude change in fluorescent protein production
- Inducer name: ATc (Anhydrotetracycline)
- Binding Affinity: (to TetR) Ka ~ 109 M-1
- Effective range: 250 - 25000 ng/ml