Difference between revisions of "Team:Northwestern/06 20"

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               <li>Diluted 5 tubes of cells up to 5mL in culture tubes at 10:30am</li>
 
               <li>Diluted 5 tubes of cells up to 5mL in culture tubes at 10:30am</li>
 
               <li>Removed at 2:20 at OD<sub>600</sub>=0.37</li>
 
               <li>Removed at 2:20 at OD<sub>600</sub>=0.37</li>
−
               <li>Resuspended plasmid DNA in 100mL of dH2O, vortexed, spun down</li>
+
               <li>Resuspended practice plasmid DNA in 100mL of dH2O, vortexed, spun down</li>
 
               <li>Transformed cells with 5&#956;L of resuspended DNA</li>
 
               <li>Transformed cells with 5&#956;L of resuspended DNA</li>
 
             </ul>
 
             </ul>

Revision as of 05:28, 12 October 2016

Notebook

Monday, June 20th

Agenda:

  • Make TSS Buffer
  • Make Competent Cells
  • Transform parts out of the iGEM registry

Tasks:

Jordan, Paul, & Shu

  • Made TSS
    • 5g of PEG 8000
    • 2.5mL of DMSO
    • 1.5mL of 1M MgCl
  • Filtered LB
    • Used 10mL syringe filter to sterilize
    • Stored on ice
  • Made QuickCompetent Cells
    • Diluted 5 tubes of cells up to 5mL in culture tubes at 10:30am
    • Removed at 2:20 at OD600=0.37
    • Resuspended practice plasmid DNA in 100mL of dH2O, vortexed, spun down
    • Transformed cells with 5μL of resuspended DNA
  • Followed iGEM transformation procedures
    • Incubated for 1hr 45min