Difference between revisions of "Team:Manchester/Notebook"

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   <button class="hideall">Hide All Recipes</button>
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   <button class="hideall">Hide All</button>
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   <button class="expandall">Expand All Recipes</button>
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   <button class="expandall">Expand All</button>
  
  
 
<!------------------------------------------------------Week 1----------------------------------------------------->
 
<!------------------------------------------------------Week 1----------------------------------------------------->
  
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<button type="button" class="week1button">Week 1 (4<sup>th</sup> to 8<sup>th</sup> of July)</button>
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<button type="button" class="week1button">Week 1 (29<sup>th</sup> June to 1<sup>st</sup> of July)</button>
  
  
 
   <div class="week1">
 
   <div class="week1">
 
     <div class="geneswitch">
 
     <div class="geneswitch">
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     <h1 class="subnote">Inducible Gene Switch</h1>
 
     <h1 class="subnote">Inducible Gene Switch</h1>
 +
 +
      <ul class="list">
 +
        <li>Re-suspended DNA (constitutive promoters) from iGEM Distribution kit and transformed them into DH5α strain. </li>
 +
        <li>Growth was found on the negative control plates so re-transformed DNA</li>
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        <li>Inoculated of transformed cells. </li>
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      </ul>     
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    </div>
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  </div>
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<!------------------------------------------------------Week 2----------------------------------------------------->
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<button type="button" class="week2button">Week 2 (4<sup>th</sup> to 8<sup>th</sup> of July)</button>
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    <div class="pilot">
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    <h1 class="subnote">Pilot Experiment</h1>
  
 
       <ul class="list">
 
       <ul class="list">
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     </div>
 
     </div>
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    <div class="geneswitch">
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    <h1 class="subnote">Inducible Gene Switch</h1>
 +
 +
      <ul class="list">
 +
        <li>Performed miniprep for overnight cultures</li>
 +
        <li>Prepared fresh batch of DH5α chemical competent cells</li>
 +
        <li>Restriction enzyme digest of constitutive promoters and control(pUC19) for validation with EcoRI and PstI</li>
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      </ul>
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    </div>
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   </div>
 
   </div>
  
  
  
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<!------------------------------------------------------Week 3----------------------------------------------------->
  
  
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<button type="button" class="week3button">Weel 3 (11<sup>th</sup> to 15<sup>th</sup> of July</button>
  
  
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  <div class="week2">
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    <div class="pilot">
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    <h1 class="subnote">Pilot Experiment</h1>
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      <ul class="list">
 +
        <li>Still awaiting the arrival of the BMG spectrophotometer </li>
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      </ul>
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    </div>
 +
 +
 +
    <div class="geneswitch">
 +
 +
    <h1 class="subnote">Inducible Gene Switch</h1>
 +
 +
      <ul class="list">
 +
        <li>Prepared DH5α chemical competent cells</li>
 +
        <li>Prepared Chloramphenicol antibiotic stock</li>
 +
        <li>Restreaked alcA 1 (BBa_K678001) and spispink(BBa_K1033923 pink chromoprotein)</li>
 +
      </ul>
 +
 
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    </div>
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  </div>
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<button type="button" class="week2button"></button>
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  <div class="week2">
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    <div class="pilot">
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    <h1 class="subnote">Pilot Experiment</h1>
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      <ul class="list">
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        <li></li>
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      </ul>
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    </div>
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 +
 +
    <div class="geneswitch">
 +
 +
    <h1 class="subnote">Inducible Gene Switch</h1>
 +
 +
      <ul class="list">
 +
        <li></li>
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        <li></li>
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        <li></li>
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      </ul>
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    </div>
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  </div>
  
  

Revision as of 20:48, 13 October 2016

Manchester iGEM 2016

Notebook

Inducible Gene Switch

  • Re-suspended DNA (constitutive promoters) from iGEM Distribution kit and transformed them into DH5α strain.
  • Growth was found on the negative control plates so re-transformed DNA
  • Inoculated of transformed cells.

Pilot Experiment

  • Made stocks of our reagents Glucose Stock for 0.005 g/mL, Glucose Oxidase Stock for 0.0025 g/mL, HRP Stock for 250 μL/mL, ABTS for 0.0025 g/mL

Inducible Gene Switch

  • Performed miniprep for overnight cultures
  • Prepared fresh batch of DH5α chemical competent cells
  • Restriction enzyme digest of constitutive promoters and control(pUC19) for validation with EcoRI and PstI

Pilot Experiment

  • Still awaiting the arrival of the BMG spectrophotometer

Inducible Gene Switch

  • Prepared DH5α chemical competent cells
  • Prepared Chloramphenicol antibiotic stock
  • Restreaked alcA 1 (BBa_K678001) and spispink(BBa_K1033923 pink chromoprotein)

Pilot Experiment

Inducible Gene Switch

Sponsors

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