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Revision as of 21:38, 14 October 2016

</div> </div> Modeling

Parts Collection
BioBrick Devices Submitted to the Registry
Furthering collaboration and standardization of genetic parts

sMMO (BBa_K2032000)

Contains the subunits necessary to assembly the soluble methane monooxygenase (sMMO) enzyme complex. Contains MMO subunits B, C, D, X, Y, and Z, each preceded by Anderson rbs B0032, and all under lactose of IPTG inducable control. The sMMO complex oxidizes methane into methanol

Fructose Pathway (BBa_K2032001)

Contains genes MDH, HPS, and PHI. Methanol Dehydrogenase II converts methanol into formate. HPS and PHI turn it into metabolites that feed into the glycolysis pathway.

Formate Pathway (BBa_K2032002)

Contains stop codons after parts FDH and FALDH! Converts methanol into carbon dioxide, generating energy in the form of NADPH in the process.

Codon optimized MDH2 with Lac/pL promoter (BBa_K2032003)

Codon optimized MDH2 with lac + pL promoter

GroESL (BBa_K2032004)

Chaperone complex

GroESL Composite (BBa_K2032005)

Contains promoter, rbs, and terminator along with GroESL coding region

Fructose with RFP (BBa_K2032006)

Fructose construct with RFP reporter

Formate with RFP (BBa_K2032007)

Formate construct with RFP reporter

sMMO and GFP (BBa_K2032008)

sMMO and GFP for coculture test with fructose/formate and RFP