Difference between revisions of "Team:Northwestern/09 28"

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     <h1>Wednesday, September 28<sup>th</sup></h3>
 
     <h1>Wednesday, September 28<sup>th</sup></h3>
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     <h2>Agenda:</h2>
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     <h2>Tasks:</h2>
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        <p>Jordan</p>
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          <li>Colony PCR all pET28a previously picked colonies</li>
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          <li>Transform SS-Cas9 in tet plasmid into JC8031 </li>
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        <p>Sam</p>
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          <li>Autoclave 5 beveled Erlenmeyers LB for 25 mL cultures, also all of the tips</li>
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          <li>Rerun the PCR from yesterday that Paul did (SS-cas9 with res digest overhangs)</li>
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          <li>Make extra plates of the TorA and YcdO colonies 1 and 2 </li>
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Revision as of 05:02, 16 October 2016

Notebook

Wednesday, September 28th

Tasks:

Jordan

  • Colony PCR all pET28a previously picked colonies
  • Transform SS-Cas9 in tet plasmid into JC8031

Sam

  • Autoclave 5 beveled Erlenmeyers LB for 25 mL cultures, also all of the tips
  • Rerun the PCR from yesterday that Paul did (SS-cas9 with res digest overhangs)
  • Make extra plates of the TorA and YcdO colonies 1 and 2