Difference between revisions of "Team:OUC-China/Protocol"

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<li><a href="https://2016.igem.org/Team:OUC-China">Home</a></li>
 
<li><a href="https://2016.igem.org/Team:OUC-China">Home</a></li>
<li><a href="https://2016.igem.org/Team:OUC-China/Project">Project</a></li>
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<li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Project<span class="caret"></span></a>
<li><a href="https://2016.igem.org/Team:OUC-China/Model">Model</a></li>
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<ul class="dropdown-menu">
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<li><a ref="https://2016.igem.org/Team:OUC-China/Project">Introduction</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Design">Design</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Proof">Proof of concept </a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Results">Results</a></li>
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<li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Parts<span class="caret"></span></a>
 
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<li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Human Practice<span class="caret"></span></a>
<li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Notebook<span class="caret"></span></a>
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<li><a href="https://2016.igem.org/Team:OUC-China/Notebook">Notebook</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Human_Practices">Overview</a></li>
                                                                <li class="active"><a href="#">Protocol</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Communicating_&_improving">Communicating & improving</a></li>
<li><a href="https://2016.igem.org/Team:OUC-China/Safety">Safety</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Investigating_&_promoting">Investigating & promoting</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Potential_application">Potential application</a></li>
 
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<li><a href="https://2016.igem.org/Team:OUC-China/Human_Practices">Human Practices</a></li>
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<li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Team<span class="caret"></span></a>
<li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Others<span class="caret"></span></a>
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<ul class="dropdown-menu">
 
<ul class="dropdown-menu">
<li><a href="https://2016.igem.org/Team:OUC-China/Attributions">Attributions</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Team">Team</a></li>
 
<li><a href="https://2016.igem.org/Team:OUC-China/Collaborations">Collaborations</a></li>
 
<li><a href="https://2016.igem.org/Team:OUC-China/Collaborations">Collaborations</a></li>
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<li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Modeling<span class="caret"></span></a>
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<li><a href="https://2016.igem.org/Team:OUC-China/Model">Model</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Software">Software</a></li>
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</li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Attributions">Attributions</a></li>
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<li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Safety<span class="caret"></span></a>
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<ul class="dropdown-menu">
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<li><a href="https://2016.igem.org/Team:OUC-China/Notebook">Notebook</a></li>
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<li class="active"><a href="https://2016.igem.org/Team:OUC-China/Protocol">Protocol</a></li>
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<li><a href="https://2016.igem.org/Team:OUC-China/Safety">Safety</a></li>
 
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                 <div class="img_banner"><img src="https://static.igem.org/mediawiki/2016/f/fe/T--OUC-China--protocol-banner.jpg" class="img-responsive" alt="protocol-banner"></div>
 
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<h3>1. Elementary Operation</h3>
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<h3>1. Elementary Operation</h3>
<p>
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<p>
1) <a href="https://static.igem.org/mediawiki/2016/1/15/T--OUC-China--Luria-Bertani_medium.pdf">Luria-Bertani liquid and Luria-Bertani solid medium</a><br>
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1) <a href="https://static.igem.org/mediawiki/2016/1/15/T--OUC-China--Luria-Bertani_medium.pdf">Luria-Bertani liquid and Luria-Bertani solid medium</a><br>
2) <a href="https://static.igem.org/mediawiki/2016/0/0c/T--OUC-China--Inoculation.pdf">Inoculation</a><br>
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2) <a href="https://static.igem.org/mediawiki/2016/0/0c/T--OUC-China--Inoculation.pdf">Inoculation</a><br>
3) <a href="https://static.igem.org/mediawiki/2016/d/dc/T--OUC-China--Polymerase_Chain_Reaction.pdf">Polymerase Chain Reaction</a><br>
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3) <a href="https://static.igem.org/mediawiki/2016/d/dc/T--OUC-China--Polymerase_Chain_Reaction.pdf">Polymerase Chain Reaction</a><br>
4) <a href="https://static.igem.org/mediawiki/2016/c/cb/T--OUC-China--Gel_Electrophoresis.pdf">Gel Electrophoresis</a><br>
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4) <a href="https://static.igem.org/mediawiki/2016/c/cb/T--OUC-China--Gel_Electrophoresis.pdf">Gel Electrophoresis</a><br>
5) <a href="https://static.igem.org/mediawiki/2016/0/00/T--OUC-China--Restriction_Enzyme_Digestion.pdf">Restriction Enzyme Digestion</a><br>
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5) <a href="https://static.igem.org/mediawiki/2016/0/00/T--OUC-China--Restriction_Enzyme_Digestion.pdf">Restriction Enzyme Digestion</a><br>
6) <a href="https://static.igem.org/mediawiki/2016/d/df/T--OUC-China--Gel_Extraction.pdf">Gel Extraction</a><br>
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6) <a href="https://static.igem.org/mediawiki/2016/d/df/T--OUC-China--Gel_Extraction.pdf">Gel Extraction</a><br>
7) <a href="https://static.igem.org/mediawiki/2016/1/14/T--OUC-China--Ligation.pdf">Ligation system</a><br>
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7) <a href="https://static.igem.org/mediawiki/2016/1/14/T--OUC-China--Ligation.pdf">Ligation system</a><br>
8) <a href="https://static.igem.org/mediawiki/2016/f/fb/T--OUC-China--Transformation_of_Escherichia_coli.pdf">Transformation of <i>E.coli</i></a><br>
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8) <a href="https://static.igem.org/mediawiki/2016/f/fb/T--OUC-China--Transformation_of_Escherichia_coli.pdf">Transformation of <i>E.coli</i></a><br>
9) <a href="https://static.igem.org/mediawiki/2016/8/84/T--OUC-China--Conservation.pdf">Conservation</a><br>
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9) <a href="https://static.igem.org/mediawiki/2016/8/84/T--OUC-China--Conservation.pdf">Conservation</a><br>
10) <a href="https://static.igem.org/mediawiki/2016/c/c8/T--OUC-China--Sterilization.pdf">Sterilization</a><br>
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10) <a href="https://static.igem.org/mediawiki/2016/c/c8/T--OUC-China--Sterilization.pdf">Sterilization</a><br>
11) <a href="https://static.igem.org/mediawiki/2016/1/1f/T--OUC-China--Plasmid_extraction.pdf">Plasmid extraction</a>
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11) <a href="https://static.igem.org/mediawiki/2016/1/1f/T--OUC-China--Plasmid_extraction.pdf">Plasmid extraction</a>
</p>
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</p>
<h3>2. Plasmid Construction</h3>
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<h3>2. Plasmid Construction</h3>
<p>
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<p>
1) <a href="https://static.igem.org/mediawiki/2016/d/db/T--OUC-China--Gibson_aassembly.pdf">Gibson</a><br>
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1) <a href="https://static.igem.org/mediawiki/2016/d/db/T--OUC-China--Gibson_aassembly.pdf">Gibson</a><br>
2) <a href="https://static.igem.org/mediawiki/2016/6/6a/T--OUC-China--DNA_cassettes.pdf">DNA cassettes</a>
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2) <a href="https://static.igem.org/mediawiki/2016/6/6a/T--OUC-China--DNA_cassettes.pdf">DNA cassettes</a>
</p>
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</p>
<h3>3. Systems detection</h3>
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<h3>3. Systems detection</h3>
<p>
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<p>
1) <a href="https://static.igem.org/mediawiki/2016/b/b7/T--OUC-China--Total_RNA_Extraction.pdf">RNA extraction</a><br>
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1) <a href="https://static.igem.org/mediawiki/2016/b/b7/T--OUC-China--Total_RNA_Extraction.pdf">RNA extraction</a><br>
2) <a href="https://static.igem.org/mediawiki/2016/b/b9/T--OUC-China--Reserve_transcription.pdf">Reserve transcription</a><br>
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2) <a href="https://static.igem.org/mediawiki/2016/b/b9/T--OUC-China--Reserve_transcription.pdf">Reserve transcription</a><br>
3) <a href="https://static.igem.org/mediawiki/2016/7/7a/T--OUC-China--Quantitative_Real-time_PCR.pdf">Quantitative Real-time PCR</a><br>
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3) <a href="https://static.igem.org/mediawiki/2016/7/7a/T--OUC-China--Quantitative_Real-time_PCR.pdf">Quantitative Real-time PCR</a><br>
4) <a href="https://static.igem.org/mediawiki/2016/2/2f/T--OUC-China--Fluorescence_measurement.pdf">Fluorescence measurement protocol</a>
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4) <a href="https://static.igem.org/mediawiki/2016/2/2f/T--OUC-China--Fluorescence_measurement.pdf">Fluorescence measurement protocol</a>
</p>
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</p>
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Revision as of 12:11, 16 October 2016

Protocol