Difference between revisions of "Team:OUC-China/Protocol"
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<div class="container"> | <div class="container"> | ||
| − | <nav class="oucNav navbar navbar-default navbar-fixed-top" role="navigation"> | + | <nav class="oucNav navbar navbar-default navbar-fixed-top" role="navigation"> |
<div class="container"> | <div class="container"> | ||
<div class="navbar-header"> | <div class="navbar-header"> | ||
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<ul class="nav navbar-nav" id="oucNav"> | <ul class="nav navbar-nav" id="oucNav"> | ||
<li><a href="https://2016.igem.org/Team:OUC-China">Home</a></li> | <li><a href="https://2016.igem.org/Team:OUC-China">Home</a></li> | ||
| − | <li><a href="https://2016.igem.org/Team:OUC-China/Project"> | + | <li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Project<span class="caret"></span></a> |
| − | + | <ul class="dropdown-menu"> | |
| + | <li><a ref="https://2016.igem.org/Team:OUC-China/Project">Introduction</a></li> | ||
| + | <li><a href="https://2016.igem.org/Team:OUC-China/Design">Design</a></li> | ||
| + | <li><a href="https://2016.igem.org/Team:OUC-China/Proof">Proof of concept </a></li> | ||
| + | <li><a href="https://2016.igem.org/Team:OUC-China/Results">Results</a></li> | ||
| + | </ul> | ||
| + | </li> | ||
<li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Parts<span class="caret"></span></a> | <li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Parts<span class="caret"></span></a> | ||
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
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</ul> | </ul> | ||
</li> | </li> | ||
| − | + | <li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Human Practice<span class="caret"></span></a> | |
| − | <li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#"> | + | |
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
| − | <li><a href="https://2016.igem.org/Team:OUC-China/ | + | <li><a href="https://2016.igem.org/Team:OUC-China/Human_Practices">Overview</a></li> |
| − | + | <li><a href="https://2016.igem.org/Team:OUC-China/Communicating_&_improving">Communicating & improving</a></li> | |
| − | <li><a href="https://2016.igem.org/Team:OUC-China/ | + | <li><a href="https://2016.igem.org/Team:OUC-China/Investigating_&_promoting">Investigating & promoting</a></li> |
| + | <li><a href="https://2016.igem.org/Team:OUC-China/Potential_application">Potential application</a></li> | ||
</ul> | </ul> | ||
</li> | </li> | ||
| − | + | <li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Team<span class="caret"></span></a> | |
| − | <li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#"> | + | |
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
| − | <li><a href="https://2016.igem.org/Team:OUC-China/ | + | <li><a href="https://2016.igem.org/Team:OUC-China/Team">Team</a></li> |
<li><a href="https://2016.igem.org/Team:OUC-China/Collaborations">Collaborations</a></li> | <li><a href="https://2016.igem.org/Team:OUC-China/Collaborations">Collaborations</a></li> | ||
| + | </ul> | ||
| + | </li> | ||
| + | <li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Modeling<span class="caret"></span></a> | ||
| + | <ul class="dropdown-menu"> | ||
| + | <li><a href="https://2016.igem.org/Team:OUC-China/Model">Model</a></li> | ||
| + | <li><a href="https://2016.igem.org/Team:OUC-China/Software">Software</a></li> | ||
| + | </ul> | ||
| + | </li> | ||
| + | <li><a href="https://2016.igem.org/Team:OUC-China/Attributions">Attributions</a></li> | ||
| + | <li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Safety<span class="caret"></span></a> | ||
| + | <ul class="dropdown-menu"> | ||
| + | <li><a href="https://2016.igem.org/Team:OUC-China/Notebook">Notebook</a></li> | ||
| + | <li class="active"><a href="https://2016.igem.org/Team:OUC-China/Protocol">Protocol</a></li> | ||
| + | <li><a href="https://2016.igem.org/Team:OUC-China/Safety">Safety</a></li> | ||
</ul> | </ul> | ||
</li> | </li> | ||
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<div class="img_banner"><img src="https://static.igem.org/mediawiki/2016/f/fe/T--OUC-China--protocol-banner.jpg" class="img-responsive" alt="protocol-banner"></div> | <div class="img_banner"><img src="https://static.igem.org/mediawiki/2016/f/fe/T--OUC-China--protocol-banner.jpg" class="img-responsive" alt="protocol-banner"></div> | ||
<div style="background:#EFF2F6;"> | <div style="background:#EFF2F6;"> | ||
| − | + | <br> | |
| − | + | <br> | |
| − | + | <br> | |
| − | + | <br> | |
| − | + | <div class="row"> | |
| − | + | <div class="col-md-6 oucprotocol col-md-push-3" style="margin:0 auto;max-width:90%;border:2px solid #c0c0c0;border-radius:10px;background:#fff;"> | |
| − | + | <h3>1. Elementary Operation</h3> | |
| − | + | <p> | |
| − | + | 1) <a href="https://static.igem.org/mediawiki/2016/1/15/T--OUC-China--Luria-Bertani_medium.pdf">Luria-Bertani liquid and Luria-Bertani solid medium</a><br> | |
| − | + | 2) <a href="https://static.igem.org/mediawiki/2016/0/0c/T--OUC-China--Inoculation.pdf">Inoculation</a><br> | |
| − | + | 3) <a href="https://static.igem.org/mediawiki/2016/d/dc/T--OUC-China--Polymerase_Chain_Reaction.pdf">Polymerase Chain Reaction</a><br> | |
| − | + | 4) <a href="https://static.igem.org/mediawiki/2016/c/cb/T--OUC-China--Gel_Electrophoresis.pdf">Gel Electrophoresis</a><br> | |
| − | + | 5) <a href="https://static.igem.org/mediawiki/2016/0/00/T--OUC-China--Restriction_Enzyme_Digestion.pdf">Restriction Enzyme Digestion</a><br> | |
| − | + | 6) <a href="https://static.igem.org/mediawiki/2016/d/df/T--OUC-China--Gel_Extraction.pdf">Gel Extraction</a><br> | |
| − | + | 7) <a href="https://static.igem.org/mediawiki/2016/1/14/T--OUC-China--Ligation.pdf">Ligation system</a><br> | |
| − | + | 8) <a href="https://static.igem.org/mediawiki/2016/f/fb/T--OUC-China--Transformation_of_Escherichia_coli.pdf">Transformation of <i>E.coli</i></a><br> | |
| − | + | 9) <a href="https://static.igem.org/mediawiki/2016/8/84/T--OUC-China--Conservation.pdf">Conservation</a><br> | |
| − | + | 10) <a href="https://static.igem.org/mediawiki/2016/c/c8/T--OUC-China--Sterilization.pdf">Sterilization</a><br> | |
| − | + | 11) <a href="https://static.igem.org/mediawiki/2016/1/1f/T--OUC-China--Plasmid_extraction.pdf">Plasmid extraction</a> | |
| − | + | </p> | |
| − | + | <h3>2. Plasmid Construction</h3> | |
| − | + | <p> | |
| − | + | 1) <a href="https://static.igem.org/mediawiki/2016/d/db/T--OUC-China--Gibson_aassembly.pdf">Gibson</a><br> | |
| − | + | 2) <a href="https://static.igem.org/mediawiki/2016/6/6a/T--OUC-China--DNA_cassettes.pdf">DNA cassettes</a> | |
| − | + | </p> | |
| − | + | <h3>3. Systems detection</h3> | |
| − | + | <p> | |
| − | + | 1) <a href="https://static.igem.org/mediawiki/2016/b/b7/T--OUC-China--Total_RNA_Extraction.pdf">RNA extraction</a><br> | |
| − | + | 2) <a href="https://static.igem.org/mediawiki/2016/b/b9/T--OUC-China--Reserve_transcription.pdf">Reserve transcription</a><br> | |
| − | + | 3) <a href="https://static.igem.org/mediawiki/2016/7/7a/T--OUC-China--Quantitative_Real-time_PCR.pdf">Quantitative Real-time PCR</a><br> | |
| − | + | 4) <a href="https://static.igem.org/mediawiki/2016/2/2f/T--OUC-China--Fluorescence_measurement.pdf">Fluorescence measurement protocol</a> | |
| − | + | </p> | |
| − | + | </div> | |
| − | + | </div> | |
<br><br><br><br> | <br><br><br><br> | ||
</div> | </div> | ||
Revision as of 12:11, 16 October 2016
1. Elementary Operation
1) Luria-Bertani liquid and Luria-Bertani solid medium
2) Inoculation
3) Polymerase Chain Reaction
4) Gel Electrophoresis
5) Restriction Enzyme Digestion
6) Gel Extraction
7) Ligation system
8) Transformation of E.coli
9) Conservation
10) Sterilization
11) Plasmid extraction
2. Plasmid Construction
1) Gibson
2) DNA cassettes
3. Systems detection
1) RNA extraction
2) Reserve transcription
3) Quantitative Real-time PCR
4) Fluorescence measurement protocol
Cistrons Concerto
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