Difference between revisions of "Team:Oxford/Notebook"

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                           <li><a href="#16q5">Day 5</a></li>
 
                           <li><a href="#16q5">Day 5</a></li>
 
                           </ul>             
 
                           </ul>             
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                <a href="#Interlab">Interlab Notebook</a>
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                        <ul class="nav nav-stacked">
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                          </ul>                      </li>
 
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<h3>Day 1</h3>
 
<h3>Day 1</h3>
 
<p>
 
<p>
Preparation of Stock Solutions
 
 
• gBlocks
 
3 of our gBlocks from IDT had arrived: pCopA MymT HH (pc), MymT sfGFP HH (mg) and TAT Csp1 HH (tc). These arrived in the form of a solid, white powder.
 
 
We resuspended the gBlocks in different volumes of MilliQ, depending on the weight delivered, to give a stock solution of 10 ng/l.
 
 
Part name
 
Mass (ng)
 
Volume MilliQ added (l)
 
TAT Csp1 HH
 
500
 
50
 
MymT sfGFP HH
 
1000
 
100
 
pCopA MymT HH
 
1000
 
100
 
 
• Primers
 
The forward and reverse primers from IDT came as 32.4 nmol and 23.8 nmol of solid respectively.
 
 
Forward: 5’-CGACTTGATCACGTAGAATTC-3’
 
Reverse: 5’-ACACGATCGATATAACTGCAGC-3’
 
 
For our stock solutions, the primers were suspended in different volumes of MilliQ to give 100M.
 
 
Primer
 
Amount (nmol)
 
Volume MilliQ added (l)
 
Forward
 
32.4
 
324
 
Reverse
 
23.8
 
238
 
 
 
Preparation of reaction solutions
 
 
• gBlocks
 
Following resuspension, 10l of each DNA solution was added to 90l MilliQ (1:10 dilution) in an Eppendorf to give a final concentration of 1 ng/l and final solution volume of 100l.
 
 
• Primers
 
Following resuspension, 10l of each primer solution was added to 90l MilliQ (1:10 dilution) in an Eppendorf to give a final concentration of 10M and final solution volume of 100l.
 
 
</p>
 
</p>
 
</section>
 
</section>

Revision as of 17:02, 16 October 2016

iGEM Oxford 2016 - Cure for Copper

Notebook

Introduction

This page documents all the experiments we carried out in the wet lab as a part of our project. The details of the process we carried out can be found on our protocols page, and the chemicals we used can be found on the chemicals page.

Week 1

Day 1

Day 2

Day 3

Day 4

Day 5

Week 2

Day 1

Day 2

Day 3

Day 4

Day 5

Week 3

Day 1

Day 2

Day 3

Day 4

Day 5

Week 4

Day 1

Day 2

Day 3

Day 4

Day 5

Week 5

Day 1

Day 2

Day 3

Day 4

Day 5

Week 6

Day 1

Day 2

Day 3

Day 4

Day 5

Week 7

Day 1

Day 2

Day 3

Day 4

Day 5

Week 8

Day 1

Day 2

Day 3

Day 4

Day 5

Week 9

Day 1

Day 2

Day 3

Day 4

Day 5

Week 10

Day 1

Day 2

Day 3

Day 4

Day 5

Week 11

Day 1

Day 2

Day 3

Day 4

Day 5

Week 12

Day 1

Day 2

Day 3

Day 4

Day 5

Week 13

Day 1

Day 2

Day 3

Day 4

Day 5

Week 14

Day 1

Day 2

Day 3

Day 4

Day 5

Week 15

Day 1

Day 2

Day 3

Day 4

Day 5

Week 16

Day 1

Day 2

Day 3

Day 4

Day 5