Difference between revisions of "Team:Oxford/Chemicals"

Line 38: Line 38:
  
 
  <h2>Ethidium Bromide</h2>
 
  <h2>Ethidium Bromide</h2>
−
<div class="div50">
+
<div class="div50" style="padding-top: 50px;">
−
   <p>Use: Staining DNA electrophoresis gels.</p>
+
   <p><u>Use:</u> Staining DNA electrophoresis gels.</p>
−
   <p>Signal word Danger</p>
+
   <p><u>Signal word:</u> Danger</p>
 
</div>
 
</div>
 
<div class="div50">
 
<div class="div50">
Line 63: Line 63:
  
 
<h2>TBE Buffer pH 8.3</h2>
 
<h2>TBE Buffer pH 8.3</h2>
−
   <p>Use: General buffer for DNA experiments.<br>Made of three chemicals: Tris,borate and EDTA.</p>
+
   <p><u>Use:</u> General buffer for DNA experiments.<br>Made of three chemicals: Tris,borate and EDTA.</p>
 
   <p>Non-Hazardous</p>
 
   <p>Non-Hazardous</p>
 
  <img src="https://static.igem.org/mediawiki/2016/4/47/T--Oxford--TBE.gif" style="float: right" width="40%"/>
 
  <img src="https://static.igem.org/mediawiki/2016/4/47/T--Oxford--TBE.gif" style="float: right" width="40%"/>
Line 70: Line 70:
  
 
<h2>Copper Sulphate Pentahydrate:</h2>
 
<h2>Copper Sulphate Pentahydrate:</h2>
−
   <p>Use: Induction of copper promoters and Cu2+ chelation assays.</p>
+
   <p><u>Use:</u> Induction of copper promoters and Cu2+ chelation assays.</p>
−
   <p>Signal word: Warning</p>
+
   <p>u>Signal word:</u> Warning</p>
  
 
  <img src="https://static.igem.org/mediawiki/2016/b/b6/T--Oxford--CUSO4H2O.png" style="float: right" width="30%"/>
 
  <img src="https://static.igem.org/mediawiki/2016/b/b6/T--Oxford--CUSO4H2O.png" style="float: right" width="30%"/>
Line 91: Line 91:
  
 
  <h2>Agarose</h2>
 
  <h2>Agarose</h2>
−
   <p>Use: DNA electrophoresis gel matrix</p>
+
   <p><u>Use:</u> DNA electrophoresis gel matrix</p>
 
   <p>Non-Hazardous</p>
 
   <p>Non-Hazardous</p>
 
  <img src="https://static.igem.org/mediawiki/2016/d/df/T--Oxford--Agarose.png" style="float: right" width="40%"/>
 
  <img src="https://static.igem.org/mediawiki/2016/d/df/T--Oxford--Agarose.png" style="float: right" width="40%"/>
Line 98: Line 98:
  
 
<h2>L(+)-Arabinose</h2>
 
<h2>L(+)-Arabinose</h2>
−
<p>Use: Induction of pBAD expression</p>
+
<p><u>Use:</u> Induction of pBAD expression</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
  
Line 107: Line 107:
  
 
<h2>Bathocuproinedisulfonic Acid Disodium Salt</h2>
 
<h2>Bathocuproinedisulfonic Acid Disodium Salt</h2>
−
<p>Use: Chromophore in Cu+/Cu2+ detection assay</p>
+
<p><u>Use:</u> Chromophore in Cu+/Cu2+ detection assay</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
  
Line 115: Line 115:
  
 
<h2>L(+)-Sodium Ascorbate</h2>
 
<h2>L(+)-Sodium Ascorbate</h2>
−
<p>Use: Reducing agent in Cu+/Cu2+ detection assay</p>
+
<p><u>Use:</u> Reducing agent in Cu+/Cu2+ detection assay</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
  
Line 123: Line 123:
  
 
<h2>L-Glutathione</h2>
 
<h2>L-Glutathione</h2>
−
<p>Use: Reducing agent in Cu+/Cu2+ detection assay.</p>
+
<p><u>Use:</u> Reducing agent in Cu+/Cu2+ detection assay.</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
  
Line 131: Line 131:
  
 
<h2>Chloramphenicol</h2>
 
<h2>Chloramphenicol</h2>
−
<p>Use: Antibiotic and positive selection marker for all plasmids except pBAD</p>
+
<p><u>Use:</u> Antibiotic and positive selection marker for all plasmids except pBAD</p>
−
<p>Signal word: Danger</p>
+
<p>u>Signal word:</u> Danger</p>
  
 
<img src="https://static.igem.org/mediawiki/2016/c/cd/T--Oxford--Chloramphenicol.png" style="float: right" width="40%"/>
 
<img src="https://static.igem.org/mediawiki/2016/c/cd/T--Oxford--Chloramphenicol.png" style="float: right" width="40%"/>
Line 149: Line 149:
  
 
<h2>Ampicillin</h2>
 
<h2>Ampicillin</h2>
−
<p>Use: Antibiotic and positive selection marker for pBAD.</p>
+
<p><u>Use:</u> Antibiotic and positive selection marker for pBAD.</p>
−
<p>Signal word: Danger</p>
+
<p>u>Signal word:</u> Danger</p>
  
 
<img src="https://static.igem.org/mediawiki/2016/8/8f/T--Oxford--Ampicillin.png" style="float: right" width="40%"/>
 
<img src="https://static.igem.org/mediawiki/2016/8/8f/T--Oxford--Ampicillin.png" style="float: right" width="40%"/>
Line 173: Line 173:
  
 
<h2>Tris pH 7.0 and 8.0</h2>
 
<h2>Tris pH 7.0 and 8.0</h2>
−
<p>Use: General buffer for protein and copper assays</p>
+
<p><u>Use:</u> General buffer for protein and copper assays</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
  
Line 181: Line 181:
  
 
<h2>Glycerol</h2>
 
<h2>Glycerol</h2>
−
<p>Use: Component of protein buffer for protein stability at low temperatures. Used in protein purification lysis and elution buffers. Also, used in SDS-PAGE run buffer</p>
+
<p><u>Use:</u> Component of protein buffer for protein stability at low temperatures. Used in protein purification lysis and elution buffers. Also, used in SDS-PAGE run buffer</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
  
Line 189: Line 189:
  
 
<h2>DL-Dithiothreitol</h2>
 
<h2>DL-Dithiothreitol</h2>
−
<p>Use: Reducing agent in Cu+/Cu2+ detection assay and protein purification</p>
+
<p><u>Use:</u> Reducing agent in Cu+/Cu2+ detection assay and protein purification</p>
−
<p>Signal word: Warning</p>
+
<p>u>Signal word:</u> Warning</p>
  
 
<img src="https://static.igem.org/mediawiki/2016/e/ee/T--Oxford--DTT.png" style="float: right" width="40%"/>
 
<img src="https://static.igem.org/mediawiki/2016/e/ee/T--Oxford--DTT.png" style="float: right" width="40%"/>
Line 210: Line 210:
  
 
<h2>Imidazole</h2>
 
<h2>Imidazole</h2>
−
<p>Use: Elution of His-tagged target proteins from nickel bead column during protein purification</p>
+
<p><u>Use:</u> Elution of His-tagged target proteins from nickel bead column during protein purification</p>
−
<p>Signal word: Danger</p>
+
<p>u>Signal word:</u> Danger</p>
  
 
<img src="https://static.igem.org/mediawiki/2016/0/01/T--Oxford--Imidiazole.png" style="float: right" width="30%"/>
 
<img src="https://static.igem.org/mediawiki/2016/0/01/T--Oxford--Imidiazole.png" style="float: right" width="30%"/>
Line 233: Line 233:
  
 
<h2>Nickel Affinity Beads</h2>
 
<h2>Nickel Affinity Beads</h2>
−
<p>Use: Selection of His-tagged target proteins during protein purification</p>
+
<p><u>Use:</u> Selection of His-tagged target proteins during protein purification</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
  
Line 241: Line 241:
  
 
<h2>Sodium Alginate</h2>
 
<h2>Sodium Alginate</h2>
−
<p>Use: Major component of bacterial delivery beads</p>
+
<p><u>Use:</u> Major component of bacterial delivery beads</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
  
Line 249: Line 249:
  
 
<h2>Crystal Violet</h2>
 
<h2>Crystal Violet</h2>
−
<p>Use: Marker for bead quality assay</p>
+
<p><u>Use:</u> Marker for bead quality assay</p>
−
<p>Signal word: Danger</p>
+
<p>u>Signal word:</u> Danger</p>
  
 
<img src="https://static.igem.org/mediawiki/2016/f/f6/T--Oxford--Crystal_Violet.png" style="float: right" width="30%"/>
 
<img src="https://static.igem.org/mediawiki/2016/f/f6/T--Oxford--Crystal_Violet.png" style="float: right" width="30%"/>
Line 274: Line 274:
  
 
<h2>Lysogeny Broth</h2>
 
<h2>Lysogeny Broth</h2>
−
<p>Use: Media for general bacterial growth</p>
+
<p><u>Use:</u> Media for general bacterial growth</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
  
Line 282: Line 282:
  
 
<h2>Phosphate Buffered Saline pH 7.4</h2>
 
<h2>Phosphate Buffered Saline pH 7.4</h2>
−
<p>Use: Dilution buffer for flow cytometry experiments</p>
+
<p><u>Use:</u> Dilution buffer for flow cytometry experiments</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
  
Line 290: Line 290:
  
 
<h2>Sodium Dodecyl Sulphate</h2>
 
<h2>Sodium Dodecyl Sulphate</h2>
−
<p>Use: Detergent in protein identification gels</p>
+
<p><u>Use:</u> Detergent in protein identification gels</p>
−
<p>Signal word: Danger</p>
+
<p>u>Signal word:</u> Danger</p>
  
 
<img src="https://static.igem.org/mediawiki/2016/1/15/T--Oxford--SDS.jpg" class="rotate" style="float: right" width="50%"/>
 
<img src="https://static.igem.org/mediawiki/2016/1/15/T--Oxford--SDS.jpg" class="rotate" style="float: right" width="50%"/>
Line 317: Line 317:
  
 
<h2>Ethanol 100%</h2>
 
<h2>Ethanol 100%</h2>
−
<p>Use: solvent for chloramphenicol</p>
+
<p><u>Use:</u> solvent for chloramphenicol</p>
−
<p>Signal word: Danger</p>
+
<p>u>Signal word:</u> Danger</p>
  
 
<img src="https://static.igem.org/mediawiki/2016/b/b2/T--Oxford--Ethanol.png" style="float: right" width="60%"/>
 
<img src="https://static.igem.org/mediawiki/2016/b/b2/T--Oxford--Ethanol.png" style="float: right" width="60%"/>
Line 338: Line 338:
  
 
<h2>Transfection Buffer I (Manganese[II] Chloride)</h2>
 
<h2>Transfection Buffer I (Manganese[II] Chloride)</h2>
−
<p>Use: First soaking buffer in creating competent E. coli cells</p>
+
<p><u>Use:</u> First soaking buffer in creating competent E. coli cells</p>
−
<p>Signal word: Danger</p>
+
<p>u>Signal word:</u> Danger</p>
  
 
<img src="https://static.igem.org/mediawiki/2016/f/fa/T--Oxford--MnCl.png" style="float: right"/>
 
<img src="https://static.igem.org/mediawiki/2016/f/fa/T--Oxford--MnCl.png" style="float: right"/>
Line 363: Line 363:
  
 
<h2>Transfection Buffer II</h2>
 
<h2>Transfection Buffer II</h2>
−
<p>Use: Second soaking buffer in creating competent E. coli cells</p>
+
<p><u>Use:</u> Second soaking buffer in creating competent E. coli cells</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
  
Line 369: Line 369:
  
 
<h2>Chitosan</h2>
 
<h2>Chitosan</h2>
−
<p>Use: Coating for bacterial delivery beads</p>
+
<p><u>Use:</u> Coating for bacterial delivery beads</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
  
Line 377: Line 377:
  
 
<h2>InstantBlue</h2>
 
<h2>InstantBlue</h2>
−
<p>Use: Protein stain for protein identification gels</p>
+
<p><u>Use:</u> Protein stain for protein identification gels</p>
−
<p>Signal word: Warning</p>
+
<p>u>Signal word:</u> Warning</p>
  
 
<img src="https://static.igem.org/mediawiki/2016/9/9c/T--Oxford--InstantBlue.png" style="float: right" width="50%"/>
 
<img src="https://static.igem.org/mediawiki/2016/9/9c/T--Oxford--InstantBlue.png" style="float: right" width="50%"/>
Line 396: Line 396:
  
 
<h2>NEB Purple Loading Dye</h2>
 
<h2>NEB Purple Loading Dye</h2>
−
<p>Use: Stabilizer and marker for DNA gel electrophoresis</p>
+
<p><u>Use:</u> Stabilizer and marker for DNA gel electrophoresis</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
  
Line 404: Line 404:
  
 
<h2>Roche cOmplete EDTA-free Protease Inhibitor Cocktail</h2>
 
<h2>Roche cOmplete EDTA-free Protease Inhibitor Cocktail</h2>
−
<p>Use: Prevention of protein cleavage during protein purification</p>
+
<p><u>Use:</u> Prevention of protein cleavage during protein purification</p>
−
<p>Signal word Danger</p>
+
<p>u>Signal word:</u> Danger</p>
  
 
<img src="https://static.igem.org/mediawiki/2016/6/68/T--Oxford--protease_inhibitors.jpg" style="float: right"/>
 
<img src="https://static.igem.org/mediawiki/2016/6/68/T--Oxford--protease_inhibitors.jpg" style="float: right"/>
Line 426: Line 426:
 
<section>
 
<section>
 
<h2>Hydrochloric Acid 1M</h2>
 
<h2>Hydrochloric Acid 1M</h2>
−
<p>Use: Simulation of stomach acid conditions in bead experiments</p>
+
<p><u>Use:</u> Simulation of stomach acid conditions in bead experiments</p>
−
<p>Signal word: Warning</p>
+
<p>u>Signal word:</u> Warning</p>
  
 
<img src="https://static.igem.org/mediawiki/2016/f/f5/T--Oxford--HCL.png" style="float: right" width="40%"/>
 
<img src="https://static.igem.org/mediawiki/2016/f/f5/T--Oxford--HCL.png" style="float: right" width="40%"/>
Line 441: Line 441:
  
 
<h2>Sodium Hydroxide</h2>
 
<h2>Sodium Hydroxide</h2>
−
<p>Use: Simulation of duodenum conditions in bead experiments</p>
+
<p><u>Use:</u> Simulation of duodenum conditions in bead experiments</p>
−
<p>Signal word: Danger</p>
+
<p>u>Signal word:</u> Danger</p>
  
 
<img src="https://static.igem.org/mediawiki/2016/d/d7/T--Oxford--SODIUM_HYDROXIDE.png" style="float: right" width="40%"/>
 
<img src="https://static.igem.org/mediawiki/2016/d/d7/T--Oxford--SODIUM_HYDROXIDE.png" style="float: right" width="40%"/>
Line 464: Line 464:
  
 
<h2>Monobasic Sodium Phosphate</h2>
 
<h2>Monobasic Sodium Phosphate</h2>
−
<p>Use: Simulation of duodenum conditions in bead experiments</p>
+
<p><u>Use:</u> Simulation of duodenum conditions in bead experiments</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
  
Line 473: Line 473:
  
 
<h2>Glacial Acetic Acid</h2>
 
<h2>Glacial Acetic Acid</h2>
−
<p>Use: Bead experiments</p>
+
<p><u>Use:</u> Bead experiments</p>
−
<p>Signal word: Danger</p>
+
<p>u>Signal word:</u> Danger</p>
  
 
<img src="https://static.igem.org/mediawiki/2016/1/16/T--Oxford--Acetic_Acid.png" style="float: right" width="40%"/>
 
<img src="https://static.igem.org/mediawiki/2016/1/16/T--Oxford--Acetic_Acid.png" style="float: right" width="40%"/>
Line 496: Line 496:
  
 
<h2>Calcium Chloride</h2>
 
<h2>Calcium Chloride</h2>
−
<p>Use: Forming beads from alginate.  Ca2+ causes alginate phase change</p>
+
<p><u>Use:</u> Forming beads from alginate.  Ca2+ causes alginate phase change</p>
−
<p>Signal word Warning</p>
+
<p>u>Signal word:</u> Warning</p>
  
 
<img src="https://static.igem.org/mediawiki/2016/8/85/T--Oxford--CACL2.png" style="float: right"/>
 
<img src="https://static.igem.org/mediawiki/2016/8/85/T--Oxford--CACL2.png" style="float: right"/>

Revision as of 20:00, 16 October 2016

iGEM Oxford 2016 - Cure for Copper

Chemicals

Ethidium Bromide

Use: Staining DNA electrophoresis gels.

Signal word: Danger


Hazard statement(s)

  • H302 Harmful if swallowed.
  • H330 Fatal if inhaled.
  • H341 Suspected of causing genetic defects.

Precautionary statement(s)

  • P260 Do not breathe dust/fume/gas/mist/vapours/spray.
  • P281 Use personal protective equipment as required.
  • P284 Wear respiratory protection.
  • P310 Immediately call a POISON CENTER or doctor/ physician.

TBE Buffer pH 8.3

Use: General buffer for DNA experiments.
Made of three chemicals: Tris,borate and EDTA.

Non-Hazardous

Copper Sulphate Pentahydrate:

Use: Induction of copper promoters and Cu2+ chelation assays.

u>Signal word: Warning

Hazard statement(s)

  • H302 Harmful if swallowed.
  • H315 Causes skin irritation.
  • H319 Causes serious eye irritation.
  • H410 Very toxic to aquatic life with long lasting effects.

Precautionary statement(s)

  • P273 Avoid release to the environment.
  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes.Remove contact lenses, if present and easy to do. Continue rinsing.
  • P501 Dispose of contents/container to an approved waste disposal plant.

Agarose

Use: DNA electrophoresis gel matrix

Non-Hazardous

L(+)-Arabinose

Use: Induction of pBAD expression

Non-Hazardous

Bathocuproinedisulfonic Acid Disodium Salt

Use: Chromophore in Cu+/Cu2+ detection assay

Non-Hazardous

L(+)-Sodium Ascorbate

Use: Reducing agent in Cu+/Cu2+ detection assay

Non-Hazardous

L-Glutathione

Use: Reducing agent in Cu+/Cu2+ detection assay.

Non-Hazardous

Chloramphenicol

Use: Antibiotic and positive selection marker for all plasmids except pBAD

u>Signal word: Danger

Hazard statement(s)

  • H350 May cause cancer

Precautionary statement(s)

  • P201 Obtain special instructions before use.
  • P308 + P313 If exposed or concerned: Get medical advice/attention.

Ampicillin

Use: Antibiotic and positive selection marker for pBAD.

u>Signal word: Danger

Hazard statement(s)

  • H315 Causes skin irritation
  • H317 May cause an allergic skin reaction
  • H319 Causes serious eye irritation
  • H334 May cause allergy or asthma symptoms or breathing difficulties if inhaled
  • H335 May cause respiratory irritation

Precautionary statement(s)

  • P261 Avoid breathing dust/fume/gas/mist/vapours/spray
  • P280 Wear protective gloves
  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
  • P342 + P311 If experiencing respiratory symptoms: Call a POISON CENTER or doctor/physician

Tris pH 7.0 and 8.0

Use: General buffer for protein and copper assays

Non-Hazardous

Glycerol

Use: Component of protein buffer for protein stability at low temperatures. Used in protein purification lysis and elution buffers. Also, used in SDS-PAGE run buffer

Non-Hazardous

DL-Dithiothreitol

Use: Reducing agent in Cu+/Cu2+ detection assay and protein purification

u>Signal word: Warning

Hazard statement(s)

  • H302 Harmful if swallowed
  • H315 Causes skin irritation
  • H319 Causes serious eye irritation
  • H335 May cause respiratory irritation

Precautionary statement(s)

  • P261 Avoid breathing dust
  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing

Imidazole

Use: Elution of His-tagged target proteins from nickel bead column during protein purification

u>Signal word: Danger

Hazard statement(s)

  • H302 Harmful if swallowed
  • H314 Causes severe skin burns and eye damage
  • H360D May damage the unborn child

Precautionary statement(s)

  • P201 Obtain special instructions before use
  • P280 Wear protective gloves/protective clothing/eye protection/face protection
  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
  • P310 Immediately call a POISON CENTER or doctor/physician

Nickel Affinity Beads

Use: Selection of His-tagged target proteins during protein purification

Non-Hazardous

Sodium Alginate

Use: Major component of bacterial delivery beads

Non-Hazardous

Crystal Violet

Use: Marker for bead quality assay

u>Signal word: Danger

Hazard statement(s)

  • H302 Harmful if swallowed
  • H318 Causes serious eye damage
  • H350 May cause cancer
  • H410 Very toxic to aquatic life with long lasting effects

Precautionary statement(s)

  • P201 Obtain special instructions before use
  • P273 Avoid release to the environment
  • P280 Wear protective gloves/ eye protection/ face protection
  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
  • P308 + P313 If exposed or concerned: Get medical advice/attention
  • P501 Dispose of contents/container to an approved waste disposal plant

Lysogeny Broth

Use: Media for general bacterial growth

Non-Hazardous

Phosphate Buffered Saline pH 7.4

Use: Dilution buffer for flow cytometry experiments

Non-Hazardous

Sodium Dodecyl Sulphate

Use: Detergent in protein identification gels

u>Signal word: Danger

Hazard statement(s)

  • H228 Flammable solid
  • H302 + H332 Harmful if swallowed or if inhaled
  • H315 Causes skin irritation
  • H318 Causes serious eye damage
  • H335 May cause respiratory irritation
  • H412 Harmful to aquatic life with long lasting effects

Precautionary statement(s)

  • P210 Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking
  • P261 Avoid breathing dust/fume/gas/mist/vapours/spray
  • P280 Wear eye protection/face protection
  • P301 + P312 + P330 IF SWALLOWED: Call a POISON CENTER/doctor if you feel unwell. Rinse mouth
  • P305 + P351 + P338 + P310 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER/doctor
  • P370 + P378 In case of fire: Use dry powder or dry sand to extinguish

Ethanol 100%

Use: solvent for chloramphenicol

u>Signal word: Danger

Hazard statement(s)

  • H225 Highly flammable liquid and vapour
  • H319 Causes serious eye irritation

Precautionary statement(s)

  • P210 Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking
  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
  • P370 + P378 In case of fire: Use dry powder or dry sand to extinguish
  • P403 + P235 Store in a well-ventilated place. Keep cool

Transfection Buffer I (Manganese[II] Chloride)

Use: First soaking buffer in creating competent E. coli cells

u>Signal word: Danger

Hazard statement(s)

  • H302 Harmful if swallowed
  • H318 Causes serious eye damage
  • H373 May cause damage to organs (Brain) through prolonged or repeated exposure if inhaled
  • H411 Toxic to aquatic life with long lasting effects

Precautionary statement(s)

  • P273 Avoid release to the environment
  • P280 Wear eye protection/ face protection
  • P301 + P312 + P330 IF SWALLOWED: Call a POISON CENTER or doctor/physician if you feel unwell. Rinse mouth
  • P305 + P351 + P338 + P310 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER or doctor/physician
  • P391 Collect spillage
  • P501 Dispose of contents/container to an approved waste disposal plant

Transfection Buffer II

Use: Second soaking buffer in creating competent E. coli cells

Non-Hazardous

Chitosan

Use: Coating for bacterial delivery beads

Non-Hazardous

InstantBlue

Use: Protein stain for protein identification gels

u>Signal word: Warning

Hazard statement(s)

  • H290 May be corrosive to metals
  • H315 Causes skin irritation
  • H319 Causes serious eye irritation

Precautionary statement(s)

  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing

NEB Purple Loading Dye

Use: Stabilizer and marker for DNA gel electrophoresis

Non-Hazardous

Roche cOmplete EDTA-free Protease Inhibitor Cocktail

Use: Prevention of protein cleavage during protein purification

u>Signal word: Danger

Hazard statement(s)

  • H314 Causes severe skin burns and eye damage Skin corrosion/irritation

Precautionary statement(s)

  • P260 Do not breathe dust/fume/gas/mist/vapours/spray
  • P280 Wear protective gloves/protective clothing/eye protection/face protection
  • P301 + P330 + P331 IF SWALLOWED: Rinse mouth. Do NOT induce vomiting
  • P303 + P361 + P353 IF ON SKIN (or hair): Remove/Take off Immediately all contaminated clothing. Rinse SKIN with water/shower
  • P304 + P340 + P310 IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing. Immediately call a POISON CENTER or doctor/physician
  • P305 + P351 + P338 + P310 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER or doctor/physician

Hydrochloric Acid 1M

Use: Simulation of stomach acid conditions in bead experiments

u>Signal word: Warning

Hazard statement(s)

  • H290 May be corrosive to metals

Sodium Hydroxide

Use: Simulation of duodenum conditions in bead experiments

u>Signal word: Danger

Hazard statement(s)

  • H290 May be corrosive to metals
  • H314 Causes severe skin burns and eye damage

Precautionary statement(s)

  • P260 Do not breathe dust/fume/gas/mist/vapours/spray
  • P280 Wear protective gloves/protective clothing/eye protection/face protection
  • P303 + P361 + P353 IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/shower
  • P304 + P340 + P310 IF INHALED: Remove person to fresh air and keep comfortable for breathing. Immediately call a POISON CENTER/doctor
  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing

Monobasic Sodium Phosphate

Use: Simulation of duodenum conditions in bead experiments

Non-Hazardous

Glacial Acetic Acid

Use: Bead experiments

u>Signal word: Danger

Hazard statement(s)

  • H226 Flammable liquid and vapour
  • H314 Causes severe skin burns and eye damage

Precautionary statement(s)

  • P210 Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking
  • P260 Do not breathe dust/fume/gas/mist/vapours/spray
  • P280 Wear protective gloves/protective clothing/eye protection/face protection
  • P303 + P361 + P353 IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/shower
  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
  • P370 + P378 In case of fire: Use dry powder or dry sand to extinguish

Calcium Chloride

Use: Forming beads from alginate. Ca2+ causes alginate phase change

u>Signal word: Warning

Hazard statement(s)

  • H319 Causes serious eye irritation

Precautionary statement(s)

  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing