Difference between revisions of "Team:Manchester/Model/Costing"

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<p id = "OverviewTitle" style="border-bottom: 1px black solid ;font-size:25px;text-weight:bold;display:inline-block">Overview and Motivation</p>
 
<p id = "OverviewTitle" style="border-bottom: 1px black solid ;font-size:25px;text-weight:bold;display:inline-block">Overview and Motivation</p>
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<p> During our human practices discussion with the <a href="https://2016.igem.org/Team:Manchester/Human_Practices/Professional_Bodies#gmp">Police</a> it was brought to our attention that not all officers carry a breathalyser device and they are rather bulky: our patch could be an alternative, compact solution that all officers could carry provided it was suitable. </p>
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During our human practices discussion with the <a href="https://2016.igem.org/Team:Manchester/Human_Practices/Professional_Bodies#gmp">Police</a> it was brought to our attention that not all officers carry a breathalyser device and they are rather bulky: our patch could be an alternative, compact solution that all officers could carry provided it was suitable.
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<p> This obviously introduces constraints to the device if is to be suitable for this purpose. </p>
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This obviously introduces constraints to the device if is to be suitable for this purpose.
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<p> While this analysis is regarding Glucose not Alcohol it is included as a proof of concept of the system rather than to provide informative results. Since the majority of the project focused on Alcohol however these constraints were still used as all analyses should be informed and guided by the human practices. </p>
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While this analysis is regarding Glucose not Alcohol it is included as a proof of concept of the system rather than to provide informative results. Since the majority of the project focused on Alcohol however these constraints were still used as all analyses should be informed and guided by the human practices.
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<p id = "MethodologyTitle" style="border-bottom: 1px black solid ;font-size:25px;text-weight:bold;display:inline-block">Methodology</p>
 
<p id = "MethodologyTitle" style="border-bottom: 1px black solid ;font-size:25px;text-weight:bold;display:inline-block">Methodology</p>
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<p> Time independant analysis </p>
 
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The model was run for a range of different enzyme ratios, maitaining a constant total amount of enzyme
 
The model was run for a range of different enzyme ratios, maitaining a constant total amount of enzyme
 
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<p> MATHJAX CONCERVATION EQUATION ([Etotal] = [GOx] + [HRP] = constant) </p>
 
<p> MATHJAX CONCERVATION EQUATION ([Etotal] = [GOx] + [HRP] = constant) </p>
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All simulations that didn't violate the constraints were recorded
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<p> All simulations that didn't violate the constraints were recorded</p>
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<p>MATHJAX CONSTRAINT 1 ([Oxidised ABTS @ tmax] > [Minimum Oxidised ABTS]) </p>
 
<p>MATHJAX CONSTRAINT 1 ([Oxidised ABTS @ tmax] > [Minimum Oxidised ABTS]) </p>
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The cost of the simulation was estimated assuming the cost of everything but the enzyme costs are negligible
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<p>The cost of the simulation was estimated assuming the cost of everything but the enzyme costs are negligible</p>
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<p>MATHJAX COST EQUATION (CostTotal = CostGOx * [GOx] + CostHRP * [HRP])</p>
 
<p>MATHJAX COST EQUATION (CostTotal = CostGOx * [GOx] + CostHRP * [HRP])</p>
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<p> Time dependant analysis </p>
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<p id = "ResultsTitle" style="border-bottom: 1px black solid ;font-size:25px;text-weight:bold;display:inline-block">Results</p>
 
<p id = "ResultsTitle" style="border-bottom: 1px black solid ;font-size:25px;text-weight:bold;display:inline-block">Results</p>

Revision as of 20:40, 16 October 2016

Manchester iGEM 2016

Cost Analysis


Contents

Overview and Motivation
Methodology
Results
Conclusions

Overview and Motivation

During our human practices discussion with the Police it was brought to our attention that not all officers carry a breathalyser device and they are rather bulky: our patch could be an alternative, compact solution that all officers could carry provided it was suitable.

This obviously introduces constraints to the device if is to be suitable for this purpose.

Constraint Summary Constraint Reasoning
Maximum expression time The AlcoPatch would need to equally as fast as current methods, if not faster. This would increase the likelihood of uptake as it is an improvement on the current portable methods of blood alcohol detection Requirement suggested during the discussion
Minimum expression amount Expression needs to be high enough that the result can be seen since the device it designed to be portable and used 'roadside' where lighting conditions may not be ideal Experimental observation

While this analysis is regarding Glucose not Alcohol it is included as a proof of concept of the system rather than to provide informative results. Since the majority of the project focused on Alcohol however these constraints were still used as all analyses should be informed and guided by the human practices.


Methodology

Time independant analysis

The model was run for a range of different enzyme ratios, maitaining a constant total amount of enzyme

MATHJAX CONCERVATION EQUATION ([Etotal] = [GOx] + [HRP] = constant)

All simulations that didn't violate the constraints were recorded

MATHJAX CONSTRAINT 1 ([Oxidised ABTS @ tmax] > [Minimum Oxidised ABTS])

The cost of the simulation was estimated assuming the cost of everything but the enzyme costs are negligible

MATHJAX COST EQUATION (CostTotal = CostGOx * [GOx] + CostHRP * [HRP])


Time dependant analysis


Results

PLACEHOLDER


Conclusions

PLACEHOLDER



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