Difference between revisions of "Team:Oxford/Chemicals"

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<nav class="col-md-3 bs-docs-sidebar navFont">
 
<nav class="col-md-3 bs-docs-sidebar navFont">
 
         <ul id="sidebar" class="nav nav-stacked" data-spy="affix" data-offset-top="330">
 
         <ul id="sidebar" class="nav nav-stacked" data-spy="affix" data-offset-top="330">
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             <li>
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             <li><a href="#Cloning">Chemicals</a>
−
                 <a href="#1">1</a>
+
                 <ul class="nav nav-stacked">
 +
                    <li><a href="#Ethidium-Bromide">Ethidium Bromide</a></li>
 +
                    <li><a href="#TBE-Buffer">TBE Buffer</a></li>
 +
                    <li><a href="#Copper-Sulfate-Pentahydrate">Copper Sulphate Pentahydrate</a></li>
 +
                    <li><a href="#Agarose">Agarose</a></li>
 +
                    <li><a href="#L-Arabionse">L-Arabionse</a></li>
 +
                    <li><a href="#BCA-Salt">BCA Salt</a></li>
 +
                    <li><a href="#Sodium-Ascorbate">Sodium Ascorbate</a></li>
 +
                    <li><a href="#L-Glutathione">L-Glutathione</a></li>
 +
                    <li><a href="#Chloramphenicol">Chloramphenicol</a></li>
 +
                    <li><a href="#Ampicillin">Ampicillin</a></li>
 +
                    <li><a href="#Tris">Tris</a></li>
 +
                    <li><a href="#Glycerol">Glycerol</a></li>
 +
                    <li><a href="#DL-Dithiothreitol">D/L-Dithiothreitol</a></li>
 +
                    <li><a href="#Imidiazole">Imidiazole</a></li>
 +
                    <li><a href="#Nickel-Affinity-Beads">Nickel Affinity Beads</a></li>
 +
                    <li><a href="#Sodium-Alginate">Sodium Alginate</a></li>
 +
                    <li><a href="#Crystal-Violet">Crystal Violet</a></li>
 +
                    <li><a href="#Lysogeny-Broth">Lysogeny Broth</a></li>
 +
                    <li><a href="#Phosphate-Buffered-Saline">Phosphate Buffered Saline</a></li>
 +
                    <li><a href="#Sodium-Dodecyl-Sulfate">Sodium Dodecyl Sulphate</a></li>
 +
                    <li><a href="#Ethanol">Ethanol</a></li>
 +
                    <li><a href="#Transfection-Buffer-I">Transfection Buffer I</a></li>
 +
                    <li><a href="#Transfection-Buffer-II">Transfection Buffer II </a></li>
 +
                    <li><a href="#Chitosan">Chitosan</a></li>
 +
                    <li><a href="#Instant-Blue">Instant Blue</a></li>
 +
                    <li><a href="#NCB-Purple-Loading-Dye">NCB Purple Loading Dye</a></li>
 +
                    <li><a href="#Protease-Inhibitors">Protease Inhibitors</a></li>
 +
                    <li><a href="#Hydrochloric-Acid">Hydrochloric Acid</a></li>
 +
                    <li><a href="#Sodium-Hydroxide">Sodium Hydroxide</a></li>
 +
                    <li><a href="#Monobasic-Sodium-Phosphate">Monobasic Sodium Phosphate</a></li>
 +
                    <li><a href="#Glacial-Acetic-Acid">Glacial Acetic Acid</a></li>
 +
                    <li><a href="#Calcium-Chloride">Calcium Chloride</a></li>
 +
                </ul>     
 
             </li>
 
             </li>
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            <li>
+
</nav>
−
                <a href="#2">2</a>
+
 
−
            </li>
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−
            <li>
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−
                <a href="#3">3</a>
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−
            </li>
+
−
        </ul>
+
−
    </nav>
+
  
  
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<!-- Ethidium Bromide -->
 
<!-- Ethidium Bromide -->
  
−
  <h2>Ethidium Bromide</h2>
+
  <h2 id="Ethidium-Bromide">Ethidium Bromide</h2>
 
<div class="div50" style="padding-top: 50px;">
 
<div class="div50" style="padding-top: 50px;">
 
   <p><u>Use:</u> Staining DNA electrophoresis gels.</p>
 
   <p><u>Use:</u> Staining DNA electrophoresis gels.</p>
Line 62: Line 89:
 
<!-- TBE -->
 
<!-- TBE -->
  
−
<h2>TBE Buffer pH 8.3</h2>
+
<h2 id="TBE-Buffer">TBE Buffer pH 8.3</h2>
 
   <p><u>Use:</u> General buffer for DNA experiments.<br>Made of three chemicals: Tris,borate and EDTA.</p>
 
   <p><u>Use:</u> General buffer for DNA experiments.<br>Made of three chemicals: Tris,borate and EDTA.</p>
 
   <p>Non-Hazardous</p>
 
   <p>Non-Hazardous</p>
Line 69: Line 96:
 
<!-- Copper Sulphate -->
 
<!-- Copper Sulphate -->
  
−
<h2>Copper Sulphate Pentahydrate:</h2>
+
<h2 id="Copper-Sulfate-Pentahydrate">Copper Sulphate Pentahydrate:</h2>
 
   <p><u>Use:</u> Induction of copper promoters and Cu2+ chelation assays.</p>
 
   <p><u>Use:</u> Induction of copper promoters and Cu2+ chelation assays.</p>
 
   <p>u>Signal word:</u> Warning</p>
 
   <p>u>Signal word:</u> Warning</p>
Line 90: Line 117:
 
<!-- agarose -->
 
<!-- agarose -->
  
−
  <h2>Agarose</h2>
+
  <h2 id="Agarose">Agarose</h2>
 
   <p><u>Use:</u> DNA electrophoresis gel matrix</p>
 
   <p><u>Use:</u> DNA electrophoresis gel matrix</p>
 
   <p>Non-Hazardous</p>
 
   <p>Non-Hazardous</p>
Line 97: Line 124:
 
<!-- Arabinose -->
 
<!-- Arabinose -->
  
−
<h2>L(+)-Arabinose</h2>
+
<h2 id="L-Arabinose">L(+)-Arabinose</h2>
 
<p><u>Use:</u> Induction of pBAD expression</p>
 
<p><u>Use:</u> Induction of pBAD expression</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
Line 106: Line 133:
 
<!-- Bathocuproinedisulfonic Acid Disodium salt -->
 
<!-- Bathocuproinedisulfonic Acid Disodium salt -->
  
−
<h2>Bathocuproinedisulfonic Acid Disodium Salt</h2>
+
<h2 id="BCS-Salt">Bathocuproinedisulfonic Acid Disodium Salt</h2>
 
<p><u>Use:</u> Chromophore in Cu+/Cu2+ detection assay</p>
 
<p><u>Use:</u> Chromophore in Cu+/Cu2+ detection assay</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
Line 114: Line 141:
 
<!-- Ascorbate -->
 
<!-- Ascorbate -->
  
−
<h2>L(+)-Sodium Ascorbate</h2>
+
<h2 id="Sodium-Ascorbate">L(+)-Sodium Ascorbate</h2>
 
<p><u>Use:</u> Reducing agent in Cu+/Cu2+ detection assay</p>
 
<p><u>Use:</u> Reducing agent in Cu+/Cu2+ detection assay</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
Line 122: Line 149:
 
<!-- Glutathione -->
 
<!-- Glutathione -->
  
−
<h2>L-Glutathione</h2>
+
<h2 id="L-Glutathione">L-Glutathione</h2>
 
<p><u>Use:</u> Reducing agent in Cu+/Cu2+ detection assay.</p>
 
<p><u>Use:</u> Reducing agent in Cu+/Cu2+ detection assay.</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
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<!-- Chloramphenicol -->
 
<!-- Chloramphenicol -->
  
−
<h2>Chloramphenicol</h2>
+
<h2 id="Chloramphenicol">Chloramphenicol</h2>
 
<p><u>Use:</u> Antibiotic and positive selection marker for all plasmids except pBAD</p>
 
<p><u>Use:</u> Antibiotic and positive selection marker for all plasmids except pBAD</p>
 
<p>u>Signal word:</u> Danger</p>
 
<p>u>Signal word:</u> Danger</p>
Line 148: Line 175:
 
<!-- Ampicillin -->
 
<!-- Ampicillin -->
  
−
<h2>Ampicillin</h2>
+
<h2 id="Ampicillin">Ampicillin</h2>
 
<p><u>Use:</u> Antibiotic and positive selection marker for pBAD.</p>
 
<p><u>Use:</u> Antibiotic and positive selection marker for pBAD.</p>
 
<p>u>Signal word:</u> Danger</p>
 
<p>u>Signal word:</u> Danger</p>
Line 172: Line 199:
 
<!-- Tris -->
 
<!-- Tris -->
  
−
<h2>Tris pH 7.0 and 8.0</h2>
+
<h2 id="Tris">Tris pH 7.0 and 8.0</h2>
 
<p><u>Use:</u> General buffer for protein and copper assays</p>
 
<p><u>Use:</u> General buffer for protein and copper assays</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
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<!-- Glycerol -->
 
<!-- Glycerol -->
  
−
<h2>Glycerol</h2>
+
<h2 id="Glycerol">Glycerol</h2>
 
<p><u>Use:</u> Component of protein buffer for protein stability at low temperatures. Used in protein purification lysis and elution buffers. Also, used in SDS-PAGE run buffer</p>
 
<p><u>Use:</u> Component of protein buffer for protein stability at low temperatures. Used in protein purification lysis and elution buffers. Also, used in SDS-PAGE run buffer</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
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<!-- DTT -->
 
<!-- DTT -->
  
−
<h2>DL-Dithiothreitol</h2>
+
<h2 id="DL-Dithiothreitol">DL-Dithiothreitol</h2>
 
<p><u>Use:</u> Reducing agent in Cu+/Cu2+ detection assay and protein purification</p>
 
<p><u>Use:</u> Reducing agent in Cu+/Cu2+ detection assay and protein purification</p>
 
<p>u>Signal word:</u> Warning</p>
 
<p>u>Signal word:</u> Warning</p>
Line 209: Line 236:
 
<!-- Imidiazole -->
 
<!-- Imidiazole -->
  
−
<h2>Imidazole</h2>
+
<h2 id="Imidiazole">Imidazole</h2>
 
<p><u>Use:</u> Elution of His-tagged target proteins from nickel bead column during protein purification</p>
 
<p><u>Use:</u> Elution of His-tagged target proteins from nickel bead column during protein purification</p>
 
<p>u>Signal word:</u> Danger</p>
 
<p>u>Signal word:</u> Danger</p>
Line 232: Line 259:
 
<!-- Nickel affinity beads -->
 
<!-- Nickel affinity beads -->
  
−
<h2>Nickel Affinity Beads</h2>
+
<h2 id="Nickel-Affinity-Beads">Nickel Affinity Beads</h2>
 
<p><u>Use:</u> Selection of His-tagged target proteins during protein purification</p>
 
<p><u>Use:</u> Selection of His-tagged target proteins during protein purification</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
Line 240: Line 267:
 
<!-- Sodium Alginate -->
 
<!-- Sodium Alginate -->
  
−
<h2>Sodium Alginate</h2>
+
<h2 id="Sodium-Alginate">Sodium Alginate</h2>
 
<p><u>Use:</u> Major component of bacterial delivery beads</p>
 
<p><u>Use:</u> Major component of bacterial delivery beads</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
Line 248: Line 275:
 
<!-- Crystal Violet -->
 
<!-- Crystal Violet -->
  
−
<h2>Crystal Violet</h2>
+
<h2 id="Crystal-Violet">Crystal Violet</h2>
 
<p><u>Use:</u> Marker for bead quality assay</p>
 
<p><u>Use:</u> Marker for bead quality assay</p>
 
<p>u>Signal word:</u> Danger</p>
 
<p>u>Signal word:</u> Danger</p>
Line 273: Line 300:
 
<!-- Lysogeny Broth -->
 
<!-- Lysogeny Broth -->
  
−
<h2>Lysogeny Broth</h2>
+
<h2 id="Lysogeny-Broth">Lysogeny Broth</h2>
 
<p><u>Use:</u> Media for general bacterial growth</p>
 
<p><u>Use:</u> Media for general bacterial growth</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
Line 289: Line 316:
 
<!-- SDS -->
 
<!-- SDS -->
  
−
<h2>Sodium Dodecyl Sulphate</h2>
+
<h2 id="Sodium-Dodecyl-Sulfate">Sodium Dodecyl Sulphate</h2>
 
<p><u>Use:</u> Detergent in protein identification gels</p>
 
<p><u>Use:</u> Detergent in protein identification gels</p>
 
<p>u>Signal word:</u> Danger</p>
 
<p>u>Signal word:</u> Danger</p>
Line 316: Line 343:
 
<!-- Ethanol -->
 
<!-- Ethanol -->
  
−
<h2>Ethanol 100%</h2>
+
<h2 id="Ethanol">Ethanol 100%</h2>
 
<p><u>Use:</u> solvent for chloramphenicol</p>
 
<p><u>Use:</u> solvent for chloramphenicol</p>
 
<p>u>Signal word:</u> Danger</p>
 
<p>u>Signal word:</u> Danger</p>
Line 337: Line 364:
 
<!-- TFB I-->
 
<!-- TFB I-->
  
−
<h2>Transfection Buffer I (Manganese[II] Chloride)</h2>
+
<h2 id="Transfection-Buffer-I">Transfection Buffer I (Manganese[II] Chloride)</h2>
 
<p><u>Use:</u> First soaking buffer in creating competent E. coli cells</p>
 
<p><u>Use:</u> First soaking buffer in creating competent E. coli cells</p>
 
<p>u>Signal word:</u> Danger</p>
 
<p>u>Signal word:</u> Danger</p>
Line 362: Line 389:
 
<!-- TFB II -->
 
<!-- TFB II -->
  
−
<h2>Transfection Buffer II</h2>
+
<h2 id="Transfection-Buffer-II">Transfection Buffer II</h2>
 
<p><u>Use:</u> Second soaking buffer in creating competent E. coli cells</p>
 
<p><u>Use:</u> Second soaking buffer in creating competent E. coli cells</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
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<!--Chitosan-->
 
<!--Chitosan-->
  
−
<h2>Chitosan</h2>
+
<h2 id="Chitosan">Chitosan</h2>
 
<p><u>Use:</u> Coating for bacterial delivery beads</p>
 
<p><u>Use:</u> Coating for bacterial delivery beads</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
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<!-- Instant Blue -->
 
<!-- Instant Blue -->
  
−
<h2>InstantBlue</h2>
+
<h2 id="Instant-Blue">InstantBlue</h2>
 
<p><u>Use:</u> Protein stain for protein identification gels</p>
 
<p><u>Use:</u> Protein stain for protein identification gels</p>
 
<p>u>Signal word:</u> Warning</p>
 
<p>u>Signal word:</u> Warning</p>
Line 393: Line 420:
 
</ul>
 
</ul>
  
−
<!-- Purple Loding Dye -->
+
<!-- Purple Loading Dye -->
  
−
<h2>NEB Purple Loading Dye</h2>
+
<h2 id="NEB-Purple-Loading-Dye">NEB Purple Loading Dye</h2>
 
<p><u>Use:</u> Stabilizer and marker for DNA gel electrophoresis</p>
 
<p><u>Use:</u> Stabilizer and marker for DNA gel electrophoresis</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
Line 403: Line 430:
 
<!-- Protease Inhibitors -->
 
<!-- Protease Inhibitors -->
  
−
<h2>Roche cOmplete EDTA-free Protease Inhibitor Cocktail</h2>
+
<h2 id="Protease-Inhibitors">Roche cOmplete EDTA-free Protease Inhibitor Cocktail</h2>
 
<p><u>Use:</u> Prevention of protein cleavage during protein purification</p>
 
<p><u>Use:</u> Prevention of protein cleavage during protein purification</p>
 
<p>u>Signal word:</u> Danger</p>
 
<p>u>Signal word:</u> Danger</p>
Line 425: Line 452:
 
<!-- HCL -->
 
<!-- HCL -->
 
<section>
 
<section>
−
<h2>Hydrochloric Acid 1M</h2>
+
<h2 id="Hydrochloric-Acid">Hydrochloric Acid 1M</h2>
 
<p><u>Use:</u> Simulation of stomach acid conditions in bead experiments</p>
 
<p><u>Use:</u> Simulation of stomach acid conditions in bead experiments</p>
 
<p>u>Signal word:</u> Warning</p>
 
<p>u>Signal word:</u> Warning</p>
Line 440: Line 467:
 
<!-- NaOH -->
 
<!-- NaOH -->
  
−
<h2>Sodium Hydroxide</h2>
+
<h2 id="Sodium-Hydroxide">Sodium Hydroxide</h2>
 
<p><u>Use:</u> Simulation of duodenum conditions in bead experiments</p>
 
<p><u>Use:</u> Simulation of duodenum conditions in bead experiments</p>
 
<p>u>Signal word:</u> Danger</p>
 
<p>u>Signal word:</u> Danger</p>
Line 463: Line 490:
 
<!-- Monobasic Sodium Phopshate -->
 
<!-- Monobasic Sodium Phopshate -->
  
−
<h2>Monobasic Sodium Phosphate</h2>
+
<h2 id="Monobasic-Sodium-Phosphate">Monobasic Sodium Phosphate</h2>
 
<p><u>Use:</u> Simulation of duodenum conditions in bead experiments</p>
 
<p><u>Use:</u> Simulation of duodenum conditions in bead experiments</p>
 
<p>Non-Hazardous</p>
 
<p>Non-Hazardous</p>
Line 472: Line 499:
 
<!-- Glacial Acetic ACid -->
 
<!-- Glacial Acetic ACid -->
  
−
<h2>Glacial Acetic Acid</h2>
+
<h2 id="Glacial-Acetic-Acid">Glacial Acetic Acid</h2>
 
<p><u>Use:</u> Bead experiments</p>
 
<p><u>Use:</u> Bead experiments</p>
 
<p>u>Signal word:</u> Danger</p>
 
<p>u>Signal word:</u> Danger</p>
Line 495: Line 522:
 
<!-- CaCl -->
 
<!-- CaCl -->
  
−
<h2>Calcium Chloride</h2>
+
<h2 id="Calcium-Chloride">Calcium Chloride</h2>
 
<p><u>Use:</u> Forming beads from alginate.  Ca2+ causes alginate phase change</p>
 
<p><u>Use:</u> Forming beads from alginate.  Ca2+ causes alginate phase change</p>
 
<p>u>Signal word:</u> Warning</p>
 
<p>u>Signal word:</u> Warning</p>

Revision as of 20:40, 16 October 2016

iGEM Oxford 2016 - Cure for Copper

Chemicals

Ethidium Bromide

Use: Staining DNA electrophoresis gels.

Signal word: Danger


Hazard statement(s)

  • H302 Harmful if swallowed.
  • H330 Fatal if inhaled.
  • H341 Suspected of causing genetic defects.

Precautionary statement(s)

  • P260 Do not breathe dust/fume/gas/mist/vapours/spray.
  • P281 Use personal protective equipment as required.
  • P284 Wear respiratory protection.
  • P310 Immediately call a POISON CENTER or doctor/ physician.

TBE Buffer pH 8.3

Use: General buffer for DNA experiments.
Made of three chemicals: Tris,borate and EDTA.

Non-Hazardous

Copper Sulphate Pentahydrate:

Use: Induction of copper promoters and Cu2+ chelation assays.

u>Signal word: Warning

Hazard statement(s)

  • H302 Harmful if swallowed.
  • H315 Causes skin irritation.
  • H319 Causes serious eye irritation.
  • H410 Very toxic to aquatic life with long lasting effects.

Precautionary statement(s)

  • P273 Avoid release to the environment.
  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes.Remove contact lenses, if present and easy to do. Continue rinsing.
  • P501 Dispose of contents/container to an approved waste disposal plant.

Agarose

Use: DNA electrophoresis gel matrix

Non-Hazardous

L(+)-Arabinose

Use: Induction of pBAD expression

Non-Hazardous

Bathocuproinedisulfonic Acid Disodium Salt

Use: Chromophore in Cu+/Cu2+ detection assay

Non-Hazardous

L(+)-Sodium Ascorbate

Use: Reducing agent in Cu+/Cu2+ detection assay

Non-Hazardous

L-Glutathione

Use: Reducing agent in Cu+/Cu2+ detection assay.

Non-Hazardous

Chloramphenicol

Use: Antibiotic and positive selection marker for all plasmids except pBAD

u>Signal word: Danger

Hazard statement(s)

  • H350 May cause cancer

Precautionary statement(s)

  • P201 Obtain special instructions before use.
  • P308 + P313 If exposed or concerned: Get medical advice/attention.

Ampicillin

Use: Antibiotic and positive selection marker for pBAD.

u>Signal word: Danger

Hazard statement(s)

  • H315 Causes skin irritation
  • H317 May cause an allergic skin reaction
  • H319 Causes serious eye irritation
  • H334 May cause allergy or asthma symptoms or breathing difficulties if inhaled
  • H335 May cause respiratory irritation

Precautionary statement(s)

  • P261 Avoid breathing dust/fume/gas/mist/vapours/spray
  • P280 Wear protective gloves
  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
  • P342 + P311 If experiencing respiratory symptoms: Call a POISON CENTER or doctor/physician

Tris pH 7.0 and 8.0

Use: General buffer for protein and copper assays

Non-Hazardous

Glycerol

Use: Component of protein buffer for protein stability at low temperatures. Used in protein purification lysis and elution buffers. Also, used in SDS-PAGE run buffer

Non-Hazardous

DL-Dithiothreitol

Use: Reducing agent in Cu+/Cu2+ detection assay and protein purification

u>Signal word: Warning

Hazard statement(s)

  • H302 Harmful if swallowed
  • H315 Causes skin irritation
  • H319 Causes serious eye irritation
  • H335 May cause respiratory irritation

Precautionary statement(s)

  • P261 Avoid breathing dust
  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing

Imidazole

Use: Elution of His-tagged target proteins from nickel bead column during protein purification

u>Signal word: Danger

Hazard statement(s)

  • H302 Harmful if swallowed
  • H314 Causes severe skin burns and eye damage
  • H360D May damage the unborn child

Precautionary statement(s)

  • P201 Obtain special instructions before use
  • P280 Wear protective gloves/protective clothing/eye protection/face protection
  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
  • P310 Immediately call a POISON CENTER or doctor/physician

Nickel Affinity Beads

Use: Selection of His-tagged target proteins during protein purification

Non-Hazardous

Sodium Alginate

Use: Major component of bacterial delivery beads

Non-Hazardous

Crystal Violet

Use: Marker for bead quality assay

u>Signal word: Danger

Hazard statement(s)

  • H302 Harmful if swallowed
  • H318 Causes serious eye damage
  • H350 May cause cancer
  • H410 Very toxic to aquatic life with long lasting effects

Precautionary statement(s)

  • P201 Obtain special instructions before use
  • P273 Avoid release to the environment
  • P280 Wear protective gloves/ eye protection/ face protection
  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
  • P308 + P313 If exposed or concerned: Get medical advice/attention
  • P501 Dispose of contents/container to an approved waste disposal plant

Lysogeny Broth

Use: Media for general bacterial growth

Non-Hazardous

Phosphate Buffered Saline pH 7.4

Use: Dilution buffer for flow cytometry experiments

Non-Hazardous

Sodium Dodecyl Sulphate

Use: Detergent in protein identification gels

u>Signal word: Danger

Hazard statement(s)

  • H228 Flammable solid
  • H302 + H332 Harmful if swallowed or if inhaled
  • H315 Causes skin irritation
  • H318 Causes serious eye damage
  • H335 May cause respiratory irritation
  • H412 Harmful to aquatic life with long lasting effects

Precautionary statement(s)

  • P210 Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking
  • P261 Avoid breathing dust/fume/gas/mist/vapours/spray
  • P280 Wear eye protection/face protection
  • P301 + P312 + P330 IF SWALLOWED: Call a POISON CENTER/doctor if you feel unwell. Rinse mouth
  • P305 + P351 + P338 + P310 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER/doctor
  • P370 + P378 In case of fire: Use dry powder or dry sand to extinguish

Ethanol 100%

Use: solvent for chloramphenicol

u>Signal word: Danger

Hazard statement(s)

  • H225 Highly flammable liquid and vapour
  • H319 Causes serious eye irritation

Precautionary statement(s)

  • P210 Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking
  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
  • P370 + P378 In case of fire: Use dry powder or dry sand to extinguish
  • P403 + P235 Store in a well-ventilated place. Keep cool

Transfection Buffer I (Manganese[II] Chloride)

Use: First soaking buffer in creating competent E. coli cells

u>Signal word: Danger

Hazard statement(s)

  • H302 Harmful if swallowed
  • H318 Causes serious eye damage
  • H373 May cause damage to organs (Brain) through prolonged or repeated exposure if inhaled
  • H411 Toxic to aquatic life with long lasting effects

Precautionary statement(s)

  • P273 Avoid release to the environment
  • P280 Wear eye protection/ face protection
  • P301 + P312 + P330 IF SWALLOWED: Call a POISON CENTER or doctor/physician if you feel unwell. Rinse mouth
  • P305 + P351 + P338 + P310 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER or doctor/physician
  • P391 Collect spillage
  • P501 Dispose of contents/container to an approved waste disposal plant

Transfection Buffer II

Use: Second soaking buffer in creating competent E. coli cells

Non-Hazardous

Chitosan

Use: Coating for bacterial delivery beads

Non-Hazardous

InstantBlue

Use: Protein stain for protein identification gels

u>Signal word: Warning

Hazard statement(s)

  • H290 May be corrosive to metals
  • H315 Causes skin irritation
  • H319 Causes serious eye irritation

Precautionary statement(s)

  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing

NEB Purple Loading Dye

Use: Stabilizer and marker for DNA gel electrophoresis

Non-Hazardous

Roche cOmplete EDTA-free Protease Inhibitor Cocktail

Use: Prevention of protein cleavage during protein purification

u>Signal word: Danger

Hazard statement(s)

  • H314 Causes severe skin burns and eye damage Skin corrosion/irritation

Precautionary statement(s)

  • P260 Do not breathe dust/fume/gas/mist/vapours/spray
  • P280 Wear protective gloves/protective clothing/eye protection/face protection
  • P301 + P330 + P331 IF SWALLOWED: Rinse mouth. Do NOT induce vomiting
  • P303 + P361 + P353 IF ON SKIN (or hair): Remove/Take off Immediately all contaminated clothing. Rinse SKIN with water/shower
  • P304 + P340 + P310 IF INHALED: Remove victim to fresh air and Keep at rest in a position comfortable for breathing. Immediately call a POISON CENTER or doctor/physician
  • P305 + P351 + P338 + P310 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Immediately call a POISON CENTER or doctor/physician

Hydrochloric Acid 1M

Use: Simulation of stomach acid conditions in bead experiments

u>Signal word: Warning

Hazard statement(s)

  • H290 May be corrosive to metals

Sodium Hydroxide

Use: Simulation of duodenum conditions in bead experiments

u>Signal word: Danger

Hazard statement(s)

  • H290 May be corrosive to metals
  • H314 Causes severe skin burns and eye damage

Precautionary statement(s)

  • P260 Do not breathe dust/fume/gas/mist/vapours/spray
  • P280 Wear protective gloves/protective clothing/eye protection/face protection
  • P303 + P361 + P353 IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/shower
  • P304 + P340 + P310 IF INHALED: Remove person to fresh air and keep comfortable for breathing. Immediately call a POISON CENTER/doctor
  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing

Monobasic Sodium Phosphate

Use: Simulation of duodenum conditions in bead experiments

Non-Hazardous

Glacial Acetic Acid

Use: Bead experiments

u>Signal word: Danger

Hazard statement(s)

  • H226 Flammable liquid and vapour
  • H314 Causes severe skin burns and eye damage

Precautionary statement(s)

  • P210 Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking
  • P260 Do not breathe dust/fume/gas/mist/vapours/spray
  • P280 Wear protective gloves/protective clothing/eye protection/face protection
  • P303 + P361 + P353 IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/shower
  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
  • P370 + P378 In case of fire: Use dry powder or dry sand to extinguish

Calcium Chloride

Use: Forming beads from alginate. Ca2+ causes alginate phase change

u>Signal word: Warning

Hazard statement(s)

  • H319 Causes serious eye irritation

Precautionary statement(s)

  • P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing